Restriction map for pF1KA0066
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BalI        BbeI        BcgI        BciVI       
BfrI        BlnI        BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoT22I     
FauI        FseI        HaeII       Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII        2837  CGCG                 CG^CG
AgeI         2647  ACCGGT               A^CCGGT
AocI         1822  CCTAAGG              CC^T[ACGT]AGG
AvaI         2111  CCCGGG               C^[CT]CG[GA]G
AviII        2822  TGCGCA               TGC^GCA
BamHI         906  GGATCC               G^GATCC
BbrPI        1586  CACGTG               CAC^GTG
BclI         1397  TGATCA               T^GATCA
BglI         2701  GCCCAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2227  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     2227  GCTAAGC              GC^T[ACGT]AGC
BsaBI         901  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1658  GAGACC               GGTCTC(1/5)
BsaWI        2647  ACCGGT               [AT]^CCGG[AT]
BsoBI        2111  CCCGGG               C^[CT]CG[GA]G
BstUI        2837  CGCG                 CG^CG
Bsu36I       1822  CCTAAGG              CC^T[ACGT]AGG
CelII        2227  GCTAAGC              GC^T[ACGT]AGC
DraI          994  TTTAAA               TTT^AAA
DraIII        401  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1410  CCATGG               C^C[GA][CT]GG
EaeI         2805  TGGCCG               [CT]^GGCC[GA]
Eco81I       1822  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1437  CCTCTGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2812  GAATTC               G^AATTC
EcoRV         628  GATATC               GAT^ATC
FbaI         1397  TGATCA               T^GATCA
FspI         2822  TGCGCA               TGC^GCA
HgaI          409  GACGC                GACGC(5/10)
HincII       1017  GTCAAC               GT[CT]^[GA]AC
HindII       1017  GTCAAC               GT[CT]^[GA]AC
MamI          901  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         2837  CGCG                 CG^CG
NaeI         1107  GCCGGC               GCC^GGC
NcoI         1410  CCATGG               C^CATGG
NgoMIV       1107  GCCGGC               G^CCGGC
PflMI        1267  CCATTAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2647  ACCGGT               A^CCGGT
PmaCI        1586  CACGTG               CAC^GTG
PmlI         1586  CACGTG               CAC^GTG
PvuII        2360  CAGCTG               CAG^CTG
SacI         2534  GAGCTC               GAGCT^C
SapI         1954  GAAGAGC              GCTCTTC(1/4)
SgrAI        2646  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         2111  CCCGGG               CCC^GGG
SmlI         2368  CTCAAG               C^T[CT][GA]AG
SphI         2018  GCATGC               GCATG^C
Van91I       1267  CCATTAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2194  CCAAGCAATATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        2111  CCCGGG               C^CCGGG
XmaI         2111  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

BbsI         1318  GAAGAC               GAAGAC(2/6)
             2473  GAAGAC              
BbvCI         733  CCTCAGC              CCTCAGC(-5/-2)
             2866  CCTCAGC             
BpuAI        1318  GAAGAC               GAAGAC(2/6)
             2473  GAAGAC              
BsaAI         371  TACGTG               [CT]AC^GT[GA]
             1586  CACGTG              
BsmI         1759  GAATGC               GAATGC(1/-1)
             2697  GAATGC              
Bsp1407I      237  TGTACA               T^GTACA
             2876  TGTACA              
BspMI        2316  ACCTGC               ACCTGC(4/8)
             2911  GCAGGT              
BsrFI        1107  GCCGGC               [GA]^CCGG[CT]
             2647  ACCGGT              
BsrGI         237  TGTACA               T^GTACA
             2876  TGTACA              
BstAPI        303  GCAAGATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2320  GCAGACCTTGC         
BstBI         568  TTCGAA               TT^CGAA
              941  TTCGAA              
CfoI         2823  GCGC                 GCG^C
             2838  GCGC                
Cfr10I       1107  GCCGGC               [GA]^CCGG[CT]
             2647  ACCGGT              
DraII         546  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1818  AGGACCT             
EcoO109I      546  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1818  AGGACCT             
EcoT14I       464  CCTTGG               C^C[AT][AT]GG
             1410  CCATGG              
HhaI         2823  GCGC                 GCG^C
             2838  GCGC                
HinP1I       2823  GCGC                 G^CGC
             2838  GCGC                
MaeII         372  ACGT                 A^CGT
             1587  ACGT                
MslI         1180  CACAGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2826  CACCACTGTG          
MspA1I       2360  CAGCTG               C[AC]G^C[GT]G
             2869  CAGCGG              
NspI         2018  GCATGC               [GA]CATG^[CT]
             2664  ACATGC              
NspV          568  TTCGAA               TT^CGAA
              941  TTCGAA              
PpuMI         546  AGGTCCT              [GA]G^G[AT]CC[CT]
             1818  AGGACCT             
PstI         2318  CTGCAG               CTGCA^G
             2711  CTGCAG              
SfuI          568  TTCGAA               TT^CGAA
              941  TTCGAA              
SspBI         237  TGTACA               T^GTACA
             2876  TGTACA              
StyI          464  CCTTGG               C^C[AT][AT]GG
             1410  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BglII       
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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