Restriction map for pF1KA0751
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AgeI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
Asp718I     AspI        AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BfrI        BglI        
BlnI        BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BspLU11I    BsrFI       
BssHII      Bst1107I    BstXI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
EcoT22I     FseI        FspI        HaeII       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PstI        PvuI        RsrII       SacI        SacII       
SalI        SbfI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SwaI        
Tth111I     XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI         2952  GTCTAC               GT^[AC][GT]AC
AccII        1610  CGCG                 CG^CG
AclI         2838  AACGTT               AA^CGTT
AcyI         3077  GACGCC               G[GA]^CG[CT]C
AflIII       1210  ACGTGT               A^C[GA][CT]GT
AhdI         2519  GACATCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        3082  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         3164  GGGCCC               GGGCC^C
Asp700I      3509  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2519  GACATCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1146  TGGCCA               TGG^CCA
BamHI         485  GGATCC               G^GATCC
BcgI         1087  GCAGTTTTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         210  GGATAC               GTATCC(6/5)
BclI          288  TGATCA               T^GATCA
BcnI         3162  CCGGG                CC^[GC]GG
BlpI         3064  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3064  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1209  TACGTG               [CT]AC^GT[GA]
BsaHI        3077  GACGCC               G[GA]^CG[CT]C
BsaWI        2485  ACCGGA               [AT]^CCGG[AT]
Bsp1407I     1535  TGTACA               T^GTACA
BspHI        2678  TCATGA               T^CATGA
BsrGI        1535  TGTACA               T^GTACA
BssSI        2027  CTCGTG               CACGAG(-5/-1)
BstAPI       3091  GCAACACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        3316  TTCGAA               TT^CGAA
BstUI        1610  CGCG                 CG^CG
CelII        3064  GCTCAGC              GC^T[ACGT]AGC
DraII        2470  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       2420  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2294  GACATGATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2519  GACATCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     2470  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI        2050  GAATTC               G^AATTC
FbaI          288  TGATCA               T^GATCA
Hin1I        3077  GACGCC               G[GA]^CG[CT]C
MluNI        1146  TGGCCA               TGG^CCA
MscI         1146  TGGCCA               TGG^CCA
MspA1I       3142  CAGCTG               C[AC]G^C[GT]G
MvnI         1610  CGCG                 CG^CG
NciI         3162  CCGGG                CC^[GC]GG
NspI         1629  ACATGC               [GA]CATG^[CT]
NspV         3316  TTCGAA               TT^CGAA
PpuMI        2470  GGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I     2838  AACGTT               AA^CGTT
PvuII        3142  CAGCTG               CAG^CTG
RcaI         2678  TCATGA               T^CATGA
SapI         3509  GAAGAGC              GCTCTTC(1/4)
ScaI         2782  AGTACT               AGT^ACT
SfaNI        2688  GATGC                GCATC(5/9)
SfuI         3316  TTCGAA               TT^CGAA
SpeI         1075  ACTAGT               A^CTAGT
SspBI        1535  TGTACA               T^GTACA
SspI          188  AATATT               AAT^ATT
XbaI         1548  TCTAGA               T^CTAGA
XmnI         3509  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AseI         1794  ATTAAT               AT^TAAT
             2697  ATTAAT              
AsnI         1794  ATTAAT               AT^TAAT
             2697  ATTAAT              
AspHI         506  GAGCAC               G[AT]GC[AT]^C
             2874  GAGCAC              
BanII        2196  GAGCCC               G[GA]GC[CT]^C
             3164  GGGCCC              
BsiHKAI       506  GAGCAC               G[AT]GC[AT]^C
             2874  GAGCAC              
BsmI         1997  GAATGC               GAATGC(1/-1)
              330  GCATTC              
BspMI        2727  ACCTGC               ACCTGC(4/8)
             3096  ACCTGC              
BsrBI        2643  CCGCTC               CCGCTC(-3/-3)
             2629  GAGCGG              
BstEII       1786  GGTCACC              G^GT[ACGT]ACC
             2417  GGTCACC             
BstPI        1786  GGTCACC              G^GT[ACGT]ACC
             2417  GGTCACC             
CfoI           18  GCGC                 GCG^C
              945  GCGC                
EcoNI        1227  CCTTAACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2732  CCTTATCGAGG         
EcoO65I      1786  GGTCACC              G^GT[ACGT]ACC
             2417  GGTCACC             
FauI         2474  CCCGC                CCCGC(4/6)
             2642  CCCGC               
HapII        2486  CCGG                 C^CGG
             3162  CCGG                
HhaI           18  GCGC                 GCG^C
              945  GCGC                
HinP1I         18  GCGC                 G^CGC
              945  GCGC                
HpaII        2486  CCGG                 C^CGG
             3162  CCGG                
MslI           46  CATTTTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              526  CATAGTGATG          
MspI         2486  CCGG                 C^CGG
             3162  CCGG                
NcoI          632  CCATGG               C^CATGG
             2187  CCATGG              
PaeR7I        668  CTCGAG               C^TCGAG
             1346  CTCGAG              
PflMI        1149  CCACTCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3081  CCAGACTCTGG         
PshBI        1794  ATTAAT               AT^TAAT
             2697  ATTAAT              
StuI         1591  AGGCCT               AGG^CCT
             2901  AGGCCT              
Van91I       1149  CCACTCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3081  CCAGACTCTGG         
XhoI          668  CTCGAG               C^TCGAG
             1346  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaI        AvaII       BbsI        BbvI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaBI       BsaI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DsaI        EaeI        EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HgaI        HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SexAI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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