Restriction map for pF1KA1008
 (Circular) (Six-base) MAPSORT of: pF1KA1008.seq  Check: 1695  from: 1  to: 5897



pF1KA1008  5897 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1149    1149 
   Size:       5897
 
AccI GT'mk_AC

Cuts at:   1451    2935    1451 
   Size:       1484    4413
 
Acc65I G'GTAC_C

Cuts at:   2913    2913 
   Size:       5897
 
AclI AA'CG_TT

Cuts at:   5737    5737 
   Size:       5897
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    683    1811    1897    1943    3722    4154    4901    5169     683 
   Size:       1128      86      46    1779     432     747     268    1411

  Fragments arranged by size: 

                1779    1411    1128     747     432     268      86      46
            
 
AflII C'TTAA_G

Cuts at:    717    1006     717 
   Size:        289    5608
 
AflIII A'CryG_T

Cuts at:   3249    4353    5330    3249 
   Size:       1104     977    3816

  Fragments arranged by size: 

                3816    1104     977
 
AgeI A'CCGG_T

Cuts at:   4285    4285 
   Size:       5897
 
AleI CACnn'nnGTG

Cuts at:    319     319 
   Size:       5897
 
AlwNI CAG_nnn'CTG

Cuts at:    284    3202    4769     284 
   Size:       2918    1567    1412

  Fragments arranged by size: 

                2918    1567    1412
 
ApaLI G'TGCA_C

Cuts at:   4667    4667 
   Size:       5897
 
ApoI r'AATT_y

Cuts at:   1163    1239    1272    1512    1871    1995    2011    2048    2059 
   Size:         76      33     240     359     124      16      37      11

Cuts at:   2059    2117    2145    2469    2901    5250    5506    1163 
   Size:         58      28     324     432    2349     256    1554

  Fragments arranged by size: 

                2349    1554     432     359     324     256     240     124
                  76      58      37      33      28      16      11
 
AseI AT'TA_AT

Cuts at:     20    1763    2605      20 
   Size:       1743     842    3312

  Fragments arranged by size: 

                3312    1743     842
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5897
 
AvaI C'yCGr_G

Cuts at:    140     208    2917     140 
   Size:         68    2709    3120

  Fragments arranged by size: 

                3120    2709      68
 
BamHI G'GATC_C

Cuts at:   2922    2922 
   Size:       5897
 
BanI G'GyrC_C

Cuts at:   2913    3327    3575    3610    2913 
   Size:        414     248      35    5200

  Fragments arranged by size: 

                5200     414     248      35
 
BanII G_rGCy'C

Cuts at:   2911    3941    2911 
   Size:       1030    4867
 
BbeI G_GCGC'C

Cuts at:   3331    3579    3331 
   Size:        248    5649
 
BbsI GAAGACnn'nnnn_

Cuts at:   1131    1427    1131 
   Size:        296    5601
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    5863
 
BciVI GTATCCnnnnn_n'

Cuts at:   3795    4556    5897    3795 
   Size:        761    1341    3795

  Fragments arranged by size: 

                3795    1341     761
 
BclI T'GATC_A

Cuts at:   3417    3417 
   Size:       5897
 
BglII A'GATC_T

Cuts at:   1965    3412    5449    1965 
   Size:       1447    2037    2413

  Fragments arranged by size: 

                2413    2037    1447
 
BlpI GC'TnA_GC

Cuts at:   1648    2316    3009    1648 
   Size:        668     693    4536

  Fragments arranged by size: 

                4536     693     668
 
Bme1580I G_kGCm'C

Cuts at:   3522    3615    4671    3522 
   Size:         93    1056    4748

  Fragments arranged by size: 

                4748    1056      93
 
BmgBI CAC'GTC

Cuts at:   1680    1680 
   Size:       5897
 
BmrI ACTGGGnnnn_n'

Cuts at:   2520    3109    3190    3525    5669    2520 
   Size:        589      81     335    2144    2748

  Fragments arranged by size: 

                2748    2144     589     335      81
 
BmtI G_CTAG'C

Cuts at:    779     779 
   Size:       5897
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1175    2709    4264    1175 
   Size:       1534    1555    2808

  Fragments arranged by size: 

                2808    1555    1534
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    271     363     369    1407     271 
   Size:         92       6    1038    4761

  Fragments arranged by size: 

                4761    1038      92       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:     97    3073    3952    4444    4742      97 
   Size:       2976     879     492     298    1252

  Fragments arranged by size: 

                2976    1252     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1071    5601    1071 
   Size:       4530    1367
 
BsaAI yAC'GTr

Cuts at:   3250    3250 
   Size:       5897
 
BsaBI GATnn'nnATC

Cuts at:      6     160    2303    3411    5448       6 
   Size:        154    2143    1108    2037     455

  Fragments arranged by size: 

                2143    2037    1108     455     154
 
BsaHI Gr'CG_yC

Cuts at:   3328    3576    4278    5774    3328 
   Size:        248     702    1496    3451

  Fragments arranged by size: 

                3451    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   3086    3607    4285    4559    4706    5117    3086 
   Size:        521     678     274     147     411    3866

  Fragments arranged by size: 

                3866     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   3322    4080    4657    5153    3322 
   Size:        758     577     496    4066

  Fragments arranged by size: 

                4066     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    313     313 
   Size:       5897
 
BsiEI CG_ry'CG

Cuts at:    104    3439    3485    4693     104 
   Size:       3335      46    1208    1308

  Fragments arranged by size: 

                3335    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    176    2911    3689    3879    4671     176 
   Size:       2735     778     190     792    1402

  Fragments arranged by size: 

                2735    1402     792     778     190
 
BsmI GAATG_Cn'

Cuts at:   1007    2595    2825    1007 
   Size:       1588     230    4079

  Fragments arranged by size: 

                4079    1588     230
 
BsmBI CGTCTCn'nnnn_

Cuts at:    179    2327     179 
   Size:       2148    3749
 
Bsp1286I G_dGCh'C

Cuts at:    176    2911    3522    3615    3689    3879    3941    4671     176 
   Size:       2735     611      93      74     190      62     730    1402

  Fragments arranged by size: 

                2735    1402     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   5117    5117 
   Size:       5897
 
BspHI T'CATG_A

Cuts at:   2052    5073    2052 
   Size:       3021    2876
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    188    1149    2947    3161    3463    3844     188 
   Size:        961    1798     214     302     381    2241

  Fragments arranged by size: 

                2241    1798     961     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    4189    4243    5727    5892      50 
   Size:       4139      54    1484     165      55

  Fragments arranged by size: 

                4139    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3809    3809 
   Size:       5897
 
BsrFI r'CCGG_y

Cuts at:    243    3895    4076    4285     243 
   Size:       3652     181     209    1855

  Fragments arranged by size: 

                3652    1855     209     181
 
BssSI C'ACGA_G

Cuts at:    235    4168    4526     235 
   Size:       3933     358    1606

  Fragments arranged by size: 

                3933    1606     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    180     284     303    3274     180 
   Size:        104      19    2971    2803

  Fragments arranged by size: 

                2971    2803     104      19
 
BstBI TT'CG_AA

Cuts at:   2082    4258    2082 
   Size:       2176    3721
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2178    2178 
   Size:       5897
 
BstYI r'GATC_y

Cuts at:      1    1965    2783    2922    3389    3412    3747    3993    5005 
   Size:       1964     818     139     467      23     335     246    1012

Cuts at:   5005    5091    5103    5449       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1964    1012     818     467     449     346     335     246
                 139      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1426    1426 
   Size:       5897
 
BtgI C'CryG_G

Cuts at:   1848    2000    1848 
   Size:        152    5745
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3847    4028    4191    3847 
   Size:        181     163    5553

  Fragments arranged by size: 

                5553     181     163
 
BtsI GCAGTG_nn'

Cuts at:   3140    3140 
   Size:       5897
 
DraI TTT'AAA

Cuts at:    866    2896     866 
   Size:       2030    3867
 
DraIII CAC_nnn'GTG

Cuts at:   1266    1266 
   Size:       5897
 
DrdI GACnn_nn'nnGTC

Cuts at:   3603    4461    3603 
   Size:        858    5039
 
EaeI y'GGCC_r

Cuts at:   3482    3656    4047    4074    5419    3482 
   Size:        174     391      27    1345    3960

  Fragments arranged by size: 

                3960    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   3482    3482 
   Size:       5897
 
EarI CTCTTCn'nnn_

Cuts at:    983    1587    3920    4130     983 
   Size:        604    2333     210    2750

  Fragments arranged by size: 

                2750    2333     604     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4105    4415    4561    5707    4105 
   Size:        310     146    1146    4295

  Fragments arranged by size: 

                4295    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2909    2909 
   Size:       5897
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    271     363     369     683    1407    1811    1897    1943    3722 
   Size:         92       6     314     724     404      86      46    1779

Cuts at:   3722    4154    4901    5169     271 
   Size:        432     747     268     999

  Fragments arranged by size: 

                1779     999     747     724     432     404     314     268
                  92      86      46       6
 
EcoO109I rG'GnC_Cy

Cuts at:    267     359    3036     267 
   Size:         92    2677    3128

  Fragments arranged by size: 

                3128    2677      92
 
EcoRI G'AATT_C

Cuts at:   1163    2901    1163 
   Size:       1738    4159
 
EcoRV GAT'ATC

Cuts at:   1249    1249 
   Size:       5897
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    144    3916     144 
   Size:       3772    2125
 
FseI GG_CCGG'CC

Cuts at:    247     247 
   Size:       5897
 
FspI TGC'GCA

Cuts at:    216    3678     216 
   Size:       3462    2435
 
HaeII r_GCGC'y

Cuts at:   3331    3579    4601    5580    3331 
   Size:        248    1022     979    3648

  Fragments arranged by size: 

                3648    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    176     208    1567    1599    3739    3769    3771    3801     176 
   Size:         32    1359      32    2140      30       2      30    2272

  Fragments arranged by size: 

                2272    2140    1359      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   2429    2936    2429 
   Size:        507    5390
 
HindIII A'AGCT_T

Cuts at:   2443    2881    2443 
   Size:        438    5459
 
KasI G'GCGC_C

Cuts at:   3327    3575    3327 
   Size:        248    5649
 
KpnI G_GTAC'C

Cuts at:   2917    2917 
   Size:       5897
 
MluI A'CGCG_T

Cuts at:   5330    5330 
   Size:       5897
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1266    4568    4752    1266 
   Size:       3302     184    2411

  Fragments arranged by size: 

                3302    2411     184
 
MscI TGG'CCA

Cuts at:   3658    3658 
   Size:       5897
 
MslI CAynn'nnrTG

Cuts at:    319    1461    1698    2309    3886    4013     319 
   Size:       1142     237     611    1577     127    2203

  Fragments arranged by size: 

                2203    1577    1142     611     237     127
 
MspA1I CmG'CkG

Cuts at:    522    3008    3322    3682    4695    4940     522 
   Size:       2486     314     360    1013     245    1479

  Fragments arranged by size: 

                2486    1479    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:    245    4078     245 
   Size:       3833    2064
 
NarI GG'CG_CC

Cuts at:   3328    3576    3328 
   Size:        248    5649
 
NcoI C'CATG_G

Cuts at:   2000    2000 
   Size:       5897
 
NdeI CA'TA_TG

Cuts at:   1785    1785 
   Size:       5897
 
NgoMIV G'CCGG_C

Cuts at:    243    4076     243 
   Size:       3833    2064
 
NheI G'CTAG_C

Cuts at:    775     775 
   Size:       5897
 
NspI r_CATG'y

Cuts at:   1095    2074    2950    3450    4357    1095 
   Size:        979     876     500     907    2635

  Fragments arranged by size: 

                2635     979     907     876     500
 
PciI A'CATG_T

Cuts at:   4353    4353 
   Size:       5897
 
PflMI CCAn_nnn'nTGG

Cuts at:   1087    2617    1087 
   Size:       1530    4367
 
PmeI GTTT'AAAC

Cuts at:   2896    2896 
   Size:       5897
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5036    5068    5036 
   Size:         32    5865
 
PpuMI rG'GwC_Cy

Cuts at:    267     359     267 
   Size:         92    5805
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2795    2827    2795 
   Size:         32    5865
 
PstI C_TGCA'G

Cuts at:   1973    2944    3629    1973 
   Size:        971     685    4241

  Fragments arranged by size: 

                4241     971     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5897
 
PvuII CAG'CTG

Cuts at:    522    3322    3682     522 
   Size:       2800     360    2737

  Fragments arranged by size: 

                2800    2737     360
 
RsrII CG'GwC_CG

Cuts at:   4092    4092 
   Size:       5897
 
SacI G_AGCT'C

Cuts at:   2911    2911 
   Size:       5897
 
SalI G'TCGA_C

Cuts at:   2934    2934 
   Size:       5897
 
SapI GCTCTTCn'nnn_

Cuts at:   3920    4130    3920 
   Size:        210    5687
 
SbfI CC_TGCA'GG

Cuts at:   2944    2944 
   Size:       5897
 
ScaI AGT'ACT

Cuts at:   5414    5414 
   Size:       5897
 
SfcI C'TryA_G

Cuts at:     33     727     800    1040    1961    1969    2940    3625    4618 
   Size:        694      73     240     921       8     971     685     993

Cuts at:   4618    4809      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     971     921     694     685     240     191
                  73       8
 
SfoI GGC'GCC

Cuts at:   3329    3577    3329 
   Size:        248    5649
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5897
 
SmaI CCC'GGG

Cuts at:    142     210    2919     142 
   Size:         68    2709    3120

  Fragments arranged by size: 

                3120    2709      68
 
SmlI C'TyrA_G

Cuts at:    112     717    1006    3052    3967    4459    4721     112 
   Size:        605     289    2046     915     492     262    1288

  Fragments arranged by size: 

                2046    1288     915     605     492     289     262
 
SpeI A'CTAG_T

Cuts at:     67    1840      67 
   Size:       1773    4124
 
SphI G_CATG'C

Cuts at:   1095    2950    3450    1095 
   Size:       1855     500    3542

  Fragments arranged by size: 

                3542    1855     500
 
SspI AAT'ATT

Cuts at:    698    2031    2243     698 
   Size:       1333     212    4352

  Fragments arranged by size: 

                4352    1333     212
 
StyI C'CwwG_G

Cuts at:   1571    1976    2000    3031    3385    1571 
   Size:        405      24    1031     354    4083

  Fragments arranged by size: 

                4083    1031     405     354      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2364    3480    3613    4072    4281    2364 
   Size:       1116     133     459     209    3980

  Fragments arranged by size: 

                3980    1116     459     209     133
 
TatI w'GTAC_w

Cuts at:   1465    2273    2413    5412    1465 
   Size:        808     140    2999    1950

  Fragments arranged by size: 

                2999    1950     808     140
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1250    1282    1589    1621    5414    5446    1250 
   Size:         32     307      32    3793      32    1701

  Fragments arranged by size: 

                3793    1701     307      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3694    3694 
   Size:       5897
 
XbaI T'CTAG_A

Cuts at:   2928    2928 
   Size:       5897
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1382    3392    1382 
   Size:       2010    3887
 
XmaI C'CCGG_G

Cuts at:    140     208    2917     140 
   Size:         68    2709    3120

  Fragments arranged by size: 

                3120    2709      68
 
XmnI GAAnn'nnTTC

Cuts at:   1809    1809 
   Size:       5897
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NspI     PciI    PflMI     PmeI     PpiI
    PpuMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AlfI     AloI     ApaI     AscI    AvrII
     BaeI    BbvCI    BfrBI     BglI   Bpu10I    BsaXI    BseRI    BsiWI
    BsrGI   BssHII   BstEII  BstZ17I     ClaI    CspCI    EcoNI    FspAI
     HpaI     MfeI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmlI    PshAI     PsiI   PspOMI    PspXI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI     ZraI
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