Restriction map for pF1KSDB1384
 (Linear) (Six-base) MAPSORT of: KIBB1384.seq  Check: 1188  from: 1  to: 2022



KIBB1384.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1408    2022 
   Size:       1408     614
 
AccI GT'mk_AC

Cuts at:      0    1805    2022 
   Size:       1805     217
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1253    2022 
   Size:       1253     769
 
AleI CACnn'nnGTG

Cuts at:      0      10     647    2022 
   Size:         10     637    1375

  Fragments arranged by size: 

                1375     637      10
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1991    2022 
   Size:       1991      31
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     646     678    2022 
   Size:        646      32    1344

  Fragments arranged by size: 

                1344     646      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     171    1002    1137    1465    2022 
   Size:        171     831     135     328     557

  Fragments arranged by size: 

                 831     557     328     171     135
 
ApoI r'AATT_y

Cuts at:      0     675    1212    1382    1483    1590    2022 
   Size:        675     537     170     101     107     432

  Fragments arranged by size: 

                 675     537     432     170     107     101
 
BanII G_rGCy'C

Cuts at:      0    1336    2022 
   Size:       1336     686
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1041    1172    2022 
   Size:       1041     131     850

  Fragments arranged by size: 

                1041     850     131
 
BbvCI CC'TCA_GC

Cuts at:      0     335    1054    1424    1520    2022 
   Size:        335     719     370      96     502

  Fragments arranged by size: 

                 719     502     370     335      96
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     857    2022 
   Size:        857    1165
 
BclI T'GATC_A

Cuts at:      0    1467    2022 
   Size:       1467     555
 
BfrBI ATG'CAT

Cuts at:      0    1680    2022 
   Size:       1680     342
 
BglI GCCn_nnn'nGGC

Cuts at:      0     889    2022 
   Size:        889    1133
 
BglII A'GATC_T

Cuts at:      0     619    2022 
   Size:        619    1403
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1186    1538    2022 
   Size:       1186     352     484

  Fragments arranged by size: 

                1186     484     352
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     285     536    1202    2022 
   Size:         87     198     251     666     820

  Fragments arranged by size: 

                 820     666     251     198      87
 
Bpu10I CC'TnA_GC

Cuts at:      0     335    1054    1424    1520    2022 
   Size:        335     719     370      96     502

  Fragments arranged by size: 

                 719     502     370     335      96
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     423    2022 
   Size:         99     324    1599

  Fragments arranged by size: 

                1599     324      99
 
BsiEI CG_ry'CG

Cuts at:      0      11    2022 
   Size:         11    2011
 
BsmI GAATG_Cn'

Cuts at:      0    1897    1965    2022 
   Size:       1897      68      57

  Fragments arranged by size: 

                1897      68      57
 
Bsp1286I G_dGCh'C

Cuts at:      0    1336    2022 
   Size:       1336     686
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      23     487     747    1408    2022 
   Size:         23     464     260     661     614

  Fragments arranged by size: 

                 661     614     464     260      23
 
BsrDI GCAATG_nn'

Cuts at:      0     374    2022 
   Size:        374    1648
 
BsrFI r'CCGG_y

Cuts at:      0      17      28    2022 
   Size:         17      11    1994

  Fragments arranged by size: 

                1994      17      11
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     345    2022 
   Size:        345    1677
 
BstEII G'GTnAC_C

Cuts at:      0    1409    2022 
   Size:       1409     613
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1066    1451    2022 
   Size:       1066     385     571

  Fragments arranged by size: 

                1066     571     385
 
BstYI r'GATC_y

Cuts at:      0     619    2022 
   Size:        619    1403
 
BtgI C'CryG_G

Cuts at:      0       5    2022 
   Size:          5    2017
 
BtsI GCAGTG_nn'

Cuts at:      0     404     728    1789    2022 
   Size:        404     324    1061     233

  Fragments arranged by size: 

                1061     404     324     233
 
DraI TTT'AAA

Cuts at:      0    1118    2022 
   Size:       1118     904
 
EaeI y'GGCC_r

Cuts at:      0       2       8    2022 
   Size:          2       6    2014

  Fragments arranged by size: 

                2014       6       2
 
EagI C'GGCC_G

Cuts at:      0       8    2022 
   Size:          8    2014
 
EarI CTCTTCn'nnn_

Cuts at:      0     874    2022 
   Size:        874    1148
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     285     536    1202    1253    2022 
   Size:         87     198     251     666      51     769

  Fragments arranged by size: 

                 769     666     251     198      87      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0     999    1343    2022 
   Size:        999     344     679

  Fragments arranged by size: 

                 999     679     344
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1611    2022 
   Size:       1611     411
 
FspI TGC'GCA

Cuts at:      0     473    2022 
   Size:        473    1549
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     315     347     611     643    1131    1163    2022 
   Size:        315      32     264      32     488      32     859

  Fragments arranged by size: 

                 859     488     315     264      32      32      32
 
HincII GTy'rAC

Cuts at:      0     558    1787    1806    2022 
   Size:        558    1229      19     216

  Fragments arranged by size: 

                1229     558     216      19
 
HindIII A'AGCT_T

Cuts at:      0     716    1819    2022 
   Size:        716    1103     203

  Fragments arranged by size: 

                1103     716     203
 
HpaI GTT'AAC

Cuts at:      0     558    2022 
   Size:        558    1464
 
MfeI C'AATT_G

Cuts at:      0    1080    2022 
   Size:       1080     942
 
MscI TGG'CCA

Cuts at:      0       4    2022 
   Size:          4    2018
 
MslI CAynn'nnrTG

Cuts at:      0      10     647     733     749    1958    2022 
   Size:         10     637      86      16    1209      64

  Fragments arranged by size: 

                1209     637      86      64      16      10
 
NaeI GCC'GGC

Cuts at:      0      19      30    2022 
   Size:         19      11    1992

  Fragments arranged by size: 

                1992      19      11
 
NgoMIV G'CCGG_C

Cuts at:      0      17      28    2022 
   Size:         17      11    1994

  Fragments arranged by size: 

                1994      17      11
 
NsiI A_TGCA'T

Cuts at:      0    1682    2022 
   Size:       1682     340
 
PasI CC'CwG_GG

Cuts at:      0     408    2022 
   Size:        408    1614
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     719    2022 
   Size:        719    1303
 
PpuMI rG'GwC_Cy

Cuts at:      0     999    1343    2022 
   Size:        999     344     679

  Fragments arranged by size: 

                 999     679     344
 
PsiI TTA'TAA

Cuts at:      0     130    2022 
   Size:        130    1892
 
PstI C_TGCA'G

Cuts at:      0     399    1980    2022 
   Size:        399    1581      42

  Fragments arranged by size: 

                1581     399      42
 
SalI G'TCGA_C

Cuts at:      0    1804    2022 
   Size:       1804     218
 
SanDI GG'GwC_CC

Cuts at:      0    1343    2022 
   Size:       1343     679
 
ScaI AGT'ACT

Cuts at:      0    1813    2022 
   Size:       1813     209
 
SexAI A'CCwGG_T

Cuts at:      0    1062    2022 
   Size:       1062     960
 
SfcI C'TryA_G

Cuts at:      0     395     531     537    1976    2022 
   Size:        395     136       6    1439      46

  Fragments arranged by size: 

                1439     395     136      46       6
 
SspI AAT'ATT

Cuts at:      0    1021    2022 
   Size:       1021    1001
 
StuI AGG'CCT

Cuts at:      0    1645    2022 
   Size:       1645     377
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1652    2022 
   Size:       1652     370
 
TatI w'GTAC_w

Cuts at:      0     416    1811    2022 
   Size:        416    1395     211

  Fragments arranged by size: 

                1395     416     211
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      34      66    1850    1882    2022 
   Size:         34      32    1784      32     140

  Fragments arranged by size: 

                1784     140      34      32      32
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AlfI     AloI    AlwNI     ApoI
    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BmrI     BpmI   Bpu10I     BsgI    BsiEI     BsmI Bsp1286I    BspMI
    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI
     DraI     EaeI     EagI     EarI  Eco57MI EcoO109I     FalI     FspI
    Hin4I   HincII  HindIII     HpaI     MfeI     MscI     MslI     NaeI
   NgoMIV     NsiI     PasI    PflMI    PpuMI     PsiI     PstI     SalI
    SanDI     ScaI    SexAI     SfcI     SspI     StuI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BcgI     BlpI Bme1580I    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI   DraIII     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI    FspAI    HaeII     KasI     KpnI     MluI     MmeI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NruI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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