Restriction map for pF1KA0197
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       AclI        AgeI        AhdI        AocI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbvCI       BcgI        BciVI       BlnI        
BsaI        BsiEI       BsiWI       BsmBI       BspDI       
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraIII      EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       FseI        FspI        HgaI        KspI        
MluI        MluNI       MscI        NaeI        NgoMIV      
NotI        NruI        PacI        PaeR7I      PflFI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SfiI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    StuI        
SwaI        Tth111I     XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I       1583  GGTACC               G^GTACC
AccI         2760  GTCTAC               GT^[AC][GT]AC
AccIII       1797  TCCGGA               T^CCGGA
AcyI          118  GGCGCC               G[GA]^CG[CT]C
AflII         616  CTTAAG               C^TTAAG
AflIII       2899  ACATGT               A^C[GA][CT]GT
Alw44I        376  GTGCAC               G^TGCAC
ApaI         1115  GGGCCC               GGGCC^C
ApaLI         376  GTGCAC               G^TGCAC
Asp718I      1583  GGTACC               G^GTACC
AvaI         1694  CTCGGG               C^[CT]CG[GA]G
BbeI          118  GGCGCC               GGCGC^C
BbrPI        3098  CACGTG               CAC^GTG
BfrI          616  CTTAAG               C^TTAAG
BglI         3858  GCCTAATTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        1227  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         118  GGCGCC               G[GA]^CG[CT]C
BsaWI        1797  TCCGGA               [AT]^CCGG[AT]
BseAI        1797  TCCGGA               T^CCGGA
BsoBI        1694  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     2559  TGTACA               T^GTACA
BspEI        1797  TCCGGA               T^CCGGA
BspHI        3378  TCATGA               T^CATGA
BspLU11I     2899  ACATGT               A^CATGT
BspMI        1180  GCAGGT               ACCTGC(4/8)
BsrGI        2559  TGTACA               T^GTACA
BstEII        724  GGTGACC              G^GT[ACGT]ACC
BstPI         724  GGTGACC              G^GT[ACGT]ACC
DrdI         3293  GACTTATTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       724  GGTGACC              G^GT[ACGT]ACC
HaeII         118  GGCGCC               [GA]GCGC^[CT]
Hin1I         118  GGCGCC               G[GA]^CG[CT]C
HpaI          360  GTTAAC               GTT^AAC
KasI          118  GGCGCC               G^GCGCC
KpnI         1583  GGTACC               GGTAC^C
MamI         1227  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1797  TCCGGA               T^CCGGA
NarI          118  GGCGCC               GG^CGCC
NcoI          947  CCATGG               C^CATGG
NheI         3696  GCTAGC               G^CTAGC
PflMI        3328  CCAGTGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        3098  CACGTG               CAC^GTG
PmlI         3098  CACGTG               CAC^GTG
RcaI         3378  TCATGA               T^CATGA
SexAI         639  ACCTGGT              A^CC[AT]GGT
SfoI          118  GGCGCC               GGC^GCC
SphI          975  GCATGC               GCATG^C
SspBI        2559  TGTACA               T^GTACA
Van91I       3328  CCAGTGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2529  TCTAGA               T^CTAGA
XcmI         3304  CCAGAATATGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccII          52  CGCG                 CG^CG
              141  CGCG                
AspHI         376  GTGCAC               G[AT]GC[AT]^C
             1017  GAGCAC              
AvaII        3114  GGACC                G^G[AT]CC
             3938  GGACC               
BanII          11  GAGCCC               G[GA]GC[CT]^C
             1115  GGGCCC              
BbsI          210  GAAGAC               GAAGAC(2/6)
             1499  GTCTTC              
BclI         1242  TGATCA               T^GATCA
             1832  TGATCA              
BcnI          404  CCCGG                CC^[GC]GG
             3240  CCGGG               
BglII        2091  AGATCT               A^GATCT
             3438  AGATCT              
BpuAI         210  GAAGAC               GAAGAC(2/6)
             1499  GTCTTC              
BsaAI         157  TACGTG               [CT]AC^GT[GA]
             3098  CACGTG              
BsiHKAI       376  GTGCAC               G[AT]GC[AT]^C
             1017  GAGCAC              
BsmI         3209  GAATGC               GAATGC(1/-1)
             3388  GAATGC              
BstAPI        684  GCAACGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1206  GCAGCCTCTGC         
BstUI          52  CGCG                 CG^CG
              141  CGCG                
DraII         572  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1115  GGGCCCT             
EcoO109I      572  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1115  GGGCCCT             
EcoRV        1108  GATATC               GAT^ATC
             1687  GATATC              
EcoT14I       947  CCATGG               C^C[AT][AT]GG
             3458  CCTTGG              
EcoT22I       973  ATGCAT               ATGCA^T
             3642  ATGCAT              
FbaI         1242  TGATCA               T^GATCA
             1832  TGATCA              
HincII        360  GTTAAC               GT[CT]^[GA]AC
              430  GTTGAC              
HindII        360  GTTAAC               GT[CT]^[GA]AC
              430  GTTGAC              
HindIII      1304  AAGCTT               A^AGCTT
             3601  AAGCTT              
MaeII         158  ACGT                 A^CGT
             3099  ACGT                
MvnI           52  CGCG                 CG^CG
              141  CGCG                
NciI          404  CCCGG                CC^[GC]GG
             3240  CCGGG               
NdeI         1704  CATATG               CA^TATG
             2945  CATATG              
NsiI          973  ATGCAT               ATGCA^T
             3642  ATGCAT              
PvuII        2110  CAGCTG               CAG^CTG
             3395  CAGCTG              
ScaI         2068  AGTACT               AGT^ACT
             2295  AGTACT              
SmlI          616  CTTAAG               C^T[CT][GA]AG
             3247  CTTGAG              
SspI          450  AATATT               AAT^ATT
             1987  AATATT              
StyI          947  CCATGG               C^C[AT][AT]GG
             3458  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        BanI        BbvI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstBI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EarI        EcoRI       EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MfeI        MflI        MnlI        MseI        MslI        
MspA1I      MspI        MunI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        NspV        
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfuI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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