Restriction map for
pF1KSDB1384
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvrII
BamHI BanI BbeI BbrPI BcgI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT14I
FseI HaeII HgaI Hin1I KasI
KpnI KspI MamI MluI MroI
MspA1I NarI NcoI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1804 GTCGAC GT^[AC][GT]AC
AviII 471 TGCGCA TGC^GCA
BalI 2 TGGCCA TGG^CCA
BanII 1332 GAGCCC G[GA]GC[CT]^C
BciVI 863 GGATAC GTATCC(6/5)
BclI 1467 TGATCA T^GATCA
BcnI 1847 CCCGG CC^[GC]GG
BglI 883 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 619 AGATCT A^GATCT
BmyI 1332 GAGCCC G[AGT]GC[ACT]^C
BsiEI 8 CGGCCG CG[GA][CT]^CG
Bsp1286I 1332 GAGCCC G[AGT]GC[ACT]^C
BsrDI 367 GCAATG GCAATG(2/0)
BstAPI 339 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1409 GGTCACC G^GT[ACGT]ACC
BstPI 1409 GGTCACC G^GT[ACGT]ACC
BstYI 619 AGATCT [GA]^GATC[CT]
DraI 1116 TTTAAA TTT^AAA
DsaI 5 CCACGG C^C[GA][CT]GG
EagI 8 CGGCCG C^GGCCG
EarI 868 CTCTTC CTCTTC(1/4)
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoO65I 1409 GGTCACC G^GT[ACGT]ACC
EcoT22I 1678 ATGCAT ATGCA^T
FauI 39 GCGGG CCCGC(4/6)
FbaI 1467 TGATCA T^GATCA
FspI 471 TGCGCA TGC^GCA
HpaI 556 GTTAAC GTT^AAC
Ksp632I 868 CTCTTC CTCTTC(1/4)
MaeII 1575 ACGT A^CGT
MfeI 1080 CAATTG C^AATTG
MflI 619 AGATCT [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MunI 1080 CAATTG C^AATTG
NciI 1847 CCCGG CC^[GC]GG
NsiI 1678 ATGCAT ATGCA^T
PflMI 713 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1128 GACTC GAGTC(4/5)
SalI 1804 GTCGAC G^TCGAC
ScaI 1811 AGTACT AGT^ACT
SexAI 1062 ACCAGGT A^CC[AT]GGT
SspI 1019 AATATT AAT^ATT
StuI 1643 AGGCCT AGG^CCT
Van91I 713 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 619 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 38 CGCG CG^CG
55 CGCG
AciI 1414 CCGC CCGC(-3/-1)
39 GCGG
AvaII 999 GGACC G^G[AT]CC
1343 GGACC
BbsI 1034 GAAGAC GAAGAC(2/6)
1179 GTCTTC
BpuAI 1034 GAAGAC GAAGAC(2/6)
1179 GTCTTC
BsgI 114 CTGCAC GTGCAG(16/14)
438 CTGCAC
BsmAI 1536 GAGAC GTCTC(1/5)
856 GTCTC
BsmFI 41 GGGAC GGGAC(10/14)
1342 GGGAC
BsmI 1959 GAATGC GAATGC(1/-1)
1897 GCATTC
BsrFI 17 GCCGGC [GA]^CCGG[CT]
28 GCCGGC
BstUI 38 CGCG CG^CG
55 CGCG
BstXI 1059 CCAACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1444 CCAAGATTGTGG
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
28 GCCGGC
DraII 998 AGGACCT [GA]G^G[ACGT]CC[CT]
1342 GGGACCC
EaeI 2 TGGCCA [CT]^GGCC[GA]
8 CGGCCG
EcoO109I 998 AGGACCT [GA]G^G[ACGT]CC[CT]
1342 GGGACCC
HindIII 716 AAGCTT A^AGCTT
1819 AAGCTT
MvnI 38 CGCG CG^CG
55 CGCG
NaeI 17 GCCGGC GCC^GGC
28 GCCGGC
NgoMIV 17 GCCGGC G^CCGGC
28 GCCGGC
PpuMI 998 AGGACCT [GA]G^G[AT]CC[CT]
1342 GGGACCC
PstI 395 CTGCAG CTGCA^G
1976 CTGCAG
TaqI 1805 TCGA T^CGA
1929 TCGA
Tsp45I 188 GTGAC ^GT[GC]AC
1410 GTCAC
TthHB8I 1805 TCGA T^CGA
1929 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
BbvCI BbvI BfaI BpmI BsaJI
BsiYI BslI BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI