Restriction map for pF1KA0829
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaLI       
AscI        Asp718I     AspEI       AspI        AvrII       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BcnI        BglI        BlnI        BlpI        Bpu1102I    
BsaWI       BseAI       BsiEI       BsiWI       BsmI        
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstZ17I     CelII       Cfr10I      CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       FauI        FseI        HapII       
HgaI        HpaII       KasI        KpnI        KspI        
MluI        MroI        MspI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SmaI        Sse8387I    SwaI        Tth111I     
XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII         407  GACGTC               GACGT^C
AciI         3625  GCGG                 CCGC(-3/-1)
AcyI          407  GACGTC               G[GA]^CG[CT]C
AocI          859  CCTAAGG              CC^T[ACGT]AGG
ApaI         1400  GGGCCC               GGGCC^C
AseI         3117  ATTAAT               AT^TAAT
AsnI         3117  ATTAAT               AT^TAAT
AvaI         2420  CTCGAG               C^[CT]CG[GA]G
AviII        2327  TGCGCA               TGC^GCA
BamHI        2194  GGATCC               G^GATCC
BsaBI         988  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         407  GACGTC               G[GA]^CG[CT]C
BsaI         1586  GAGACC               GGTCTC(1/5)
BsoBI        2420  CTCGAG               C^[CT]CG[GA]G
BspDI        1483  ATCGAT               AT^CGAT
BstEII       1910  GGTCACC              G^GT[ACGT]ACC
BstPI        1910  GGTCACC              G^GT[ACGT]ACC
Bsu36I        859  CCTAAGG              CC^T[ACGT]AGG
ClaI         1483  ATCGAT               AT^CGAT
DsaI         2902  CCACGG               C^C[GA][CT]GG
Eco81I        859  CCTAAGG              CC^T[ACGT]AGG
EcoO65I      1910  GGTCACC              G^GT[ACGT]ACC
FspI         2327  TGCGCA               TGC^GCA
HaeII           7  AGCGCC               [GA]GCGC^[CT]
Hin1I         407  GACGTC               G[GA]^CG[CT]C
HindIII      2588  AAGCTT               A^AGCTT
MamI          988  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          257  CAATTG               C^AATTG
MunI          257  CAATTG               C^AATTG
PaeR7I       2420  CTCGAG               C^TCGAG
PflMI        2277  CCAAGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        3280  GACAGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3117  ATTAAT               AT^TAAT
SapI         1312  GCTCTTC              GCTCTTC(1/4)
SexAI        1870  ACCAGGT              A^CC[AT]GGT
SnaBI        1504  TACGTA               TAC^GTA
SspI          895  AATATT               AAT^ATT
StuI         1933  AGGCCT               AGG^CCT
Van91I       2277  CCAAGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         2420  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AflII        2550  CTTAAG               C^TTAAG
             2908  CTTAAG              
AflIII       1367  ACATGT               A^C[GA][CT]GT
             3454  ACATGT              
BalI          597  TGGCCA               TGG^CCA
             2132  TGGCCA              
BclI          993  TGATCA               T^GATCA
             1743  TGATCA              
BfrI         2550  CTTAAG               C^TTAAG
             2908  CTTAAG              
BglII         636  AGATCT               A^GATCT
             1698  AGATCT              
BpmI          353  CTCCAG               CTGGAG(16/14)
             1565  CTCCAG              
BsaAI        1504  TACGTA               [CT]AC^GT[GA]
             3446  TACGTG              
BseRI        1526  CTCCTC               GAGGAG(10/8)
             2172  CTCCTC              
BsmFI        1779  GGGAC                GGGAC(10/14)
             2439  GGGAC               
BspLU11I     1367  ACATGT               A^CATGT
             3454  ACATGT              
BstXI          26  CCAATTTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              113  CCATCAAGTTGG        
CfoI            8  GCGC                 GCG^C
             2328  GCGC                
DraI         1147  TTTAAA               TTT^AAA
             3211  TTTAAA              
DraII        1399  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1400  GGGCCCT             
EaeI          597  TGGCCA               [CT]^GGCC[GA]
             2132  TGGCCA              
EcoO109I     1399  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1400  GGGCCCT             
EcoRI        1108  GAATTC               G^AATTC
             3589  GAATTC              
EcoRV        2922  GATATC               GAT^ATC
             3301  GATATC              
EcoT22I      2116  ATGCAT               ATGCA^T
             2633  ATGCAT              
FbaI          993  TGATCA               T^GATCA
             1743  TGATCA              
HhaI            8  GCGC                 GCG^C
             2328  GCGC                
HinP1I          8  GCGC                 G^CGC
             2328  GCGC                
HpaI         1371  GTTAAC               GTT^AAC
             1875  GTTAAC              
MluNI         597  TGGCCA               TGG^CCA
             2132  TGGCCA              
MscI          597  TGGCCA               TGG^CCA
             2132  TGGCCA              
NdeI         2780  CATATG               CA^TATG
             2936  CATATG              
NsiI         2116  ATGCAT               ATGCA^T
             2633  ATGCAT              
PleI         1287  GAGTC                GAGTC(4/5)
             3573  GAGTC               
PstI          100  CTGCAG               CTGCA^G
             3415  CTGCAG              
SacI         2086  GAGCTC               GAGCT^C
             2249  GAGCTC              
ScaI          818  AGTACT               AGT^ACT
             3411  AGTACT              
SpeI         2698  ACTAGT               A^CTAGT
             3685  ACTAGT              
SphI         2330  GCATGC               GCATG^C
             2425  GCATGC              
XbaI         2079  TCTAGA               T^CTAGA
             3276  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaII       BanII       
BbsI        BbvI        BciVI       BfaI        BmyI        
BpuAI       BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       Bsp1286I    Bsp1407I    
BsrDI       BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HincII      HindII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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