Restriction map for pF1KE2504
 (Circular) (Six-base) MAPSORT of: pF1KE2504.seq  Check: 6196  from: 1  to: 6155



pF1KE2504  6155 bp

 With 182 enzymes: SgfI * 

                        February 16, 2012 13:36  ..

AatII G_ACGT'C

Cuts at:   1052    1493    1052 
   Size:        441    5714
 
AccI GT'mk_AC

Cuts at:   3008    3193    3008 
   Size:        185    5970
 
Acc65I G'GTAC_C

Cuts at:    260     578    3171     260 
   Size:        318    2593    3244

  Fragments arranged by size: 

                3244    2593     318
 
AclI AA'CG_TT

Cuts at:   5995    5995 
   Size:       6155
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    642     712     910    1244    1498    1749    2170    2552    2774 
   Size:         70     198     334     254     251     421     382     222

Cuts at:   2774    2923    2966    3124    3980    4412    5159    5427     642 
   Size:        149      43     158     856     432     747     268    1370

  Fragments arranged by size: 

                1370     856     747     432     421     382     334     268
                 254     251     222     198     158     149      70      43
            
 
AflIII A'CryG_T

Cuts at:   2774    2891    3507    4611    5588    2774 
   Size:        117     616    1104     977    3341

  Fragments arranged by size: 

                3341    1104     977     616     117
 
AgeI A'CCGG_T

Cuts at:   4543    4543 
   Size:       6155
 
AlwNI CAG_nnn'CTG

Cuts at:   1573    1609    1830    1871    2295    3460    5027    1573 
   Size:         36     221      41     424    1165    1567    2701

  Fragments arranged by size: 

                2701    1567    1165     424     221      41      36
 
ApaLI G'TGCA_C

Cuts at:   4925    4925 
   Size:       6155
 
ApoI r'AATT_y

Cuts at:   1120    1995    3124    3159    5508    5764    1120 
   Size:        875    1129      35    2349     256    1511

  Fragments arranged by size: 

                2349    1511    1129     875     256      35
 
AscI GG'CGCG_CC

Cuts at:    159     159 
   Size:       6155
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6155
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6155
 
AvaI C'yCGr_G

Cuts at:    628    1002    1300    1351    3175     628 
   Size:        374     298      51    1824    3608

  Fragments arranged by size: 

                3608    1824     374     298      51
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    244     277     244 
   Size:         33    6122
 
BamHI G'GATC_C

Cuts at:   1707    2469    2720    3180    1707 
   Size:        762     251     460    4682

  Fragments arranged by size: 

                4682     762     460     251
 
BanI G'GyrC_C

Cuts at:    260     302     578     976    2086    3171    3585    3833    3868 
   Size:         42     276     398    1110    1085     414     248      35

Cuts at:   3868     260 
   Size:       2547

  Fragments arranged by size: 

                2547    1110    1085     414     398     276     248      42
                  35
 
BanII G_rGCy'C

Cuts at:   3169    4199    3169 
   Size:       1030    5125
 
BbeI G_GCGC'C

Cuts at:   3589    3837    3589 
   Size:        248    5907
 
BbsI GAAGACnn'nnnn_

Cuts at:    110     373     436    1008    1755    2728     110 
   Size:        263      63     572     747     973    3537

  Fragments arranged by size: 

                3537     973     747     572     263      63
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    619     653     954     988     619 
   Size:         34     301      34    5786

  Fragments arranged by size: 

                5786     301      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2018    4053    4814    6155    2018 
   Size:       2035     761    1341    2018

  Fragments arranged by size: 

                2035    2018    1341     761
 
BclI T'GATC_A

Cuts at:   3675    3675 
   Size:       6155
 
BglI GCCn_nnn'nGGC

Cuts at:    823    1201    1381     823 
   Size:        378     180    5597

  Fragments arranged by size: 

                5597     378     180
 
BglII A'GATC_T

Cuts at:   3670    5707    3670 
   Size:       2037    4118
 
BlpI GC'TnA_GC

Cuts at:   2209    3267    2209 
   Size:       1058    5097
 
Bme1580I G_kGCm'C

Cuts at:    981    3780    3873    4929     981 
   Size:       2799      93    1056    2207

  Fragments arranged by size: 

                2799    2207    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1052    3367    3448    3783    5927    1052 
   Size:       2315      81     335    2144    1280

  Fragments arranged by size: 

                2315    2144    1280     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1017    4522    1017 
   Size:       3505    2650
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    401    1468    1765    1852    2050     401 
   Size:       1067     297      87     198    4506

  Fragments arranged by size: 

                4506    1067     297     198      87
 
Bpu10I CC'TnA_GC

Cuts at:   2440    3092    2440 
   Size:        652    5503
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    634    1126    3331    4210    4702    5000     634 
   Size:        492    2205     879     492     298    1789

  Fragments arranged by size: 

                2205    1789     879     492     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2814    2840    5859    2814 
   Size:         26    3019    3110

  Fragments arranged by size: 

                3110    3019      26
 
BsaAI yAC'GTr

Cuts at:    479    3508     479 
   Size:       3029    3126
 
BsaBI GATnn'nnATC

Cuts at:      6     214    3669    5706       6 
   Size:        208    3455    2037     455

  Fragments arranged by size: 

                3455    2037     455     208
 
BsaHI Gr'CG_yC

Cuts at:   1049    1490    3586    3834    4536    6032    1049 
   Size:        441    2096     248     702    1496    1172

  Fragments arranged by size: 

                2096    1496    1172     702     441     248
 
BsaWI w'CCGG_w

Cuts at:    206    1094    3344    3865    4543    4817    4964    5375     206 
   Size:        888    2250     521     678     274     147     411     986

  Fragments arranged by size: 

                2250     986     888     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    253     283    1688    1718    2794    2824     253 
   Size:         30    1405      30    1076      30    3584

  Fragments arranged by size: 

                3584    1405    1076      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    398     625    1242    1709     398 
   Size:        227     617     467    4844

  Fragments arranged by size: 

                4844     617     467     227
 
BseYI C'CCAG_C

Cuts at:    615    2716    3580    4338    4915    5411     615 
   Size:       2101     864     758     577     496    1359

  Fragments arranged by size: 

                2101    1359     864     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    244    1023    2401     244 
   Size:        779    1378    3998

  Fragments arranged by size: 

                3998    1378     779
 
BsiEI CG_ry'CG

Cuts at:    104    3697    3743    4951     104 
   Size:       3593      46    1208    1308

  Fragments arranged by size: 

                3593    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1946    3169    3947    4137    4929    1946 
   Size:       1223     778     190     792    3172

  Fragments arranged by size: 

                3172    1223     792     778     190
 
BsmI GAATG_Cn'

Cuts at:   1003    2644    1003 
   Size:       1641    4514
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1497    2843    1497 
   Size:       1346    4809
 
Bsp1286I G_dGCh'C

Cuts at:    981    1946    3169    3780    3873    3947    4137    4199    4929 
   Size:        965    1223     611      93      74     190      62     730

Cuts at:   4929     981 
   Size:       2207

  Fragments arranged by size: 

                2207    1223     965     730     611     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:    206    5375     206 
   Size:       5169     986
 
BspHI T'CATG_A

Cuts at:   1949    5331    1949 
   Size:       3382    2773
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2417    3205    3419    3721    4102    2417 
   Size:        788     214     302     381    4470

  Fragments arranged by size: 

                4470     788     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     155    2358    4447    4501    5985    6150      50 
   Size:        105    2203    2089      54    1484     165      55

  Fragments arranged by size: 

                2203    2089    1484     165     105      55      54
 
BsrDI GCAATG_nn'

Cuts at:   4067    4067 
   Size:       6155
 
BsrFI r'CCGG_y

Cuts at:   4153    4334    4543    4153 
   Size:        181     209    5765

  Fragments arranged by size: 

                5765     209     181
 
BssHII G'CGCG_C

Cuts at:    159     159 
   Size:       6155
 
BssSI C'ACGA_G

Cuts at:   4426    4784    4426 
   Size:        358    5797
 
BstAPI GCAn_nnn'nTGC

Cuts at:    176     457     921    3532     176 
   Size:        281     464    2611    2799

  Fragments arranged by size: 

                2799    2611     464     281
 
BstBI TT'CG_AA

Cuts at:   4516    4516 
   Size:       6155
 
BstEII G'GTnAC_C

Cuts at:   1107    1107 
   Size:       6155
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1071    2338    1071 
   Size:       1267    4888
 
BstYI r'GATC_y

Cuts at:      1    1289    1707    2222    2291    2298    2469    2720    2789 
   Size:       1288     418     515      69       7     171     251      69

Cuts at:   2789    3180    3647    3670    4005    4251    5263    5349    5361 
   Size:        391     467      23     335     246    1012      86      12

Cuts at:   5361    5707       1 
   Size:        346     449

  Fragments arranged by size: 

                1288    1012     515     467     449     418     391     346
                 335     251     246     171      86      69      69      23
                  12       7
 
BtgI C'CryG_G

Cuts at:    107    1199    2162     107 
   Size:       1092     963    4100

  Fragments arranged by size: 

                4100    1092     963
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    752    1040    4105    4286    4449     752 
   Size:        288    3065     181     163    2458

  Fragments arranged by size: 

                3065    2458     288     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1052    2066    3398    1052 
   Size:       1014    1332    3809

  Fragments arranged by size: 

                3809    1332    1014
 
ClaI AT'CG_AT

Cuts at:   2477    2609    2477 
   Size:        132    6023
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   2694    2729    2694 
   Size:         35    6120
 
DraI TTT'AAA

Cuts at:   3154    3154 
   Size:       6155
 
DrdI GACnn_nn'nnGTC

Cuts at:   2602    3861    4719    2602 
   Size:       1259     858    4038

  Fragments arranged by size: 

                4038    1259     858
 
EaeI y'GGCC_r

Cuts at:    212    1205    1373    2043    3740    3914    4305    4332    5677 
   Size:        993     168     670    1697     174     391      27    1345

Cuts at:   5677     212 
   Size:        690

  Fragments arranged by size: 

                1697    1345     993     690     670     391     174     168
                  27
 
EagI C'GGCC_G

Cuts at:   3740    3740 
   Size:       6155
 
EarI CTCTTCn'nnn_

Cuts at:   2048    4178    4388    2048 
   Size:       2130     210    3815

  Fragments arranged by size: 

                3815    2130     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127    1205    1485    4363    4673    4819    5965     127 
   Size:       1078     280    2878     310     146    1146     317

  Fragments arranged by size: 

                2878    1146    1078     317     310     280     146
 
EcoICRI GAG'CTC

Cuts at:   3167    3167 
   Size:       6155
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    401     642     712     910    1244    1468    1498    1749    1765 
   Size:        241      70     198     334     224      30     251      16

Cuts at:   1765    1852    2050    2170    2552    2774    2923    2966    3124 
   Size:         87     198     120     382     222     149      43     158

Cuts at:   3124    3980    4412    5159    5427     401 
   Size:        856     432     747     268    1129

  Fragments arranged by size: 

                1129     856     747     432     382     334     268     251
                 241     224     222     198     198     158     149     120
                  87      70      43      30      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2248    2728    2248 
   Size:        480    5675
 
EcoO109I rG'GnC_Cy

Cuts at:   1868    2118    3294    1868 
   Size:        250    1176    4729

  Fragments arranged by size: 

                4729    1176     250
 
EcoRI G'AATT_C

Cuts at:   1120    3159    1120 
   Size:       2039    4116
 
EcoRV GAT'ATC

Cuts at:   2613    2613 
   Size:       6155
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2151    2214    2899    4174    2151 
   Size:         63     685    1275    4132

  Fragments arranged by size: 

                4132    1275     685      63
 
FspI TGC'GCA

Cuts at:   1736    3936    1736 
   Size:       2200    3955
 
HaeII r_GCGC'y

Cuts at:   1196    2761    3589    3837    4859    5838    1196 
   Size:       1565     828     248    1022     979    1513

  Fragments arranged by size: 

                1565    1513    1022     979     828     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    313     345    1281    1313    2064    2096    2283    2315    2794 
   Size:         32     936      32     751      32     187      32     479

Cuts at:   2794    2826    3997    4027    4029    4059     313 
   Size:         32    1171      30       2      30    2409

  Fragments arranged by size: 

                2409    1171     936     751     479     187      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1364    2484    2520    3194    1364 
   Size:       1120      36     674    4325

  Fragments arranged by size: 

                4325    1120     674      36
 
HindIII A'AGCT_T

Cuts at:    805     805 
   Size:       6155
 
KasI G'GCGC_C

Cuts at:   3585    3833    3585 
   Size:        248    5907
 
KpnI G_GTAC'C

Cuts at:    264     582    3175     264 
   Size:        318    2593    3244

  Fragments arranged by size: 

                3244    2593     318
 
MluI A'CGCG_T

Cuts at:   5588    5588 
   Size:       6155
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    491    1304    4826    5010     491 
   Size:        813    3522     184    1636

  Fragments arranged by size: 

                3522    1636     813     184
 
MscI TGG'CCA

Cuts at:    214    2045    3916     214 
   Size:       1831    1871    2453

  Fragments arranged by size: 

                2453    1871    1831
 
MslI CAynn'nnrTG

Cuts at:    220    2128    2422    2512    4144    4271     220 
   Size:       1908     294      90    1632     127    2104

  Fragments arranged by size: 

                2104    1908    1632     294     127      90
 
MspA1I CmG'CkG

Cuts at:    373     575    1011    1702    3266    3580    3940    4953    5198 
   Size:        202     436     691    1564     314     360    1013     245

Cuts at:   5198     373 
   Size:       1330

  Fragments arranged by size: 

                1564    1330    1013     691     436     360     314     245
                 202
 
NaeI GCC'GGC

Cuts at:   4336    4336 
   Size:       6155
 
NarI GG'CG_CC

Cuts at:   3586    3834    3586 
   Size:        248    5907
 
NcoI C'CATG_G

Cuts at:    107    2162     107 
   Size:       2055    4100
 
NgoMIV G'CCGG_C

Cuts at:   4334    4334 
   Size:       6155
 
NspI r_CATG'y

Cuts at:   1741    2778    2895    3208    3708    4615    1741 
   Size:       1037     117     313     500     907    3281

  Fragments arranged by size: 

                3281    1037     907     500     313     117
 
PasI CC'CwG_GG

Cuts at:   2730    2730 
   Size:       6155
 
PciI A'CATG_T

Cuts at:   2774    2891    4611    2774 
   Size:        117    1720    4318

  Fragments arranged by size: 

                4318    1720     117
 
PfoI T'CCnGG_A

Cuts at:   1312    1484    1742    2294    2698    1312 
   Size:        172     258     552     404    4769

  Fragments arranged by size: 

                4769     552     404     258     172
 
PmeI GTTT'AAAC

Cuts at:   3154    3154 
   Size:       6155
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5294    5326    5294 
   Size:         32    6123
 
PpuMI rG'GwC_Cy

Cuts at:   1868    2118    1868 
   Size:        250    5905
 
PstI C_TGCA'G

Cuts at:   1826    3202    3887    1826 
   Size:       1376     685    4094

  Fragments arranged by size: 

                4094    1376     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6155
 
PvuII CAG'CTG

Cuts at:   1011    1702    3580    3940    1011 
   Size:        691    1878     360    3226

  Fragments arranged by size: 

                3226    1878     691     360
 
RsrII CG'GwC_CG

Cuts at:   4350    4350 
   Size:       6155
 
SacI G_AGCT'C

Cuts at:   3169    3169 
   Size:       6155
 
SalI G'TCGA_C

Cuts at:   3192    3192 
   Size:       6155
 
SapI GCTCTTCn'nnn_

Cuts at:   4178    4388    4178 
   Size:        210    5945
 
SbfI CC_TGCA'GG

Cuts at:   3202    3202 
   Size:       6155
 
ScaI AGT'ACT

Cuts at:   5672    5672 
   Size:       6155
 
SfcI C'TryA_G

Cuts at:     33     671    1822    2430    3198    3883    4876    5067      33 
   Size:        638    1151     608     768     685     993     191    1121

  Fragments arranged by size: 

                1151    1121     993     768     685     638     608     191
            
 
SfoI GGC'GCC

Cuts at:   3587    3835    3587 
   Size:        248    5907
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6155
 
SmaI CCC'GGG

Cuts at:   3177    3177 
   Size:       6155
 
SmlI C'TyrA_G

Cuts at:    649    1141    3310    4225    4717    4979     649 
   Size:        492    2169     915     492     262    1825

  Fragments arranged by size: 

                2169    1825     915     492     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6155
 
SphI G_CATG'C

Cuts at:   3208    3708    3208 
   Size:        500    5655
 
StyI C'CwwG_G

Cuts at:    107     194    1546    1776    1925    2162    2198    2387    2883 
   Size:         87    1352     230     149     237      36     189     496

Cuts at:   2883    2933    3289    3643     107 
   Size:         50     356     354    2619

  Fragments arranged by size: 

                2619    1352     496     356     354     237     230     189
                 149      87      50      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    427     965    1104    3738    3871    4330    4539     427 
   Size:        538     139    2634     133     459     209    2043

  Fragments arranged by size: 

                2634    2043     538     459     209     139     133
 
TatI w'GTAC_w

Cuts at:    632    3071    5670     632 
   Size:       2439    2599    1117

  Fragments arranged by size: 

                2599    2439    1117
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1525    1557    1688    1720    1992    2024    5672    5704    1525 
   Size:         32     131      32     272      32    3648      32    1976

  Fragments arranged by size: 

                3648    1976     272     131      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   2806    3952    2806 
   Size:       1146    5009
 
XbaI T'CTAG_A

Cuts at:   3186    3186 
   Size:       6155
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    409    1197    1410    1779    3650     409 
   Size:        788     213     369    1871    2914

  Fragments arranged by size: 

                2914    1871     788     369     213
 
XmaI C'CCGG_G

Cuts at:   3175    3175 
   Size:       6155
 
XmnI GAAnn'nnTTC

Cuts at:   1762    1762 
   Size:       6155
 
ZraI GAC'GTC

Cuts at:   1050    1491    1050 
   Size:        441    5714
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
    AvrII    BbvCI    BfrBI    BmgBI     BmtI    BsiWI    BsrGI  BstZ17I
   Bsu36I   DraIII     FseI    FspAI     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI    PflMI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
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