Restriction map for pF1KA0343
 (Circular) (Six-base) MAPSORT of: pF1KA0343.seq  Check: 6065  from: 1  to: 6653



pF1KA0343  6653 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1434    3207    1434 
   Size:       1773    4880
 
AatII G_ACGT'C

Cuts at:   2742    2742 
   Size:       6653
 
AccI GT'mk_AC

Cuts at:   3691    3691 
   Size:       6653
 
Acc65I G'GTAC_C

Cuts at:   3669    3669 
   Size:       6653
 
AclI AA'CG_TT

Cuts at:   6493    6493 
   Size:       6653
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1816    2165    2374    3443    3611    4478    4910    5657    5925 
   Size:        349     209    1069     168     867     432     747     268

Cuts at:   5925    1816 
   Size:       2544

  Fragments arranged by size: 

                2544    1069     867     747     432     349     268     209
                 168
 
AfeI AGC'GCT

Cuts at:    139    1935     139 
   Size:       1796    4857
 
AflIII A'CryG_T

Cuts at:   1621    1892    1894    4005    5109    6086    1621 
   Size:        271       2    2111    1104     977    2188

  Fragments arranged by size: 

                2188    2111    1104     977     271       2
 
AgeI A'CCGG_T

Cuts at:   5041    5041 
   Size:       6653
 
AleI CACnn'nnGTG

Cuts at:    543     921    1897    2305     543 
   Size:        378     976     408    4891

  Fragments arranged by size: 

                4891     976     408     378
 
AlwNI CAG_nnn'CTG

Cuts at:   1054    2329    2520    3958    5525    1054 
   Size:       1275     191    1438    1567    2182

  Fragments arranged by size: 

                2182    1567    1438    1275     191
 
ApaI G_GGCC'C

Cuts at:   2863    2863 
   Size:       6653
 
ApaLI G'TGCA_C

Cuts at:    191    2641    5423     191 
   Size:       2450    2782    1421

  Fragments arranged by size: 

                2782    2450    1421
 
ApoI r'AATT_y

Cuts at:   1583    2086    3065    3099    3105    3636    3657    6006    6262 
   Size:        503     979      34       6     531      21    2349     256

Cuts at:   6262    1583 
   Size:       1974

  Fragments arranged by size: 

                2349    1974     979     531     503     256      34      21
                   6
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6653
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6653
 
AvaI C'yCGr_G

Cuts at:    287    2542    3673     287 
   Size:       2255    1131    3267

  Fragments arranged by size: 

                3267    2255    1131
 
BamHI G'GATC_C

Cuts at:   2967    3678    2967 
   Size:        711    5942
 
BanI G'GyrC_C

Cuts at:   2132    2408    3612    3669    4083    4331    4366    2132 
   Size:        276    1204      57     414     248      35    4419

  Fragments arranged by size: 

                4419    1204     414     276     248      57      35
 
BanII G_rGCy'C

Cuts at:   2810    2863    3667    4697    2810 
   Size:         53     804    1030    4766

  Fragments arranged by size: 

                4766    1030     804      53
 
BbeI G_GCGC'C

Cuts at:   2412    4087    4335    2412 
   Size:       1675     248    4730

  Fragments arranged by size: 

                4730    1675     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    233     755    2582    2716    3428     233 
   Size:        522    1827     134     712    3458

  Fragments arranged by size: 

                3458    1827     712     522     134
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    6619
 
BciVI GTATCCnnnnn_n'

Cuts at:   2469    4551    5312    6653    2469 
   Size:       2082     761    1341    2469

  Fragments arranged by size: 

                2469    2082    1341     761
 
BclI T'GATC_A

Cuts at:   1764    2504    4173    1764 
   Size:        740    1669    4244

  Fragments arranged by size: 

                4244    1669     740
 
BfrBI ATG'CAT

Cuts at:   1084    1084 
   Size:       6653
 
BglII A'GATC_T

Cuts at:   4168    6205    4168 
   Size:       2037    4616
 
BlpI GC'TnA_GC

Cuts at:   1403    1942    3765    1403 
   Size:        539    1823    4291

  Fragments arranged by size: 

                4291    1823     539
 
Bme1580I G_kGCm'C

Cuts at:    161     195    2645    2863    4278    4371    5427     161 
   Size:         34    2450     218    1415      93    1056    1387

  Fragments arranged by size: 

                2450    1415    1387    1056     218      93      34
 
BmrI ACTGGGnnnn_n'

Cuts at:    969    1811    2326    2647    2655    2900    3865    3946    4281 
   Size:        842     515     321       8     245     965      81     335

Cuts at:   4281    6425     969 
   Size:       2144    1197

  Fragments arranged by size: 

                2144    1197     965     842     515     335     321     245
                  81       8
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    707    2596    3561    5020     707 
   Size:       1889     965    1459    2340

  Fragments arranged by size: 

                2340    1889    1459     965
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    851     940    1952    2591    2876     851 
   Size:         89    1012     639     285    4628

  Fragments arranged by size: 

                4628    1012     639     285      89
 
Bpu10I CC'TnA_GC

Cuts at:   2631    2631 
   Size:       6653
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    677    3829    4708    5200    5498     677 
   Size:       3152     879     492     298    1832

  Fragments arranged by size: 

                3152    1832     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    445    1496    6357     445 
   Size:       1051    4861     741

  Fragments arranged by size: 

                4861    1051     741
 
BsaAI yAC'GTr

Cuts at:   1622    1895    2193    2348    4006    1622 
   Size:        273     298     155    1658    4269

  Fragments arranged by size: 

                4269    1658     298     273     155
 
BsaBI GATnn'nnATC

Cuts at:      6    4167    6204       6 
   Size:       4161    2037     455

  Fragments arranged by size: 

                4161    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2254    2409    2739    4084    4332    5034    6530    2254 
   Size:        155     330    1345     248     702    1496    2377

  Fragments arranged by size: 

                2377    1496    1345     702     330     248     155
 
BsaWI w'CCGG_w

Cuts at:   3842    4363    5041    5315    5462    5873    3842 
   Size:        521     678     274     147     411    4622

  Fragments arranged by size: 

                4622     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    488     518    1909    1939    1950    1956    1980    1986    3503 
   Size:         30    1391      30      11       6      24       6    1517

Cuts at:   3503    3533     488 
   Size:         30    3608

  Fragments arranged by size: 

                3608    1517    1391      30      30      30      24      11
                   6       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1484    1484 
   Size:       6653
 
BseYI C'CCAG_C

Cuts at:   1273    2171    2245    2862    4078    4836    5413    5909    1273 
   Size:        898      74     617    1216     758     577     496    2017

  Fragments arranged by size: 

                2017    1216     898     758     617     577     496      74
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1352    1352 
   Size:       6653
 
BsiEI CG_ry'CG

Cuts at:    104    4195    4241    5449     104 
   Size:       4091      46    1208    1308

  Fragments arranged by size: 

                4091    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    195    1539    2645    2913    3667    4445    4635    5427     195 
   Size:       1344    1106     268     754     778     190     792    1421

  Fragments arranged by size: 

                1421    1344    1106     792     778     754     268     190
            
 
BsmI GAATG_Cn'

Cuts at:    983    1753     983 
   Size:        770    5883
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1929    1929 
   Size:       6653
 
Bsp1286I G_dGCh'C

Cuts at:    161     195    1539    2645    2810    2863    2913    3667    4278 
   Size:         34    1344    1106     165      53      50     754     611

Cuts at:   4278    4371    4445    4635    4697    5427     161 
   Size:         93      74     190      62     730    1387

  Fragments arranged by size: 

                1387    1344    1106     754     730     611     190     165
                  93      74      62      53      50      34
 
BspEI T'CCGG_A

Cuts at:   5873    5873 
   Size:       6653
 
BspHI T'CATG_A

Cuts at:    431    1542    5829     431 
   Size:       1111    4287    1255

  Fragments arranged by size: 

                4287    1255    1111
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1434    3207    3703    3917    4219    4600    1434 
   Size:       1773     496     214     302     381    3487

  Fragments arranged by size: 

                3487    1773     496     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    4945    4999    6483    6648      50 
   Size:       4895      54    1484     165      55

  Fragments arranged by size: 

                4895    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    882     926    1353    1448    2117    4565     882 
   Size:         44     427      95     669    2448    2970

  Fragments arranged by size: 

                2970    2448     669     427      95      44
 
BsrFI r'CCGG_y

Cuts at:   3212    3585    4651    4832    5041    3212 
   Size:        373    1066     181     209    4824

  Fragments arranged by size: 

                4824    1066     373     209     181
 
BsrGI T'GTAC_A

Cuts at:    475     475 
   Size:       6653
 
BssSI C'ACGA_G

Cuts at:   4924    5282    4924 
   Size:        358    6295
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1081    4030    1081 
   Size:       2949    3704
 
BstBI TT'CG_AA

Cuts at:   2369    5014    2369 
   Size:       2645    4008
 
BstEII G'GTnAC_C

Cuts at:    390     390 
   Size:       6653
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2129    2307    2419    2986    2129 
   Size:        178     112     567    5796

  Fragments arranged by size: 

                5796     567     178     112
 
BstYI r'GATC_y

Cuts at:      1    2756    2967    3678    4145    4168    4503    4749    5761 
   Size:       2755     211     711     467      23     335     246    1012

Cuts at:   5761    5847    5859    6205       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                2755    1012     711     467     449     346     335     246
                 211      86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2075    3217    4603    4784    4947    2075 
   Size:       1142    1386     181     163    3781

  Fragments arranged by size: 

                3781    1386    1142     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2225    3896    2225 
   Size:       1671    4982
 
ClaI AT'CG_AT

Cuts at:    374     374 
   Size:       6653
 
DraI TTT'AAA

Cuts at:   1518    3095    3104    3652    1518 
   Size:       1577       9     548    4519

  Fragments arranged by size: 

                4519    1577     548       9
 
DraIII CAC_nnn'GTG

Cuts at:    679     679 
   Size:       6653
 
DrdI GACnn_nn'nnGTC

Cuts at:    388    4359    5217     388 
   Size:       3971     858    1824

  Fragments arranged by size: 

                3971    1824     858
 
EaeI y'GGCC_r

Cuts at:   1901    3525    4238    4412    4803    4830    6175    1901 
   Size:       1624     713     174     391      27    1345    2379

  Fragments arranged by size: 

                2379    1624    1345     713     391     174      27
 
EagI C'GGCC_G

Cuts at:   4238    4238 
   Size:       6653
 
EarI CTCTTCn'nnn_

Cuts at:    175    1544    2230    4676    4886     175 
   Size:       1369     686    2446     210    1942

  Fragments arranged by size: 

                2446    1942    1369     686     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    514    1917    4861    5171    5317    6463     514 
   Size:       1403    2944     310     146    1146     704

  Fragments arranged by size: 

                2944    1403    1146     704     310     146
 
EcoICRI GAG'CTC

Cuts at:   3665    3665 
   Size:       6653
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    851     940    1816    1952    2165    2374    2591    2876    3443 
   Size:         89     876     136     213     209     217     285     567

Cuts at:   3443    3611    4478    4910    5657    5925     851 
   Size:        168     867     432     747     268    1579

  Fragments arranged by size: 

                1579     876     867     747     567     432     285     268
                 217     213     209     168     136      89
 
EcoNI CCTnn'n_nnAGG

Cuts at:    407     733     407 
   Size:        326    6327
 
EcoO109I rG'GnC_Cy

Cuts at:    708    1184    1886    2517    2649    2859    3046    3792     708 
   Size:        476     702     631     132     210     187     746    3569

  Fragments arranged by size: 

                3569     746     702     631     476     210     187     132
            
 
EcoRI G'AATT_C

Cuts at:   3636    3657    3636 
   Size:         21    6632
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    156     223     577    4672     156 
   Size:         67     354    4095    2137

  Fragments arranged by size: 

                4095    2137     354      67
 
FspI TGC'GCA

Cuts at:   4434    4434 
   Size:       6653
 
HaeII r_GCGC'y

Cuts at:    141    1937    2412    4087    4335    5357    6336     141 
   Size:       1796     475    1675     248    1022     979     458

  Fragments arranged by size: 

                1796    1675    1022     979     475     458     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1909    1941    2919    2951    4495    4525    4527    4557    1909 
   Size:         32     978      32    1544      30       2      30    4005

  Fragments arranged by size: 

                4005    1544     978      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1235    1839    3504    3627    3692    1235 
   Size:        604    1665     123      65    4196

  Fragments arranged by size: 

                4196    1665     604     123      65
 
HindIII A'AGCT_T

Cuts at:    336     336 
   Size:       6653
 
HpaI GTT'AAC

Cuts at:   1235    1235 
   Size:       6653
 
KasI G'GCGC_C

Cuts at:   2408    4083    4331    2408 
   Size:       1675     248    4730

  Fragments arranged by size: 

                4730    1675     248
 
KpnI G_GTAC'C

Cuts at:   3673    3673 
   Size:       6653
 
MfeI C'AATT_G

Cuts at:    617     617 
   Size:       6653
 
MluI A'CGCG_T

Cuts at:   6086    6086 
   Size:       6653
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    268    1987    2962    5324    5508     268 
   Size:       1719     975    2362     184    1413

  Fragments arranged by size: 

                2362    1719    1413     975     184
 
MscI TGG'CCA

Cuts at:   1903    3527    4414    1903 
   Size:       1624     887    4142

  Fragments arranged by size: 

                4142    1624     887
 
MslI CAynn'nnrTG

Cuts at:    543     921    1568    1897    2305    2984    4642    4769     543 
   Size:        378     647     329     408     679    1658     127    2427

  Fragments arranged by size: 

                2427    1658     679     647     408     378     329     127
            
 
MspA1I CmG'CkG

Cuts at:   1883    2043    2175    2305    2551    3764    4078    4438    5451 
   Size:        160     132     130     246    1213     314     360    1013

Cuts at:   5451    5696    1883 
   Size:        245    2840

  Fragments arranged by size: 

                2840    1213    1013     360     314     246     245     160
                 132     130
 
NaeI GCC'GGC

Cuts at:   3214    3587    4834    3214 
   Size:        373    1247    5033

  Fragments arranged by size: 

                5033    1247     373
 
NarI GG'CG_CC

Cuts at:   2409    4084    4332    2409 
   Size:       1675     248    4730

  Fragments arranged by size: 

                4730    1675     248
 
NgoMIV G'CCGG_C

Cuts at:   3212    3585    4832    3212 
   Size:        373    1247    5033

  Fragments arranged by size: 

                5033    1247     373
 
NsiI A_TGCA'T

Cuts at:   1086    1086 
   Size:       6653
 
NspI r_CATG'y

Cuts at:   2793    3706    4206    5113    2793 
   Size:        913     500     907    4333

  Fragments arranged by size: 

                4333     913     907     500
 
PciI A'CATG_T

Cuts at:   5109    5109 
   Size:       6653
 
PflMI CCAn_nnn'nTGG

Cuts at:   1511    1511 
   Size:       6653
 
PfoI T'CCnGG_A

Cuts at:    343     343 
   Size:       6653
 
PmeI GTTT'AAAC

Cuts at:   3652    3652 
   Size:       6653
 
PmlI CAC'GTG

Cuts at:   1895    1895 
   Size:       6653
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5792    5824    5792 
   Size:         32    6621
 
PpuMI rG'GwC_Cy

Cuts at:    708    1184    1886    2649     708 
   Size:        476     702     763    4712

  Fragments arranged by size: 

                4712     763     702     476
 
PsiI TTA'TAA

Cuts at:   1004    1004 
   Size:       6653
 
PspOMI G'GGCC_C

Cuts at:   2859    2859 
   Size:       6653
 
PstI C_TGCA'G

Cuts at:    504     610    1198    3700    4385     504 
   Size:        106     588    2502     685    2772

  Fragments arranged by size: 

                2772    2502     685     588     106
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6653
 
PvuII CAG'CTG

Cuts at:   2043    2175    2305    2551    4078    4438    2043 
   Size:        132     130     246    1527     360    4258

  Fragments arranged by size: 

                4258    1527     360     246     132     130
 
RsrII CG'GwC_CG

Cuts at:   4848    4848 
   Size:       6653
 
SacI G_AGCT'C

Cuts at:   3667    3667 
   Size:       6653
 
SalI G'TCGA_C

Cuts at:   3690    3690 
   Size:       6653
 
SanDI GG'GwC_CC

Cuts at:   2649    2649 
   Size:       6653
 
SapI GCTCTTCn'nnn_

Cuts at:   1544    2230    4676    4886    1544 
   Size:        686    2446     210    3311

  Fragments arranged by size: 

                3311    2446     686     210
 
SbfI CC_TGCA'GG

Cuts at:   3700    3700 
   Size:       6653
 
ScaI AGT'ACT

Cuts at:    599    6170     599 
   Size:       5571    1082
 
SexAI A'CCwGG_T

Cuts at:   1442    1442 
   Size:       6653
 
SfcI C'TryA_G

Cuts at:     33     500     606    1194    2552    3696    4381    5374    5565 
   Size:        467     106     588    1358    1144     685     993     191

Cuts at:   5565      33 
   Size:       1121

  Fragments arranged by size: 

                1358    1144    1121     993     685     588     467     191
                 106
 
SfoI GGC'GCC

Cuts at:   2410    4085    4333    2410 
   Size:       1675     248    4730

  Fragments arranged by size: 

                4730    1675     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6653
 
SmaI CCC'GGG

Cuts at:   3675    3675 
   Size:       6653
 
SmlI C'TyrA_G

Cuts at:    692    3808    4723    5215    5477     692 
   Size:       3116     915     492     262    1868

  Fragments arranged by size: 

                3116    1868     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6653
 
SphI G_CATG'C

Cuts at:   3706    4206    3706 
   Size:        500    6153
 
SspI AAT'ATT

Cuts at:    297     641     297 
   Size:        344    6309
 
StuI AGG'CCT

Cuts at:   1461    2387    1461 
   Size:        926    5727
 
StyI C'CwwG_G

Cuts at:   2769    3787    4141    2769 
   Size:       1018     354    5281

  Fragments arranged by size: 

                5281    1018     354
 
SwaI ATTT'AAAT

Cuts at:   3104    3104 
   Size:       6653
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    268    4236    4369    4828    5037     268 
   Size:       3968     133     459     209    1884

  Fragments arranged by size: 

                3968    1884     459     209     133
 
TatI w'GTAC_w

Cuts at:    475     597    1606    2393    3002    6168     475 
   Size:        122    1009     787     609    3166     960

  Fragments arranged by size: 

                3166    1009     960     787     609     122
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1400    1432    1759    1791    2592    2624    2816    2848    6170 
   Size:         32     327      32     801      32     192      32    3322

Cuts at:   6170    6202    1400 
   Size:         32    1851

  Fragments arranged by size: 

                3322    1851     801     327     192      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1921    2878    4450    1921 
   Size:        957    1572    4124

  Fragments arranged by size: 

                4124    1572     957
 
XbaI T'CTAG_A

Cuts at:   3684    3684 
   Size:       6653
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    341     956    1508    1911    4148     341 
   Size:        615     552     403    2237    2846

  Fragments arranged by size: 

                2846    2237     615     552     403
 
XmaI C'CCGG_G

Cuts at:   3673    3673 
   Size:       6653
 
XmnI GAAnn'nnTTC

Cuts at:   2365    3456    2365 
   Size:       1091    5562
 
ZraI GAC'GTC

Cuts at:   2740    2740 
   Size:       6653
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI     NspI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AhdI     AlfI     AloI     AscI    AvrII     BaeI    BbvCI
     BglI    BmgBI     BmtI    BsiWI   BssHII  BstZ17I   Bsu36I     BtgI
    CspCI    EcoRV     FseI    FspAI     NcoI     NdeI     NheI     NotI
     NruI     PacI     PasI    PshAI    PspXI     PsrI    SacII     SfiI
    SgrAI    SnaBI     SrfI     XhoI
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