Restriction map for pF1KA0419
 (Linear) (Six-base) MAPSORT of: KIAA0419.seq  Check: 203  from: 1  to: 2973



KIAA0419.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AccI GT'mk_AC

Cuts at:      0    2436    2892    2973 
   Size:       2436     456      81

  Fragments arranged by size: 

                2436     456      81
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557     667    1361    1376    2479    2548    2830    2973 
   Size:        557     110     694      15    1103      69     282     143

  Fragments arranged by size: 

                1103     694     557     282     143     110      69      15
            
 
AleI CACnn'nnGTG

Cuts at:      0    1258    2973 
   Size:       1258    1715
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     955    2973 
   Size:        955    2018
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     975    1007    2973 
   Size:        975      32    1966

  Fragments arranged by size: 

                1966     975      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     690    1490    2973 
   Size:        690     800    1483

  Fragments arranged by size: 

                1483     800     690
 
ApoI r'AATT_y

Cuts at:      0    2211    2392    2494    2806    2973 
   Size:       2211     181     102     312     167

  Fragments arranged by size: 

                2211     312     181     167     102
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     673    1137    2961    2973 
   Size:        673     464    1824      12

  Fragments arranged by size: 

                1824     673     464      12
 
BclI T'GATC_A

Cuts at:      0     300    2973 
   Size:        300    2673
 
BlpI GC'TnA_GC

Cuts at:      0    2015    2973 
   Size:       2015     958
 
Bme1580I G_kGCm'C

Cuts at:      0    1265    2973 
   Size:       1265    1708
 
BmtI G_CTAG'C

Cuts at:      0     956    2973 
   Size:        956    2017
 
Bpu10I CC'TnA_GC

Cuts at:      0    2692    2973 
   Size:       2692     281
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     905    1558    2171    2348    2534    2973 
   Size:        905     653     613     177     186     439

  Fragments arranged by size: 

                 905     653     613     439     186     177
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2366    2973 
   Size:       2366     607
 
BsaBI GATnn'nnATC

Cuts at:      0     299    2973 
   Size:        299    2674
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2056    2086    2973 
   Size:       2056      30     887

  Fragments arranged by size: 

                2056     887      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19    1939    2973 
   Size:         19    1920    1034

  Fragments arranged by size: 

                1920    1034      19
 
BseYI C'CCAG_C

Cuts at:      0     427    1991    2973 
   Size:        427    1564     982

  Fragments arranged by size: 

                1564     982     427
 
BsmI GAATG_Cn'

Cuts at:      0    2798    2871    2973 
   Size:       2798      73     102

  Fragments arranged by size: 

                2798     102      73
 
Bsp1286I G_dGCh'C

Cuts at:      0    1265    2973 
   Size:       1265    1708
 
BspHI T'CATG_A

Cuts at:      0    2610    2661    2973 
   Size:       2610      51     312

  Fragments arranged by size: 

                2610     312      51
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     201    2973 
   Size:        201    2772
 
BsrDI GCAATG_nn'

Cuts at:      0     523    1226    2096    2973 
   Size:        523     703     870     877

  Fragments arranged by size: 

                 877     870     703     523
 
BssSI C'ACGA_G

Cuts at:      0     239    2720    2973 
   Size:        239    2481     253

  Fragments arranged by size: 

                2481     253     239
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     286    1845    2973 
   Size:        286    1559    1128

  Fragments arranged by size: 

                1559    1128     286
 
BstBI TT'CG_AA

Cuts at:      0     961    2973 
   Size:        961    2012
 
BstYI r'GATC_y

Cuts at:      0     633     663    1870    2973 
   Size:        633      30    1207    1103

  Fragments arranged by size: 

                1207    1103     633      30
 
Bsu36I CC'TnA_GG

Cuts at:      0    1865    2973 
   Size:       1865    1108
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1513    2973 
   Size:       1513    1460
 
BtsI GCAGTG_nn'

Cuts at:      0     136    2135    2417    2973 
   Size:        136    1999     282     556

  Fragments arranged by size: 

                1999     556     282     136
 
DraI TTT'AAA

Cuts at:      0    1023    2973 
   Size:       1023    1950
 
EaeI y'GGCC_r

Cuts at:      0     987    2930    2973 
   Size:        987    1943      43

  Fragments arranged by size: 

                1943     987      43
 
EarI CTCTTCn'nnn_

Cuts at:      0     576    1963    2051    2337    2973 
   Size:        576    1387      88     286     636

  Fragments arranged by size: 

                1387     636     576     286      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557     667    1361    1376    2479    2548    2830    2973 
   Size:        557     110     694      15    1103      69     282     143

  Fragments arranged by size: 

                1103     694     557     282     143     110      69      15
            
 
EcoRI G'AATT_C

Cuts at:      0    2494    2973 
   Size:       2494     479
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     610    2443    2973 
   Size:        610    1833     530

  Fragments arranged by size: 

                1833     610     530
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     110     142    2973 
   Size:        110      32    2831

  Fragments arranged by size: 

                2831     110      32
 
HincII GTy'rAC

Cuts at:      0     216     839    1451    1884    2973 
   Size:        216     623     612     433    1089

  Fragments arranged by size: 

                1089     623     612     433     216
 
HindIII A'AGCT_T

Cuts at:      0     227     412     554    2973 
   Size:        227     185     142    2419

  Fragments arranged by size: 

                2419     227     185     142
 
HpaI GTT'AAC

Cuts at:      0     216    1451    1884    2973 
   Size:        216    1235     433    1089

  Fragments arranged by size: 

                1235    1089     433     216
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2093    2973 
   Size:       2093     880
 
MscI TGG'CCA

Cuts at:      0     989    2973 
   Size:        989    1984
 
MslI CAynn'nnrTG

Cuts at:      0     937    1152    1258    1699    2973 
   Size:        937     215     106     441    1274

  Fragments arranged by size: 

                1274     937     441     215     106
 
MspA1I CmG'CkG

Cuts at:      0    2098    2638    2737    2973 
   Size:       2098     540      99     236

  Fragments arranged by size: 

                2098     540     236      99
 
NdeI CA'TA_TG

Cuts at:      0    1080    1521    2973 
   Size:       1080     441    1452

  Fragments arranged by size: 

                1452    1080     441
 
NheI G'CTAG_C

Cuts at:      0     952    2973 
   Size:        952    2021
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1555    2973 
   Size:       1555    1418
 
PfoI T'CCnGG_A

Cuts at:      0     245    1138    2973 
   Size:        245     893    1835

  Fragments arranged by size: 

                1835     893     245
 
PshAI GACnn'nnGTC

Cuts at:      0     241    2973 
   Size:        241    2732
 
PsiI TTA'TAA

Cuts at:      0    1168    2973 
   Size:       1168    1805
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0       1      33    2973 
   Size:          1      32    2940

  Fragments arranged by size: 

                2940      32       1
 
PstI C_TGCA'G

Cuts at:      0     408    1606    2412    2973 
   Size:        408    1198     806     561

  Fragments arranged by size: 

                1198     806     561     408
 
PvuII CAG'CTG

Cuts at:      0    2638    2737    2973 
   Size:       2638      99     236

  Fragments arranged by size: 

                2638     236      99
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2051    2337    2973 
   Size:       2051     286     636

  Fragments arranged by size: 

                2051     636     286
 
SfcI C'TryA_G

Cuts at:      0     404    1602    2408    2973 
   Size:        404    1198     806     565

  Fragments arranged by size: 

                1198     806     565     404
 
SmlI C'TyrA_G

Cuts at:      0     920    1573    2186    2363    2549    2973 
   Size:        920     653     613     177     186     424

  Fragments arranged by size: 

                 920     653     613     424     186     177
 
SpeI A'CTAG_T

Cuts at:      0    1906    2302    2973 
   Size:       1906     396     671

  Fragments arranged by size: 

                1906     671     396
 
SspI AAT'ATT

Cuts at:      0     534    2973 
   Size:        534    2439
 
StuI AGG'CCT

Cuts at:      0    2334    2973 
   Size:       2334     639
 
StyI C'CwwG_G

Cuts at:      0     906    2856    2973 
   Size:        906    1950     117

  Fragments arranged by size: 

                1950     906     117
 
Tth111I GACn'n_nGTC

Cuts at:      0     737    2973 
   Size:        737    2236
 
XbaI T'CTAG_A

Cuts at:      0     730    1002    2973 
   Size:        730     272    1971

  Fragments arranged by size: 

                1971     730     272
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2285    2973 
   Size:       2285     688
 
XmnI GAAnn'nnTTC

Cuts at:      0     557    1134    1908    2973 
   Size:        557     577     774    1065

  Fragments arranged by size: 

                1065     774     577     557
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AlfI     AloI    AlwNI     ApoI     BbsI
     BclI     BlpI Bme1580I     BmtI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaXI    BseRI    BseYI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BssSI   BstAPI    BstBI    BstYI   Bsu36I    BtgZI     BtsI     DraI
     EaeI     EarI  Eco57MI    EcoRI     FalI    Hin4I   HincII  HindIII
     HpaI     MmeI     MscI     MslI   MspA1I     NdeI     NheI    PflMI
     PfoI    PshAI     PsiI     PsrI     PstI    PvuII     SapI     SfcI
     SmlI     SpeI     SspI     StuI     StyI  Tth111I     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII    BmgBI     BmrI     BplI     BpmI    BsaAI
    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI    BsrGI   BssHII   BstEII    BstXI  BstZ17I     BtgI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SwaI    TaqII     TatI     TstI
     XhoI     XmaI     ZraI
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