Restriction map for pF1KA1008
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspI        AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BglI        BlnI        BsaAI       
BsaHI       BsaWI       BseAI       BseRI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      Bst1107I    
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoT22I     
FbaI        HaeII       Hin1I       HpaI        KasI        
KpnI        KspI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NarI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SnaBI       Sse8387I    
SspBI       StuI        SwaI        Tth111I     XbaI        
XhoI        


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# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         1342  GTAGAC               GT^[AC][GT]AC
AccII          61  CGCG                 CG^CG
AlwNI         171  CAGCGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1317  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1697  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          64  GAGCAC               G[AT]GC[AT]^C
AviII         106  TGCGCA               TGC^GCA
BglII        1857  AGATCT               A^GATCT
BmyI           64  GAGCAC               G[AGT]GC[ACT]^C
BsaI          969  GAGACC               GGTCTC(1/5)
BsgI          220  CTGCAC               GTGCAG(16/14)
BsiHKAI        64  GAGCAC               G[AT]GC[AT]^C
Bsp1286I       64  GAGCAC               G[AGT]GC[ACT]^C
BspHI        1944  TCATGA               T^CATGA
BsrFI         135  GCCGGC               [GA]^CCGG[CT]
BsrI         2418  CCAGT                ACTGG(1/-1)
BssSI         127  CACGAG               CACGAG(-5/-1)
BstBI        1973  TTCGAA               TT^CGAA
BstUI          61  CGCG                 CG^CG
BstXI        2063  CCAAGTCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1317  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        135  GCCGGC               [GA]^CCGG[CT]
DraI          756  TTTAAA               TTT^AAA
DraIII       1153  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1317  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1055  GAATTC               G^AATTC
EcoRV        1139  GATATC               GAT^ATC
FseI          134  GGCCGGCC             GGCCGG^CC
FspI          106  TGCGCA               TGC^GCA
HincII       2319  GTTGAC               GT[CT]^[GA]AC
HindII       2319  GTTGAC               GT[CT]^[GA]AC
MspA1I        412  CAGCTG               C[AC]G^C[GT]G
MvnI           61  CGCG                 CG^CG
NaeI          135  GCCGGC               GCC^GGC
NcoI         1892  CCATGG               C^CATGG
NdeI         1676  CATATG               CA^TATG
NgoMIV        135  GCCGGC               G^CCGGC
NheI          667  GCTAGC               G^CTAGC
NspV         1973  TTCGAA               TT^CGAA
PstI         1861  CTGCAG               CTGCA^G
PvuII         412  CAGCTG               CAG^CTG
RcaI         1944  TCATGA               T^CATGA
SfuI         1973  TTCGAA               TT^CGAA
SpeI         1732  ACTAGT               A^CTAGT
SphI          983  GCATGC               GCATG^C
Tsp45I       1568  GTGAC                ^GT[GC]AC
XcmI         1267  CCAAAGATGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1697  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AflII         609  CTTAAG               C^TTAAG
              898  CTTAAG              
AseI         1654  ATTAAT               AT^TAAT
             2496  ATTAAT              
AsnI         1654  ATTAAT               AT^TAAT
             2496  ATTAAT              
AvaI           32  CCCGGG               C^[CT]CG[GA]G
              100  CCCGGG              
BbsI         1016  GAAGAC               GAAGAC(2/6)
             1312  GAAGAC              
BfrI          609  CTTAAG               C^TTAAG
              898  CTTAAG              
BlpI         1539  GCTTAGC              GC^T[ACGT]AGC
             2207  GCTTAGC             
Bpu1102I     1539  GCTTAGC              GC^T[ACGT]AGC
             2207  GCTTAGC             
BpuAI        1016  GAAGAC               GAAGAC(2/6)
             1312  GAAGAC              
BsaBI          48  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2191  GATTTTCATC          
BsmBI        2213  CGTCTC               CGTCTC(1/5)
               77  GAGACG              
BsoBI          32  CCCGGG               C^[CT]CG[GA]G
              100  CCCGGG              
BspMI          71  ACCTGC               ACCTGC(4/8)
             1032  ACCTGC              
BstF5I       1981  CATCC                GGATG(2/0)
             1354  GGATG               
BstYI        1857  AGATCT               [GA]^GATC[CT]
             2675  AGATCC              
CelII        1539  GCTTAGC              GC^T[ACGT]AGC
             2207  GCTTAGC             
DraII         158  AGGACCC              [GA]G^G[ACGT]CC[CT]
              250  GGGACCT             
DsaI         1740  CCGTGG               C^C[GA][CT]GG
             1892  CCATGG              
EarI          880  GAAGAG               CTCTTC(1/4)
             1484  GAAGAG              
EcoO109I      158  AGGACCC              [GA]G^G[ACGT]CC[CT]
              250  GGGACCT             
FauI          180  CCCGC                CCCGC(4/6)
               37  GCGGG               
FokI         1981  CATCC                GGATG(9/13)
             1354  GGATG               
HgaI          173  GCGTC                GACGC(5/10)
             2212  GCGTC               
HindIII      2335  AAGCTT               A^AGCTT
             2773  AAGCTT              
HphI         1171  GGTGA                GGTGA(8/7)
             2239  TCACC               
Ksp632I       880  GAAGAG               CTCTTC(1/4)
             1484  GAAGAG              
MamI           48  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2191  GATTTTCATC          
MflI         1857  AGATCT               [GA]^GATC[CT]
             2675  AGATCC              
NspI          983  GCATGC               [GA]CATG^[CT]
             1962  ACATGC              
PflMI         973  CCATATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2503  CCAAAGTATGG         
PpuMI         158  AGGACCC              [GA]G^G[AT]CC[CT]
              250  GGGACCT             
PshBI        1654  ATTAAT               AT^TAAT
             2496  ATTAAT              
SmaI           32  CCCGGG               CCC^GGG
              100  CCCGGG              
Van91I        973  CCATATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2503  CCAAAGTATGG         
XhoII        1857  AGATCT               [GA]^GATC[CT]
             2675  AGATCC              
XmaCI          32  CCCGGG               C^CCGGG
              100  CCCGGG              
XmaI           32  CCCGGG               C^CCGGG
              100  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbvI        BcnI        BfaI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsmI        BssKI       BstAPI      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     
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