Restriction map for pF1KA0901
 (Circular) (Six-base) MAPSORT of: pF1KA0901.seq  Check: 8384  from: 1  to: 6758



pF1KA0901  6758 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1220    2408    1220 
   Size:       1188    5570
 
AccI GT'mk_AC

Cuts at:   3560    3796    3560 
   Size:        236    6522
 
Acc65I G'GTAC_C

Cuts at:   1614    3774    1614 
   Size:       2160    4598
 
AclI AA'CG_TT

Cuts at:   6598    6598 
   Size:       6758
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    368     988    1304    1358    4583    5015    5762    6030     368 
   Size:        620     316      54    3225     432     747     268    1096

  Fragments arranged by size: 

                3225    1096     747     620     432     316     268      54
            
 
AflIII A'CryG_T

Cuts at:   2081    3750    4110    5214    6191    2081 
   Size:       1669     360    1104     977    2648

  Fragments arranged by size: 

                2648    1669    1104     977     360
 
AgeI A'CCGG_T

Cuts at:   5146    5146 
   Size:       6758
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    587     619     634     666     587 
   Size:         32      15      32    6679

  Fragments arranged by size: 

                6679      32      32      15
 
AlwNI CAG_nnn'CTG

Cuts at:    676    1708    2597    4063    5630     676 
   Size:       1032     889    1466    1567    1804

  Fragments arranged by size: 

                1804    1567    1466    1032     889
 
ApaI G_GGCC'C

Cuts at:   2231    2556    2231 
   Size:        325    6433
 
ApaLI G'TGCA_C

Cuts at:    868    1841    5528     868 
   Size:        973    3687    2098

  Fragments arranged by size: 

                3687    2098     973
 
ApoI r'AATT_y

Cuts at:    394    3606    3762    6111    6367     394 
   Size:       3212     156    2349     256     785

  Fragments arranged by size: 

                3212    2349     785     256     156
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6758
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6758
 
AvaI C'yCGr_G

Cuts at:   1102    1775    3180    3778    1102 
   Size:        673    1405     598    4082

  Fragments arranged by size: 

                4082    1405     673     598
 
AvrII C'CTAG_G

Cuts at:   2689    2689 
   Size:       6758
 
BamHI G'GATC_C

Cuts at:   2033    2346    3783    2033 
   Size:        313    1437    5008

  Fragments arranged by size: 

                5008    1437     313
 
BanI G'GyrC_C

Cuts at:   1348    1614    2137    2164    2670    3151    3774    4188    4436 
   Size:        266     523      27     506     481     623     414     248

Cuts at:   4436    4471    1348 
   Size:         35    3635

  Fragments arranged by size: 

                3635     623     523     506     481     414     266     248
                  35      27
 
BanII G_rGCy'C

Cuts at:   1406    1505    2231    2556    3772    4802    1406 
   Size:         99     726     325    1216    1030    3362

  Fragments arranged by size: 

                3362    1216    1030     726     325      99
 
BbeI G_GCGC'C

Cuts at:   4192    4440    4192 
   Size:        248    6510
 
BbsI GAAGACnn'nnnn_

Cuts at:    308    2633     308 
   Size:       2325    4433
 
BbvCI CC'TCA_GC

Cuts at:   1115    3628    1115 
   Size:       2513    4245
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2160    2194    3363    3397    2160 
   Size:         34    1169      34    5521

  Fragments arranged by size: 

                5521    1169      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4656    5417    6758    4656 
   Size:        761    1341    4656

  Fragments arranged by size: 

                4656    1341     761
 
BclI T'GATC_A

Cuts at:   2130    3405    4278    2130 
   Size:       1275     873    4610

  Fragments arranged by size: 

                4610    1275     873
 
BglI GCCn_nnn'nGGC

Cuts at:    664    1186    1705    2180    2866     664 
   Size:        522     519     475     686    4556

  Fragments arranged by size: 

                4556     686     522     519     475
 
BglII A'GATC_T

Cuts at:   4273    6310    4273 
   Size:       2037    4721
 
BlpI GC'TnA_GC

Cuts at:   1709    2699    3870    1709 
   Size:        990    1171    4597

  Fragments arranged by size: 

                4597    1171     990
 
Bme1580I G_kGCm'C

Cuts at:    872    1353    1368    1845    2197    2231    2556    3154    4383 
   Size:        481      15     477     352      34     325     598    1229

Cuts at:   4383    4476    5532     872 
   Size:         93    1056    2098

  Fragments arranged by size: 

                2098    1229    1056     598     481     477     352     325
                  93      34      15
 
BmgBI CAC'GTC

Cuts at:   1083    3492    1083 
   Size:       2409    4349
 
BmrI ACTGGGnnnn_n'

Cuts at:    162     570     905    1820    2093    3239    3970    4051    4386 
   Size:        408     335     915     273    1146     731      81     335

Cuts at:   4386    6530     162 
   Size:       2144     390

  Fragments arranged by size: 

                2144    1146     915     731     408     390     335     335
                 273      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5125    5125 
   Size:       6758
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    171    1273    1357    1669    2628    2768    3128    3371     171 
   Size:       1102      84     312     959     140     360     243    3558

  Fragments arranged by size: 

                3558    1102     959     360     312     243     140      84
            
 
Bpu10I CC'TnA_GC

Cuts at:    434    1115    3628     434 
   Size:        681    2513    3564

  Fragments arranged by size: 

                3564    2513     681
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    361    1420    3934    4813    5305    5603     361 
   Size:       1059    2514     879     492     298    1516

  Fragments arranged by size: 

                2514    1516    1059     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1313    1429    2322    2501    2566    6462    1313 
   Size:        116     893     179      65    3896    1609

  Fragments arranged by size: 

                3896    1609     893     179     116      65
 
BsaAI yAC'GTr

Cuts at:    798    1740    4111     798 
   Size:        942    2371    3445

  Fragments arranged by size: 

                3445    2371     942
 
BsaBI GATnn'nnATC

Cuts at:      6    4272    6309       6 
   Size:       4266    2037     455

  Fragments arranged by size: 

                4266    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1289    4189    4437    5139    6635    1289 
   Size:       2900     248     702    1496    1412

  Fragments arranged by size: 

                2900    1496    1412     702     248
 
BsaWI w'CCGG_w

Cuts at:    716     839    2949    3947    4468    5146    5420    5567    5978 
   Size:        123    2110     998     521     678     274     147     411

Cuts at:   5978     716 
   Size:       1496

  Fragments arranged by size: 

                2110    1496     998     678     521     411     274     147
                 123
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    634     664    1792    1822     634 
   Size:         30    1128      30    5570

  Fragments arranged by size: 

                5570    1128      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1474    1489    1498    1666    2941    2983    2996    3067    3080 
   Size:         15       9     168    1275      42      13      71      13

Cuts at:   3080    3151    3161    3340    1474 
   Size:         71      10     179    4892

  Fragments arranged by size: 

                4892    1275     179     168      71      71      42      15
                  13      13      10       9
 
BseYI C'CCAG_C

Cuts at:    758    1199    1619    1656    2352    2907    3033    4183    4941 
   Size:        441     420      37     696     555     126    1150     758

Cuts at:   4941    5518    6014     758 
   Size:        577     496    1502

  Fragments arranged by size: 

                1502    1150     758     696     577     555     496     441
                 420     126      37
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1063    1768    2104    2321    2472    3238    1063 
   Size:        705     336     217     151     766    4583

  Fragments arranged by size: 

                4583     766     705     336     217     151
 
BsiEI CG_ry'CG

Cuts at:    104    2184    4300    4346    5554     104 
   Size:       2080    2116      46    1208    1308

  Fragments arranged by size: 

                2116    2080    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    872    1389    1514    1845    1889    3187    3772    4550    4740 
   Size:        517     125     331      44    1298     585     778     190

Cuts at:   4740    5532     872 
   Size:        792    2098

  Fragments arranged by size: 

                2098    1298     792     778     585     517     331     190
                 125      44
 
Bsp1286I G_dGCh'C

Cuts at:    872    1353    1368    1389    1406    1505    1514    1845    1889 
   Size:        481      15      21      17      99       9     331      44

Cuts at:   1889    2197    2231    2556    3154    3187    3772    4383    4476 
   Size:        308      34     325     598      33     585     611      93

Cuts at:   4476    4550    4740    4802    5532     872 
   Size:         74     190      62     730    2098

  Fragments arranged by size: 

                2098     730     611     598     585     481     331     325
                 308     190      99      93      74      62      44      34
                  33      21      17      15       9
 
BspEI T'CCGG_A

Cuts at:    716     839    2949    5978     716 
   Size:        123    2110    3029    1496

  Fragments arranged by size: 

                3029    2110    1496     123
 
BspHI T'CATG_A

Cuts at:   2615    5934    2615 
   Size:       3319    3439
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1220    2408    3483    3808    4022    4324    4705    1220 
   Size:       1188    1075     325     214     302     381    3273

  Fragments arranged by size: 

                3273    1188    1075     381     325     302     214
 
BsrBI CCG'CTC

Cuts at:     50     233     438    2703    5050    5104    6588    6753      50 
   Size:        183     205    2265    2347      54    1484     165      55

  Fragments arranged by size: 

                2347    2265    1484     205     183     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:    299     399    1064    2282    4670     299 
   Size:        100     665    1218    2388    2387

  Fragments arranged by size: 

                2388    2387    1218     665     100
 
BsrFI r'CCGG_y

Cuts at:    118    4756    4937    5146     118 
   Size:       4638     181     209    1730

  Fragments arranged by size: 

                4638    1730     209     181
 
BssSI C'ACGA_G

Cuts at:   2714    5029    5387    2714 
   Size:       2315     358    4085

  Fragments arranged by size: 

                4085    2315     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1086    1849    4135    1086 
   Size:        763    2286    3709

  Fragments arranged by size: 

                3709    2286     763
 
BstBI TT'CG_AA

Cuts at:    199    5119     199 
   Size:       4920    1838
 
BstEII G'GTnAC_C

Cuts at:   2658    2658 
   Size:       6758
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2068    3440    3505    3601    2068 
   Size:       1372      65      96    5225

  Fragments arranged by size: 

                5225    1372      96      65
 
BstYI r'GATC_y

Cuts at:      1     330     713    2033    2346    3388    3783    4250    4273 
   Size:        329     383    1320     313    1042     395     467      23

Cuts at:   4273    4608    4854    5866    5952    5964    6310       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1320    1042    1012     467     449     395     383     346
                 335     329     313     246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    179     669     682    1610    2375     179 
   Size:        490      13     928     765    4562

  Fragments arranged by size: 

                4562     928     765     490      13
 
BtgI C'CryG_G

Cuts at:    873    1439    2061    2150    2477    2533    3101    3600     873 
   Size:        566     622      89     327      56     568     499    4031

  Fragments arranged by size: 

                4031     622     568     566     499     327      89      56
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2251    2572    3693    4708    4889    5052    2251 
   Size:        321    1121    1015     181     163    3957

  Fragments arranged by size: 

                3957    1121    1015     321     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1581    3433    4001    1581 
   Size:       1852     568    4338

  Fragments arranged by size: 

                4338    1852     568
 
DraI TTT'AAA

Cuts at:   3757    3757 
   Size:       6758
 
DraIII CAC_nnn'GTG

Cuts at:   1348    1348 
   Size:       6758
 
DrdI GACnn_nn'nnGTC

Cuts at:    190     913    4464    5322     190 
   Size:        723    3551     858    1626

  Fragments arranged by size: 

                3551    1626     858     723
 
EaeI y'GGCC_r

Cuts at:    120     285     728     999    1175    1528    2181    2421    3582 
   Size:        165     443     271     176     353     653     240    1161

Cuts at:   3582    4343    4517    4908    4935    6280     120 
   Size:        761     174     391      27    1345     598

  Fragments arranged by size: 

                1345    1161     761     653     598     443     391     353
                 271     240     176     174     165      27
 
EagI C'GGCC_G

Cuts at:   2181    4343    2181 
   Size:       2162    4596
 
EarI CTCTTCn'nnn_

Cuts at:    518    2768    4781    4991     518 
   Size:       2250    2013     210    2285

  Fragments arranged by size: 

                2285    2250    2013     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4966    5276    5422    6568    4966 
   Size:        310     146    1146    5156

  Fragments arranged by size: 

                5156    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   3770    3770 
   Size:       6758
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    171     368     988    1273    1304    1357    1358    1669    2628 
   Size:        197     620     285      31      53       1     311     959

Cuts at:   2628    2768    3128    3371    4583    5015    5762    6030     171 
   Size:        140     360     243    1212     432     747     268     899

  Fragments arranged by size: 

                1212     959     899     747     620     432     360     311
                 285     268     243     197     140      53      31       1
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1876    2824    3479    1876 
   Size:        948     655    5155

  Fragments arranged by size: 

                5155     948     655
 
EcoO109I rG'GnC_Cy

Cuts at:    430     474     846     906    1158    1492    2228    2552    2553 
   Size:         44     372      60     252     334     736     324       1

Cuts at:   2553    2638    3269    3299    3315    3396    3897     430 
   Size:         85     631      30      16      81     501    3291

  Fragments arranged by size: 

                3291     736     631     501     372     334     324     252
                  85      81      60      44      30      16       1
 
EcoRI G'AATT_C

Cuts at:    394    3762     394 
   Size:       3368    3390
 
EcoRV GAT'ATC

Cuts at:   3239    3239 
   Size:       6758
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4777    4777 
   Size:       6758
 
FspI TGC'GCA

Cuts at:   4539    4539 
   Size:       6758
 
HaeII r_GCGC'y

Cuts at:   1674    1862    4192    4440    5462    6441    1674 
   Size:        188    2330     248    1022     979    1991

  Fragments arranged by size: 

                2330    1991    1022     979     248     188
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1004    1036    1233    1265    2821    2853    3153    3185    3246 
   Size:         32     197      32    1556      32     300      32      61

Cuts at:   3246    3278    4600    4630    4632    4662    1004 
   Size:         32    1322      30       2      30    3100

  Fragments arranged by size: 

                3100    1556    1322     300     197      61      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   2211    3797    2211 
   Size:       1586    5172
 
KasI G'GCGC_C

Cuts at:   4188    4436    4188 
   Size:        248    6510
 
KpnI G_GTAC'C

Cuts at:   1618    3778    1618 
   Size:       2160    4598
 
MluI A'CGCG_T

Cuts at:   6191    6191 
   Size:       6758
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    356    1823    3618    5429    5613     356 
   Size:       1467    1795    1811     184    1501

  Fragments arranged by size: 

                1811    1795    1501    1467     184
 
MscI TGG'CCA

Cuts at:    730    1530    3584    4519     730 
   Size:        800    2054     935    2969

  Fragments arranged by size: 

                2969    2054     935     800
 
MslI CAynn'nnrTG

Cuts at:   1024    2066    2126    4747    4874    1024 
   Size:       1042      60    2621     127    2908

  Fragments arranged by size: 

                2908    2621    1042     127      60
 
MspA1I CmG'CkG

Cuts at:   1119    2323    2352    2809    3349    3621    3869    4183    4543 
   Size:       1204      29     457     540     272     248     314     360

Cuts at:   4543    5556    5801    1119 
   Size:       1013     245    2076

  Fragments arranged by size: 

                2076    1204    1013     540     457     360     314     272
                 248     245      29
 
NaeI GCC'GGC

Cuts at:   4939    4939 
   Size:       6758
 
NarI GG'CG_CC

Cuts at:   4189    4437    4189 
   Size:        248    6510
 
NcoI C'CATG_G

Cuts at:   2150    2477    3101    3600    2150 
   Size:        327     624     499    5308

  Fragments arranged by size: 

                5308     624     499     327
 
NgoMIV G'CCGG_C

Cuts at:   4937    4937 
   Size:       6758
 
NspI r_CATG'y

Cuts at:    789    2026    2085    3591    3811    4311    5218     789 
   Size:       1237      59    1506     220     500     907    2329

  Fragments arranged by size: 

                2329    1506    1237     907     500     220      59
 
PasI CC'CwG_GG

Cuts at:   1496    1496 
   Size:       6758
 
PciI A'CATG_T

Cuts at:   2081    5214    2081 
   Size:       3133    3625
 
PflMI CCAn_nnn'nTGG

Cuts at:    294    3456     294 
   Size:       3162    3596
 
PfoI T'CCnGG_A

Cuts at:    422     467     744     422 
   Size:         45     277    6436

  Fragments arranged by size: 

                6436     277      45
 
PmeI GTTT'AAAC

Cuts at:   3757    3757 
   Size:       6758
 
PmlI CAC'GTG

Cuts at:    798     798 
   Size:       6758
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    634     666    5897    5929     634 
   Size:         32    5231      32    1463

  Fragments arranged by size: 

                5231    1463      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    846     906    1158    1492    2638    3269     846 
   Size:         60     252     334    1146     631    4335

  Fragments arranged by size: 

                4335    1146     631     334     252      60
 
PspOMI G'GGCC_C

Cuts at:   2227    2552    2227 
   Size:        325    6433
 
PspXI vC'TCGA_Gb

Cuts at:   1102    3180    1102 
   Size:       2078    4680
 
PstI C_TGCA'G

Cuts at:   3480    3805    4490    3480 
   Size:        325     685    5748

  Fragments arranged by size: 

                5748     685     325
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6758
 
PvuII CAG'CTG

Cuts at:   1119    2323    2809    3349    4183    4543    1119 
   Size:       1204     486     540     834     360    3334

  Fragments arranged by size: 

                3334    1204     834     540     486     360
 
RsrII CG'GwC_CG

Cuts at:   4953    4953 
   Size:       6758
 
SacI G_AGCT'C

Cuts at:   3772    3772 
   Size:       6758
 
SalI G'TCGA_C

Cuts at:   3795    3795 
   Size:       6758
 
SanDI GG'GwC_CC

Cuts at:   1158    1158 
   Size:       6758
 
SapI GCTCTTCn'nnn_

Cuts at:    518    4781    4991     518 
   Size:       4263     210    2285

  Fragments arranged by size: 

                4263    2285     210
 
SbfI CC_TGCA'GG

Cuts at:   3805    3805 
   Size:       6758
 
ScaI AGT'ACT

Cuts at:   6275    6275 
   Size:       6758
 
SexAI A'CCwGG_T

Cuts at:   1037    1344    1037 
   Size:        307    6451
 
SfcI C'TryA_G

Cuts at:     33    1537    3059    3476    3801    4486    5479    5670      33 
   Size:       1504    1522     417     325     685     993     191    1121

  Fragments arranged by size: 

                1522    1504    1121     993     685     417     325     191
            
 
SfoI GGC'GCC

Cuts at:   4190    4438    4190 
   Size:        248    6510
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6758
 
SmaI CCC'GGG

Cuts at:   1777    3780    1777 
   Size:       2003    4755
 
SmlI C'TyrA_G

Cuts at:    340    1102    1399    3180    3913    4828    5320    5582     340 
   Size:        762     297    1781     733     915     492     262    1516

  Fragments arranged by size: 

                1781    1516     915     762     733     492     297     262
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6758
 
SphI G_CATG'C

Cuts at:   2026    3811    4311    2026 
   Size:       1785     500    4473

  Fragments arranged by size: 

                4473    1785     500
 
SspI AAT'ATT

Cuts at:    210     210 
   Size:       6758
 
StuI AGG'CCT

Cuts at:    741     973    3485    3670     741 
   Size:        232    2512     185    3829

  Fragments arranged by size: 

                3829    2512     232     185
 
StyI C'CwwG_G

Cuts at:   1002    1008    1030    1163    2150    2477    2502    2689    3101 
   Size:          6      22     133     987     327      25     187     412

Cuts at:   3101    3272    3600    3892    4246    1002 
   Size:        171     328     292     354    3514

  Fragments arranged by size: 

                3514     987     412     354     328     327     292     187
                 171     133      25      22       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2227    2410    3525    3761    4341    4474    4933    5142    2227 
   Size:        183    1115     236     580     133     459     209    3843

  Fragments arranged by size: 

                3843    1115     580     459     236     209     183     133
            
 
TatI w'GTAC_w

Cuts at:    578    3247    6273     578 
   Size:       2669    3026    1063

  Fragments arranged by size: 

                3026    2669    1063
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6275    6307    6275 
   Size:         32    6726
 
Tth111I GACn'n_nGTC

Cuts at:   3129    4555    3129 
   Size:       1426    5332
 
XbaI T'CTAG_A

Cuts at:   3789    3789 
   Size:       6758
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1170    1523    2014    2065    2652    3607    3724    4253    1170 
   Size:        353     491      51     587     955     117     529    3675

  Fragments arranged by size: 

                3675     955     587     529     491     353     117      51
            
 
XhoI C'TCGA_G

Cuts at:   1102    3180    1102 
   Size:       2078    4680
 
XmaI C'CCGG_G

Cuts at:   1775    3778    1775 
   Size:       2003    4755
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AscI     BaeI
    BfrBI     BmtI    BsiWI     BsmI    BsmBI    BsrGI   BssHII  BstZ17I
     ClaI    CspCI     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI    PshAI     PsiI     PsrI
    SacII     SfiI    SgrAI    SnaBI     SrfI     SwaI     XmnI     ZraI
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