Restriction map for pF1KSDB0185
##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        ApaI        AseI        AsnI        Asp718I     
AspI        AvrII       BciVI       BfaI        BfrI        
BlnI        BsiWI       Bsp1407I    BspDI       BsrGI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        DrdI        EcoRI       EcoRV       EcoT22I     
FseI        HindIII     HpaI        KpnI        KspI        
MaeI        MfeI        MluI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AcsI         1613  AAATTT               [GA]^AATT[CT]
AhdI         4441  GACGAGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4566  GTGCAC               G^TGCAC
Aor51HI      2191  AGCGCT               AGC^GCT
ApaLI        4566  GTGCAC               G^TGCAC
ApoI         1613  AAATTT               [GA]^AATT[CT]
AscI         2414  GGCGCGCC             GG^CGCGCC
AspEI        4441  GACGAGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         110  GGATCC               G^GATCC
BbeI         4475  GGCGCC               GGCGC^C
BclI          305  TGATCA               T^GATCA
BglII        4871  AGATCT               A^GATCT
BsmBI        3631  GAGACG               CGTCTC(1/5)
BsmI         3151  GCATTC               GAATGC(1/-1)
BspHI         965  TCATGA               T^CATGA
BspLU11I      488  ACATGT               A^CATGT
BssHII       2415  GCGCGC               G^CGCGC
DraIII       2161  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     4441  GACGAGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2191  AGCGCT               AGC^GCT
FbaI          305  TGATCA               T^GATCA
KasI         4475  GGCGCC               G^GCGCC
NaeI         2092  GCCGGC               GCC^GGC
NarI         4475  GGCGCC               GG^CGCC
NgoMIV       2092  GCCGGC               G^CCGGC
PshAI        2628  GACCTGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          965  TCATGA               T^CATGA
SbfI         3075  CCTGCAGG             CCTGCA^GG
ScaI         1376  AGTACT               AGT^ACT
SfoI         4475  GGCGCC               GGC^GCC
SphI          645  GCATGC               GCATG^C
Sse8387I     3075  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccIII       1637  TCCGGA               T^CCGGA
             3842  TCCGGA              
AcyI         1912  GACGCC               G[GA]^CG[CT]C
             4475  GGCGCC              
AflIII        488  ACATGT               A^C[GA][CT]GT
             3566  ACGTGT              
AocI         3761  CCTCAGG              CC^T[ACGT]AGG
             3892  CCTGAGG             
BsaBI         111  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1413  GATCAAGATC          
BsaHI        1912  GACGCC               G[GA]^CG[CT]C
             4475  GGCGCC              
BsaWI        1637  TCCGGA               [AT]^CCGG[AT]
             3842  TCCGGA              
BseAI        1637  TCCGGA               T^CCGGA
             3842  TCCGGA              
BspEI        1637  TCCGGA               T^CCGGA
             3842  TCCGGA              
BsrBI        1821  GAGCGG               CCGCTC(-3/-3)
             2887  GAGCGG              
Bsu36I       3761  CCTCAGG              CC^T[ACGT]AGG
             3892  CCTGAGG             
EagI         1466  CGGCCG               C^GGCCG
             3735  CGGCCG              
EclXI        1466  CGGCCG               C^GGCCG
             3735  CGGCCG              
Eco52I       1466  CGGCCG               C^GGCCG
             3735  CGGCCG              
Eco81I       3761  CCTCAGG              CC^T[ACGT]AGG
             3892  CCTGAGG             
Hin1I        1912  GACGCC               G[GA]^CG[CT]C
             4475  GGCGCC              
HincII       1147  GTCAAC               GT[CT]^[GA]AC
             2938  GTCAAC              
HindII       1147  GTCAAC               GT[CT]^[GA]AC
             2938  GTCAAC              
MamI          111  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1413  GATCAAGATC          
MroI         1637  TCCGGA               T^CCGGA
             3842  TCCGGA              
SacI         2671  GAGCTC               GAGCT^C
             3979  GAGCTC              
SexAI        1225  ACCTGGT              A^CC[AT]GGT
             1400  ACCTGGT             
SmlI         2074  CTCAAG               C^T[CT][GA]AG
             4627  CTCAAG              
StuI         3320  AGGCCT               AGG^CCT
             4709  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       Asp700I     AspHI       AvaI        AvaII       
AviII       BalI        BanI        BanII       BbrPI       
BbsI        BbvCI       BbvI        BcgI        BcnI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrDI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PmaCI       PmlI        PpuMI       
PstI        PvuII       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfiI        
SmaI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI        
XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com