Restriction map for pF1KA0066
 (Linear) (Six-base) MAPSORT of: KIAA0066.seq  Check: 7744  from: 1  to: 2943



KIAA0066.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2902    2943 
   Size:       2902      41
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1598    1796    1985    2390    2654    2943 
   Size:       1598     198     189     405     264     289

  Fragments arranged by size: 

                1598     405     289     264     198     189
 
AgeI A'CCGG_T

Cuts at:      0    2647    2943 
   Size:       2647     296
 
AleI CACnn'nnGTG

Cuts at:      0    1184    2830    2943 
   Size:       1184    1646     113

  Fragments arranged by size: 

                1646    1184     113
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2173    2848    2943 
   Size:       2173     675      95

  Fragments arranged by size: 

                2173     675      95
 
ApoI r'AATT_y

Cuts at:      0     191     503     988     998    1447    1750    1836    2812 
   Size:        191     312     485      10     449     303      86     976

Cuts at:   2812    2943 
   Size:        131

  Fragments arranged by size: 

                 976     485     449     312     303     191     131      86
                  10
 
AvaI C'yCGr_G

Cuts at:      0    2111    2943 
   Size:       2111     832
 
BamHI G'GATC_C

Cuts at:      0     906    2943 
   Size:        906    2037
 
BanI G'GyrC_C

Cuts at:      0     486     808    1668    2333    2914    2943 
   Size:        486     322     860     665     581      29

  Fragments arranged by size: 

                 860     665     581     486     322      29
 
BanII G_rGCy'C

Cuts at:      0      20    2004    2538    2755    2943 
   Size:         20    1984     534     217     188

  Fragments arranged by size: 

                1984     534     217     188      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1325    2480    2943 
   Size:       1325    1155     463

  Fragments arranged by size: 

                1325    1155     463
 
BbvCI CC'TCA_GC

Cuts at:      0     734    2867    2943 
   Size:        734    2133      76

  Fragments arranged by size: 

                2133     734      76
 
BclI T'GATC_A

Cuts at:      0    1397    2943 
   Size:       1397    1546
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2707    2943 
   Size:       2707     236
 
BglII A'GATC_T

Cuts at:      0    1518    1944    2595    2943 
   Size:       1518     426     651     348

  Fragments arranged by size: 

                1518     651     426     348
 
BlpI GC'TnA_GC

Cuts at:      0    2228    2943 
   Size:       2228     715
 
Bme1580I G_kGCm'C

Cuts at:      0    2181    2943 
   Size:       2181     762
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1918    2943 
   Size:       1918    1025
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     656     728    1752    2644    2943 
   Size:        656      72    1024     892     299

  Fragments arranged by size: 

                1024     892     656     299      72
 
Bpu10I CC'TnA_GC

Cuts at:      0     734    2867    2943 
   Size:        734    2133      76

  Fragments arranged by size: 

                2133     734      76
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2353    2943 
   Size:       2353     590
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1652    2943 
   Size:       1652    1291
 
BsaAI yAC'GTr

Cuts at:      0     373    1588    2943 
   Size:        373    1215    1355

  Fragments arranged by size: 

                1355    1215     373
 
BsaBI GATnn'nnATC

Cuts at:      0     905    2943 
   Size:        905    2038
 
BsaWI w'CCGG_w

Cuts at:      0    2647    2943 
   Size:       2647     296
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     758     788    2943 
   Size:        758      30    2155

  Fragments arranged by size: 

                2155     758      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     782    1207    2143    2596    2746    2943 
   Size:        782     425     936     453     150     197

  Fragments arranged by size: 

                 936     782     453     425     197     150
 
BseYI C'CCAG_C

Cuts at:      0     312    2943 
   Size:        312    2631
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383    1334    2278    2672    2943 
   Size:        383     951     944     394     271

  Fragments arranged by size: 

                 951     944     394     383     271
 
BsiHKAI G_wGCw'C

Cuts at:      0     345    2016    2538    2943 
   Size:        345    1671     522     405

  Fragments arranged by size: 

                1671     522     405     345
 
BsmI GAATG_Cn'

Cuts at:      0    1765    2703    2943 
   Size:       1765     938     240

  Fragments arranged by size: 

                1765     938     240
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     345    2004    2016    2181    2538    2755    2943 
   Size:         20     325    1659      12     165     357     217     188

  Fragments arranged by size: 

                1659     357     325     217     188     165      20      12
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2325    2902    2943 
   Size:       2325     577      41

  Fragments arranged by size: 

                2325     577      41
 
BsrDI GCAATG_nn'

Cuts at:      0     345     664    2157    2525    2943 
   Size:        345     319    1493     368     418

  Fragments arranged by size: 

                1493     418     368     345     319
 
BsrFI r'CCGG_y

Cuts at:      0    1107    2647    2943 
   Size:       1107    1540     296

  Fragments arranged by size: 

                1540    1107     296
 
BsrGI T'GTAC_A

Cuts at:      0     237    2876    2943 
   Size:        237    2639      67

  Fragments arranged by size: 

                2639     237      67
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     309    2326    2943 
   Size:        309    2017     617

  Fragments arranged by size: 

                2017     617     309
 
BstBI TT'CG_AA

Cuts at:      0     569     942    2943 
   Size:        569     373    2001

  Fragments arranged by size: 

                2001     569     373
 
BstYI r'GATC_y

Cuts at:      0     819     906    1518    1944    2595    2943 
   Size:        819      87     612     426     651     348

  Fragments arranged by size: 

                 819     651     612     426     348      87
 
Bsu36I CC'TnA_GG

Cuts at:      0    1823    2943 
   Size:       1823    1120
 
BtgI C'CryG_G

Cuts at:      0    1410    2943 
   Size:       1410    1533
 
BtsI GCAGTG_nn'

Cuts at:      0      53    1154    1879    2943 
   Size:         53    1101     725    1064

  Fragments arranged by size: 

                1101    1064     725      53
 
DraI TTT'AAA

Cuts at:      0     996    2943 
   Size:        996    1947
 
DraIII CAC_nnn'GTG

Cuts at:      0     406    2943 
   Size:        406    2537
 
EaeI y'GGCC_r

Cuts at:      0    2805    2943 
   Size:       2805     138
 
EarI CTCTTCn'nnn_

Cuts at:      0     170    1949    2120    2652    2737    2943 
   Size:        170    1779     171     532      85     206

  Fragments arranged by size: 

                1779     532     206     171     170      85
 
EcoICRI GAG'CTC

Cuts at:      0    2536    2943 
   Size:       2536     407
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     656     728    1598    1752    1796    1985    2390    2644 
   Size:        656      72     870     154      44     189     405     254

Cuts at:   2644    2654    2943 
   Size:         10     289

  Fragments arranged by size: 

                 870     656     405     289     254     189     154      72
                  44      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1441    2943 
   Size:       1441    1502
 
EcoO109I rG'GnC_Cy

Cuts at:      0     547    1819    2943 
   Size:        547    1272    1124

  Fragments arranged by size: 

                1272    1124     547
 
EcoRI G'AATT_C

Cuts at:      0    2812    2943 
   Size:       2812     131
 
EcoRV GAT'ATC

Cuts at:      0     630    2943 
   Size:        630    2313
 
FspI TGC'GCA

Cuts at:      0    2824    2943 
   Size:       2824     119
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       7      39     636     668    1096    1128    1929    1961 
   Size:          7      32     597      32     428      32     801      32

Cuts at:   1961    2016    2035    2048    2067    2943 
   Size:         55      19      13      19     876

  Fragments arranged by size: 

                 876     801     597     428      55      32      32      32
                  32      19      19      13       7
 
HincII GTy'rAC

Cuts at:      0    1019    2943 
   Size:       1019    1924
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     756    2943 
   Size:        756    2187
 
MslI CAynn'nnrTG

Cuts at:      0    1184    2830    2943 
   Size:       1184    1646     113

  Fragments arranged by size: 

                1646    1184     113
 
MspA1I CmG'CkG

Cuts at:      0    2362    2871    2943 
   Size:       2362     509      72

  Fragments arranged by size: 

                2362     509      72
 
NaeI GCC'GGC

Cuts at:      0    1109    2943 
   Size:       1109    1834
 
NcoI C'CATG_G

Cuts at:      0    1410    2943 
   Size:       1410    1533
 
NgoMIV G'CCGG_C

Cuts at:      0    1107    2943 
   Size:       1107    1836
 
NspI r_CATG'y

Cuts at:      0    2022    2668    2943 
   Size:       2022     646     275

  Fragments arranged by size: 

                2022     646     275
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1273    2943 
   Size:       1273    1670
 
PfoI T'CCnGG_A

Cuts at:      0    1494    1729    2943 
   Size:       1494     235    1214

  Fragments arranged by size: 

                1494    1214     235
 
PmlI CAC'GTG

Cuts at:      0    1588    2943 
   Size:       1588    1355
 
PpuMI rG'GwC_Cy

Cuts at:      0     547    1819    2943 
   Size:        547    1272    1124

  Fragments arranged by size: 

                1272    1124     547
 
PstI C_TGCA'G

Cuts at:      0    2322    2715    2943 
   Size:       2322     393     228

  Fragments arranged by size: 

                2322     393     228
 
PvuII CAG'CTG

Cuts at:      0    2362    2943 
   Size:       2362     581
 
SacI G_AGCT'C

Cuts at:      0    2538    2943 
   Size:       2538     405
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1949    2943 
   Size:       1949     994
 
SfcI C'TryA_G

Cuts at:      0    1549    2318    2711    2943 
   Size:       1549     769     393     232

  Fragments arranged by size: 

                1549     769     393     232
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2647    2943 
   Size:       2647     296
 
SmaI CCC'GGG

Cuts at:      0    2113    2943 
   Size:       2113     830
 
SmlI C'TyrA_G

Cuts at:      0    2368    2943 
   Size:       2368     575
 
SphI G_CATG'C

Cuts at:      0    2022    2943 
   Size:       2022     921
 
StyI C'CwwG_G

Cuts at:      0     464    1410    2943 
   Size:        464     946    1533

  Fragments arranged by size: 

                1533     946     464
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     880    2528    2943 
   Size:        880    1648     415

  Fragments arranged by size: 

                1648     880     415
 
TatI w'GTAC_w

Cuts at:      0     237     602     702    2364    2876    2943 
   Size:        237     365     100    1662     512      67

  Fragments arranged by size: 

                1662     512     365     237     100      67
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2865    2897    2943 
   Size:       2865      32      46

  Fragments arranged by size: 

                2865      46      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2201    2943 
   Size:       2201     742
 
XmaI C'CCGG_G

Cuts at:      0    2111    2943 
   Size:       2111     832
 
XmnI GAAnn'nnTTC

Cuts at:      0     575    1281    1451    2495    2943 
   Size:        575     706     170    1044     448

  Fragments arranged by size: 

                1044     706     575     448     170
 

 Enzymes that do cut:

     AarI     AcuI     AgeI     AleI     AlfI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI     BclI     BglI    BglII     BlpI
 Bme1580I     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI    BsrFI    BsrGI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI     BtsI     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FspI    Hin4I   HincII     MmeI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI    PflMI     PfoI
     PmlI    PpuMI     PstI    PvuII     SacI     SapI     SfcI    SgrAI
     SmaI     SmlI     SphI     StyI    TaqII     TatI     TstI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI    BfrBI    BmgBI     BmrI     BmtI
    BsaHI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI   BssHII
    BssSI   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DrdI
     EagI     EciI     FalI     FseI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NarI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI     ZraI
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