Restriction map for pF1KSDB0058
 (Linear) (Six-base) MAPSORT of: KIBB0058.seq  Check: 8144  from: 1  to: 3303



KIBB0058.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AccI GT'mk_AC

Cuts at:      0    1589    2399    3101    3303 
   Size:       1589     810     702     202

  Fragments arranged by size: 

                1589     810     702     202
 
Acc65I G'GTAC_C

Cuts at:      0    2072    3303 
   Size:       2072    1231
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     542     811    1123    1461    1814    3256    3303 
   Size:        274     268     269     312     338     353    1442      47

  Fragments arranged by size: 

                1442     353     338     312     274     269     268      47
            
 
AflIII A'CryG_T

Cuts at:      0    2239    3303 
   Size:       2239    1064
 
AleI CACnn'nnGTG

Cuts at:      0    1045    3303 
   Size:       1045    2258
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3011    3199    3303 
   Size:       3011     188     104

  Fragments arranged by size: 

                3011     188     104
 
AlwNI CAG_nnn'CTG

Cuts at:      0     453    1579    2531    3246    3303 
   Size:        453    1126     952     715      57

  Fragments arranged by size: 

                1126     952     715     453      57
 
ApaI G_GGCC'C

Cuts at:      0     123    1099    3303 
   Size:        123     976    2204

  Fragments arranged by size: 

                2204     976     123
 
ApoI r'AATT_y

Cuts at:      0     536    3303 
   Size:        536    2767
 
AvaI C'yCGr_G

Cuts at:      0      95     377     598     983    1517    1559    2076    2775 
   Size:         95     282     221     385     534      42     517     699

Cuts at:   2775    3131    3303 
   Size:        356     172

  Fragments arranged by size: 

                 699     534     517     385     356     282     221     172
                  95      42
 
BamHI G'GATC_C

Cuts at:      0    1196    3303 
   Size:       1196    2107
 
BanI G'GyrC_C

Cuts at:      0     847     925    1392    2072    2445    2809    3303 
   Size:        847      78     467     680     373     364     494

  Fragments arranged by size: 

                 847     680     494     467     373     364      78
 
BanII G_rGCy'C

Cuts at:      0     123     326    1099    1571    2009    2083    2555    2721 
   Size:        123     203     773     472     438      74     472     166

Cuts at:   2721    3168    3303 
   Size:        447     135

  Fragments arranged by size: 

                 773     472     472     447     438     203     166     135
                 123      74
 
BbeI G_GCGC'C

Cuts at:      0     851     929    1396    3303 
   Size:        851      78     467    1907

  Fragments arranged by size: 

                1907     851     467      78
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     530    1192    1647    2577    3303 
   Size:        530     662     455     930     726

  Fragments arranged by size: 

                 930     726     662     530     455
 
BbvCI CC'TCA_GC

Cuts at:      0      66     347    3303 
   Size:         66     281    2956

  Fragments arranged by size: 

                2956     281      66
 
BclI T'GATC_A

Cuts at:      0     185    1826    2694    2906    3222    3303 
   Size:        185    1641     868     212     316      81

  Fragments arranged by size: 

                1641     868     316     212     185      81
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2299    3303 
   Size:       2299    1004
 
BglII A'GATC_T

Cuts at:      0    2406    3303 
   Size:       2406     897
 
BlpI GC'TnA_GC

Cuts at:      0     151    3303 
   Size:        151    3152
 
Bme1580I G_kGCm'C

Cuts at:      0     123    1099    1634    2164    2360    2465    3303 
   Size:        123     976     535     530     196     105     838

  Fragments arranged by size: 

                 976     838     535     530     196     123     105
 
BmgBI CAC'GTC

Cuts at:      0     423    2240    3303 
   Size:        423    1817    1063

  Fragments arranged by size: 

                1817    1063     423
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     126    1202    1610    1635    3303 
   Size:        126    1076     408      25    1668

  Fragments arranged by size: 

                1668    1076     408     126      25
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2105    2303    2735    2748    3303 
   Size:       2105     198     432      13     555

  Fragments arranged by size: 

                2105     555     432     198      13
 
Bpu10I CC'TnA_GC

Cuts at:      0      66     347    3303 
   Size:         66     281    2956

  Fragments arranged by size: 

                2956     281      66
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289    3303 
   Size:        289    3014
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     214    1744    2766    3085    3303 
   Size:        214    1530    1022     319     218

  Fragments arranged by size: 

                1530    1022     319     218     214
 
BsaBI GATnn'nnATC

Cuts at:      0    1012    1201    1645    3303 
   Size:       1012     189     444    1658

  Fragments arranged by size: 

                1658    1012     444     189
 
BsaHI Gr'CG_yC

Cuts at:      0     437     665     848     926    1339    1393    2039    3303 
   Size:        437     228     183      78     413      54     646    1264

  Fragments arranged by size: 

                1264     646     437     413     228     183      78      54
            
 
BsaWI w'CCGG_w

Cuts at:      0    1772    1987    3285    3303 
   Size:       1772     215    1298      18

  Fragments arranged by size: 

                1772    1298     215      18
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     623     653    2065    2095    2339    2369    2414    2444 
   Size:        623      30    1412      30     244      30      45      30

Cuts at:   2444    2746    2776    3303 
   Size:        302      30     527

  Fragments arranged by size: 

                1412     623     527     302     244      45      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     364    1303    2247    2614    2993    3303 
   Size:        364     939     944     367     379     310

  Fragments arranged by size: 

                 944     939     379     367     364     310
 
BseYI C'CCAG_C

Cuts at:      0     264     931    1348    2746    3186    3303 
   Size:        264     667     417    1398     440     117

  Fragments arranged by size: 

                1398     667     440     417     264     117
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2980    3303 
   Size:       2980     323
 
BsiHKAI G_wGCw'C

Cuts at:      0    1112    1333    1468    1706    2009    2555    3303 
   Size:       1112     221     135     238     303     546     748

  Fragments arranged by size: 

                1112     748     546     303     238     221     135
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1556    2715    3303 
   Size:       1556    1159     588

  Fragments arranged by size: 

                1556    1159     588
 
Bsp1286I G_dGCh'C

Cuts at:      0     123     326    1099    1112    1333    1468    1571    1634 
   Size:        123     203     773      13     221     135     103      63

Cuts at:   1634    1706    2009    2083    2164    2360    2465    2555    2721 
   Size:         72     303      74      81     196     105      90     166

Cuts at:   2721    3168    3303 
   Size:        447     135

  Fragments arranged by size: 

                 773     447     303     221     203     196     166     135
                 135     123     105     103      90      81      74      72
                  63      13
 
BspEI T'CCGG_A

Cuts at:      0    1772    1987    3285    3303 
   Size:       1772     215    1298      18

  Fragments arranged by size: 

                1772    1298     215      18
 
BspHI T'CATG_A

Cuts at:      0     977    2519    3303 
   Size:        977    1542     784

  Fragments arranged by size: 

                1542     977     784
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1875    2442    2517    3303 
   Size:       1875     567      75     786

  Fragments arranged by size: 

                1875     786     567      75
 
BsrBI CCG'CTC

Cuts at:      0    1727    3303 
   Size:       1727    1576
 
BsrDI GCAATG_nn'

Cuts at:      0     755    3303 
   Size:        755    2548
 
BsrFI r'CCGG_y

Cuts at:      0    1262    3303 
   Size:       1262    2041
 
BsrGI T'GTAC_A

Cuts at:      0     860    3303 
   Size:        860    2443
 
BssSI C'ACGA_G

Cuts at:      0     406    1815    2410    3303 
   Size:        406    1409     595     893

  Fragments arranged by size: 

                1409     893     595     406
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1533    3303 
   Size:       1533    1770
 
BstEII G'GTnAC_C

Cuts at:      0    2920    3303 
   Size:       2920     383
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1213    2091    3303 
   Size:       1213     878    1212

  Fragments arranged by size: 

                1213    1212     878
 
BstYI r'GATC_y

Cuts at:      0     821    1007    1196    1447    1640    1769    2406    2963 
   Size:        821     186     189     251     193     129     637     557

Cuts at:   2963    3303 
   Size:        340

  Fragments arranged by size: 

                 821     637     557     340     251     193     189     186
                 129
 
BstZ17I GTA'TAC

Cuts at:      0    2400    3303 
   Size:       2400     903
 
Bsu36I CC'TnA_GG

Cuts at:      0     485    1805    3303 
   Size:        485    1320    1498

  Fragments arranged by size: 

                1498    1320     485
 
BtgI C'CryG_G

Cuts at:      0     259     830    1583    1745    2030    2465    2867    3259 
   Size:        259     571     753     162     285     435     402     392

Cuts at:   3259    3303 
   Size:         44

  Fragments arranged by size: 

                 753     571     435     402     392     285     259     162
                  44
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1014    1764    2549    3303 
   Size:       1014     750     785     754

  Fragments arranged by size: 

                1014     785     754     750
 
BtsI GCAGTG_nn'

Cuts at:      0     668    1298    3303 
   Size:        668     630    2005

  Fragments arranged by size: 

                2005     668     630
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     177     212    1899    1934    3303 
   Size:        177      35    1687      35    1369

  Fragments arranged by size: 

                1687    1369     177      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2928    3303 
   Size:       2928     375
 
EaeI y'GGCC_r

Cuts at:      0       5     542     653     836    1235    2054    2274    2414 
   Size:          5     537     111     183     399     819     220     140

Cuts at:   2414    3303 
   Size:        889

  Fragments arranged by size: 

                 889     819     537     399     220     183     140     111
                   5
 
EarI CTCTTCn'nnn_

Cuts at:      0     568    1128    1480    2588    3223    3303 
   Size:        568     560     352    1108     635      80

  Fragments arranged by size: 

                1108     635     568     560     352      80
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1852    3303 
   Size:       1852    1451
 
EcoICRI GAG'CTC

Cuts at:      0    2007    2553    3303 
   Size:       2007     546     750

  Fragments arranged by size: 

                2007     750     546
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     542     811    1123    1461    1814    2105    2303 
   Size:        274     268     269     312     338     353     291     198

Cuts at:   2303    2735    2748    3256    3303 
   Size:        432      13     508      47

  Fragments arranged by size: 

                 508     432     353     338     312     291     274     269
                 268     198      47      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2481    3303 
   Size:       2481     822
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1095    1096    1809    2613    3122    3303 
   Size:       1095       1     713     804     509     181

  Fragments arranged by size: 

                1095     804     713     509     181       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      58     816    3303 
   Size:         58     758    2487

  Fragments arranged by size: 

                2487     758      58
 
HaeII r_GCGC'y

Cuts at:      0     851     929    1396    3303 
   Size:        851      78     467    1907

  Fragments arranged by size: 

                1907     851     467      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     177     209     623     655    1013    1045    1555    1587 
   Size:        177      32     414      32     358      32     510      32

Cuts at:   1587    1841    1873    2372    2404    2537    2569    2679    2711 
   Size:        254      32     499      32     133      32     110      32

Cuts at:   2711    2746    2778    2948    2980    3303 
   Size:         35      32     170      32     323

  Fragments arranged by size: 

                 510     499     414     358     323     254     177     170
                 133     110      35      32      32      32      32      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     222     894    3303 
   Size:        222     672    2409

  Fragments arranged by size: 

                2409     672     222
 
KasI G'GCGC_C

Cuts at:      0     847     925    1392    3303 
   Size:        847      78     467    1911

  Fragments arranged by size: 

                1911     847     467      78
 
KpnI G_GTAC'C

Cuts at:      0    2076    3303 
   Size:       2076    1227
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1857    2024    3303 
   Size:       1857     167    1279

  Fragments arranged by size: 

                1857    1279     167
 
MscI TGG'CCA

Cuts at:      0     544    2056    2276    2416    3303 
   Size:        544    1512     220     140     887

  Fragments arranged by size: 

                1512     887     544     220     140
 
MslI CAynn'nnrTG

Cuts at:      0     998    1045    2089    2671    2956    3181    3298    3303 
   Size:        998      47    1044     582     285     225     117       5

  Fragments arranged by size: 

                1044     998     582     285     225     117      47       5
            
 
MspA1I CmG'CkG

Cuts at:      0     183    1291    1325    2692    3303 
   Size:        183    1108      34    1367     611

  Fragments arranged by size: 

                1367    1108     611     183      34
 
NarI GG'CG_CC

Cuts at:      0     848     926    1393    3303 
   Size:        848      78     467    1910

  Fragments arranged by size: 

                1910     848     467      78
 
NcoI C'CATG_G

Cuts at:      0     830    1583    1745    2465    2867    3303 
   Size:        830     753     162     720     402     436

  Fragments arranged by size: 

                 830     753     720     436     402     162
 
NdeI CA'TA_TG

Cuts at:      0    3276    3303 
   Size:       3276      27
 
NspI r_CATG'y

Cuts at:      0    1512    1536    1998    2571    2955    3108    3303 
   Size:       1512      24     462     573     384     153     195

  Fragments arranged by size: 

                1512     573     462     384     195     153      24
 
PasI CC'CwG_GG

Cuts at:      0    1254    3303 
   Size:       1254    2049
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     162    1502    3303 
   Size:        162    1340    1801

  Fragments arranged by size: 

                1801    1340     162
 
PfoI T'CCnGG_A

Cuts at:      0    2121    3303 
   Size:       2121    1182
 
PpuMI rG'GwC_Cy

Cuts at:      0    2613    3122    3303 
   Size:       2613     509     181

  Fragments arranged by size: 

                2613     509     181
 
PspOMI G'GGCC_C

Cuts at:      0     119    1095    3303 
   Size:        119     976    2208

  Fragments arranged by size: 

                2208     976     119
 
PstI C_TGCA'G

Cuts at:      0     134    2316    3303 
   Size:        134    2182     987

  Fragments arranged by size: 

                2182     987     134
 
PvuII CAG'CTG

Cuts at:      0     183    2692    3303 
   Size:        183    2509     611

  Fragments arranged by size: 

                2509     611     183
 
SacI G_AGCT'C

Cuts at:      0    2009    2555    3303 
   Size:       2009     546     748

  Fragments arranged by size: 

                2009     748     546
 
ScaI AGT'ACT

Cuts at:      0     919    2653    3303 
   Size:        919    1734     650

  Fragments arranged by size: 

                1734     919     650
 
SexAI A'CCwGG_T

Cuts at:      0    2756    3303 
   Size:       2756     547
 
SfcI C'TryA_G

Cuts at:      0     130    2312    3303 
   Size:        130    2182     991

  Fragments arranged by size: 

                2182     991     130
 
SfoI GGC'GCC

Cuts at:      0     849     927    1394    3303 
   Size:        849      78     467    1909

  Fragments arranged by size: 

                1909     849     467      78
 
SmaI CCC'GGG

Cuts at:      0    1519    2078    3303 
   Size:       1519     559    1225

  Fragments arranged by size: 

                1519    1225     559
 
SmlI C'TyrA_G

Cuts at:      0     304     598    3303 
   Size:        304     294    2705

  Fragments arranged by size: 

                2705     304     294
 
SphI G_CATG'C

Cuts at:      0    1512    1536    2571    3303 
   Size:       1512      24    1035     732

  Fragments arranged by size: 

                1512    1035     732      24
 
StyI C'CwwG_G

Cuts at:      0       8     440     656     830    1490    1583    1745    2465 
   Size:          8     432     216     174     660      93     162     720

Cuts at:   2465    2813    2867    3303 
   Size:        348      54     436

  Fragments arranged by size: 

                 720     660     436     432     348     216     174     162
                  93      54       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     415    1010    1299    1883    3303 
   Size:        415     595     289     584    1420

  Fragments arranged by size: 

                1420     595     584     415     289
 
TatI w'GTAC_w

Cuts at:      0     584     860     917    2651    3303 
   Size:        584     276      57    1734     652

  Fragments arranged by size: 

                1734     652     584     276      57
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2339    2371    3303 
   Size:       2339      32     932

  Fragments arranged by size: 

                2339     932      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     391    1549    2758    3303 
   Size:        391    1158    1209     545

  Fragments arranged by size: 

                1209    1158     545     391
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1245    3303 
   Size:       1245    2058
 
XhoI C'TCGA_G

Cuts at:      0     598    3303 
   Size:        598    2705
 
XmaI C'CCGG_G

Cuts at:      0    1517    2076    3303 
   Size:       1517     559    1227

  Fragments arranged by size: 

                1517    1227     559
 
XmnI GAAnn'nnTTC

Cuts at:      0      82    3303 
   Size:         82    3221
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NspI     PasI    PflMI     PfoI
    PpuMI   PspOMI     PstI    PvuII     SacI     ScaI    SexAI     SfcI
     SfoI     SmaI     SmlI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI
    BfrBI     BmtI     BplI    BsaAI    BsiEI    BsiWI     BsmI   BssHII
    BstBI     ClaI     DraI     DrdI     EagI    EcoRI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     MfeI     MluI     NaeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     XbaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com