Restriction map for
pF1KSDB1426
(Linear) (Six-base) MAPSORT of: KIBB1426.seq Check: 2998 from: 1 to: 2043
KIBB1426.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AatII G_ACGT'C
Cuts at: 0 1664 2043
Size: 1664 379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 936 1730 1788 2043
Size: 787 149 794 58 255
Fragments arranged by size:
794 787 255 149 58
AfeI AGC'GCT
Cuts at: 0 571 2043
Size: 571 1472
AflIII A'CryG_T
Cuts at: 0 380 2043
Size: 380 1663
AleI CACnn'nnGTG
Cuts at: 0 181 1741 2043
Size: 181 1560 302
Fragments arranged by size:
1560 302 181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 241 602 1357 2043
Size: 241 361 755 686
Fragments arranged by size:
755 686 361 241
AlwNI CAG_nnn'CTG
Cuts at: 0 339 537 1539 2043
Size: 339 198 1002 504
Fragments arranged by size:
1002 504 339 198
ApaI G_GGCC'C
Cuts at: 0 544 2043
Size: 544 1499
AvaI C'yCGr_G
Cuts at: 0 246 481 752 1271 1284 2043
Size: 246 235 271 519 13 759
Fragments arranged by size:
759 519 271 246 235 13
BamHI G'GATC_C
Cuts at: 0 1048 2043
Size: 1048 995
BanI G'GyrC_C
Cuts at: 0 143 559 586 631 1123 2001 2043
Size: 143 416 27 45 492 878 42
Fragments arranged by size:
878 492 416 143 45 42 27
BanII G_rGCy'C
Cuts at: 0 397 496 544 604 706 1249 1464 1955
Size: 397 99 48 60 102 543 215 491
Cuts at: 1955 2043
Size: 88
Fragments arranged by size:
543 491 397 215 102 99 88 60
48
BbeI G_GCGC'C
Cuts at: 0 147 2043
Size: 147 1896
BbsI GAAGACnn'nnnn_
Cuts at: 0 208 1028 2043
Size: 208 820 1015
Fragments arranged by size:
1015 820 208
BbvCI CC'TCA_GC
Cuts at: 0 433 2043
Size: 433 1610
BciVI GTATCCnnnnn_n'
Cuts at: 0 966 2043
Size: 966 1077
BclI T'GATC_A
Cuts at: 0 1361 2043
Size: 1361 682
BglI GCCn_nnn'nGGC
Cuts at: 0 513 701 2043
Size: 513 188 1342
Fragments arranged by size:
1342 513 188
BglII A'GATC_T
Cuts at: 0 32 2043
Size: 32 2011
BlpI GC'TnA_GC
Cuts at: 0 705 2043
Size: 705 1338
Bme1580I G_kGCm'C
Cuts at: 0 333 451 544 634 1128 2006 2043
Size: 333 118 93 90 494 878 37
Fragments arranged by size:
878 494 333 118 93 90 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 372 393 912 1810 1879 2043
Size: 372 21 519 898 69 164
Fragments arranged by size:
898 519 372 164 69 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1299 1480 2043
Size: 1299 181 563
Fragments arranged by size:
1299 563 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 373 1704 1924 2043
Size: 175 198 1331 220 119
Fragments arranged by size:
1331 220 198 175 119
Bpu10I CC'TnA_GC
Cuts at: 0 433 1180 1556 2043
Size: 433 747 376 487
Fragments arranged by size:
747 487 433 376
BsaI GGTCTCn'nnnn_
Cuts at: 0 1760 1884 2043
Size: 1760 124 159
Fragments arranged by size:
1760 159 124
BsaAI yAC'GTr
Cuts at: 0 1230 2043
Size: 1230 813
BsaHI Gr'CG_yC
Cuts at: 0 144 1661 2043
Size: 144 1517 382
Fragments arranged by size:
1517 382 144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 801 831 1462 1492 1783 1813 1864 1894
Size: 801 30 631 30 291 30 51 30
Cuts at: 1894 2043
Size: 149
Fragments arranged by size:
801 631 291 149 51 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 688 855 1438 1472 1513 2043
Size: 688 167 583 34 41 530
Fragments arranged by size:
688 583 530 167 41 34
BseYI C'CCAG_C
Cuts at: 0 348 582 1041 2043
Size: 348 234 459 1002
Fragments arranged by size:
1002 459 348 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 2043
Size: 205 1838
BsiEI CG_ry'CG
Cuts at: 0 877 2043
Size: 877 1166
BsiHKAI G_wGCw'C
Cuts at: 0 362 604 1439 1647 1955 2043
Size: 362 242 835 208 308 88
Fragments arranged by size:
835 362 308 242 208 88
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1482 2043
Size: 1482 561
Bsp1286I G_dGCh'C
Cuts at: 0 333 362 397 451 496 544 604 634
Size: 333 29 35 54 45 48 60 30
Cuts at: 634 706 1128 1249 1439 1464 1647 1955 2006
Size: 72 422 121 190 25 183 308 51
Cuts at: 2006 2043
Size: 37
Fragments arranged by size:
422 333 308 190 183 121 72 60
54 51 48 45 37 35 30 29
25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1103 1556 2043
Size: 1103 453 487
Fragments arranged by size:
1103 487 453
BsrBI CCG'CTC
Cuts at: 0 1908 2043
Size: 1908 135
BsrFI r'CCGG_y
Cuts at: 0 320 939 1031 1252 2043
Size: 320 619 92 221 791
Fragments arranged by size:
791 619 320 221 92
BsrGI T'GTAC_A
Cuts at: 0 1400 2043
Size: 1400 643
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1262 2043
Size: 1262 781
BstEII G'GTnAC_C
Cuts at: 0 1201 2043
Size: 1201 842
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 516 1743 2043
Size: 156 360 1227 300
Fragments arranged by size:
1227 360 300 156
BstYI r'GATC_y
Cuts at: 0 32 1048 2043
Size: 32 1016 995
Fragments arranged by size:
1016 995 32
Bsu36I CC'TnA_GG
Cuts at: 0 1318 2043
Size: 1318 725
BtgI C'CryG_G
Cuts at: 0 155 420 475 496 626 1109 2043
Size: 155 265 55 21 130 483 934
Fragments arranged by size:
934 483 265 155 130 55 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 238 2043
Size: 238 1805
BtsI GCAGTG_nn'
Cuts at: 0 136 800 2043
Size: 136 664 1243
Fragments arranged by size:
1243 664 136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 887 922 2043
Size: 887 35 1121
Fragments arranged by size:
1121 887 35
DraIII CAC_nnn'GTG
Cuts at: 0 1517 2043
Size: 1517 526
EaeI y'GGCC_r
Cuts at: 0 303 682 908 1010 1298 2043
Size: 303 379 226 102 288 745
Fragments arranged by size:
745 379 303 288 226 102
EarI CTCTTCn'nnn_
Cuts at: 0 1351 1825 2043
Size: 1351 474 218
Fragments arranged by size:
1351 474 218
EcoICRI GAG'CTC
Cuts at: 0 602 1953 2043
Size: 602 1351 90
Fragments arranged by size:
1351 602 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 373 787 936 1704 1730 1788 1924
Size: 175 198 414 149 768 26 58 136
Cuts at: 1924 2043
Size: 119
Fragments arranged by size:
768 414 198 175 149 136 119 58
26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1099 2043
Size: 1099 944
EcoO109I rG'GnC_Cy
Cuts at: 0 501 541 838 947 1104 1536 1923 2043
Size: 501 40 297 109 157 432 387 120
Fragments arranged by size:
501 432 387 297 157 120 109 40
EcoRV GAT'ATC
Cuts at: 0 1749 2043
Size: 1749 294
HaeII r_GCGC'y
Cuts at: 0 43 147 573 2043
Size: 43 104 426 1470
Fragments arranged by size:
1470 426 104 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1462 1494 1738 1770 2043
Size: 1462 32 244 32 273
Fragments arranged by size:
1462 273 244 32 32
KasI G'GCGC_C
Cuts at: 0 143 2043
Size: 143 1900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 897 2043
Size: 314 583 1146
Fragments arranged by size:
1146 583 314
MscI TGG'CCA
Cuts at: 0 910 2043
Size: 910 1133
MslI CAynn'nnrTG
Cuts at: 0 181 1741 1768 2043
Size: 181 1560 27 275
Fragments arranged by size:
1560 275 181 27
MspA1I CmG'CkG
Cuts at: 0 437 582 1045 2043
Size: 437 145 463 998
Fragments arranged by size:
998 463 437 145
NaeI GCC'GGC
Cuts at: 0 322 2043
Size: 322 1721
NarI GG'CG_CC
Cuts at: 0 144 2043
Size: 144 1899
NgoMIV G'CCGG_C
Cuts at: 0 320 2043
Size: 320 1723
NruI TCG'CGA
Cuts at: 0 171 2043
Size: 171 1872
NspI r_CATG'y
Cuts at: 0 384 792 1555 2043
Size: 384 408 763 488
Fragments arranged by size:
763 488 408 384
PasI CC'CwG_GG
Cuts at: 0 339 537 2043
Size: 339 198 1506
Fragments arranged by size:
1506 339 198
PciI A'CATG_T
Cuts at: 0 380 2043
Size: 380 1663
PflMI CCAn_nnn'nTGG
Cuts at: 0 942 2043
Size: 942 1101
PfoI T'CCnGG_A
Cuts at: 0 128 1838 1901 2043
Size: 128 1710 63 142
Fragments arranged by size:
1710 142 128 63
PpuMI rG'GwC_Cy
Cuts at: 0 501 947 1104 1923 2043
Size: 501 446 157 819 120
Fragments arranged by size:
819 501 446 157 120
PspOMI G'GGCC_C
Cuts at: 0 540 2043
Size: 540 1503
PstI C_TGCA'G
Cuts at: 0 581 599 1454 1535 1979 2043
Size: 581 18 855 81 444 64
Fragments arranged by size:
855 581 444 81 64 18
PvuII CAG'CTG
Cuts at: 0 437 582 2043
Size: 437 145 1461
Fragments arranged by size:
1461 437 145
SacI G_AGCT'C
Cuts at: 0 604 1955 2043
Size: 604 1351 88
Fragments arranged by size:
1351 604 88
SanDI GG'GwC_CC
Cuts at: 0 1923 2043
Size: 1923 120
SapI GCTCTTCn'nnn_
Cuts at: 0 1351 2043
Size: 1351 692
SbfI CC_TGCA'GG
Cuts at: 0 1454 2043
Size: 1454 589
SexAI A'CCwGG_T
Cuts at: 0 1412 2043
Size: 1412 631
SfcI C'TryA_G
Cuts at: 0 577 595 1450 1531 1975 2043
Size: 577 18 855 81 444 68
Fragments arranged by size:
855 577 444 81 68 18
SfoI GGC'GCC
Cuts at: 0 145 2043
Size: 145 1898
SmaI CCC'GGG
Cuts at: 0 754 1273 2043
Size: 754 519 770
Fragments arranged by size:
770 754 519
SphI G_CATG'C
Cuts at: 0 1555 2043
Size: 1555 488
StyI C'CwwG_G
Cuts at: 0 911 1118 2043
Size: 911 207 925
Fragments arranged by size:
925 911 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 574 1422 1869 2043
Size: 574 848 447 174
Fragments arranged by size:
848 574 447 174
TatI w'GTAC_w
Cuts at: 0 1400 2043
Size: 1400 643
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 35 67 801 833 1864 1896 2043
Size: 35 32 734 32 1031 32 147
Fragments arranged by size:
1031 734 147 35 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1474 2043
Size: 1474 569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 204 1125 2043
Size: 156 48 921 918
Fragments arranged by size:
921 918 156 48
XmaI C'CCGG_G
Cuts at: 0 752 1271 2043
Size: 752 519 772
Fragments arranged by size:
772 752 519
ZraI GAC'GTC
Cuts at: 0 1662 2043
Size: 1662 381
Enzymes that do cut:
AatII AcuI AfeI AflIII AleI AlfI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PasI PciI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI
SbfI SexAI SfcI SfoI SmaI SphI StyI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI
BfrBI BmgBI BmtI BpuEI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BsrDI BssHII BssSI BstBI BstZ17I ClaI
DraI DrdI EagI EciI EcoRI FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NotI NsiI PacI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI StuI SwaI XbaI XhoI XmnI