Restriction map for pF1KA0751
 (Linear) (Six-base) MAPSORT of: KIAA0751.seq  Check: 9589  from: 1  to: 3564



KIAA0751.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2736    3105    3564 
   Size:       2736     369     459

  Fragments arranged by size: 

                2736     459     369
 
AccI GT'mk_AC

Cuts at:      0    2953    3564 
   Size:       2953     611
 
AclI AA'CG_TT

Cuts at:      0    2839    3564 
   Size:       2839     725
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    2104    3564 
   Size:        515    1589    1460

  Fragments arranged by size: 

                1589    1460     515
 
AflIII A'CryG_T

Cuts at:      0    1210    3564 
   Size:       1210    2354
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2524    3564 
   Size:       2524    1040
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3087    3564 
   Size:       3087     477
 
ApaI G_GGCC'C

Cuts at:      0    3168    3564 
   Size:       3168     396
 
ApoI r'AATT_y

Cuts at:      0     435    1868    1984    2050    3564 
   Size:        435    1433     116      66    1514

  Fragments arranged by size: 

                1514    1433     435     116      66
 
AseI AT'TA_AT

Cuts at:      0    1795    2698    3564 
   Size:       1795     903     866

  Fragments arranged by size: 

                1795     903     866
 
AvaI C'yCGr_G

Cuts at:      0     503     668    1346    3564 
   Size:        503     165     678    2218

  Fragments arranged by size: 

                2218     678     503     165
 
BamHI G'GATC_C

Cuts at:      0     485    3564 
   Size:        485    3079
 
BanII G_rGCy'C

Cuts at:      0    2200    3168    3564 
   Size:       2200     968     396

  Fragments arranged by size: 

                2200     968     396
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     287    1509    2184    3564 
   Size:        287    1222     675    1380

  Fragments arranged by size: 

                1380    1222     675     287
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1076    1110    3564 
   Size:       1076      34    2454

  Fragments arranged by size: 

                2454    1076      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     204    3564 
   Size:        204    3360
 
BclI T'GATC_A

Cuts at:      0     288    3564 
   Size:        288    3276
 
BglII A'GATC_T

Cuts at:      0     226     607    1821    2307    2710    3564 
   Size:        226     381    1214     486     403     854

  Fragments arranged by size: 

                1214     854     486     403     381     226
 
BlpI GC'TnA_GC

Cuts at:      0    3065    3564 
   Size:       3065     499
 
Bme1580I G_kGCm'C

Cuts at:      0    3168    3564 
   Size:       3168     396
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2417    2765    3564 
   Size:       2417     348     799

  Fragments arranged by size: 

                2417     799     348
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1660    1952    2531    2631    2771    3166    3564 
   Size:       1660     292     579     100     140     395     398

  Fragments arranged by size: 

                1660     579     398     395     292     140     100
 
Bpu10I CC'TnA_GC

Cuts at:      0     580     677    3564 
   Size:        580      97    2887

  Fragments arranged by size: 

                2887     580      97
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     786    2647    3564 
   Size:        786    1861     917

  Fragments arranged by size: 

                1861     917     786
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     891    2381    2747    3564 
   Size:        891    1490     366     817

  Fragments arranged by size: 

                1490     891     817     366
 
BsaAI yAC'GTr

Cuts at:      0    1211    3564 
   Size:       1211    2353
 
BsaBI GATnn'nnATC

Cuts at:      0     231     612    1289    2601    3564 
   Size:        231     381     677    1312     963

  Fragments arranged by size: 

                1312     963     677     381     231
 
BsaHI Gr'CG_yC

Cuts at:      0    3078    3564 
   Size:       3078     486
 
BsaWI w'CCGG_w

Cuts at:      0    2485    3564 
   Size:       2485    1079
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2606    2636    3480    3510    3564 
   Size:       2606      30     844      30      54

  Fragments arranged by size: 

                2606     844      54      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     750     985    1249    2369    2423    3488    3564 
   Size:        750     235     264    1120      54    1065      76

  Fragments arranged by size: 

                1120    1065     750     264     235      76      54
 
BsiHKAI G_wGCw'C

Cuts at:      0     510    2878    3564 
   Size:        510    2368     686

  Fragments arranged by size: 

                2368     686     510
 
BsmI GAATG_Cn'

Cuts at:      0     330    2003    3564 
   Size:        330    1673    1561

  Fragments arranged by size: 

                1673    1561     330
 
Bsp1286I G_dGCh'C

Cuts at:      0     510    2200    2878    3168    3564 
   Size:        510    1690     678     290     396

  Fragments arranged by size: 

                1690     678     510     396     290
 
BspHI T'CATG_A

Cuts at:      0    2678    3564 
   Size:       2678     886
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2736    3105    3564 
   Size:       2736     369     459

  Fragments arranged by size: 

                2736     459     369
 
BsrBI CCG'CTC

Cuts at:      0    2631    2645    3564 
   Size:       2631      14     919

  Fragments arranged by size: 

                2631     919      14
 
BsrDI GCAATG_nn'

Cuts at:      0      13     224    1367    2140    3107    3564 
   Size:         13     211    1143     773     967     457

  Fragments arranged by size: 

                1143     967     773     457     211      13
 
BsrGI T'GTAC_A

Cuts at:      0    1535    3564 
   Size:       1535    2029
 
BssSI C'ACGA_G

Cuts at:      0    2027    3564 
   Size:       2027    1537
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3097    3564 
   Size:       3097     467
 
BstBI TT'CG_AA

Cuts at:      0    3317    3564 
   Size:       3317     247
 
BstEII G'GTnAC_C

Cuts at:      0    1786    2417    3564 
   Size:       1786     631    1147

  Fragments arranged by size: 

                1786    1147     631
 
BstYI r'GATC_y

Cuts at:      0     226     485     607     804    1821    2307    2596    2650 
   Size:        226     259     122     197    1017     486     289      54

Cuts at:   2650    2710    3483    3564 
   Size:         60     773      81

  Fragments arranged by size: 

                1017     773     486     289     259     226     197     122
                  81      60      54
 
BtgI C'CryG_G

Cuts at:      0     632    2187    2909    3168    3564 
   Size:        632    1555     722     259     396

  Fragments arranged by size: 

                1555     722     632     396     259
 
BtsI GCAGTG_nn'

Cuts at:      0    3199    3564 
   Size:       3199     365
 
DraIII CAC_nnn'GTG

Cuts at:      0    2425    3564 
   Size:       2425    1139
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2300    3564 
   Size:       2300    1264
 
EaeI y'GGCC_r

Cuts at:      0    1146    2906    3369    3564 
   Size:       1146    1760     463     195

  Fragments arranged by size: 

                1760    1146     463     195
 
EarI CTCTTCn'nnn_

Cuts at:      0     398    1154    1974    2142    3314    3504    3564 
   Size:        398     756     820     168    1172     190      60

  Fragments arranged by size: 

                1172     820     756     398     190     168      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    1660    1952    2104    2531    2631    2771    3166 
   Size:        515    1145     292     152     427     100     140     395

Cuts at:   3166    3564 
   Size:        398

  Fragments arranged by size: 

                1145     515     427     398     395     292     152     140
                 100
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1231    2736    3564 
   Size:       1231    1505     828

  Fragments arranged by size: 

                1505    1231     828
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2471    3564 
   Size:       2471    1093
 
EcoRI G'AATT_C

Cuts at:      0    2050    3564 
   Size:       2050    1514
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1594    1874    3530    3564 
   Size:       1594     280    1656      34

  Fragments arranged by size: 

                1656    1594     280      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     238     270     397     429     580     612     796     828 
   Size:        238      32     127      32     151      32     184      32

Cuts at:    828    2510    2542    2730    2762    3370    3402    3564 
   Size:       1682      32     188      32     608      32     162

  Fragments arranged by size: 

                1682     608     238     188     184     162     151     127
                  32      32      32      32      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     335    1076    2425    2822    3511    3564 
   Size:        335     741    1349     397     689      53

  Fragments arranged by size: 

                1349     741     689     397     335      53
 
MscI TGG'CCA

Cuts at:      0    1148    3564 
   Size:       1148    2416
 
MslI CAynn'nnrTG

Cuts at:      0      50     530    3564 
   Size:         50     480    3034

  Fragments arranged by size: 

                3034     480      50
 
MspA1I CmG'CkG

Cuts at:      0    3144    3564 
   Size:       3144     420
 
NcoI C'CATG_G

Cuts at:      0     632    2187    3564 
   Size:        632    1555    1377

  Fragments arranged by size: 

                1555    1377     632
 
NspI r_CATG'y

Cuts at:      0    1633    3564 
   Size:       1633    1931
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1155    3087    3564 
   Size:       1155    1932     477

  Fragments arranged by size: 

                1932    1155     477
 
PfoI T'CCnGG_A

Cuts at:      0     565    1713    3044    3564 
   Size:        565    1148    1331     520

  Fragments arranged by size: 

                1331    1148     565     520
 
PpuMI rG'GwC_Cy

Cuts at:      0    2471    3564 
   Size:       2471    1093
 
PsiI TTA'TAA

Cuts at:      0    2495    3564 
   Size:       2495    1069
 
PspOMI G'GGCC_C

Cuts at:      0    3164    3564 
   Size:       3164     400
 
PvuII CAG'CTG

Cuts at:      0    3144    3564 
   Size:       3144     420
 
SanDI GG'GwC_CC

Cuts at:      0    2471    3564 
   Size:       2471    1093
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3504    3564 
   Size:       3504      60
 
ScaI AGT'ACT

Cuts at:      0    2784    3564 
   Size:       2784     780
 
SexAI A'CCwGG_T

Cuts at:      0    1108    1473    3186    3564 
   Size:       1108     365    1713     378

  Fragments arranged by size: 

                1713    1108     378     365
 
SfcI C'TryA_G

Cuts at:      0    1457    2954    2976    3564 
   Size:       1457    1497      22     588

  Fragments arranged by size: 

                1497    1457     588      22
 
SmlI C'TyrA_G

Cuts at:      0     668     765    1346    2626    3564 
   Size:        668      97     581    1280     938

  Fragments arranged by size: 

                1280     938     668     581      97
 
SpeI A'CTAG_T

Cuts at:      0    1075    3564 
   Size:       1075    2489
 
SspI AAT'ATT

Cuts at:      0     190    3564 
   Size:        190    3374
 
StuI AGG'CCT

Cuts at:      0    1593    2903    3564 
   Size:       1593    1310     661

  Fragments arranged by size: 

                1593    1310     661
 
StyI C'CwwG_G

Cuts at:      0     632    1849    2187    3054    3491    3564 
   Size:        632    1217     338     867     437      73

  Fragments arranged by size: 

                1217     867     632     437     338      73
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2436    3094    3564 
   Size:       2436     658     470

  Fragments arranged by size: 

                2436     658     470
 
TatI w'GTAC_w

Cuts at:      0    1136    1535    2782    2890    3564 
   Size:       1136     399    1247     108     674

  Fragments arranged by size: 

                1247    1136     674     399     108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2411    2443    2646    2678    3564 
   Size:       2411      32     203      32     886

  Fragments arranged by size: 

                2411     886     203      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1548    3564 
   Size:       1548    2016
 
XhoI C'TCGA_G

Cuts at:      0     668    1346    3564 
   Size:        668     678    2218

  Fragments arranged by size: 

                2218     678     668
 
XmnI GAAnn'nnTTC

Cuts at:      0    3513    3564 
   Size:       3513      51
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI   AflIII     AhdI    AlwNI     ApaI
     ApoI     AseI     AvaI    BamHI    BanII     BbsI     BcgI    BciVI
     BclI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI     BtsI   DraIII     DrdI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I
     MmeI     MscI     MslI   MspA1I     NcoI     NspI    PflMI     PfoI
    PpuMI     PsiI   PspOMI    PvuII    SanDI     SapI     ScaI    SexAI
     SfcI     SmlI     SpeI     SspI     StuI     StyI    TaqII     TatI
     TstI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII     AgeI     AleI     AlfI     AloI
    ApaLI     AscI    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI
    BfrBI     BglI    BmgBI     BmtI     BplI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BsrFI   BssHII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI     EagI     EciI  EcoICRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SphI     SrfI     SwaI  Tth111I     XcmI     XmaI     ZraI
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