Restriction map for pF1KSDB0030
 (Linear) (Six-base) MAPSORT of: KIBB0030.seq  Check: 9029  from: 1  to: 4818



KIBB0030.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3567    4818 
   Size:       3567    1251
 
AatII G_ACGT'C

Cuts at:      0    2935    4818 
   Size:       2935    1883
 
AccI GT'mk_AC

Cuts at:      0     260    3874    3952    4291    4818 
   Size:        260    3614      78     339     527

  Fragments arranged by size: 

                3614     527     339     260      78
 
Acc65I G'GTAC_C

Cuts at:      0    3408    4818 
   Size:       3408    1410
 
AclI AA'CG_TT

Cuts at:      0    1851    4818 
   Size:       1851    2967
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     767    1142    1343    2680    2764    3561    3739    4265 
   Size:        767     375     201    1337      84     797     178     526

Cuts at:   4265    4393    4818 
   Size:        128     425

  Fragments arranged by size: 

                1337     797     767     526     425     375     201     178
                 128      84
 
AflII C'TTAA_G

Cuts at:      0    1263    2791    4818 
   Size:       1263    1528    2027

  Fragments arranged by size: 

                2027    1528    1263
 
AflIII A'CryG_T

Cuts at:      0    2471    3435    4025    4448    4818 
   Size:       2471     964     590     423     370

  Fragments arranged by size: 

                2471     964     590     423     370
 
AgeI A'CCGG_T

Cuts at:      0     722    1020    4818 
   Size:        722     298    3798

  Fragments arranged by size: 

                3798     722     298
 
AleI CACnn'nnGTG

Cuts at:      0     490    1537    4818 
   Size:        490    1047    3281

  Fragments arranged by size: 

                3281    1047     490
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1723    3007    3828    4818 
   Size:       1723    1284     821     990

  Fragments arranged by size: 

                1723    1284     990     821
 
AlwNI CAG_nnn'CTG

Cuts at:      0     232    1033    1549    1693    2355    2527    3355    3825 
   Size:        232     801     516     144     662     172     828     470

Cuts at:   3825    3921    4818 
   Size:         96     897

  Fragments arranged by size: 

                 897     828     801     662     516     470     232     172
                 144      96
 
ApaI G_GGCC'C

Cuts at:      0     189     621    2069    4818 
   Size:        189     432    1448    2749

  Fragments arranged by size: 

                2749    1448     432     189
 
ApoI r'AATT_y

Cuts at:      0     430     563    1091    1512    1760    4234    4818 
   Size:        430     133     528     421     248    2474     584

  Fragments arranged by size: 

                2474     584     528     430     421     248     133
 
AvaI C'yCGr_G

Cuts at:      0      72     195     212    1786    2681    4818 
   Size:         72     123      17    1574     895    2137

  Fragments arranged by size: 

                2137    1574     895     123      72      17
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     361     394    4818 
   Size:        361      33    4424

  Fragments arranged by size: 

                4424     361      33
 
BamHI G'GATC_C

Cuts at:      0     271    1977    4818 
   Size:        271    1706    2841

  Fragments arranged by size: 

                2841    1706     271
 
BanI G'GyrC_C

Cuts at:      0     448    1317    2991    3408    4077    4818 
   Size:        448     869    1674     417     669     741

  Fragments arranged by size: 

                1674     869     741     669     448     417
 
BanII G_rGCy'C

Cuts at:      0     189     399     621    1242    2069    2447    3715    4464 
   Size:        189     210     222     621     827     378    1268     749

Cuts at:   4464    4818 
   Size:        354

  Fragments arranged by size: 

                1268     827     749     621     378     354     222     210
                 189
 
BbeI G_GCGC'C

Cuts at:      0     452    1321    4818 
   Size:        452     869    3497

  Fragments arranged by size: 

                3497     869     452
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1760    2935    4818 
   Size:       1760    1175    1883

  Fragments arranged by size: 

                1883    1760    1175
 
BbvCI CC'TCA_GC

Cuts at:      0    3998    4818 
   Size:       3998     820
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4533    4567    4818 
   Size:       4533      34     251

  Fragments arranged by size: 

                4533     251      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    4452    4818 
   Size:       4452     366
 
BfrBI ATG'CAT

Cuts at:      0    2769    4818 
   Size:       2769    2049
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2103    3627    4606    4818 
   Size:       2103    1524     979     212

  Fragments arranged by size: 

                2103    1524     979     212
 
BglII A'GATC_T

Cuts at:      0     299    1125    4818 
   Size:        299     826    3693

  Fragments arranged by size: 

                3693     826     299
 
BlpI GC'TnA_GC

Cuts at:      0    4222    4730    4818 
   Size:       4222     508      88

  Fragments arranged by size: 

                4222     508      88
 
Bme1580I G_kGCm'C

Cuts at:      0     189     621    1199    1712    2069    2504    4082    4332 
   Size:        189     432     578     513     357     435    1578     250

Cuts at:   4332    4818 
   Size:        486

  Fragments arranged by size: 

                1578     578     513     486     435     432     357     250
                 189
 
BmgBI CAC'GTC

Cuts at:      0    1717    1935    2964    3436    4680    4818 
   Size:       1717     218    1029     472    1244     138

  Fragments arranged by size: 

                1717    1244    1029     472     218     138
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     499     823     950    1264    3700    4818 
   Size:        499     324     127     314    2436    1118

  Fragments arranged by size: 

                2436    1118     499     324     314     127
 
BmtI G_CTAG'C

Cuts at:      0    3647    4818 
   Size:       3647    1171
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3846    4818 
   Size:       3846     972
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685     947    1039    1328    3110    3302    3503    3901 
   Size:        685     262      92     289    1782     192     201     398

Cuts at:   3901    4171    4381    4588    4818 
   Size:        270     210     207     230

  Fragments arranged by size: 

                1782     685     398     289     270     262     230     210
                 207     201     192      92
 
Bpu10I CC'TnA_GC

Cuts at:      0    1376    2053    3321    3998    4818 
   Size:       1376     677    1268     677     820

  Fragments arranged by size: 

                1376    1268     820     677     677
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     656    1520    2836    3477    4818 
   Size:        656     864    1316     641    1341

  Fragments arranged by size: 

                1341    1316     864     656     641
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     164    1598    1729    3479    3488    4113    4818 
   Size:        164    1434     131    1750       9     625     705

  Fragments arranged by size: 

                1750    1434     705     625     164     131       9
 
BsaAI yAC'GTr

Cuts at:      0    4026    4818 
   Size:       4026     792
 
BsaBI GATnn'nnATC

Cuts at:      0    2255    4818 
   Size:       2255    2563
 
BsaHI Gr'CG_yC

Cuts at:      0     449     688    1318    2655    2932    4818 
   Size:        449     239     630    1337     277    1886

  Fragments arranged by size: 

                1886    1337     630     449     277     239
 
BsaWI w'CCGG_w

Cuts at:      0     722    1020    4818 
   Size:        722     298    3798

  Fragments arranged by size: 

                3798     722     298
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     298     328     595     625    4818 
   Size:        298      30     267      30    4193

  Fragments arranged by size: 

                4193     298     267      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     616     965    1154    1300    1396    1472    1575    1855 
   Size:        616     349     189     146      96      76     103     280

Cuts at:   1855    3462    3506    3607    3751    3754    4318    4357    4813 
   Size:       1607      44     101     144       3     564      39     456

Cuts at:   4813    4816    4818 
   Size:          3       2

  Fragments arranged by size: 

                1607     616     564     456     349     280     189     146
                 144     103     101      96      76      44      39       3
                   3       2
 
BseYI C'CCAG_C

Cuts at:      0     188     246     309     791    1170    1371    2227    3296 
   Size:        188      58      63     482     379     201     856    1069

Cuts at:   3296    3363    3617    3940    4818 
   Size:         67     254     323     878

  Fragments arranged by size: 

                1069     878     856     482     379     323     254     201
                 188      67      63      58
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     238     245     406     493    3100    3463    3838 
   Size:        224      14       7     161      87    2607     363     375

Cuts at:   3838    3907    4818 
   Size:         69     911

  Fragments arranged by size: 

                2607     911     375     363     224     161      87      69
                  14       7
 
BsiEI CG_ry'CG

Cuts at:      0    1860    4818 
   Size:       1860    2958
 
BsiHKAI G_wGCw'C

Cuts at:      0    3071    4818 
   Size:       3071    1747
 
BsmI GAATG_Cn'

Cuts at:      0    4264    4818 
   Size:       4264     554
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     679    1557    4818 
   Size:        679     878    3261

  Fragments arranged by size: 

                3261     878     679
 
Bsp1286I G_dGCh'C

Cuts at:      0     189     399     621    1199    1242    1712    2069    2447 
   Size:        189     210     222     578      43     470     357     378

Cuts at:   2447    2504    3071    3715    4082    4332    4464    4818 
   Size:         57     567     644     367     250     132     354

  Fragments arranged by size: 

                 644     578     567     470     378     367     357     354
                 250     222     210     189     132      57      43
 
BspHI T'CATG_A

Cuts at:      0    1095    4466    4818 
   Size:       1095    3371     352

  Fragments arranged by size: 

                3371    1095     352
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3567    4649    4818 
   Size:       3567    1082     169

  Fragments arranged by size: 

                3567    1082     169
 
BsrBI CCG'CTC

Cuts at:      0    3507    3597    4818 
   Size:       3507      90    1221

  Fragments arranged by size: 

                3507    1221      90
 
BsrDI GCAATG_nn'

Cuts at:      0     740    1199    2165    3893    4818 
   Size:        740     459     966    1728     925

  Fragments arranged by size: 

                1728     966     925     740     459
 
BsrFI r'CCGG_y

Cuts at:      0      87     722     749    1020    2106    3536    4818 
   Size:         87     635      27     271    1086    1430    1282

  Fragments arranged by size: 

                1430    1282    1086     635     271      87      27
 
BssSI C'ACGA_G

Cuts at:      0    3899    4818 
   Size:       3899     919
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     232     742    2964    3921    3930    4818 
   Size:        232     510    2222     957       9     888

  Fragments arranged by size: 

                2222     957     888     510     232       9
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     492    1071    1539    4258    4818 
   Size:        492     579     468    2719     560

  Fragments arranged by size: 

                2719     579     560     492     468
 
BstYI r'GATC_y

Cuts at:      0     271     299     570    1125    1977    2970    3032    4154 
   Size:        271      28     271     555     852     993      62    1122

Cuts at:   4154    4818 
   Size:        664

  Fragments arranged by size: 

                1122     993     852     664     555     271     271      62
                  28
 
BstZ17I GTA'TAC

Cuts at:      0    4292    4818 
   Size:       4292     526
 
Bsu36I CC'TnA_GG

Cuts at:      0    1220    1721    3623    4818 
   Size:       1220     501    1902    1195

  Fragments arranged by size: 

                1902    1220    1195     501
 
BtgI C'CryG_G

Cuts at:      0    2061    2100    3144    3528    3666    4082    4818 
   Size:       2061      39    1044     384     138     416     736

  Fragments arranged by size: 

                2061    1044     736     416     384     138      39
 
BtsI GCAGTG_nn'

Cuts at:      0     207     623    1707    1963    3108    3894    4532    4818 
   Size:        207     416    1084     256    1145     786     638     286

  Fragments arranged by size: 

                1145    1084     786     638     416     286     256     207
            
 
ClaI AT'CG_AT

Cuts at:      0    2556    3254    4818 
   Size:       2556     698    1564

  Fragments arranged by size: 

                2556    1564     698
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     342     377    4818 
   Size:        342      35    4441

  Fragments arranged by size: 

                4441     342      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1086    4818 
   Size:       1086    3732
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2779    4818 
   Size:       2779    2039
 
EaeI y'GGCC_r

Cuts at:      0     712     751    1969    2040    2378    3092    4320    4818 
   Size:        712      39    1218      71     338     714    1228     498

  Fragments arranged by size: 

                1228    1218     714     712     498     338      71      39
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1400    2660    2669    2801    3850    4818 
   Size:       1400    1260       9     132    1049     968

  Fragments arranged by size: 

                1400    1260    1049     968     132       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685     767     947    1039    1142    1328    1343    2680 
   Size:        685      82     180      92     103     186      15    1337

Cuts at:   2680    2764    3110    3302    3503    3561    3739    3901    4171 
   Size:         84     346     192     201      58     178     162     270

Cuts at:   4171    4265    4381    4393    4588    4818 
   Size:         94     116      12     195     230

  Fragments arranged by size: 

                1337     685     346     270     230     201     195     192
                 186     180     178     162     116     103      94      92
                  84      82      58      15      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1667    3652    4300    4818 
   Size:       1667    1985     648     518

  Fragments arranged by size: 

                1985    1667     648     518
 
EcoO109I rG'GnC_Cy

Cuts at:      0      25     323     437     617    2066    2119    2140    2295 
   Size:         25     298     114     180    1449      53      21     155

Cuts at:   2295    3013    3130    4183    4608    4722    4818 
   Size:        718     117    1053     425     114      96

  Fragments arranged by size: 

                1449    1053     718     425     298     180     155     117
                 114     114      96      53      25      21
 
EcoRI G'AATT_C

Cuts at:      0     563    4818 
   Size:        563    4255
 
EcoRV GAT'ATC

Cuts at:      0     594    4818 
   Size:        594    4224
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1027    2830    3591    4237    4818 
   Size:       1027    1803     761     646     581

  Fragments arranged by size: 

                1803    1027     761     646     581
 
FspI TGC'GCA

Cuts at:      0    3634    4818 
   Size:       3634    1184
 
HaeII r_GCGC'y

Cuts at:      0      88     452    1321    2130    2494    2703    4594    4818 
   Size:         88     364     869     809     364     209    1891     224

  Fragments arranged by size: 

                1891     869     809     364     364     224     209      88
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     595     627     709     741    1129    1161    2794    2826 
   Size:        595      32      82      32     388      32    1633      32

Cuts at:   2826    3056    3088    3331    3363    3739    3771    4789    4818 
   Size:        230      32     243      32     376      32    1018      29

  Fragments arranged by size: 

                1633    1018     595     388     376     243     230      82
                  32      32      32      32      32      32      32      29
            
 
HincII GTy'rAC

Cuts at:      0    2753    4818 
   Size:       2753    2065
 
HpaI GTT'AAC

Cuts at:      0    2753    4818 
   Size:       2753    2065
 
KasI G'GCGC_C

Cuts at:      0     448    1317    4818 
   Size:        448     869    3501

  Fragments arranged by size: 

                3501     869     448
 
KpnI G_GTAC'C

Cuts at:      0    3412    4818 
   Size:       3412    1406
 
MfeI C'AATT_G

Cuts at:      0    2985    4818 
   Size:       2985    1833
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     835    1231    1581    4289    4469    4818 
   Size:        835     396     350    2708     180     349

  Fragments arranged by size: 

                2708     835     396     350     349     180
 
MscI TGG'CCA

Cuts at:      0    2380    3094    4322    4818 
   Size:       2380     714    1228     496

  Fragments arranged by size: 

                2380    1228     714     496
 
MslI CAynn'nnrTG

Cuts at:      0     490    1537    1928    2774    3076    3788    4818 
   Size:        490    1047     391     846     302     712    1030

  Fragments arranged by size: 

                1047    1030     846     712     490     391     302
 
MspA1I CmG'CkG

Cuts at:      0     250    1255    2355    2376    2480    3314    3918    3930 
   Size:        250    1005    1100      21     104     834     604      12

Cuts at:   3930    4359    4395    4729    4818 
   Size:        429      36     334      89

  Fragments arranged by size: 

                1100    1005     834     604     429     334     250     104
                  89      36      21      12
 
NaeI GCC'GGC

Cuts at:      0    2108    4818 
   Size:       2108    2710
 
NarI GG'CG_CC

Cuts at:      0     449    1318    4818 
   Size:        449     869    3500

  Fragments arranged by size: 

                3500     869     449
 
NcoI C'CATG_G

Cuts at:      0    4082    4818 
   Size:       4082     736
 
NdeI CA'TA_TG

Cuts at:      0    3908    4818 
   Size:       3908     910
 
NgoMIV G'CCGG_C

Cuts at:      0    2106    4818 
   Size:       2106    2712
 
NheI G'CTAG_C

Cuts at:      0    3643    4818 
   Size:       3643    1175
 
NsiI A_TGCA'T

Cuts at:      0    2771    4818 
   Size:       2771    2047
 
NspI r_CATG'y

Cuts at:      0    2475    4818 
   Size:       2475    2343
 
PasI CC'CwG_GG

Cuts at:      0     318    4296    4818 
   Size:        318    3978     522

  Fragments arranged by size: 

                3978     522     318
 
PciI A'CATG_T

Cuts at:      0    2471    4818 
   Size:       2471    2347
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2077    4818 
   Size:       2077    2741
 
PmlI CAC'GTG

Cuts at:      0    4026    4818 
   Size:       4026     792
 
PpuMI rG'GwC_Cy

Cuts at:      0     437    2119    2140    2295    3013    4183    4722    4818 
   Size:        437    1682      21     155     718    1170     539      96

  Fragments arranged by size: 

                1682    1170     718     539     437     155      96      21
            
 
PshAI GACnn'nnGTC

Cuts at:      0    3110    4474    4818 
   Size:       3110    1364     344

  Fragments arranged by size: 

                3110    1364     344
 
PspOMI G'GGCC_C

Cuts at:      0     185     617    2065    4818 
   Size:        185     432    1448    2753

  Fragments arranged by size: 

                2753    1448     432     185
 
PstI C_TGCA'G

Cuts at:      0     167    1671    2960    4067    4556    4818 
   Size:        167    1504    1289    1107     489     262

  Fragments arranged by size: 

                1504    1289    1107     489     262     167
 
PvuII CAG'CTG

Cuts at:      0     250    1255    2355    2376    3918    3930    4359    4818 
   Size:        250    1005    1100      21    1542      12     429     459

  Fragments arranged by size: 

                1542    1100    1005     459     429     250      21      12
            
 
SanDI GG'GwC_CC

Cuts at:      0    2119    2140    3013    4818 
   Size:       2119      21     873    1805

  Fragments arranged by size: 

                2119    1805     873      21
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2660    3850    4818 
   Size:       2660    1190     968

  Fragments arranged by size: 

                2660    1190     968
 
SbfI CC_TGCA'GG

Cuts at:      0    1671    4818 
   Size:       1671    3147
 
ScaI AGT'ACT

Cuts at:      0    3375    3964    4818 
   Size:       3375     589     854

  Fragments arranged by size: 

                3375     854     589
 
SfcI C'TryA_G

Cuts at:      0     163     425     908    1667    2956    4063    4552    4585 
   Size:        163     262     483     759    1289    1107     489      33

Cuts at:   4585    4701    4818 
   Size:        116     117

  Fragments arranged by size: 

                1289    1107     759     489     483     262     163     117
                 116      33
 
SfoI GGC'GCC

Cuts at:      0     450    1319    4818 
   Size:        450     869    3499

  Fragments arranged by size: 

                3499     869     450
 
SgrAI Cr'CCGG_yG

Cuts at:      0      87    4818 
   Size:         87    4731
 
SmaI CCC'GGG

Cuts at:      0      74    1788    4818 
   Size:         74    1714    3030

  Fragments arranged by size: 

                3030    1714      74
 
SmlI C'TyrA_G

Cuts at:      0     671    1263    1535    2791    2815    3456    4818 
   Size:        671     592     272    1256      24     641    1362

  Fragments arranged by size: 

                1362    1256     671     641     592     272      24
 
SpeI A'CTAG_T

Cuts at:      0    3370    4818 
   Size:       3370    1448
 
StyI C'CwwG_G

Cuts at:      0     465     579    1295    2037    2071    3095    3758    3969 
   Size:        465     114     716     742      34    1024     663     211

Cuts at:   3969    4082    4818 
   Size:        113     736

  Fragments arranged by size: 

                1024     742     736     716     663     465     211     114
                 113      34
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     651    2200    2979    3503    4071    4818 
   Size:        651    1549     779     524     568     747

  Fragments arranged by size: 

                1549     779     747     651     568     524
 
TatI w'GTAC_w

Cuts at:      0    3373    3962    4818 
   Size:       3373     589     856

  Fragments arranged by size: 

                3373     856     589
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2454    2880    4818 
   Size:       2454     426    1938

  Fragments arranged by size: 

                2454    1938     426
 
XmaI C'CCGG_G

Cuts at:      0      72    1786    4818 
   Size:         72    1714    3032

  Fragments arranged by size: 

                3032    1714      72
 
XmnI GAAnn'nnTTC

Cuts at:      0    3385    4818 
   Size:       3385    1433
 
ZraI GAC'GTC

Cuts at:      0    2933    4818 
   Size:       2933    1885
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AleI     AlfI    AlwNI     ApaI     ApoI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NsiI     NspI     PasI
     PciI    PflMI     PmlI    PpuMI    PshAI   PspOMI     PstI    PvuII
    SanDI     SapI     SbfI     ScaI     SfcI     SfoI    SgrAI     SmaI
     SmlI     SpeI     StyI    TaqII     TatI     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI     AhdI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BclI    BsiWI    BspEI    BsrGI   BssHII    BstBI   BstEII    BtgZI
     DraI     EagI     EciI  EcoICRI     FseI    FspAI  HindIII     MluI
     NotI     NruI     PacI     PfoI     PmeI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SexAI     SfiI
     SgfI    SnaBI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI
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