Restriction map for pF1KA0910
 (Linear) (Six-base) MAPSORT of: KIAA0910.seq  Check: 6249  from: 1  to: 3837



KIAA0910.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     846    3837 
   Size:        846    2991
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     314    1828    2483    2581    2645    3080    3323 
   Size:        299      15    1514     655      98      64     435     243

Cuts at:   3323    3646    3779    3837 
   Size:        323     133      58

  Fragments arranged by size: 

                1514     655     435     323     299     243     133      98
                  64      58      15
 
AflII C'TTAA_G

Cuts at:      0     865    3837 
   Size:        865    2972
 
AflIII A'CryG_T

Cuts at:      0    2516    3837 
   Size:       2516    1321
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2554    2780    2983    3837 
   Size:       2554     226     203     854

  Fragments arranged by size: 

                2554     854     226     203
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1094    1126    3837 
   Size:       1094      32    2711

  Fragments arranged by size: 

                2711    1094      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1327    3466    3837 
   Size:       1327    2139     371

  Fragments arranged by size: 

                2139    1327     371
 
ApaLI G'TGCA_C

Cuts at:      0    3239    3837 
   Size:       3239     598
 
ApoI r'AATT_y

Cuts at:      0     266     369     897     993    1566    1595    2507    2925 
   Size:        266     103     528      96     573      29     912     418

Cuts at:   2925    3373    3738    3837 
   Size:        448     365      99

  Fragments arranged by size: 

                 912     573     528     448     418     365     266     103
                  99      96      29
 
AvaI C'yCGr_G

Cuts at:      0    1478    3837 
   Size:       1478    2359
 
BamHI G'GATC_C

Cuts at:      0      39    3837 
   Size:         39    3798
 
BanII G_rGCy'C

Cuts at:      0    1335    2234    3837 
   Size:       1335     899    1603

  Fragments arranged by size: 

                1603    1335     899
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1855    1981    2569    3561    3647    3837 
   Size:       1855     126     588     992      86     190

  Fragments arranged by size: 

                1855     992     588     190     126      86
 
BbvCI CC'TCA_GC

Cuts at:      0    3080    3837 
   Size:       3080     757
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1127    1161    3837 
   Size:       1127      34    2676

  Fragments arranged by size: 

                2676    1127      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3466    3837 
   Size:       3466     371
 
BfrBI ATG'CAT

Cuts at:      0     175    3837 
   Size:        175    3662
 
BglII A'GATC_T

Cuts at:      0    2466    2472    3837 
   Size:       2466       6    1365

  Fragments arranged by size: 

                2466    1365       6
 
BlpI GC'TnA_GC

Cuts at:      0    2555    3837 
   Size:       2555    1282
 
Bme1580I G_kGCm'C

Cuts at:      0    3243    3525    3837 
   Size:       3243     282     312

  Fragments arranged by size: 

                3243     312     282
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1450    3230    3837 
   Size:       1450    1780     607

  Fragments arranged by size: 

                1780    1450     607
 
BmtI G_CTAG'C

Cuts at:      0    1207    3837 
   Size:       1207    2630
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2853    3347    3837 
   Size:       2853     494     490

  Fragments arranged by size: 

                2853     494     490
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1349    1842    2510    3073    3530    3837 
   Size:       1349     493     668     563     457     307

  Fragments arranged by size: 

                1349     668     563     493     457     307
 
Bpu10I CC'TnA_GC

Cuts at:      0    3080    3837 
   Size:       3080     757
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1043    3425    3837 
   Size:       1043    2382     412

  Fragments arranged by size: 

                2382    1043     412
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2697    3318    3837 
   Size:       2697     621     519

  Fragments arranged by size: 

                2697     621     519
 
BsaAI yAC'GTr

Cuts at:      0    2519    3837 
   Size:       2519    1318
 
BsaBI GATnn'nnATC

Cuts at:      0     338    2471    3837 
   Size:        338    2133    1366

  Fragments arranged by size: 

                2133    1366     338
 
BsaHI Gr'CG_yC

Cuts at:      0    1904    3837 
   Size:       1904    1933
 
BsaWI w'CCGG_w

Cuts at:      0      20    3837 
   Size:         20    3817
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2325    2355    2508    2538    3483    3513    3837 
   Size:       2325      30     153      30     945      30     324

  Fragments arranged by size: 

                2325     945     324     153      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     569    3320    3491    3837 
   Size:        569    2751     171     346

  Fragments arranged by size: 

                2751     569     346     171
 
BseYI C'CCAG_C

Cuts at:      0     634    3124    3731    3837 
   Size:        634    2490     607     106

  Fragments arranged by size: 

                2490     634     607     106
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1198    2524    3341    3837 
   Size:       1198    1326     817     496

  Fragments arranged by size: 

                1326    1198     817     496
 
BsiEI CG_ry'CG

Cuts at:      0    3293    3837 
   Size:       3293     544
 
BsiHKAI G_wGCw'C

Cuts at:      0     122    1335    1368    1485    2234    3213    3243    3384 
   Size:        122    1213      33     117     749     979      30     141

Cuts at:   3384    3837 
   Size:        453

  Fragments arranged by size: 

                1213     979     749     453     141     122     117      33
                  30
 
BsmI GAATG_Cn'

Cuts at:      0    1791    2030    3837 
   Size:       1791     239    1807

  Fragments arranged by size: 

                1807    1791     239
 
Bsp1286I G_dGCh'C

Cuts at:      0     122    1335    1368    1485    2234    3213    3243    3384 
   Size:        122    1213      33     117     749     979      30     141

Cuts at:   3384    3525    3837 
   Size:        141     312

  Fragments arranged by size: 

                1213     979     749     312     141     141     122     117
                  33      30
 
BspEI T'CCGG_A

Cuts at:      0      20    3837 
   Size:         20    3817
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     846    3474    3764    3837 
   Size:        846    2628     290      73

  Fragments arranged by size: 

                2628     846     290      73
 
BsrDI GCAATG_nn'

Cuts at:      0    2960    2965    3837 
   Size:       2960       5     872

  Fragments arranged by size: 

                2960     872       5
 
BsrGI T'GTAC_A

Cuts at:      0    2513    3837 
   Size:       2513    1324
 
BssSI C'ACGA_G

Cuts at:      0    3503    3837 
   Size:       3503     334
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     397    3013    3451    3837 
   Size:        397    2616     438     386

  Fragments arranged by size: 

                2616     438     397     386
 
BstBI TT'CG_AA

Cuts at:      0    1137    3837 
   Size:       1137    2700
 
BstEII G'GTnAC_C

Cuts at:      0    2331    3837 
   Size:       2331    1506
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     168     767    3837 
   Size:        168     599    3070

  Fragments arranged by size: 

                3070     599     168
 
BstYI r'GATC_y

Cuts at:      0      23      39     751     793     907    2466    2472    3837 
   Size:         23      16     712      42     114    1559       6    1365

  Fragments arranged by size: 

                1559    1365     712     114      42      23      16       6
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    3632    3837 
   Size:       3632     205
 
BtsI GCAGTG_nn'

Cuts at:      0     313    3837 
   Size:        313    3524
 
ClaI AT'CG_AT

Cuts at:      0     323    2204    3837 
   Size:        323    1881    1633

  Fragments arranged by size: 

                1881    1633     323
 
DraI TTT'AAA

Cuts at:      0    2903    3837 
   Size:       2903     934
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1527    3837 
   Size:       1527    2310
 
EarI CTCTTCn'nnn_

Cuts at:      0    2035    2544    2621    3135    3837 
   Size:       2035     509      77     514     702

  Fragments arranged by size: 

                2035     702     514     509      77
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3323    3837 
   Size:       3323     514
 
EcoICRI GAG'CTC

Cuts at:      0    1333    2232    3837 
   Size:       1333     899    1605

  Fragments arranged by size: 

                1605    1333     899
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     314    1349    1828    1842    2483    2510    2581 
   Size:        299      15    1035     479      14     641      27      71

Cuts at:   2581    2645    3073    3080    3323    3530    3646    3779    3837 
   Size:         64     428       7     243     207     116     133      58

  Fragments arranged by size: 

                1035     641     479     428     299     243     207     133
                 116      71      64      58      27      15      14       7
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3443    3837 
   Size:       3443     394
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3175    3463    3837 
   Size:       3175     288     374

  Fragments arranged by size: 

                3175     374     288
 
EcoRI G'AATT_C

Cuts at:      0     369    3837 
   Size:        369    3468
 
EcoRV GAT'ATC

Cuts at:      0     981    3837 
   Size:        981    2856
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     956    2352    2376    2803    3466    3709    3837 
   Size:        956    1396      24     427     663     243     128

  Fragments arranged by size: 

                1396     956     663     427     243     128      24
 
FspI TGC'GCA

Cuts at:      0     434    3837 
   Size:        434    3403
 
FspAI rTGC'GCAy

Cuts at:      0     434    3837 
   Size:        434    3403
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       8      40     295     327     548     580    1592    1624 
   Size:          8      32     255      32     221      32    1012      32

Cuts at:   1624    3483    3486    3515    3518    3814    3837 
   Size:       1859       3      29       3     296      23

  Fragments arranged by size: 

                1859    1012     296     255     221      32      32      32
                  32      29      23       8       3       3
 
HindIII A'AGCT_T

Cuts at:      0     938     959    3800    3837 
   Size:        938      21    2841      37

  Fragments arranged by size: 

                2841     938      37      21
 
MfeI C'AATT_G

Cuts at:      0    1757    3837 
   Size:       1757    2080
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     240    1196    1590    2368    3837 
   Size:        240     956     394     778    1469

  Fragments arranged by size: 

                1469     956     778     394     240
 
MslI CAynn'nnrTG

Cuts at:      0    2168    3837 
   Size:       2168    1669
 
MspA1I CmG'CkG

Cuts at:      0    2461    3837 
   Size:       2461    1376
 
NdeI CA'TA_TG

Cuts at:      0     177     926    3837 
   Size:        177     749    2911

  Fragments arranged by size: 

                2911     749     177
 
NheI G'CTAG_C

Cuts at:      0    1203    3837 
   Size:       1203    2634
 
NsiI A_TGCA'T

Cuts at:      0     177    3837 
   Size:        177    3660
 
NspI r_CATG'y

Cuts at:      0     447     973    1560    1939    3837 
   Size:        447     526     587     379    1898

  Fragments arranged by size: 

                1898     587     526     447     379
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2677    3837 
   Size:       2677    1160
 
PfoI T'CCnGG_A

Cuts at:      0    1712    3837 
   Size:       1712    2125
 
PmlI CAC'GTG

Cuts at:      0    2519    3837 
   Size:       2519    1318
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     740     772    1317    1349    3837 
   Size:        740      32     545      32    2488

  Fragments arranged by size: 

                2488     740     545      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    3175    3463    3837 
   Size:       3175     288     374

  Fragments arranged by size: 

                3175     374     288
 
PsiI TTA'TAA

Cuts at:      0    2645    2791    3837 
   Size:       2645     146    1046

  Fragments arranged by size: 

                2645    1046     146
 
PspXI vC'TCGA_Gb

Cuts at:      0    1478    3837 
   Size:       1478    2359
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2645    2677    3837 
   Size:       2645      32    1160

  Fragments arranged by size: 

                2645    1160      32
 
PstI C_TGCA'G

Cuts at:      0     132    2076    2759    3837 
   Size:        132    1944     683    1078

  Fragments arranged by size: 

                1944    1078     683     132
 
PvuII CAG'CTG

Cuts at:      0    2461    3837 
   Size:       2461    1376
 
SacI G_AGCT'C

Cuts at:      0    1335    2234    3837 
   Size:       1335     899    1603

  Fragments arranged by size: 

                1603    1335     899
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2035    2544    3135    3837 
   Size:       2035     509     591     702

  Fragments arranged by size: 

                2035     702     591     509
 
ScaI AGT'ACT

Cuts at:      0     167    1547    3837 
   Size:        167    1380    2290

  Fragments arranged by size: 

                2290    1380     167
 
SexAI A'CCwGG_T

Cuts at:      0     640    3837 
   Size:        640    3197
 
SfcI C'TryA_G

Cuts at:      0     128    1835    2058    2072    2755    3051    3837 
   Size:        128    1707     223      14     683     296     786

  Fragments arranged by size: 

                1707     786     683     296     223     128      14
 
SmlI C'TyrA_G

Cuts at:      0     865    1058    1478    3440    3837 
   Size:        865     193     420    1962     397

  Fragments arranged by size: 

                1962     865     420     397     193
 
SpeI A'CTAG_T

Cuts at:      0    3142    3837 
   Size:       3142     695
 
SphI G_CATG'C

Cuts at:      0     447    1939    3837 
   Size:        447    1492    1898

  Fragments arranged by size: 

                1898    1492     447
 
SspI AAT'ATT

Cuts at:      0    1579    3837 
   Size:       1579    2258
 
StuI AGG'CCT

Cuts at:      0    2580    3837 
   Size:       2580    1257
 
StyI C'CwwG_G

Cuts at:      0    3111    3837 
   Size:       3111     726
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1265    1710    3375    3399    3504    3837 
   Size:       1265     445    1665      24     105     333

  Fragments arranged by size: 

                1665    1265     445     333     105      24
 
TatI w'GTAC_w

Cuts at:      0     165     710    1545    2513    3478    3837 
   Size:        165     545     835     968     965     359

  Fragments arranged by size: 

                 968     965     835     545     359     165
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1784    1816    2403    2435    2508    2540    3837 
   Size:       1784      32     587      32      73      32    1297

  Fragments arranged by size: 

                1784    1297     587      73      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2419    3425    3837 
   Size:       2419    1006     412

  Fragments arranged by size: 

                2419    1006     412
 
XbaI T'CTAG_A

Cuts at:      0    1074    2469    3837 
   Size:       1074    1395    1368

  Fragments arranged by size: 

                1395    1368    1074
 
XhoI C'TCGA_G

Cuts at:      0    1478    3837 
   Size:       1478    2359
 
XmnI GAAnn'nnTTC

Cuts at:      0     324    1271    1396    3074    3837 
   Size:        324     947     125    1678     763

  Fragments arranged by size: 

                1678     947     763     324     125
 

 Enzymes that do cut:

     AarI     AcuI    AflII   AflIII     AlfI     AloI    AlwNI    ApaLI
     ApoI     AvaI    BamHI    BanII     BbsI    BbvCI     BcgI    BciVI
    BfrBI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI   Bsu36I     BtsI     ClaI     DraI     DrdI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    FspAI    Hin4I  HindIII     MfeI     MmeI     MslI   MspA1I     NdeI
     NheI     NsiI     NspI    PflMI     PfoI     PmlI     PpiI    PpuMI
     PsiI    PspXI     PsrI     PstI    PvuII     SacI     SapI     ScaI
    SexAI     SfcI     SmlI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BanI     BbeI
     BclI     BglI    BmgBI    BsiWI    BsmBI    BspHI    BsrBI    BsrFI
   BssHII  BstZ17I     BtgI    BtgZI    CspCI   DraIII     EaeI     EagI
     FseI    HaeII   HincII     HpaI     KasI     KpnI     MluI     MscI
     NaeI     NarI     NcoI   NgoMIV     NotI     NruI     PacI     PasI
     PciI     PmeI    PshAI   PspOMI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SrfI     SwaI     XcmI     XmaI     ZraI
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