Restriction map for pF1KA0333
##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AccI        AclI        AflII       AflIII      AgeI        
ApaI        AscI        AseI        AsnI        AspI        
AviII       AvrII       BbvCI       BcgI        BclI        
BfrI        BlnI        BsiWI       BsmBI       BsmI        
BspDI       BspLU11I    BssHII      Bst1107I    BstXI       
BstZ17I     ClaI        DraI        DrdI        EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NsiI        PacI        PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
SalI        SbfI        ScaI        SfiI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        2491  GACGTC               GACGT^C
Acc65I       1926  GGTACC               G^GTACC
AccIII       2685  TCCGGA               T^CCGGA
AhdI         1600  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1396  GTGCAC               G^TGCAC
Aor51HI       349  AGCGCT               AGC^GCT
ApaLI        1396  GTGCAC               G^TGCAC
Asp700I       868  GAATGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1926  GGTACC               G^GTACC
AspEI        1600  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         2805  TGGCCA               TGG^CCA
BamHI         548  GGATCC               G^GATCC
BsaBI        2803  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2685  TCCGGA               [AT]^CCGG[AT]
BseAI        2685  TCCGGA               T^CCGGA
Bsp1407I     1048  TGTACA               T^GTACA
BspEI        2685  TCCGGA               T^CCGGA
BspHI        2078  TCATGA               T^CATGA
BsrGI        1048  TGTACA               T^GTACA
BssSI        1696  CTCGTG               CACGAG(-5/-1)
BstBI          86  TTCGAA               TT^CGAA
BstEII       2871  GGTCACC              G^GT[ACGT]ACC
BstPI        2871  GGTCACC              G^GT[ACGT]ACC
CpoI         1842  CGGACCG              CG^G[AT]CCG
DraIII       2968  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2892  CGGCCG               C^GGCCG
Eam1105I     1600  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2892  CGGCCG               C^GGCCG
Eco47III      349  AGCGCT               AGC^GCT
Eco52I       2892  CGGCCG               C^GGCCG
EcoNI        3246  CCTCAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2871  GGTCACC              G^GT[ACGT]ACC
KpnI         1926  GGTACC               GGTAC^C
MamI         2803  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2805  TGGCCA               TGG^CCA
MroI         2685  TCCGGA               T^CCGGA
MscI         2805  TGGCCA               TGG^CCA
NaeI         3548  GCCGGC               GCC^GGC
NgoMIV       3548  GCCGGC               G^CCGGC
NspV           86  TTCGAA               TT^CGAA
PaeR7I        905  CTCGAG               C^TCGAG
RcaI         2078  TCATGA               T^CATGA
RsrII        1842  CGGACCG              CG^G[AT]CCG
SacI         3346  GAGCTC               GAGCT^C
SapI         2469  GAAGAGC              GCTCTTC(1/4)
SexAI        3537  ACCAGGT              A^CC[AT]GGT
SfuI           86  TTCGAA               TT^CGAA
SmaI         1198  CCCGGG               CCC^GGG
SphI          263  GCATGC               GCATG^C
SspBI        1048  TGTACA               T^GTACA
StuI         2742  AGGCCT               AGG^CCT
XhoI          905  CTCGAG               C^TCGAG
XmaCI        1198  CCCGGG               C^CCGGG
XmaI         1198  CCCGGG               C^CCGGG
XmnI          868  GAATGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AocI           37  CCTAAGG              CC^T[ACGT]AGG
             1760  CCTCAGG             
BbeI          245  GGCGCC               GGCGC^C
             1170  GGCGCC              
BciVI        1250  GTATCC               GTATCC(6/5)
             3219  GTATCC              
BglI         1831  GCCAGGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1840  GCCGGACCGGC         
BglII        1148  AGATCT               A^GATCT
             2326  AGATCT              
BlpI         3040  GCTAAGC              GC^T[ACGT]AGC
             3489  GCTGAGC             
Bpu1102I     3040  GCTAAGC              GC^T[ACGT]AGC
             3489  GCTGAGC             
BsiEI         766  CGACCG               CG[GA][CT]^CG
             2892  CGGCCG              
BspMI        1851  ACCTGC               ACCTGC(4/8)
             1375  GCAGGT              
BsrBI        2909  CCGCTC               CCGCTC(-3/-3)
             2862  GAGCGG              
BsrDI         237  CATTGC               GCAATG(2/0)
               23  GCAATG              
BsrFI        1845  ACCGGC               [GA]^CCGG[CT]
             3548  GCCGGC              
BstAPI       1861  GCAACGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2918  GCAGTTCATGC         
Bsu36I         37  CCTAAGG              CC^T[ACGT]AGG
             1760  CCTCAGG             
CelII        3040  GCTAAGC              GC^T[ACGT]AGC
             3489  GCTGAGC             
Cfr10I       1845  ACCGGC               [GA]^CCGG[CT]
             3548  GCCGGC              
Eco81I         37  CCTAAGG              CC^T[ACGT]AGG
             1760  CCTCAGG             
KasI          245  GGCGCC               G^GCGCC
             1170  GGCGCC              
KspI          249  CCGCGG               CCGC^GG
             1664  CCGCGG              
NarI          245  GGCGCC               GG^CGCC
             1170  GGCGCC              
NcoI         1628  CCATGG               C^CATGG
             1792  CCATGG              
PstI         1236  CTGCAG               CTGCA^G
             1853  CTGCAG              
SacII         249  CCGCGG               CCGC^GG
             1664  CCGCGG              
SfcI         1236  CTGCAG               C^T[GA][CT]AG
             1853  CTGCAG              
SfoI          245  GGCGCC               GGC^GCC
             1170  GGCGCC              
XcmI          614  CCAGTGACACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2393  CCATCCCCGATCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbrPI       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PmaCI       
PmlI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
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