Restriction map for pF1KSDB0017
##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AscI        
Asp718I     AvrII       BamHI       BclI        BfrI        
BglII       BlnI        BsaBI       BsiWI       BsmI        
BspDI       BspHI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        DraI        EcoO65I     EcoT22I     FbaI        
HpaI        KpnI        MamI        MfeI        MunI        
NdeI        NheI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        Psp1406I    PvuI        
RcaI        SalI        SfiI        SfuI        SgrAI       
SmlI        SnaBI       SphI        SspI        SwaI        
XbaI        XhoI        


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# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII        2844  GACGTC               GACGT^C
Alw44I       5101  GTGCAC               G^TGCAC
AocI         4033  CCTGAGG              CC^T[ACGT]AGG
ApaLI        5101  GTGCAC               G^TGCAC
AseI         2316  ATTAAT               AT^TAAT
AsnI         2316  ATTAAT               AT^TAAT
AviII        1382  TGCGCA               TGC^GCA
BbrPI        4807  CACGTG               CAC^GTG
BbvCI        3374  CCTCAGC              CCTCAGC(-5/-2)
BcgI         3866  GCATCCCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2837  GGATAC               GTATCC(6/5)
BsaAI        4807  CACGTG               [CT]AC^GT[GA]
BsmBI        3885  CGTCTC               CGTCTC(1/5)
BspLU11I      824  ACATGT               A^CATGT
BstXI        4679  CCAAGGACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       4033  CCTGAGG              CC^T[ACGT]AGG
CpoI          622  CGGACCG              CG^G[AT]CCG
EagI          277  CGGCCG               C^GGCCG
EclXI         277  CGGCCG               C^GGCCG
Eco52I        277  CGGCCG               C^GGCCG
Eco81I       4033  CCTGAGG              CC^T[ACGT]AGG
EcoRI         523  GAATTC               G^AATTC
EcoRV        4999  GATATC               GAT^ATC
FseI         3901  GGCCGGCC             GGCCGG^CC
FspI         1382  TGCGCA               TGC^GCA
HincII       1161  GTTGAC               GT[CT]^[GA]AC
HindII       1161  GTTGAC               GT[CT]^[GA]AC
MluI         1750  ACGCGT               A^CGCGT
NotI          276  GCGGCCGC             GC^GGCCGC
NruI         1817  TCGCGA               TCG^CGA
PmaCI        4807  CACGTG               CAC^GTG
PmlI         4807  CACGTG               CAC^GTG
PshBI        2316  ATTAAT               AT^TAAT
RsrII         622  CGGACCG              CG^G[AT]CCG
ScaI         2809  AGTACT               AGT^ACT
SpeI         2812  ACTAGT               A^CTAGT


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI         2365  GTCTAC               GT^[AC][GT]AC
             4744  GTCTAC              
AhdI         1399  GACGTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4729  GACTTTGTGTC         
Aor51HI      3584  AGCGCT               AGC^GCT
             3765  AGCGCT              
ApaI         2801  GGGCCC               GGGCC^C
             3236  GGGCCC              
AspEI        1399  GACGTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4729  GACTTTGTGTC         
Bsp1407I     1724  TGTACA               T^GTACA
             3614  TGTACA              
BsrGI        1724  TGTACA               T^GTACA
             3614  TGTACA              
BstAPI        294  GCACGCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3972  GCAGACTCTGC         
DraIII        226  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              295  CACGCGGTG           
Eam1105I     1399  GACGTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4729  GACTTTGTGTC         
Eco47III     3584  AGCGCT               AGC^GCT
             3765  AGCGCT              
HindIII      3061  AAGCTT               A^AGCTT
             4771  AAGCTT              
KspI          122  CCGCGG               CCGC^GG
             2582  CCGCGG              
NcoI          977  CCATGG               C^CATGG
             3270  CCATGG              
PshAI        1143  GACCATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1683  GACAGCCGTC          
SacII         122  CCGCGG               CCGC^GG
             2582  CCGCGG              
SexAI        1505  ACCAGGT              A^CC[AT]GGT
             4841  ACCTGGT             
SspBI        1724  TGTACA               T^GTACA
             3614  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  147 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApoI        Asp700I     AspHI       AspI        AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbsI        BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspEI       BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MroI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflFI       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      SbfI        ScrFI       
SfaNI       SfcI        SfoI        SmaI        Sse8387I    
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI        XmnI        
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