Restriction map for pF1KA0580
 (Circular) (Six-base) MAPSORT of: pF1KA0580.seq  Check: 4633  from: 1  to: 8225



pF1KA0580  8225 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   4290    4290 
   Size:       8225
 
AccI GT'mk_AC

Cuts at:   2923    5263    2923 
   Size:       2340    5885
 
Acc65I G'GTAC_C

Cuts at:   5241    5241 
   Size:       8225
 
AclI AA'CG_TT

Cuts at:   4758    8065    4758 
   Size:       3307    4918
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2822    5051    6050    6482    7229    7497    2822 
   Size:       2229     999     432     747     268    3550

  Fragments arranged by size: 

                3550    2229     999     747     432     268
 
AflII C'TTAA_G

Cuts at:   3285    3285 
   Size:       8225
 
AflIII A'CryG_T

Cuts at:   3042    5577    6681    7658    3042 
   Size:       2535    1104     977    3609

  Fragments arranged by size: 

                3609    2535    1104     977
 
AgeI A'CCGG_T

Cuts at:   4481    6613    4481 
   Size:       2132    6093
 
AleI CACnn'nnGTG

Cuts at:   4388    4388 
   Size:       8225
 
AlwNI CAG_nnn'CTG

Cuts at:    748    1210    2204    2230    3145    5029    5530    7097     748 
   Size:        462     994      26     915    1884     501    1567    1876

  Fragments arranged by size: 

                1884    1876    1567     994     915     501     462      26
            
 
ApaI G_GGCC'C

Cuts at:   3008    3008 
   Size:       8225
 
ApaLI G'TGCA_C

Cuts at:   6995    6995 
   Size:       8225
 
ApoI r'AATT_y

Cuts at:    591     789    1071    1086    1140    1168    1392    1624    1732 
   Size:        198     282      15      54      28     224     232     108

Cuts at:   1732    1913    2500    2698    2716    5229    7578    7834     591 
   Size:        181     587     198      18    2513    2349     256     982

  Fragments arranged by size: 

                2513    2349     982     587     282     256     232     224
                 198     198     181     108      54      28      18      15
            
 
AseI AT'TA_AT

Cuts at:     20     161    2945    3977      20 
   Size:        141    2784    1032    4268

  Fragments arranged by size: 

                4268    2784    1032     141
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8225
 
AvaI C'yCGr_G

Cuts at:   1301    5245    1301 
   Size:       3944    4281
 
BamHI G'GATC_C

Cuts at:   5250    5250 
   Size:       8225
 
BanI G'GyrC_C

Cuts at:   2938    4652    5241    5655    5903    5938    2938 
   Size:       1714     589     414     248      35    5225

  Fragments arranged by size: 

                5225    1714     589     414     248      35
 
BanII G_rGCy'C

Cuts at:   3008    3722    5239    6269    3008 
   Size:        714    1517    1030    4964

  Fragments arranged by size: 

                4964    1517    1030     714
 
BbeI G_GCGC'C

Cuts at:   5659    5907    5659 
   Size:        248    7977
 
BbsI GAAGACnn'nnnn_

Cuts at:    497     667    2765    3297    5089     497 
   Size:        170    2098     532    1792    3633

  Fragments arranged by size: 

                3633    2098    1792     532     170
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   4288    4322    4288 
   Size:         34    8191
 
BciVI GTATCCnnnnn_n'

Cuts at:    278     533    1289    6123    6884    8225     278 
   Size:        255     756    4834     761    1341     278

  Fragments arranged by size: 

                4834    1341     761     756     278     255
 
BclI T'GATC_A

Cuts at:    402     710    5121    5745     402 
   Size:        308    4411     624    2882

  Fragments arranged by size: 

                4411    2882     624     308
 
BglI GCCn_nnn'nGGC

Cuts at:   5063    5063 
   Size:       8225
 
BglII A'GATC_T

Cuts at:   2290    3023    5740    7777    2290 
   Size:        733    2717    2037    2738

  Fragments arranged by size: 

                2738    2717    2037     733
 
BlpI GC'TnA_GC

Cuts at:   3475    5337    3475 
   Size:       1862    6363
 
Bme1580I G_kGCm'C

Cuts at:   3008    5850    5943    6999    3008 
   Size:       2842      93    1056    4234

  Fragments arranged by size: 

                4234    2842    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2244    2772    3011    4414    5437    5518    5853    7997    2244 
   Size:        528     239    1403    1023      81     335    2144    2472

  Fragments arranged by size: 

                2472    2144    1403    1023     528     335     239      81
            
 
BmtI G_CTAG'C

Cuts at:   2342    2668    2342 
   Size:        326    7899
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1207    6592    1207 
   Size:       5385    2840
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3616    4103    4315    4975    5035    3616 
   Size:        487     212     660      60    6806

  Fragments arranged by size: 

                6806     660     487     212      60
 
Bpu10I CC'TnA_GC

Cuts at:   1636    1636 
   Size:       8225
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1574    1838    2919    3056    5401    6280    6772    7070    1574 
   Size:        264    1081     137    2345     879     492     298    2729

  Fragments arranged by size: 

                2729    2345    1081     879     492     298     264     137
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   1108    2630    7929    1108 
   Size:       1522    5299    1404

  Fragments arranged by size: 

                5299    1522    1404
 
BsaAI yAC'GTr

Cuts at:   5578    5578 
   Size:       8225
 
BsaBI GATnn'nnATC

Cuts at:      6    5739    7776       6 
   Size:       5733    2037     455

  Fragments arranged by size: 

                5733    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   5656    5904    6606    8102    5656 
   Size:        248     702    1496    5779

  Fragments arranged by size: 

                5779    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2574    2632    4394    4481    5414    5935    6613    6887    7034 
   Size:         58    1762      87     933     521     678     274     147

Cuts at:   7034    7445    2574 
   Size:        411    3354

  Fragments arranged by size: 

                3354    1762     933     678     521     411     274     147
                  87      58
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2603    2627    2633    2657    3019    3049    2603 
   Size:         24       6      24     362      30    7779

  Fragments arranged by size: 

                7779     362      30      24      24       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    539    1096    1577    1801     539 
   Size:        557     481     224    6963

  Fragments arranged by size: 

                6963     557     481     224
 
BseYI C'CCAG_C

Cuts at:   2677    4616    4712    5650    6408    6985    7481    2677 
   Size:       1939      96     938     758     577     496    3421

  Fragments arranged by size: 

                3421    1939     938     758     577     496      96
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    242    2149    2228    2297     242 
   Size:       1907      79      69    6170

  Fragments arranged by size: 

                6170    1907      79      69
 
BsiEI CG_ry'CG

Cuts at:    104    5767    5813    7021     104 
   Size:       5663      46    1208    1308

  Fragments arranged by size: 

                5663    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   3722    4961    5239    6017    6207    6999    3722 
   Size:       1239     278     778     190     792    4948

  Fragments arranged by size: 

                4948    1239     792     778     278     190
 
BsmI GAATG_Cn'

Cuts at:    757    1372    3284    3891    4827     757 
   Size:        615    1912     607     936    4155

  Fragments arranged by size: 

                4155    1912     936     615     607
 
Bsp1286I G_dGCh'C

Cuts at:   3008    3722    4961    5239    5850    5943    6017    6207    6269 
   Size:        714    1239     278     611      93      74     190      62

Cuts at:   6269    6999    3008 
   Size:        730    4234

  Fragments arranged by size: 

                4234    1239     730     714     611     278     190      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   7445    7445 
   Size:       8225
 
BspHI T'CATG_A

Cuts at:   7401    7401 
   Size:       8225
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   3430    4290    5275    5489    5791    6172    3430 
   Size:        860     985     214     302     381    5483

  Fragments arranged by size: 

                5483     985     860     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    6517    6571    8055    8220      50 
   Size:       6467      54    1484     165      55

  Fragments arranged by size: 

                6467    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2358    6137    2358 
   Size:       3779    4446
 
BsrFI r'CCGG_y

Cuts at:   4481    6223    6404    6613    4481 
   Size:       1742     181     209    6093

  Fragments arranged by size: 

                6093    1742     209     181
 
BssSI C'ACGA_G

Cuts at:   6496    6854    6496 
   Size:        358    7867
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1210    2688    5602    1210 
   Size:       1478    2914    3833

  Fragments arranged by size: 

                3833    2914    1478
 
BstBI TT'CG_AA

Cuts at:   1675    4977    6586    1675 
   Size:       3302    1609    3314

  Fragments arranged by size: 

                3314    3302    1609
 
BstEII G'GTnAC_C

Cuts at:    271     271 
   Size:       8225
 
BstXI CCAn_nnnn'nTGG

Cuts at:    196    4616     196 
   Size:       4420    3805
 
BstYI r'GATC_y

Cuts at:      1    2226    2290    3023    3734    5250    5717    5740    6075 
   Size:       2225      64     733     711    1516     467      23     335

Cuts at:   6075    6321    7333    7419    7431    7777       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                2225    1516    1012     733     711     467     449     346
                 335     246      86      64      23      12
 
BtgI C'CryG_G

Cuts at:   3384    4907    4937    3384 
   Size:       1523      30    6672

  Fragments arranged by size: 

                6672    1523      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   6175    6356    6519    6175 
   Size:        181     163    7881

  Fragments arranged by size: 

                7881     181     163
 
BtsI GCAGTG_nn'

Cuts at:    569    1331    3703    4383    4395    4947    5468     569 
   Size:        762    2372     680      12     552     521    3326

  Fragments arranged by size: 

                3326    2372     762     680     552     521      12
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    446     481     446 
   Size:         35    8190
 
DraI TTT'AAA

Cuts at:   2996    3627    5224    2996 
   Size:        631    1597    5997

  Fragments arranged by size: 

                5997    1597     631
 
DrdI GACnn_nn'nnGTC

Cuts at:   5931    6789    5931 
   Size:        858    7367
 
EaeI y'GGCC_r

Cuts at:   4732    5810    5984    6375    6402    7747    4732 
   Size:       1078     174     391      27    1345    5210

  Fragments arranged by size: 

                5210    1345    1078     391     174      27
 
EagI C'GGCC_G

Cuts at:   5810    5810 
   Size:       8225
 
EarI CTCTTCn'nnn_

Cuts at:   1129    1778    2841    2852    3175    3806    4250    4277    4348 
   Size:        649    1063      11     323     631     444      27      71

Cuts at:   4348    4525    6248    6458    1129 
   Size:        177    1723     210    2896

  Fragments arranged by size: 

                2896    1723    1063     649     631     444     323     210
                 177      71      27      11
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   6433    6743    6889    8035    6433 
   Size:        310     146    1146    6623

  Fragments arranged by size: 

                6623    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   3720    5237    3720 
   Size:       1517    6708
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   2822    3616    4103    4315    4975    5035    5051    6050    6482 
   Size:        794     487     212     660      60      16     999     432

Cuts at:   6482    7229    7497    2822 
   Size:        747     268    3550

  Fragments arranged by size: 

                3550     999     794     747     660     487     432     268
                 212      60      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:   4214    4214 
   Size:       8225
 
EcoO109I rG'GnC_Cy

Cuts at:   3969    5364    3969 
   Size:       1395    6830
 
EcoRI G'AATT_C

Cuts at:   1086    5229    1086 
   Size:       4143    4082
 
EcoRV GAT'ATC

Cuts at:   1885    4148    1885 
   Size:       2263    5962
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4377    5056    6244    4377 
   Size:        679    1188    6358

  Fragments arranged by size: 

                6358    1188     679
 
FspI TGC'GCA

Cuts at:   6006    6006 
   Size:       8225
 
HaeII r_GCGC'y

Cuts at:   5659    5907    6929    7908    5659 
   Size:        248    1022     979    5976

  Fragments arranged by size: 

                5976    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    529     561     654     686    1075    1107    1567    1599    2224 
   Size:         32      93      32     389      32     460      32     625

Cuts at:   2224    2256    2603    2635    4542    4574    4888    4920    6067 
   Size:         32     347      32    1907      32     314      32    1147

Cuts at:   6067    6097    6099    6129     529 
   Size:         30       2      30    2625

  Fragments arranged by size: 

                2625    1907    1147     625     460     389     347     314
                  93      32      32      32      32      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:    992    3166    3813    5264     992 
   Size:       2174     647    1451    3953

  Fragments arranged by size: 

                3953    2174    1451     647
 
HindIII A'AGCT_T

Cuts at:   3621    3621 
   Size:       8225
 
KasI G'GCGC_C

Cuts at:   5655    5903    5655 
   Size:        248    7977
 
KpnI G_GTAC'C

Cuts at:   5245    5245 
   Size:       8225
 
MfeI C'AATT_G

Cuts at:   3425    3425 
   Size:       8225
 
MluI A'CGCG_T

Cuts at:   7658    7658 
   Size:       8225
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    446    2219    3380    4633    6896    7080     446 
   Size:       1773    1161    1253    2263     184    1591

  Fragments arranged by size: 

                2263    1773    1591    1253    1161     184
 
MscI TGG'CCA

Cuts at:   4734    5986    4734 
   Size:       1252    6973
 
MslI CAynn'nnrTG

Cuts at:    188    3499    3584    3884    4388    4721    6214    6341     188 
   Size:       3311      85     300     504     333    1493     127    2072

  Fragments arranged by size: 

                3311    2072    1493     504     333     300     127      85
            
 
MspA1I CmG'CkG

Cuts at:   3509    4369    4741    5336    5650    6010    7023    7268    3509 
   Size:        860     372     595     314     360    1013     245    4466

  Fragments arranged by size: 

                4466    1013     860     595     372     360     314     245
            
 
NaeI GCC'GGC

Cuts at:   6406    6406 
   Size:       8225
 
NarI GG'CG_CC

Cuts at:   5656    5904    5656 
   Size:        248    7977
 
NcoI C'CATG_G

Cuts at:   3384    4937    3384 
   Size:       1553    6672
 
NdeI CA'TA_TG

Cuts at:   2441    2441 
   Size:       8225
 
NgoMIV G'CCGG_C

Cuts at:   6404    6404 
   Size:       8225
 
NheI G'CTAG_C

Cuts at:   2338    2664    2338 
   Size:        326    7899
 
NspI r_CATG'y

Cuts at:   1605    5278    5778    6685    1605 
   Size:       3673     500     907    3145

  Fragments arranged by size: 

                3673    3145     907     500
 
PasI CC'CwG_GG

Cuts at:   1179    1179 
   Size:       8225
 
PciI A'CATG_T

Cuts at:   6681    6681 
   Size:       8225
 
PflMI CCAn_nnn'nTGG

Cuts at:   3192    3897    4741    4953    3192 
   Size:        705     844     212    6464

  Fragments arranged by size: 

                6464     844     705     212
 
PfoI T'CCnGG_A

Cuts at:   3593    3593 
   Size:       8225
 
PmeI GTTT'AAAC

Cuts at:   5224    5224 
   Size:       8225
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   7364    7396    7364 
   Size:         32    8193
 
PpuMI rG'GwC_Cy

Cuts at:   3969    3969 
   Size:       8225
 
PsiI TTA'TAA

Cuts at:   4138    4138 
   Size:       8225
 
PspOMI G'GGCC_C

Cuts at:   3004    3004 
   Size:       8225
 
PstI C_TGCA'G

Cuts at:    248     576    4390    5272    5957     248 
   Size:        328    3814     882     685    2516

  Fragments arranged by size: 

                3814    2516     882     685     328
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       8225
 
PvuII CAG'CTG

Cuts at:   3509    4741    5650    6010    3509 
   Size:       1232     909     360    5724

  Fragments arranged by size: 

                5724    1232     909     360
 
RsrII CG'GwC_CG

Cuts at:   6420    6420 
   Size:       8225
 
SacI G_AGCT'C

Cuts at:   3722    5239    3722 
   Size:       1517    6708
 
SalI G'TCGA_C

Cuts at:   5262    5262 
   Size:       8225
 
SapI GCTCTTCn'nnn_

Cuts at:   4250    6248    6458    4250 
   Size:       1998     210    6017

  Fragments arranged by size: 

                6017    1998     210
 
SbfI CC_TGCA'GG

Cuts at:   5272    5272 
   Size:       8225
 
ScaI AGT'ACT

Cuts at:   7742    7742 
   Size:       8225
 
SexAI A'CCwGG_T

Cuts at:   4343    4343 
   Size:       8225
 
SfcI C'TryA_G

Cuts at:     33     244     266     572    2067    2564    4128    4386    5268 
   Size:        211      22     306    1495     497    1564     258     882

Cuts at:   5268    5953    6946    7137      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1564    1495    1121     993     882     685     497     306
                 258     211     191      22
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   5063    5063 
   Size:       8225
 
SfoI GGC'GCC

Cuts at:   5657    5905    5657 
   Size:        248    7977
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8225
 
SmaI CCC'GGG

Cuts at:   5247    5247 
   Size:       8225
 
SmlI C'TyrA_G

Cuts at:   1301    1589    1853    2898    3071    3285    5380    6295    6787 
   Size:        288     264    1045     173     214    2095     915     492

Cuts at:   6787    7049    1301 
   Size:        262    2477

  Fragments arranged by size: 

                2477    2095    1045     915     492     288     264     262
                 214     173
 
SpeI A'CTAG_T

Cuts at:     67    3943      67 
   Size:       3876    4349
 
SphI G_CATG'C

Cuts at:   5278    5778    5278 
   Size:        500    7725
 
SspI AAT'ATT

Cuts at:    633    3995    4203    4845     633 
   Size:       3362     208     642    4013

  Fragments arranged by size: 

                4013    3362     642     208
 
StuI AGG'CCT

Cuts at:   5066    5066 
   Size:       8225
 
StyI C'CwwG_G

Cuts at:   2312    3384    3902    4829    4937    5359    5713    2312 
   Size:       1072     518     927     108     422     354    4824

  Fragments arranged by size: 

                4824    1072     927     518     422     354     108
 
SwaI ATTT'AAAT

Cuts at:   2996    2996 
   Size:       8225
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1608    2045    4081    5808    5941    6400    6609    1608 
   Size:        437    2036    1727     133     459     209    3224

  Fragments arranged by size: 

                3224    2036    1727     459     437     209     133
 
TatI w'GTAC_w

Cuts at:   4290    4399    7740    4290 
   Size:        109    3341    4775

  Fragments arranged by size: 

                4775    3341     109
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1925    1957    2627    2659    7742    7774    1925 
   Size:         32     670      32    5083      32    2376

  Fragments arranged by size: 

                5083    2376     670      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   6022    6022 
   Size:       8225
 
XbaI T'CTAG_A

Cuts at:   2583    5256    2583 
   Size:       2673    5552
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2231    5720    2231 
   Size:       3489    4736
 
XhoI C'TCGA_G

Cuts at:   1301    1301 
   Size:       8225
 
XmaI C'CCGG_G

Cuts at:   5245    5245 
   Size:       8225
 
XmnI GAAnn'nnTTC

Cuts at:    789    1370    1392    4357    4758     789 
   Size:        581      22    2965     401    4256

  Fragments arranged by size: 

                4256    2965     581     401      22
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI     PsiI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AlfI     AloI     AscI    AvrII     BaeI
    BbvCI    BfrBI    BmgBI    BsiWI    BsmBI    BsrGI   BssHII  BstZ17I
   Bsu36I     ClaI   DraIII     FseI    FspAI     HpaI     NotI     NruI
     NsiI     PacI     PmlI    PshAI    PspXI     PsrI    SacII    SanDI
    SgrAI    SnaBI     SrfI     ZraI
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