Restriction map for pF1KA0338
 (Linear) (Six-base) MAPSORT of: KIAA0338.seq  Check: 2252  from: 1  to: 2643



KIAA0338.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AccI GT'mk_AC

Cuts at:      0     854    2588    2643 
   Size:        854    1734      55

  Fragments arranged by size: 

                1734     854      55
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1534    1573    1801    2643 
   Size:       1534      39     228     842

  Fragments arranged by size: 

                1534     842     228      39
 
AgeI A'CCGG_T

Cuts at:      0    1070    2643 
   Size:       1070    1573
 
AleI CACnn'nnGTG

Cuts at:      0      91    2643 
   Size:         91    2552
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1759    1791    2643 
   Size:       1759      32     852

  Fragments arranged by size: 

                1759     852      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     363    1348    1402    2523    2643 
   Size:        363     985      54    1121     120

  Fragments arranged by size: 

                1121     985     363     120      54
 
ApaI G_GGCC'C

Cuts at:      0    1725    1995    2643 
   Size:       1725     270     648

  Fragments arranged by size: 

                1725     648     270
 
ApoI r'AATT_y

Cuts at:      0     501    2643 
   Size:        501    2142
 
AvaI C'yCGr_G

Cuts at:      0      64     740     795    1718    1973    2643 
   Size:         64     676      55     923     255     670

  Fragments arranged by size: 

                 923     676     670     255      64      55
 
BanI G'GyrC_C

Cuts at:      0    1645    2389    2643 
   Size:       1645     744     254

  Fragments arranged by size: 

                1645     744     254
 
BanII G_rGCy'C

Cuts at:      0     649     719    1142    1171    1541    1562    1690    1725 
   Size:        649      70     423      29     370      21     128      35

Cuts at:   1725    1928    1995    2081    2643 
   Size:        203      67      86     562

  Fragments arranged by size: 

                 649     562     423     370     203     128      86      70
                  67      35      29      21
 
BbeI G_GCGC'C

Cuts at:      0    2393    2643 
   Size:       2393     250
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     560    1907    2643 
   Size:        560    1347     736

  Fragments arranged by size: 

                1347     736     560
 
BbvCI CC'TCA_GC

Cuts at:      0     686    1403    1678    1853    2643 
   Size:        686     717     275     175     790

  Fragments arranged by size: 

                 790     717     686     275     175
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      55      89    1878    1912    2030    2064    2643 
   Size:         55      34    1789      34     118      34     579

  Fragments arranged by size: 

                1789     579     118      55      34      34      34
 
BglII A'GATC_T

Cuts at:      0     977    2643 
   Size:        977    1666
 
BlpI GC'TnA_GC

Cuts at:      0     665    2643 
   Size:        665    1978
 
Bme1580I G_kGCm'C

Cuts at:      0    1648    1725    1995    2643 
   Size:       1648      77     270     648

  Fragments arranged by size: 

                1648     648     270      77
 
BmgBI CAC'GTC

Cuts at:      0    1714    2643 
   Size:       1714     929
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     652    1602    2064    2501    2643 
   Size:        652     950     462     437     142

  Fragments arranged by size: 

                 950     652     462     437     142
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     144    2643 
   Size:        144    2499
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     769    1800    1867    2643 
   Size:        769    1031      67     776

  Fragments arranged by size: 

                1031     776     769      67
 
Bpu10I CC'TnA_GC

Cuts at:      0      41     686    1136    1403    1678    1853    1922    2643 
   Size:         41     645     450     267     275     175      69     721

  Fragments arranged by size: 

                 721     645     450     275     267     175      69      41
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     958    2263    2643 
   Size:        958    1305     380

  Fragments arranged by size: 

                1305     958     380
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2242    2643 
   Size:       2242     401
 
BsaBI GATnn'nnATC

Cuts at:      0    2507    2643 
   Size:       2507     136
 
BsaHI Gr'CG_yC

Cuts at:      0    2390    2643 
   Size:       2390     253
 
BsaWI w'CCGG_w

Cuts at:      0     485    1070    1178    2643 
   Size:        485     585     108    1465

  Fragments arranged by size: 

                1465     585     485     108
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     313     343    2643 
   Size:        313      30    2300

  Fragments arranged by size: 

                2300     313      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     205     703     766    1005    1420    1608    1708 
   Size:         61     144     498      63     239     415     188     100

Cuts at:   1708    2629    2643 
   Size:        921      14

  Fragments arranged by size: 

                 921     498     415     239     188     144     100      63
                  61      14
 
BseYI C'CCAG_C

Cuts at:      0     149     238     542     669    1314    1996    2180    2643 
   Size:        149      89     304     127     645     682     184     463

  Fragments arranged by size: 

                 682     645     463     304     184     149     127      89
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     679     844    1223    2643 
   Size:        679     165     379    1420

  Fragments arranged by size: 

                1420     679     379     165
 
BsiEI CG_ry'CG

Cuts at:      0    1932    2643 
   Size:       1932     711
 
BsiHKAI G_wGCw'C

Cuts at:      0     719    1045    1541    1816    1928    2643 
   Size:        719     326     496     275     112     715

  Fragments arranged by size: 

                 719     715     496     326     275     112
 
BsmI GAATG_Cn'

Cuts at:      0     831    2643 
   Size:        831    1812
 
Bsp1286I G_dGCh'C

Cuts at:      0     649     719    1045    1142    1171    1541    1562    1648 
   Size:        649      70     326      97      29     370      21      86

Cuts at:   1648    1690    1725    1816    1928    1995    2081    2643 
   Size:         42      35      91     112      67      86     562

  Fragments arranged by size: 

                 649     562     370     326     112      97      91      86
                  86      70      67      42      35      29      21
 
BspEI T'CCGG_A

Cuts at:      0     485    2643 
   Size:        485    2158
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     866    2175    2643 
   Size:        866    1309     468

  Fragments arranged by size: 

                1309     866     468
 
BsrFI r'CCGG_y

Cuts at:      0     608     935    1070    1232    2643 
   Size:        608     327     135     162    1411

  Fragments arranged by size: 

                1411     608     327     162     135
 
BstBI TT'CG_AA

Cuts at:      0    1898    2643 
   Size:       1898     745
 
BstEII G'GTnAC_C

Cuts at:      0    2571    2643 
   Size:       2571      72
 
BstYI r'GATC_y

Cuts at:      0     482     968     977    1010    1977    2643 
   Size:        482     486       9      33     967     666

  Fragments arranged by size: 

                 967     666     486     482      33       9
 
BstZ17I GTA'TAC

Cuts at:      0    2589    2643 
   Size:       2589      54
 
BtgI C'CryG_G

Cuts at:      0     108    1884    2243    2264    2330    2643 
   Size:        108    1776     359      21      66     313

  Fragments arranged by size: 

                1776     359     313     108      66      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1757    2643 
   Size:       1757     886
 
BtsI GCAGTG_nn'

Cuts at:      0    2393    2643 
   Size:       2393     250
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1867    2643 
   Size:       1867     776
 
EaeI y'GGCC_r

Cuts at:      0     111     348    1796    2114    2327    2531    2643 
   Size:        111     237    1448     318     213     204     112

  Fragments arranged by size: 

                1448     318     237     213     204     112     111
 
EcoICRI GAG'CTC

Cuts at:      0     717    1539    1926    2643 
   Size:        717     822     387     717

  Fragments arranged by size: 

                 822     717     717     387
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     769    1534    1573    1800    1801    1867    2643 
   Size:        769     765      39     227       1      66     776

  Fragments arranged by size: 

                 776     769     765     227      66      39       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     400     987    2643 
   Size:        400     587    1656

  Fragments arranged by size: 

                1656     587     400
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16      51    1193    1345    1547    1722    1959    1991 
   Size:         16      35    1142     152     202     175     237      32

Cuts at:   1991    1992    2045    2520    2643 
   Size:          1      53     475     123

  Fragments arranged by size: 

                1142     475     237     202     175     152     123      53
                  35      32      16       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     426    2643 
   Size:        426    2217
 
HaeII r_GCGC'y

Cuts at:      0    1223    1895    2393    2643 
   Size:       1223     672     498     250

  Fragments arranged by size: 

                1223     672     498     250
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1363    1395    1996    2028    2239    2271    2643 
   Size:       1363      32     601      32     211      32     372

  Fragments arranged by size: 

                1363     601     372     211      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     835    2643 
   Size:        835    1808
 
KasI G'GCGC_C

Cuts at:      0    2389    2643 
   Size:       2389     254
 
MfeI C'AATT_G

Cuts at:      0    1046    2643 
   Size:       1046    1597
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1450    2643 
   Size:       1450    1193
 
MscI TGG'CCA

Cuts at:      0    1798    2116    2329    2533    2643 
   Size:       1798     318     213     204     110

  Fragments arranged by size: 

                1798     318     213     204     110
 
MslI CAynn'nnrTG

Cuts at:      0      91    2643 
   Size:         91    2552
 
MspA1I CmG'CkG

Cuts at:      0      79     546     585    2299    2643 
   Size:         79     467      39    1714     344

  Fragments arranged by size: 

                1714     467     344      79      39
 
NaeI GCC'GGC

Cuts at:      0     610    2643 
   Size:        610    2033
 
NarI GG'CG_CC

Cuts at:      0    2390    2643 
   Size:       2390     253
 
NcoI C'CATG_G

Cuts at:      0    1884    2264    2643 
   Size:       1884     380     379

  Fragments arranged by size: 

                1884     380     379
 
NgoMIV G'CCGG_C

Cuts at:      0     608    2643 
   Size:        608    2035
 
NspI r_CATG'y

Cuts at:      0     637     697    2643 
   Size:        637      60    1946

  Fragments arranged by size: 

                1946     637      60
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2523    2643 
   Size:       2523     120
 
PfoI T'CCnGG_A

Cuts at:      0    2284    2643 
   Size:       2284     359
 
PpuMI rG'GwC_Cy

Cuts at:      0    1193    1547    2643 
   Size:       1193     354    1096

  Fragments arranged by size: 

                1193    1096     354
 
PshAI GACnn'nnGTC

Cuts at:      0    2158    2643 
   Size:       2158     485
 
PspOMI G'GGCC_C

Cuts at:      0    1721    1991    2643 
   Size:       1721     270     652

  Fragments arranged by size: 

                1721     652     270
 
PstI C_TGCA'G

Cuts at:      0     105     584    2153    2555    2643 
   Size:        105     479    1569     402      88

  Fragments arranged by size: 

                1569     479     402     105      88
 
PvuII CAG'CTG

Cuts at:      0     546     585    2299    2643 
   Size:        546      39    1714     344

  Fragments arranged by size: 

                1714     546     344      39
 
SacI G_AGCT'C

Cuts at:      0     719    1541    1928    2643 
   Size:        719     822     387     715

  Fragments arranged by size: 

                 822     719     715     387
 
SbfI CC_TGCA'GG

Cuts at:      0     105    2643 
   Size:        105    2538
 
SexAI A'CCwGG_T

Cuts at:      0     371    2643 
   Size:        371    2272
 
SfcI C'TryA_G

Cuts at:      0     101     201     580    2149    2551    2643 
   Size:        101     100     379    1569     402      92

  Fragments arranged by size: 

                1569     402     379     101     100      92
 
SfoI GGC'GCC

Cuts at:      0    2391    2643 
   Size:       2391     252
 
SmaI CCC'GGG

Cuts at:      0     742     797    1720    1975    2643 
   Size:        742      55     923     255     668

  Fragments arranged by size: 

                 923     742     668     255      55
 
SmlI C'TyrA_G

Cuts at:      0     973    2278    2643 
   Size:        973    1305     365

  Fragments arranged by size: 

                1305     973     365
 
SphI G_CATG'C

Cuts at:      0     637    2643 
   Size:        637    2006
 
StyI C'CwwG_G

Cuts at:      0     464     869    1148    1640    1884    2264    2643 
   Size:        464     405     279     492     244     380     379

  Fragments arranged by size: 

                 492     464     405     380     379     279     244
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     131     661    1161    1311    2194    2440    2643 
   Size:        131     530     500     150     883     246     203

  Fragments arranged by size: 

                 883     530     500     246     203     150     131
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     313     345    2643 
   Size:        313      32    2298

  Fragments arranged by size: 

                2298     313      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     373    2643 
   Size:        373    2270
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1155    2188    2643 
   Size:       1155    1033     455

  Fragments arranged by size: 

                1155    1033     455
 
XmaI C'CCGG_G

Cuts at:      0     740     795    1718    1973    2643 
   Size:        740      55     923     255     670

  Fragments arranged by size: 

                 923     740     670     255      55
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     AleI     AloI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrFI    BstBI
   BstEII    BstYI  BstZ17I     BtgI    BtgZI     BtsI     DrdI     EaeI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I  HindIII
     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI    PflMI     PfoI    PpuMI    PshAI   PspOMI
     PstI    PvuII     SacI     SbfI    SexAI     SfcI     SfoI     SmaI
     SmlI     SphI     StyI    TaqII     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BciVI     BclI    BfrBI     BglI     BmtI    BsaAI    BsiWI    BsmBI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstXI
   Bsu36I     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KpnI
     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     ScaI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TatI
     XbaI     XhoI     XmnI     ZraI
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