Restriction map for pF1KA0580
 (Linear) (Six-base) MAPSORT of: KIAA0580.seq  Check: 4116  from: 1  to: 5112



KIAA0580.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4182    5112 
   Size:       4182     930
 
AccI GT'mk_AC

Cuts at:      0    2815    5112 
   Size:       2815    2297
 
AclI AA'CG_TT

Cuts at:      0    4650    5112 
   Size:       4650     462
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2714    4943    5112 
   Size:       2714    2229     169

  Fragments arranged by size: 

                2714    2229     169
 
AflII C'TTAA_G

Cuts at:      0    3177    5112 
   Size:       3177    1935
 
AflIII A'CryG_T

Cuts at:      0    2934    5112 
   Size:       2934    2178
 
AgeI A'CCGG_T

Cuts at:      0    4373    5112 
   Size:       4373     739
 
AleI CACnn'nnGTG

Cuts at:      0    4280    5112 
   Size:       4280     832
 
AlwNI CAG_nnn'CTG

Cuts at:      0     640    1102    2096    2122    3037    4921    5112 
   Size:        640     462     994      26     915    1884     191

  Fragments arranged by size: 

                1884     994     915     640     462     191      26
 
ApaI G_GGCC'C

Cuts at:      0    2900    5112 
   Size:       2900    2212
 
ApoI r'AATT_y

Cuts at:      0     483     681     963     978    1032    1060    1284    1516 
   Size:        483     198     282      15      54      28     224     232

Cuts at:   1516    1624    1805    2392    2590    2608    5112 
   Size:        108     181     587     198      18    2504

  Fragments arranged by size: 

                2504     587     483     282     232     224     198     198
                 181     108      54      28      18      15
 
AseI AT'TA_AT

Cuts at:      0      53    2837    3869    5112 
   Size:         53    2784    1032    1243

  Fragments arranged by size: 

                2784    1243    1032      53
 
AvaI C'yCGr_G

Cuts at:      0    1193    5112 
   Size:       1193    3919
 
BanI G'GyrC_C

Cuts at:      0    2830    4544    5112 
   Size:       2830    1714     568

  Fragments arranged by size: 

                2830    1714     568
 
BanII G_rGCy'C

Cuts at:      0    2900    3614    5112 
   Size:       2900     714    1498

  Fragments arranged by size: 

                2900    1498     714
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     389     559    2657    3189    4981    5112 
   Size:        389     170    2098     532    1792     131

  Fragments arranged by size: 

                2098    1792     532     389     170     131
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4180    4214    5112 
   Size:       4180      34     898

  Fragments arranged by size: 

                4180     898      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     170     425    1181    5112 
   Size:        170     255     756    3931

  Fragments arranged by size: 

                3931     756     255     170
 
BclI T'GATC_A

Cuts at:      0     294     602    5013    5112 
   Size:        294     308    4411      99

  Fragments arranged by size: 

                4411     308     294      99
 
BglI GCCn_nnn'nGGC

Cuts at:      0    4955    5112 
   Size:       4955     157
 
BglII A'GATC_T

Cuts at:      0    2182    2915    5112 
   Size:       2182     733    2197

  Fragments arranged by size: 

                2197    2182     733
 
BlpI GC'TnA_GC

Cuts at:      0    3367    5112 
   Size:       3367    1745
 
Bme1580I G_kGCm'C

Cuts at:      0    2900    5112 
   Size:       2900    2212
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2136    2664    2903    4306    5112 
   Size:       2136     528     239    1403     806

  Fragments arranged by size: 

                2136    1403     806     528     239
 
BmtI G_CTAG'C

Cuts at:      0    2234    2560    5112 
   Size:       2234     326    2552

  Fragments arranged by size: 

                2552    2234     326
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1099    5112 
   Size:       1099    4013
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3508    3995    4207    4867    4927    5112 
   Size:       3508     487     212     660      60     185

  Fragments arranged by size: 

                3508     660     487     212     185      60
 
Bpu10I CC'TnA_GC

Cuts at:      0    1528    5112 
   Size:       1528    3584
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1466    1730    2811    2948    5112 
   Size:       1466     264    1081     137    2164

  Fragments arranged by size: 

                2164    1466    1081     264     137
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1000    2522    5112 
   Size:       1000    1522    2590

  Fragments arranged by size: 

                2590    1522    1000
 
BsaWI w'CCGG_w

Cuts at:      0    2466    2524    4286    4373    5112 
   Size:       2466      58    1762      87     739

  Fragments arranged by size: 

                2466    1762     739      87      58
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2495    2519    2525    2549    2911    2941    5112 
   Size:       2495      24       6      24     362      30    2171

  Fragments arranged by size: 

                2495    2171     362      30      24      24       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     431     988    1469    1693    5112 
   Size:        431     557     481     224    3419

  Fragments arranged by size: 

                3419     557     481     431     224
 
BseYI C'CCAG_C

Cuts at:      0    2569    4508    4604    5112 
   Size:       2569    1939      96     508

  Fragments arranged by size: 

                2569    1939     508      96
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134    2041    2120    2189    5112 
   Size:        134    1907      79      69    2923

  Fragments arranged by size: 

                2923    1907     134      79      69
 
BsiHKAI G_wGCw'C

Cuts at:      0    3614    4853    5112 
   Size:       3614    1239     259

  Fragments arranged by size: 

                3614    1239     259
 
BsmI GAATG_Cn'

Cuts at:      0     649    1264    3176    3783    4719    5112 
   Size:        649     615    1912     607     936     393

  Fragments arranged by size: 

                1912     936     649     615     607     393
 
Bsp1286I G_dGCh'C

Cuts at:      0    2900    3614    4853    5112 
   Size:       2900     714    1239     259

  Fragments arranged by size: 

                2900    1239     714     259
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3322    4182    5112 
   Size:       3322     860     930

  Fragments arranged by size: 

                3322     930     860
 
BsrDI GCAATG_nn'

Cuts at:      0    2250    5112 
   Size:       2250    2862
 
BsrFI r'CCGG_y

Cuts at:      0    4373    5112 
   Size:       4373     739
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1102    2580    5112 
   Size:       1102    1478    2532

  Fragments arranged by size: 

                2532    1478    1102
 
BstBI TT'CG_AA

Cuts at:      0    1567    4869    5112 
   Size:       1567    3302     243

  Fragments arranged by size: 

                3302    1567     243
 
BstEII G'GTnAC_C

Cuts at:      0     163    5112 
   Size:        163    4949
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      88    4508    5112 
   Size:         88    4420     604

  Fragments arranged by size: 

                4420     604      88
 
BstYI r'GATC_y

Cuts at:      0    2118    2182    2915    3626    5112 
   Size:       2118      64     733     711    1486

  Fragments arranged by size: 

                2118    1486     733     711      64
 
BtgI C'CryG_G

Cuts at:      0    3276    4799    4829    5112 
   Size:       3276    1523      30     283

  Fragments arranged by size: 

                3276    1523     283      30
 
BtsI GCAGTG_nn'

Cuts at:      0     461    1223    3595    4275    4287    4839    5112 
   Size:        461     762    2372     680      12     552     273

  Fragments arranged by size: 

                2372     762     680     552     461     273      12
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     338     373    5112 
   Size:        338      35    4739

  Fragments arranged by size: 

                4739     338      35
 
DraI TTT'AAA

Cuts at:      0    2888    3519    5112 
   Size:       2888     631    1593

  Fragments arranged by size: 

                2888    1593     631
 
EaeI y'GGCC_r

Cuts at:      0    4624    5112 
   Size:       4624     488
 
EarI CTCTTCn'nnn_

Cuts at:      0    1021    1670    2733    2744    3067    3698    4142    4169 
   Size:       1021     649    1063      11     323     631     444      27

Cuts at:   4169    4240    4417    5112 
   Size:         71     177     695

  Fragments arranged by size: 

                1063    1021     695     649     631     444     323     177
                  71      27      11
 
EcoICRI GAG'CTC

Cuts at:      0    3612    5112 
   Size:       3612    1500
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2714    3508    3995    4207    4867    4927    4943    5112 
   Size:       2714     794     487     212     660      60      16     169

  Fragments arranged by size: 

                2714     794     660     487     212     169      60      16
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    4106    5112 
   Size:       4106    1006
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3861    5112 
   Size:       3861    1251
 
EcoRI G'AATT_C

Cuts at:      0     978    5112 
   Size:        978    4134
 
EcoRV GAT'ATC

Cuts at:      0    1777    4040    5112 
   Size:       1777    2263    1072

  Fragments arranged by size: 

                2263    1777    1072
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4269    4948    5112 
   Size:       4269     679     164

  Fragments arranged by size: 

                4269     679     164
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     421     453     546     578     967     999    1459    1491 
   Size:        421      32      93      32     389      32     460      32

Cuts at:   1491    2116    2148    2495    2527    4434    4466    4780    4812 
   Size:        625      32     347      32    1907      32     314      32

Cuts at:   4812    5112 
   Size:        300

  Fragments arranged by size: 

                1907     625     460     421     389     347     314     300
                  93      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     884    3058    3705    5112 
   Size:        884    2174     647    1407

  Fragments arranged by size: 

                2174    1407     884     647
 
HindIII A'AGCT_T

Cuts at:      0    3513    5112 
   Size:       3513    1599
 
MfeI C'AATT_G

Cuts at:      0    3317    5112 
   Size:       3317    1795
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     338    2111    3272    4525    5112 
   Size:        338    1773    1161    1253     587

  Fragments arranged by size: 

                1773    1253    1161     587     338
 
MscI TGG'CCA

Cuts at:      0    4626    5112 
   Size:       4626     486
 
MslI CAynn'nnrTG

Cuts at:      0      80    3391    3476    3776    4280    4613    5112 
   Size:         80    3311      85     300     504     333     499

  Fragments arranged by size: 

                3311     504     499     333     300      85      80
 
MspA1I CmG'CkG

Cuts at:      0    3401    4261    4633    5112 
   Size:       3401     860     372     479

  Fragments arranged by size: 

                3401     860     479     372
 
NcoI C'CATG_G

Cuts at:      0    3276    4829    5112 
   Size:       3276    1553     283

  Fragments arranged by size: 

                3276    1553     283
 
NdeI CA'TA_TG

Cuts at:      0    2333    5112 
   Size:       2333    2779
 
NheI G'CTAG_C

Cuts at:      0    2230    2556    5112 
   Size:       2230     326    2556

  Fragments arranged by size: 

                2556    2230     326
 
NspI r_CATG'y

Cuts at:      0    1497    5112 
   Size:       1497    3615
 
PasI CC'CwG_GG

Cuts at:      0    1071    5112 
   Size:       1071    4041
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3084    3789    4633    4845    5112 
   Size:       3084     705     844     212     267

  Fragments arranged by size: 

                3084     844     705     267     212
 
PfoI T'CCnGG_A

Cuts at:      0    3485    5112 
   Size:       3485    1627
 
PpuMI rG'GwC_Cy

Cuts at:      0    3861    5112 
   Size:       3861    1251
 
PsiI TTA'TAA

Cuts at:      0    4030    5112 
   Size:       4030    1082
 
PspOMI G'GGCC_C

Cuts at:      0    2896    5112 
   Size:       2896    2216
 
PstI C_TGCA'G

Cuts at:      0     140     468    4282    5112 
   Size:        140     328    3814     830

  Fragments arranged by size: 

                3814     830     328     140
 
PvuII CAG'CTG

Cuts at:      0    3401    4633    5112 
   Size:       3401    1232     479

  Fragments arranged by size: 

                3401    1232     479
 
SacI G_AGCT'C

Cuts at:      0    3614    5112 
   Size:       3614    1498
 
SapI GCTCTTCn'nnn_

Cuts at:      0    4142    5112 
   Size:       4142     970
 
SexAI A'CCwGG_T

Cuts at:      0    4235    5112 
   Size:       4235     877
 
SfcI C'TryA_G

Cuts at:      0     136     158     464    1959    2456    4020    4278    5112 
   Size:        136      22     306    1495     497    1564     258     834

  Fragments arranged by size: 

                1564    1495     834     497     306     258     136      22
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    4955    5112 
   Size:       4955     157
 
SmlI C'TyrA_G

Cuts at:      0    1193    1481    1745    2790    2963    3177    5112 
   Size:       1193     288     264    1045     173     214    1935

  Fragments arranged by size: 

                1935    1193    1045     288     264     214     173
 
SpeI A'CTAG_T

Cuts at:      0    3835    5112 
   Size:       3835    1277
 
SspI AAT'ATT

Cuts at:      0     525    3887    4095    4737    5112 
   Size:        525    3362     208     642     375

  Fragments arranged by size: 

                3362     642     525     375     208
 
StuI AGG'CCT

Cuts at:      0    4958    5112 
   Size:       4958     154
 
StyI C'CwwG_G

Cuts at:      0    2204    3276    3794    4721    4829    5112 
   Size:       2204    1072     518     927     108     283

  Fragments arranged by size: 

                2204    1072     927     518     283     108
 
SwaI ATTT'AAAT

Cuts at:      0    2888    5112 
   Size:       2888    2224
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1500    1937    3973    5112 
   Size:       1500     437    2036    1139

  Fragments arranged by size: 

                2036    1500    1139     437
 
TatI w'GTAC_w

Cuts at:      0    4182    4291    5112 
   Size:       4182     109     821

  Fragments arranged by size: 

                4182     821     109
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1817    1849    2519    2551    5112 
   Size:       1817      32     670      32    2561

  Fragments arranged by size: 

                2561    1817     670      32      32
 
XbaI T'CTAG_A

Cuts at:      0    2475    5112 
   Size:       2475    2637
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2123    5112 
   Size:       2123    2989
 
XhoI C'TCGA_G

Cuts at:      0    1193    5112 
   Size:       1193    3919
 
XmnI GAAnn'nnTTC

Cuts at:      0     681    1262    1284    4249    4650    5112 
   Size:        681     581      22    2965     401     462

  Fragments arranged by size: 

                2965     681     581     462     401      22
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI    AflII   AflIII     AgeI     AleI
    AlwNI     ApaI     ApoI     AseI     AvaI     BanI    BanII     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI
    BsrFI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI     BtsI
    CspCI     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     MfeI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NspI     PasI
    PflMI     PfoI    PpuMI     PsiI   PspOMI     PstI    PvuII     SacI
     SapI    SexAI     SfcI     SfiI     SmlI     SpeI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI     AhdI     AlfI     AloI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI    BfrBI    BmgBI
    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrGI   BssHII    BssSI  BstZ17I   Bsu36I    BtgZI     ClaI
   DraIII     DrdI     EagI     EciI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MluI     NaeI     NarI   NgoMIV     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
  Tth111I     XmaI     ZraI
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