Restriction map for pF1KSDB0002
##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       AccI        AclI        AflII       AgeI        
AhdI        Aor51HI     AseI        AsnI        Asp700I     
AspEI       AvrII       BbeI        BbrPI       BfrI        
BlnI        BsiWI       BsmBI       Bsp1407I    BspDI       
BspHI       BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
DrdI        Eam1105I    Eco47III    EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        HincII      HindII      
HindIII     HpaI        KasI        MfeI        MluI        
MunI        NarI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SwaI        
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        317  GGTACC               G^GTACC
AcsI         2698  GAATTT               [GA]^AATT[CT]
AcyI         1952  GACGCC               G[GA]^CG[CT]C
AflIII       2201  ACATGT               A^C[GA][CT]GT
Alw44I       3582  GTGCAC               G^TGCAC
ApaLI        3582  GTGCAC               G^TGCAC
ApoI         2698  GAATTT               [GA]^AATT[CT]
AscI          123  GGCGCGCC             GG^CGCGCC
Asp718I       317  GGTACC               G^GTACC
AspI         1614  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        2592  GGATCC               G^GATCC
BbsI         1611  GAAGAC               GAAGAC(2/6)
BciVI        2455  GGATAC               GTATCC(6/5)
BclI         2709  TGATCA               T^GATCA
BglII        1545  AGATCT               A^GATCT
BlpI         1383  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1383  GCTAAGC              GC^T[ACGT]AGC
BpuAI        1611  GAAGAC               GAAGAC(2/6)
BsaAI        3001  TACGTG               [CT]AC^GT[GA]
BsaHI        1952  GACGCC               G[GA]^CG[CT]C
BsaI          751  GAGACC               GGTCTC(1/5)
BsiEI         605  CGGCCG               CG[GA][CT]^CG
BsmI          475  GCATTC               GAATGC(1/-1)
BspLU11I     2201  ACATGT               A^CATGT
CelII        1383  GCTAAGC              GC^T[ACGT]AGC
EagI          605  CGGCCG               C^GGCCG
EclXI         605  CGGCCG               C^GGCCG
Eco52I        605  CGGCCG               C^GGCCG
FbaI         2709  TGATCA               T^GATCA
Hin1I        1952  GACGCC               G[GA]^CG[CT]C
KpnI          317  GGTACC               GGTAC^C
KspI         1368  CCGCGG               CCGC^GG
NheI         2917  GCTAGC               G^CTAGC
PaeR7I        130  CTCGAG               C^TCGAG
PflFI        1614  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacII        1368  CCGCGG               CCGC^GG
ScaI          134  AGTACT               AGT^ACT
SexAI         245  ACCTGGT              A^CC[AT]GGT
SfiI          765  GGCCATGCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI           71  GCATGC               GCATG^C
SspI         2692  AATATT               AAT^ATT
Tsp509I      2699  AATT                 ^AATT
Tth111I      1614  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          130  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AocI         2257  CCTGAGG              CC^T[ACGT]AGG
             3574  CCTCAGG             
AviII          38  TGCGCA               TGC^GCA
               56  TGCGCA              
BbvCI        3110  CCTCAGC              CCTCAGC(-5/-2)
             3450  GCTGAGG             
BsaBI         537  GATTGGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              624  GATGAACATC          
BspMI          53  ACCTGC               ACCTGC(4/8)
              714  GCAGGT              
BssHII         96  GCGCGC               G^CGCGC
              124  GCGCGC              
Bsu36I       2257  CCTGAGG              CC^T[ACGT]AGG
             3574  CCTCAGG             
DraIII        244  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3320  CACCCAGTG           
Eco81I       2257  CCTGAGG              CC^T[ACGT]AGG
             3574  CCTCAGG             
EcoNI         423  CCTTGGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2179  CCTTGCACAGG         
FspI           38  TGCGCA               TGC^GCA
               56  TGCGCA              
MamI          537  GATTGGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              624  GATGAACATC          
NcoI         1197  CCATGG               C^CATGG
             3089  CCATGG              
PflMI        1678  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1962  CCAGAGAGTGG         
StuI         2929  AGGCCT               AGG^CCT
             3454  AGGCCT              
TfiI         1342  GATTC                G^A[AT]TC
             2416  GATTC               
Van91I       1678  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1962  CCAGAGAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccII       AccIII      AciI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BcgI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspEI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StyI        
TaqI        Tru9I       TseI        Tsp45I      TspRI       
TthHB8I     XcmI        XhoII       XmaCI       XmaI        
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