Restriction map for pF1KSDB0034
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# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       ApoI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BalI        BbrPI       
BbvCI       BciVI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BsaWI       BseAI       BsiEI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrGI       BstBI       BstEII      
BstPI       CelII       ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NcoI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SapI        ScaI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        Tru9I       XbaI        
XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccI         1206  GTATAC               GT^[AC][GT]AC
AflIII       1475  ACACGT               A^C[GA][CT]GT
AscI         1721  GGCGCGCC             GG^CGCGCC
AspI         1813  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         674  GGATCC               G^GATCC
BbeI         1621  GGCGCC               GGCGC^C
BfaI         1781  CTAG                 C^TAG
BglII         680  AGATCT               A^GATCT
BsaAI        1476  CACGTA               [CT]AC^GT[GA]
BsiWI        1478  CGTACG               C^GTACG
BspHI        1505  TCATGA               T^CATGA
Bst1107I     1206  GTATAC               GTA^TAC
BstZ17I      1206  GTATAC               GTA^TAC
DraIII        700  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1570  GACAGCATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KasI         1621  GGCGCC               G^GCGCC
MaeI         1781  CTAG                 C^TAG
NarI         1621  GGCGCC               GG^CGCC
NheI         1780  GCTAGC               G^CTAGC
PflFI        1813  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII          40  CAGCTG               CAG^CTG
RcaI         1505  TCATGA               T^CATGA
SbfI         1518  CCTGCAGG             CCTGCA^GG
SexAI         587  ACCTGGT              A^CC[AT]GGT
SfiI          768  GGCCATGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1621  GGCGCC               GGC^GCC
Sse8387I     1518  CCTGCAGG             CCTGCA^GG
TfiI          461  GAATC                G^A[AT]TC
Tsp509I       804  AATT                 ^AATT
Tth111I      1813  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1454  CCAGGTTCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcyI          688  GACGCC               G[GA]^CG[CT]C
             1621  GGCGCC              
AocI          466  CCTGAGG              CC^T[ACGT]AGG
             1921  CCTAAGG             
Asp700I        90  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1335  GAAGAGGTTC          
AvaI           84  CTCGGG               C^[CT]CG[GA]G
             1933  CCCGAG              
BbsI           79  GTCTTC               GAAGAC(2/6)
              961  GTCTTC              
BcgI         1443  CGAGGACTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2079  CGAGGACAGTGC        
BglI          769  GCCATGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1930  GCCCCCGAGGC         
BpuAI          79  GTCTTC               GAAGAC(2/6)
              961  GTCTTC              
BsaBI         675  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1365  GATCAGCATC          
BsaHI         688  GACGCC               G[GA]^CG[CT]C
             1621  GGCGCC              
BsaI         1216  GAGACC               GGTCTC(1/5)
             1737  GGTCTC              
BsoBI          84  CTCGGG               C^[CT]CG[GA]G
             1933  CCCGAG              
BsrFI         765  GCCGGC               [GA]^CCGG[CT]
             1876  GCCGGC              
BssHII        724  GCGCGC               G^CGCGC
             1722  GCGCGC              
BssSI         209  CACGAG               CACGAG(-5/-1)
              142  CTCGTG              
BstXI        1727  CCAAGCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2019  CCAGGAGACTGG        
Bsu36I        466  CCTGAGG              CC^T[ACGT]AGG
             1921  CCTAAGG             
Cfr10I        765  GCCGGC               [GA]^CCGG[CT]
             1876  GCCGGC              
EaeI          767  CGGCCA               [CT]^GGCC[GA]
             1613  TGGCCG              
EarI           94  CTCTTC               CTCTTC(1/4)
             1335  GAAGAG              
Eco81I        466  CCTGAGG              CC^T[ACGT]AGG
             1921  CCTAAGG             
EcoNI         852  CCTCCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1224  CCTGCACCAGG         
HaeII        1353  GGCGCT               [GA]GCGC^[CT]
             1621  GGCGCC              
Hin1I         688  GACGCC               G[GA]^CG[CT]C
             1621  GGCGCC              
Ksp632I        94  CTCTTC               CTCTTC(1/4)
             1335  GAAGAG              
KspI          634  CCGCGG               CCGC^GG
             1538  CCGCGG              
MamI          675  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1365  GATCAGCATC          
NaeI          765  GCCGGC               GCC^GGC
             1876  GCCGGC              
NgoMIV        765  GCCGGC               G^CCGGC
             1876  GCCGGC              
NspI          512  GCATGT               [GA]CATG^[CT]
             1178  ACATGC              
PpuMI         200  AGGTCCC              [GA]G^G[AT]CC[CT]
             1283  GGGTCCT             
SacI          268  GAGCTC               GAGCT^C
              625  GAGCTC              
SacII         634  CCGCGG               CCGC^GG
             1538  CCGCGG              
SmlI          325  CTCAAG               C^T[CT][GA]AG
             1384  CTCAAG              
StuI         1833  AGGCCT               AGG^CCT
             1937  AGGCCT              
XmnI           90  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1335  GAAGAGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrBI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XhoII       
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