Restriction map for pF1KSDA1970
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AvaI        
AviII       AvrII       BbvCI       BcgI        BciVI       
BfrI        BlnI        BlpI        Bpu1102I    BsaBI       
BseAI       BsiEI       BsiWI       BsmI        BsoBI       
BspDI       BspEI       BspLU11I    BsrBI       BsrFI       
BssHII      BssSI       BstBI       BstXI       CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        EclXI       Eco52I      
EcoRV       EcoT22I     FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        SwaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AcsI         1057  GAATTC               [GA]^AATT[CT]
AhdI         1243  GACTTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       387  AGCGCT               AGC^GCT
ApaI          337  GGGCCC               GGGCC^C
ApoI         1057  GAATTC               [GA]^AATT[CT]
AspEI        1243  GACTTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         1106  GGCGCC               GGCGC^C
BbrPI         727  CACGTG               CAC^GTG
BbsI          396  GAAGAC               GAAGAC(2/6)
BclI          989  TGATCA               T^GATCA
BglI          131  GCCCCACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1363  AGATCT               A^GATCT
BpuAI         396  GAAGAC               GAAGAC(2/6)
BsaI          644  GAGACC               GGTCTC(1/5)
BsaWI         400  ACCGGA               [AT]^CCGG[AT]
BsmBI         753  CGTCTC               CGTCTC(1/5)
Bsp1407I      173  TGTACA               T^GTACA
BspHI         656  TCATGA               T^CATGA
BsrDI        1091  GCAATG               GCAATG(2/0)
BsrGI         173  TGTACA               T^GTACA
Bst1107I     1258  GTATAC               GTA^TAC
BstAPI        576  GCAGGTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1014  GGTCACC              G^GT[ACGT]ACC
BstPI        1014  GGTCACC              G^GT[ACGT]ACC
BstZ17I      1258  GTATAC               GTA^TAC
Eam1105I     1243  GACTTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          660  GAAGAG               CTCTTC(1/4)
Eco47III      387  AGCGCT               AGC^GCT
EcoO65I      1014  GGTCACC              G^GT[ACGT]ACC
EcoRI        1057  GAATTC               G^AATTC
FbaI          989  TGATCA               T^GATCA
KasI         1106  GGCGCC               G^GCGCC
Ksp632I       660  GAAGAG               CTCTTC(1/4)
MseI         1378  TTAA                 T^TAA
MslI          148  CACCTGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         1106  GGCGCC               GG^CGCC
NspI          278  ACATGC               [GA]CATG^[CT]
PmaCI         727  CACGTG               CAC^GTG
PmlI          727  CACGTG               CAC^GTG
PshAI        1008  GACACAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          656  TCATGA               T^CATGA
SacI          442  GAGCTC               GAGCT^C
SapI          660  GAAGAGC              GCTCTTC(1/4)
SbfI         1236  CCTGCAGG             CCTGCA^GG
SexAI         599  ACCTGGT              A^CC[AT]GGT
SfoI         1106  GGCGCC               GGC^GCC
Sse8387I     1236  CCTGCAGG             CCTGCA^GG
SspBI         173  TGTACA               T^GTACA
SspI          268  AATATT               AAT^ATT
TaqI         1293  TCGA                 T^CGA
TfiI          913  GATTC                G^A[AT]TC
Tru9I        1378  TTAA                 T^TAA
Tsp509I      1058  AATT                 ^AATT
TthHB8I      1293  TCGA                 T^CGA
XbaI         1356  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AccI         1189  GTCTAC               GT^[AC][GT]AC
             1258  GTATAC              
AocI           12  CCTGAGG              CC^T[ACGT]AGG
              216  CCTGAGG             
BalI          536  TGGCCA               TGG^CCA
              629  TGGCCA              
BamHI        1202  GGATCC               G^GATCC
             1547  GGATCC              
BsaAI         727  CACGTG               [CT]AC^GT[GA]
             1192  TACGTG              
BsgI         1069  CTGCAC               GTGCAG(16/14)
              498  GTGCAG              
Bsu36I         12  CCTGAGG              CC^T[ACGT]AGG
              216  CCTGAGG             
Eco81I         12  CCTGAGG              CC^T[ACGT]AGG
              216  CCTGAGG             
EcoT14I       539  CCAAGG               C^C[AT][AT]GG
             1520  CCTTGG              
FauI          300  CCCGC                CCCGC(4/6)
              317  CCCGC               
HgaI          477  GACGC                GACGC(5/10)
             1306  GACGC               
MluNI         536  TGGCCA               TGG^CCA
              629  TGGCCA              
MscI          536  TGGCCA               TGG^CCA
              629  TGGCCA              
PleI          240  GACTC                GAGTC(4/5)
             1275  GACTC               
StyI          539  CCAAGG               C^C[AT][AT]GG
             1520  CCTTGG              
XcmI          677  CCACATACCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1337  CCAAGCGTGTGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AspI        AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoNI       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflFI       PflMI       PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        TseI        Tsp45I      
TspRI       Tth111I     Van91I      XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com