Restriction map for pF1KA0010
 (Linear) (Six-base) MAPSORT of: KIAA0010.seq  Check: 4139  from: 1  to: 3249



KIAA0010.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3157    3249 
   Size:       3157      92
 
Acc65I G'GTAC_C

Cuts at:      0    1317    1425    3249 
   Size:       1317     108    1824

  Fragments arranged by size: 

                1824    1317     108
 
AclI AA'CG_TT

Cuts at:      0     336    3249 
   Size:        336    2913
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1207    1420    2362    2638    3055    3184    3249 
   Size:       1207     213     942     276     417     129      65

  Fragments arranged by size: 

                1207     942     417     276     213     129      65
 
AflIII A'CryG_T

Cuts at:      0    1944    3249 
   Size:       1944    1305
 
AleI CACnn'nnGTG

Cuts at:      0    2665    3249 
   Size:       2665     584
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     478    3175    3249 
   Size:        478    2697      74

  Fragments arranged by size: 

                2697     478      74
 
AlwNI CAG_nnn'CTG

Cuts at:      0     303    3149    3249 
   Size:        303    2846     100

  Fragments arranged by size: 

                2846     303     100
 
ApaI G_GGCC'C

Cuts at:      0     996    3249 
   Size:        996    2253
 
ApaLI G'TGCA_C

Cuts at:      0    2260    2897    3249 
   Size:       2260     637     352

  Fragments arranged by size: 

                2260     637     352
 
ApoI r'AATT_y

Cuts at:      0     590     704    1552    1857    2604    2707    2876    3249 
   Size:        590     114     848     305     747     103     169     373

  Fragments arranged by size: 

                 848     747     590     373     305     169     114     103
            
 
AseI AT'TA_AT

Cuts at:      0    2168    2887    3249 
   Size:       2168     719     362

  Fragments arranged by size: 

                2168     719     362
 
AvaI C'yCGr_G

Cuts at:      0     686    2845    3172    3249 
   Size:        686    2159     327      77

  Fragments arranged by size: 

                2159     686     327      77
 
BamHI G'GATC_C

Cuts at:      0    2252    3249 
   Size:       2252     997
 
BanI G'GyrC_C

Cuts at:      0    1317    1425    3249 
   Size:       1317     108    1824

  Fragments arranged by size: 

                1824    1317     108
 
BanII G_rGCy'C

Cuts at:      0     887     996    3249 
   Size:        887     109    2253

  Fragments arranged by size: 

                2253     887     109
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     332    1254    2636    2976    3249 
   Size:        332     922    1382     340     273

  Fragments arranged by size: 

                1382     922     340     332     273
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1637    1671    3249 
   Size:       1637      34    1578

  Fragments arranged by size: 

                1637    1578      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3098    3249 
   Size:       3098     151
 
BclI T'GATC_A

Cuts at:      0    1409    2865    3249 
   Size:       1409    1456     384

  Fragments arranged by size: 

                1456    1409     384
 
BfrBI ATG'CAT

Cuts at:      0    1654    1769    3249 
   Size:       1654     115    1480

  Fragments arranged by size: 

                1654    1480     115
 
BglII A'GATC_T

Cuts at:      0    2099    3249 
   Size:       2099    1150
 
BlpI GC'TnA_GC

Cuts at:      0    3244    3249 
   Size:       3244       5
 
Bme1580I G_kGCm'C

Cuts at:      0     996    2264    2901    3249 
   Size:        996    1268     637     348

  Fragments arranged by size: 

                1268     996     637     348
 
BmgBI CAC'GTC

Cuts at:      0    2001    2559    3249 
   Size:       2001     558     690

  Fragments arranged by size: 

                2001     690     558
 
BmtI G_CTAG'C

Cuts at:      0     566    3249 
   Size:        566    2683
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2207    3009    3249 
   Size:       2207     802     240

  Fragments arranged by size: 

                2207     802     240
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1119    3145    3249 
   Size:       1119    2026     104

  Fragments arranged by size: 

                2026    1119     104
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     632    3249 
   Size:        632    2617
 
BsaWI w'CCGG_w

Cuts at:      0     756    2744    3249 
   Size:        756    1988     505

  Fragments arranged by size: 

                1988     756     505
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2563    2593    3100    3130    3249 
   Size:       2563      30     507      30     119

  Fragments arranged by size: 

                2563     507     119      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     811    1120    1123    1270    1866    2656    3249 
   Size:        811     309       3     147     596     790     593

  Fragments arranged by size: 

                 811     790     596     593     309     147       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1321    3249 
   Size:       1321    1928
 
BsiHKAI G_wGCw'C

Cuts at:      0     945    2264    2901    3249 
   Size:        945    1319     637     348

  Fragments arranged by size: 

                1319     945     637     348
 
BsmI GAATG_Cn'

Cuts at:      0     515     900    1186    2474    3249 
   Size:        515     385     286    1288     775

  Fragments arranged by size: 

                1288     775     515     385     286
 
Bsp1286I G_dGCh'C

Cuts at:      0     887     945     996    2264    2901    3249 
   Size:        887      58      51    1268     637     348

  Fragments arranged by size: 

                1268     887     637     348      58      51
 
BspEI T'CCGG_A

Cuts at:      0     756    3249 
   Size:        756    2493
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     446    2682    3157    3167    3249 
   Size:        446    2236     475      10      82

  Fragments arranged by size: 

                2236     475     446      82      10
 
BsrBI CCG'CTC

Cuts at:      0    3129    3249 
   Size:       3129     120
 
BsrDI GCAATG_nn'

Cuts at:      0     260     730    3249 
   Size:        260     470    2519

  Fragments arranged by size: 

                2519     470     260
 
BssSI C'ACGA_G

Cuts at:      0    1848    3249 
   Size:       1848    1401
 
BstBI TT'CG_AA

Cuts at:      0      11    3249 
   Size:         11    3238
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1877    3249 
   Size:       1877    1372
 
BstYI r'GATC_y

Cuts at:      0     400    2099    2252    2792    3249 
   Size:        400    1699     153     540     457

  Fragments arranged by size: 

                1699     540     457     400     153
 
Bsu36I CC'TnA_GG

Cuts at:      0    2591    3249 
   Size:       2591     658
 
BtgI C'CryG_G

Cuts at:      0    1271    2277    3249 
   Size:       1271    1006     972

  Fragments arranged by size: 

                1271    1006     972
 
BtsI GCAGTG_nn'

Cuts at:      0    2802    3249 
   Size:       2802     447
 
DraI TTT'AAA

Cuts at:      0    2330    3249 
   Size:       2330     919
 
DraIII CAC_nnn'GTG

Cuts at:      0    2766    3249 
   Size:       2766     483
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2728    3249 
   Size:       2728     521
 
EarI CTCTTCn'nnn_

Cuts at:      0    1613    2614    3249 
   Size:       1613    1001     635

  Fragments arranged by size: 

                1613    1001     635
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1977    2785    2806    3249 
   Size:       1977     808      21     443

  Fragments arranged by size: 

                1977     808     443      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1207    1420    2207    2362    2638    3009    3055    3184 
   Size:       1207     213     787     155     276     371      46     129

Cuts at:   3184    3249 
   Size:         65

  Fragments arranged by size: 

                1207     787     371     276     213     155     129      65
                  46
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1000    2683    3249 
   Size:       1000    1683     566

  Fragments arranged by size: 

                1683    1000     566
 
EcoO109I rG'GnC_Cy

Cuts at:      0     992     993    2823    2922    3249 
   Size:        992       1    1830      99     327

  Fragments arranged by size: 

                1830     992     327      99       1
 
EcoRI G'AATT_C

Cuts at:      0    1552    3249 
   Size:       1552    1697
 
EcoRV GAT'ATC

Cuts at:      0    1445    3249 
   Size:       1445    1804
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1348    3249 
   Size:       1348    1901
 
FspI TGC'GCA

Cuts at:      0     863    3249 
   Size:        863    2386
 
HaeII r_GCGC'y

Cuts at:      0     254     853    2741    3249 
   Size:        254     599    1888     508

  Fragments arranged by size: 

                1888     599     508     254
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1117    1149    2084    2116    2914    2946    3050    3082 
   Size:       1117      32     935      32     798      32     104      32

Cuts at:   3082    3249 
   Size:        167

  Fragments arranged by size: 

                1117     935     798     167     104      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1400    3249 
   Size:       1400    1849
 
HindIII A'AGCT_T

Cuts at:      0     652    3249 
   Size:        652    2597
 
KpnI G_GTAC'C

Cuts at:      0    1321    1429    3249 
   Size:       1321     108    1820

  Fragments arranged by size: 

                1820    1321     108
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     374    3143    3249 
   Size:        374    2769     106

  Fragments arranged by size: 

                2769     374     106
 
MslI CAynn'nnrTG

Cuts at:      0    1270    1294    1774    2500    2665    3151    3249 
   Size:       1270      24     480     726     165     486      98

  Fragments arranged by size: 

                1270     726     486     480     165      98      24
 
MspA1I CmG'CkG

Cuts at:      0    1983    2022    3232    3249 
   Size:       1983      39    1210      17

  Fragments arranged by size: 

                1983    1210      39      17
 
NcoI C'CATG_G

Cuts at:      0    1271    2277    3249 
   Size:       1271    1006     972

  Fragments arranged by size: 

                1271    1006     972
 
NheI G'CTAG_C

Cuts at:      0     562    3249 
   Size:        562    2687
 
NsiI A_TGCA'T

Cuts at:      0    1656    1771    3249 
   Size:       1656     115    1478

  Fragments arranged by size: 

                1656    1478     115
 
NspI r_CATG'y

Cuts at:      0     419    1658    1948    2499    3249 
   Size:        419    1239     290     551     750

  Fragments arranged by size: 

                1239     750     551     419     290
 
PacI TTA_AT'TAA

Cuts at:      0     365    3249 
   Size:        365    2884
 
PciI A'CATG_T

Cuts at:      0    1944    3249 
   Size:       1944    1305
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2283    2766    3249 
   Size:       2283     483     483

  Fragments arranged by size: 

                2283     483     483
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     906     938    3249 
   Size:        906      32    2311

  Fragments arranged by size: 

                2311     906      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2922    3249 
   Size:       2922     327
 
PspOMI G'GGCC_C

Cuts at:      0     992    3249 
   Size:        992    2257
 
PspXI vC'TCGA_Gb

Cuts at:      0    2845    3249 
   Size:       2845     404
 
PstI C_TGCA'G

Cuts at:      0    1037    1988    2964    3249 
   Size:       1037     951     976     285

  Fragments arranged by size: 

                1037     976     951     285
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1613    2614    3249 
   Size:       1613    1001     635

  Fragments arranged by size: 

                1613    1001     635
 
SexAI A'CCwGG_T

Cuts at:      0    1229    3249 
   Size:       1229    2020
 
SfcI C'TryA_G

Cuts at:      0     174     695    1033    1338    1984    2775    2960    3249 
   Size:        174     521     338     305     646     791     185     289

  Fragments arranged by size: 

                 791     646     521     338     305     289     185     174
            
 
SmlI C'TyrA_G

Cuts at:      0     686    1134    2845    3124    3249 
   Size:        686     448    1711     279     125

  Fragments arranged by size: 

                1711     686     448     279     125
 
SphI G_CATG'C

Cuts at:      0    1658    3249 
   Size:       1658    1591
 
SspI AAT'ATT

Cuts at:      0     196     673    3249 
   Size:        196     477    2576

  Fragments arranged by size: 

                2576     477     196
 
StyI C'CwwG_G

Cuts at:      0      35      45    1271    2277    3249 
   Size:         35      10    1226    1006     972

  Fragments arranged by size: 

                1226    1006     972      35      10
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2563    2595    3249 
   Size:       2563      32     654

  Fragments arranged by size: 

                2563     654      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1998    2556    3249 
   Size:       1998     558     693

  Fragments arranged by size: 

                1998     693     558
 
XhoI C'TCGA_G

Cuts at:      0     686    2845    3249 
   Size:        686    2159     404

  Fragments arranged by size: 

                2159     686     404
 
XmnI GAAnn'nnTTC

Cuts at:      0    2393    3249 
   Size:       2393     856
 

 Enzymes that do cut:

     AarI   Acc65I     AclI     AcuI   AflIII     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI     AvaI    BamHI     BanI    BanII
     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BpmI    BpuEI     BsaI    BsaWI    BsaXI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BssSI    BstBI    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI
   DraIII     DrdI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KpnI
     MmeI     MslI   MspA1I     NcoI     NheI     NsiI     NspI     PacI
     PciI    PflMI     PpiI    PpuMI   PspOMI    PspXI     PstI     SapI
    SexAI     SfcI     SmlI     SphI     SspI     StyI     TstI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AfeI    AflII     AgeI     AhdI     AloI     AscI
    AsiSI    AvrII     BaeI     BbeI    BbvCI     BglI     BmrI     BplI
   Bpu10I    BsaAI    BsaBI    BsaHI    BseYI    BsiEI    BsiWI    BsmBI
    BspHI    BsrFI    BsrGI   BssHII   BstAPI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     EaeI     EagI  EcoICRI     FseI    FspAI     HpaI
     KasI     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV
     NotI     NruI     PasI     PfoI     PmeI     PmlI    PshAI     PsiI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XmaI     ZraI
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