Restriction map for pF1KA0333
 (Linear) (Six-base) MAPSORT of: KIAA0333.seq  Check: 2735  from: 1  to: 3567



KIAA0333.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1860    3567 
   Size:       1860    1707
 
AatII G_ACGT'C

Cuts at:      0    2495    3567 
   Size:       2495    1072
 
Acc65I G'GTAC_C

Cuts at:      0    1926    3567 
   Size:       1926    1641
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475    1808    2215    2290    2481    2744    3089    3567 
   Size:        475    1333     407      75     191     263     345     478

  Fragments arranged by size: 

                1333     478     475     407     345     263     191      75
            
 
AfeI AGC'GCT

Cuts at:      0     351    3567 
   Size:        351    3216
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1605    3567 
   Size:       1605    1962
 
AleI CACnn'nnGTG

Cuts at:      0     391    2932    3278    3567 
   Size:        391    2541     346     289

  Fragments arranged by size: 

                2541     391     346     289
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     979    3567 
   Size:        979    2588
 
AlwNI CAG_nnn'CTG

Cuts at:      0     168     231    2629    3567 
   Size:        168      63    2398     938

  Fragments arranged by size: 

                2398     938     168      63
 
ApaLI G'TGCA_C

Cuts at:      0    1396    3567 
   Size:       1396    2171
 
ApoI r'AATT_y

Cuts at:      0    1998    2006    2561    3202    3567 
   Size:       1998       8     555     641     365

  Fragments arranged by size: 

                1998     641     555     365       8
 
AvaI C'yCGr_G

Cuts at:      0      18     655     905    1198    1474    2812    3567 
   Size:         18     637     250     293     276    1338     755

  Fragments arranged by size: 

                1338     755     637     293     276     250      18
 
BamHI G'GATC_C

Cuts at:      0     548    3567 
   Size:        548    3019
 
BanI G'GyrC_C

Cuts at:      0      33     245    1039    1170    1177    1774    1848    1926 
   Size:         33     212     794     131       7     597      74      78

Cuts at:   1926    3021    3567 
   Size:       1095     546

  Fragments arranged by size: 

                1095     794     597     546     212     131      78      74
                  33       7
 
BanII G_rGCy'C

Cuts at:      0     296     527     607     656    2819    3350    3567 
   Size:        296     231      80      49    2163     531     217

  Fragments arranged by size: 

                2163     531     296     231     217      80      49
 
BbeI G_GCGC'C

Cuts at:      0     249    1174    3567 
   Size:        249     925    2393

  Fragments arranged by size: 

                2393     925     249
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     941    1091    1345    1604    1703    2377    2487    2648 
   Size:        941     150     254     259      99     674     110     161

Cuts at:   2648    2837    3304    3323    3567 
   Size:        189     467      19     244

  Fragments arranged by size: 

                 941     674     467     259     254     244     189     161
                 150     110      99      19
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1261    3230    3567 
   Size:       1261    1969     337

  Fragments arranged by size: 

                1969    1261     337
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1837    1846    3567 
   Size:       1837       9    1721

  Fragments arranged by size: 

                1837    1721       9
 
BglII A'GATC_T

Cuts at:      0    1148    2326    3567 
   Size:       1148    1178    1241

  Fragments arranged by size: 

                1241    1178    1148
 
BlpI GC'TnA_GC

Cuts at:      0    3041    3490    3567 
   Size:       3041     449      77

  Fragments arranged by size: 

                3041     449      77
 
Bme1580I G_kGCm'C

Cuts at:      0    1400    1777    3026    3567 
   Size:       1400     377    1249     541

  Fragments arranged by size: 

                1400    1249     541     377
 
BmgBI CAC'GTC

Cuts at:      0     299    3465    3567 
   Size:        299    3166     102

  Fragments arranged by size: 

                3166     299     102
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     369     635    1343    3567 
   Size:        369     266     708    2224

  Fragments arranged by size: 

                2224     708     369     266
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     345    1427    2262    3567 
   Size:        345    1082     835    1305

  Fragments arranged by size: 

                1305    1082     835     345
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     298     773    1046    1397    1640    2425    2780 
   Size:        163     135     475     273     351     243     785     355

Cuts at:   2780    3107    3567 
   Size:        327     460

  Fragments arranged by size: 

                 785     475     460     355     351     327     273     243
                 163     135
 
Bpu10I CC'TnA_GC

Cuts at:      0     728    1290    1370    3567 
   Size:        728     562      80    2197

  Fragments arranged by size: 

                2197     728     562      80
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1129    3310    3567 
   Size:       1129    2181     257

  Fragments arranged by size: 

                2181    1129     257
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     352    1014    1398    1420    3567 
   Size:        352     662     384      22    2147

  Fragments arranged by size: 

                2147     662     384     352      22
 
BsaAI yAC'GTr

Cuts at:      0    1212    2306    2455    3567 
   Size:       1212    1094     149    1112

  Fragments arranged by size: 

                1212    1112    1094     149
 
BsaBI GATnn'nnATC

Cuts at:      0    2807    3567 
   Size:       2807     760
 
BsaHI Gr'CG_yC

Cuts at:      0     246     317     632    1171    1962    2492    3567 
   Size:        246      71     315     539     791     530    1075

  Fragments arranged by size: 

                1075     791     539     530     315     246      71
 
BsaWI w'CCGG_w

Cuts at:      0    2685    3567 
   Size:       2685     882
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1545    1575    1638    1668    3567 
   Size:       1545      30      63      30    1899

  Fragments arranged by size: 

                1899    1545      63      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     273     595     760    1627    3400    3567 
   Size:         43     230     322     165     867    1773     167

  Fragments arranged by size: 

                1773     867     322     230     167     165      43
 
BseYI C'CCAG_C

Cuts at:      0     168     685    2679    2847    3567 
   Size:        168     517    1994     168     720

  Fragments arranged by size: 

                1994     720     517     168     168
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     939     991    1667    1967    2338    2654    3567 
   Size:        939      52     676     300     371     316     913

  Fragments arranged by size: 

                 939     913     676     371     316     300      52
 
BsiEI CG_ry'CG

Cuts at:      0     769    2895    3567 
   Size:        769    2126     672

  Fragments arranged by size: 

                2126     769     672
 
BsiHKAI G_wGCw'C

Cuts at:      0    1371    1400    2476    3350    3567 
   Size:       1371      29    1076     874     217

  Fragments arranged by size: 

                1371    1076     874     217      29
 
Bsp1286I G_dGCh'C

Cuts at:      0     296     527     607     656    1371    1400    1777    2476 
   Size:        296     231      80      49     715      29     377     699

Cuts at:   2476    2819    3026    3350    3567 
   Size:        343     207     324     217

  Fragments arranged by size: 

                 715     699     377     343     324     296     231     217
                 207      80      49      29
 
BspEI T'CCGG_A

Cuts at:      0    2685    3567 
   Size:       2685     882
 
BspHI T'CATG_A

Cuts at:      0    2078    3567 
   Size:       2078    1489
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1366    1860    3567 
   Size:       1366     494    1707

  Fragments arranged by size: 

                1707    1366     494
 
BsrBI CCG'CTC

Cuts at:      0    2864    2911    3567 
   Size:       2864      47     656

  Fragments arranged by size: 

                2864     656      47
 
BsrDI GCAATG_nn'

Cuts at:      0      30     236    3567 
   Size:         30     206    3331

  Fragments arranged by size: 

                3331     206      30
 
BsrFI r'CCGG_y

Cuts at:      0    1845    3548    3567 
   Size:       1845    1703      19

  Fragments arranged by size: 

                1845    1703      19
 
BsrGI T'GTAC_A

Cuts at:      0    1048    3567 
   Size:       1048    2519
 
BssSI C'ACGA_G

Cuts at:      0    1696    3567 
   Size:       1696    1871
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1867    2924    3567 
   Size:       1867    1057     643

  Fragments arranged by size: 

                1867    1057     643
 
BstBI TT'CG_AA

Cuts at:      0      87    3567 
   Size:         87    3480
 
BstEII G'GTnAC_C

Cuts at:      0    2871    3567 
   Size:       2871     696
 
BstYI r'GATC_y

Cuts at:      0     548    1148    2326    3567 
   Size:        548     600    1178    1241

  Fragments arranged by size: 

                1241    1178     600     548
 
Bsu36I CC'TnA_GG

Cuts at:      0      38    1761    3567 
   Size:         38    1723    1806

  Fragments arranged by size: 

                1806    1723      38
 
BtgI C'CryG_G

Cuts at:      0     249    1628    1664    1792    2933    3426    3567 
   Size:        249    1379      36     128    1141     493     141

  Fragments arranged by size: 

                1379    1141     493     249     141     128      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     482    1973    2081    3567 
   Size:        482    1491     108    1486

  Fragments arranged by size: 

                1491    1486     482     108
 
BtsI GCAGTG_nn'

Cuts at:      0     195    1451    3567 
   Size:        195    1256    2116

  Fragments arranged by size: 

                2116    1256     195
 
DraIII CAC_nnn'GTG

Cuts at:      0    2973    3567 
   Size:       2973     594
 
EaeI y'GGCC_r

Cuts at:      0    2805    2892    3429    3567 
   Size:       2805      87     537     138

  Fragments arranged by size: 

                2805     537     138      87
 
EagI C'GGCC_G

Cuts at:      0    2892    3567 
   Size:       2892     675
 
EarI CTCTTCn'nnn_

Cuts at:      0     417    1395    2224    2464    2548    3377    3567 
   Size:        417     978     829     240      84     829     190

  Fragments arranged by size: 

                 978     829     829     417     240     190      84
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2405    3567 
   Size:       2405    1162
 
EcoICRI GAG'CTC

Cuts at:      0    3348    3567 
   Size:       3348     219
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     298     475     773    1046    1397    1640    1808 
   Size:        163     135     177     298     273     351     243     168

Cuts at:   1808    2215    2290    2425    2481    2744    2780    3089    3107 
   Size:        407      75     135      56     263      36     309      18

Cuts at:   3107    3567 
   Size:        460

  Fragments arranged by size: 

                 460     407     351     309     298     273     263     243
                 177     168     163     135     135      75      56      36
                  18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3250    3567 
   Size:       3250     317
 
EcoO109I rG'GnC_Cy

Cuts at:      0     536     586    2199    2898    3471    3501    3567 
   Size:        536      50    1613     699     573      30      66

  Fragments arranged by size: 

                1613     699     573     536      66      50      30
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3021    3567 
   Size:       3021     546
 
HaeII r_GCGC'y

Cuts at:      0     249     353     576    1174    2482    3497    3549    3567 
   Size:        249     104     223     598    1308    1015      52      18

  Fragments arranged by size: 

                1308    1015     598     249     223     104      52      18
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     283     315     436     468     540     572    1060    1092 
   Size:        283      32     121      32      72      32     488      32

Cuts at:   1092    1545    1577    1591    1623    2157    2189    2635    2667 
   Size:        453      32      14      32     534      32     446      32

Cuts at:   2667    3567 
   Size:        900

  Fragments arranged by size: 

                 900     534     488     453     446     283     121      72
                  32      32      32      32      32      32      32      32
                  14
 
KasI G'GCGC_C

Cuts at:      0     245    1170    3567 
   Size:        245     925    2397

  Fragments arranged by size: 

                2397     925     245
 
KpnI G_GTAC'C

Cuts at:      0    1930    3567 
   Size:       1930    1637
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     692    2405    3567 
   Size:        692    1713    1162

  Fragments arranged by size: 

                1713    1162     692
 
MscI TGG'CCA

Cuts at:      0    2807    3567 
   Size:       2807     760
 
MslI CAynn'nnrTG

Cuts at:      0     391    2765    2932    2968    3278    3567 
   Size:        391    2374     167      36     310     289

  Fragments arranged by size: 

                2374     391     310     289     167      36
 
MspA1I CmG'CkG

Cuts at:      0     168     251     391     860    1666    1828    1858    1894 
   Size:        168      83     140     469     806     162      30      36

Cuts at:   1894    2695    2826    3489    3567 
   Size:        801     131     663      78

  Fragments arranged by size: 

                 806     801     663     469     168     162     140     131
                  83      78      36      30
 
NaeI GCC'GGC

Cuts at:      0    3550    3567 
   Size:       3550      17
 
NarI GG'CG_CC

Cuts at:      0     246    1171    3567 
   Size:        246     925    2396

  Fragments arranged by size: 

                2396     925     246
 
NcoI C'CATG_G

Cuts at:      0    1628    1792    3567 
   Size:       1628     164    1775

  Fragments arranged by size: 

                1775    1628     164
 
NgoMIV G'CCGG_C

Cuts at:      0    3548    3567 
   Size:       3548      19
 
NspI r_CATG'y

Cuts at:      0     267    2508    3189    3567 
   Size:        267    2241     681     378

  Fragments arranged by size: 

                2241     681     378     267
 
PasI CC'CwG_GG

Cuts at:      0    2788    3567 
   Size:       2788     779
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1816    2803    2973    3567 
   Size:       1816     987     170     594

  Fragments arranged by size: 

                1816     987     594     170
 
PfoI T'CCnGG_A

Cuts at:      0    2796    3567 
   Size:       2796     771
 
PmlI CAC'GTG

Cuts at:      0    1212    2306    2455    3567 
   Size:       1212    1094     149    1112

  Fragments arranged by size: 

                1212    1112    1094     149
 
PpuMI rG'GwC_Cy

Cuts at:      0     586    2199    2898    3471    3501    3567 
   Size:        586    1613     699     573      30      66

  Fragments arranged by size: 

                1613     699     586     573      66      30
 
PspXI vC'TCGA_Gb

Cuts at:      0     905    3567 
   Size:        905    2662
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1919    1951    3567 
   Size:       1919      32    1616

  Fragments arranged by size: 

                1919    1616      32
 
PstI C_TGCA'G

Cuts at:      0    1240    1857    3567 
   Size:       1240     617    1710

  Fragments arranged by size: 

                1710    1240     617
 
PvuII CAG'CTG

Cuts at:      0     168     391    1828    2695    3567 
   Size:        168     223    1437     867     872

  Fragments arranged by size: 

                1437     872     867     223     168
 
RsrII CG'GwC_CG

Cuts at:      0    1843    3567 
   Size:       1843    1724
 
SacI G_AGCT'C

Cuts at:      0    3350    3567 
   Size:       3350     217
 
SacII CC_GC'GG

Cuts at:      0     252    1667    3567 
   Size:        252    1415    1900

  Fragments arranged by size: 

                1900    1415     252
 
SanDI GG'GwC_CC

Cuts at:      0     586    2898    3567 
   Size:        586    2312     669

  Fragments arranged by size: 

                2312     669     586
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2464    3567 
   Size:       2464    1103
 
SexAI A'CCwGG_T

Cuts at:      0    3537    3567 
   Size:       3537      30
 
SfcI C'TryA_G

Cuts at:      0    1236    1853    3567 
   Size:       1236     617    1714

  Fragments arranged by size: 

                1714    1236     617
 
SfoI GGC'GCC

Cuts at:      0     247    1172    3567 
   Size:        247     925    2395

  Fragments arranged by size: 

                2395     925     247
 
SmaI CCC'GGG

Cuts at:      0    1200    3567 
   Size:       1200    2367
 
SmlI C'TyrA_G

Cuts at:      0     905    1144    3325    3567 
   Size:        905     239    2181     242

  Fragments arranged by size: 

                2181     905     242     239
 
SphI G_CATG'C

Cuts at:      0     267    3567 
   Size:        267    3300
 
StuI AGG'CCT

Cuts at:      0    2744    3567 
   Size:       2744     823
 
StyI C'CwwG_G

Cuts at:      0    1089    1628    1792    1965    3567 
   Size:       1089     539     164     173    1602

  Fragments arranged by size: 

                1602    1089     539     173     164
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     783    2980    3221    3548    3567 
   Size:        783    2197     241     327      19

  Fragments arranged by size: 

                2197     783     327     241      19
 
TatI w'GTAC_w

Cuts at:      0    1048    1232    3567 
   Size:       1048     184    2335

  Fragments arranged by size: 

                2335    1048     184
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2444    2476    3567 
   Size:       2444      32    1091

  Fragments arranged by size: 

                2444    1091      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     621    2400    3567 
   Size:        621    1779    1167

  Fragments arranged by size: 

                1779    1167     621
 
XhoI C'TCGA_G

Cuts at:      0     905    3567 
   Size:        905    2662
 
XmaI C'CCGG_G

Cuts at:      0    1198    3567 
   Size:       1198    2369
 
XmnI GAAnn'nnTTC

Cuts at:      0     872    3567 
   Size:        872    2695
 
ZraI GAC'GTC

Cuts at:      0    2493    3567 
   Size:       2493    1074
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AfeI     AhdI     AleI     AlfI
    AlwNI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
    PflMI     PfoI     PmlI    PpuMI    PspXI     PsrI     PstI    PvuII
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfcI     SfoI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI    AflII   AflIII     AgeI     AloI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI    BbvCI     BcgI     BclI    BfrBI
     BmtI    BsiWI     BsmI    BsmBI   BssHII    BstXI  BstZ17I     ClaI
    CspCI     DraI     DrdI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI
   PspOMI     PvuI     SalI     SbfI     ScaI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI
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