Restriction map for
pF1KSDA1970
(Linear) (Six-base) MAPSORT of: KIAA1970.seq Check: 4160 from: 1 to: 1569
KIAA1970.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 510 567 779 827 1569
Size: 510 57 212 48 742
Fragments arranged by size:
742 510 212 57 48
AccI GT'mk_AC
Cuts at: 0 1190 1259 1569
Size: 1190 69 310
Fragments arranged by size:
1190 310 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 469 1426 1569
Size: 415 54 957 143
Fragments arranged by size:
957 415 143 54
AfeI AGC'GCT
Cuts at: 0 389 1569
Size: 389 1180
AhdI GACnn_n'nnGTC
Cuts at: 0 1248 1569
Size: 1248 321
AleI CACnn'nnGTG
Cuts at: 0 152 1569
Size: 152 1417
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 908 994 1569
Size: 908 86 575
Fragments arranged by size:
908 575 86
AlwNI CAG_nnn'CTG
Cuts at: 0 438 534 600 789 1083 1569
Size: 438 96 66 189 294 486
Fragments arranged by size:
486 438 294 189 96 66
ApaI G_GGCC'C
Cuts at: 0 341 1569
Size: 341 1228
ApoI r'AATT_y
Cuts at: 0 1057 1569
Size: 1057 512
BamHI G'GATC_C
Cuts at: 0 1202 1547 1569
Size: 1202 345 22
Fragments arranged by size:
1202 345 22
BanI G'GyrC_C
Cuts at: 0 582 1106 1432 1569
Size: 582 524 326 137
Fragments arranged by size:
582 524 326 137
BanII G_rGCy'C
Cuts at: 0 316 341 446 1569
Size: 316 25 105 1123
Fragments arranged by size:
1123 316 105 25
BbeI G_GCGC'C
Cuts at: 0 1110 1569
Size: 1110 459
BbsI GAAGACnn'nnnn_
Cuts at: 0 403 1569
Size: 403 1166
BclI T'GATC_A
Cuts at: 0 989 1569
Size: 989 580
BglI GCCn_nnn'nGGC
Cuts at: 0 137 1569
Size: 137 1432
BglII A'GATC_T
Cuts at: 0 1363 1569
Size: 1363 206
Bme1580I G_kGCm'C
Cuts at: 0 91 341 1569
Size: 91 250 1228
Fragments arranged by size:
1228 250 91
BmrI ACTGGGnnnn_n'
Cuts at: 0 643 1180 1249 1569
Size: 643 537 69 320
Fragments arranged by size:
643 537 320 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1397 1569
Size: 1397 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 460 592 901 1060 1063 1569
Size: 304 156 132 309 159 3 506
Fragments arranged by size:
506 309 304 159 156 132 3
Bpu10I CC'TnA_GC
Cuts at: 0 945 1569
Size: 945 624
BsaI GGTCTCn'nnnn_
Cuts at: 0 638 1569
Size: 638 931
BsaAI yAC'GTr
Cuts at: 0 729 1194 1569
Size: 729 465 375
Fragments arranged by size:
729 465 375
BsaHI Gr'CG_yC
Cuts at: 0 478 1107 1307 1569
Size: 478 629 200 262
Fragments arranged by size:
629 478 262 200
BsaWI w'CCGG_w
Cuts at: 0 400 1569
Size: 400 1169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 635 665 1058 1088 1569
Size: 635 30 393 30 481
Fragments arranged by size:
635 481 393 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 766 820 1156 1569
Size: 30 736 54 336 413
Fragments arranged by size:
736 413 336 54 30
BseYI C'CCAG_C
Cuts at: 0 127 334 431 1347 1569
Size: 127 207 97 916 222
Fragments arranged by size:
916 222 207 127 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 1054 1569
Size: 519 535 515
Fragments arranged by size:
535 519 515
BsiHKAI G_wGCw'C
Cuts at: 0 446 887 1299 1569
Size: 446 441 412 270
Fragments arranged by size:
446 441 412 270
BsmBI CGTCTCn'nnnn_
Cuts at: 0 759 1569
Size: 759 810
Bsp1286I G_dGCh'C
Cuts at: 0 91 316 341 446 887 1299 1569
Size: 91 225 25 105 441 412 270
Fragments arranged by size:
441 412 270 225 105 91 25
BspHI T'CATG_A
Cuts at: 0 656 1569
Size: 656 913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 510 567 779 827 1119 1569
Size: 510 57 212 48 292 450
Fragments arranged by size:
510 450 292 212 57 48
BsrDI GCAATG_nn'
Cuts at: 0 1098 1569
Size: 1098 471
BsrGI T'GTAC_A
Cuts at: 0 173 1569
Size: 173 1396
BstAPI GCAn_nnn'nTGC
Cuts at: 0 582 1569
Size: 582 987
BstEII G'GTnAC_C
Cuts at: 0 1014 1569
Size: 1014 555
BstYI r'GATC_y
Cuts at: 0 1202 1363 1547 1569
Size: 1202 161 184 22
Fragments arranged by size:
1202 184 161 22
BstZ17I GTA'TAC
Cuts at: 0 1260 1569
Size: 1260 309
Bsu36I CC'TnA_GG
Cuts at: 0 13 217 1569
Size: 13 204 1352
Fragments arranged by size:
1352 204 13
BtsI GCAGTG_nn'
Cuts at: 0 164 758 1569
Size: 164 594 811
Fragments arranged by size:
811 594 164
EaeI y'GGCC_r
Cuts at: 0 54 536 629 1569
Size: 54 482 93 940
Fragments arranged by size:
940 482 93 54
EarI CTCTTCn'nnn_
Cuts at: 0 655 1569
Size: 655 914
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 556 1569
Size: 556 1013
EcoICRI GAG'CTC
Cuts at: 0 444 1569
Size: 444 1125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 415 460 469 592 901 1060 1063
Size: 304 111 45 9 123 309 159 3
Cuts at: 1063 1426 1569
Size: 363 143
Fragments arranged by size:
363 309 304 159 143 123 111 45
9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 451 574 781 1009 1138 1286 1569
Size: 451 123 207 228 129 148 283
Fragments arranged by size:
451 283 228 207 148 129 123
EcoO109I rG'GnC_Cy
Cuts at: 0 338 543 597 786 974 1131 1492 1569
Size: 338 205 54 189 188 157 361 77
Fragments arranged by size:
361 338 205 189 188 157 77 54
EcoRI G'AATT_C
Cuts at: 0 1057 1569
Size: 1057 512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 477 1569
Size: 477 1092
HaeII r_GCGC'y
Cuts at: 0 10 391 1110 1569
Size: 10 381 719 459
Fragments arranged by size:
719 459 381 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 635 667 1058 1090 1206 1234 1238 1266
Size: 635 32 391 32 116 28 4 28
Cuts at: 1266 1297 1329 1569
Size: 31 32 240
Fragments arranged by size:
635 391 240 116 32 32 32 31
28 28 4
KasI G'GCGC_C
Cuts at: 0 1106 1569
Size: 1106 463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 902 1569
Size: 341 561 667
Fragments arranged by size:
667 561 341
MscI TGG'CCA
Cuts at: 0 538 631 1569
Size: 538 93 938
Fragments arranged by size:
938 538 93
MslI CAynn'nnrTG
Cuts at: 0 152 1569
Size: 152 1417
MspA1I CmG'CkG
Cuts at: 0 262 357 435 1080 1113 1161 1302 1569
Size: 262 95 78 645 33 48 141 267
Fragments arranged by size:
645 267 262 141 95 78 48 33
NarI GG'CG_CC
Cuts at: 0 1107 1569
Size: 1107 462
NspI r_CATG'y
Cuts at: 0 282 1569
Size: 282 1287
PasI CC'CwG_GG
Cuts at: 0 789 1569
Size: 789 780
PflMI CCAn_nnn'nTGG
Cuts at: 0 438 534 600 714 789 1083 1116 1557
Size: 438 96 66 114 75 294 33 441
Cuts at: 1557 1569
Size: 12
Fragments arranged by size:
441 438 294 114 96 75 66 33
12
PfoI T'CCnGG_A
Cuts at: 0 593 878 1569
Size: 593 285 691
Fragments arranged by size:
691 593 285
PmlI CAC'GTG
Cuts at: 0 729 1569
Size: 729 840
PpuMI rG'GwC_Cy
Cuts at: 0 543 597 974 1131 1492 1569
Size: 543 54 377 157 361 77
Fragments arranged by size:
543 377 361 157 77 54
PshAI GACnn'nnGTC
Cuts at: 0 1012 1569
Size: 1012 557
PspOMI G'GGCC_C
Cuts at: 0 337 1569
Size: 337 1232
PstI C_TGCA'G
Cuts at: 0 29 1130 1241 1287 1569
Size: 29 1101 111 46 282
Fragments arranged by size:
1101 282 111 46 29
PvuII CAG'CTG
Cuts at: 0 1113 1161 1302 1569
Size: 1113 48 141 267
Fragments arranged by size:
1113 267 141 48
SacI G_AGCT'C
Cuts at: 0 446 1569
Size: 446 1123
SapI GCTCTTCn'nnn_
Cuts at: 0 655 1569
Size: 655 914
SbfI CC_TGCA'GG
Cuts at: 0 1241 1569
Size: 1241 328
SexAI A'CCwGG_T
Cuts at: 0 599 1569
Size: 599 970
SfcI C'TryA_G
Cuts at: 0 25 1126 1237 1283 1499 1569
Size: 25 1101 111 46 216 70
Fragments arranged by size:
1101 216 111 70 46 25
SfoI GGC'GCC
Cuts at: 0 1108 1569
Size: 1108 461
SspI AAT'ATT
Cuts at: 0 270 1569
Size: 270 1299
StuI AGG'CCT
Cuts at: 0 39 460 1147 1569
Size: 39 421 687 422
Fragments arranged by size:
687 422 421 39
StyI C'CwwG_G
Cuts at: 0 539 1520 1569
Size: 539 981 49
Fragments arranged by size:
981 539 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 142 1569
Size: 142 1427
TatI w'GTAC_w
Cuts at: 0 173 1439 1569
Size: 173 1266 130
Fragments arranged by size:
1266 173 130
Tth111I GACn'n_nGTC
Cuts at: 0 601 649 1220 1569
Size: 601 48 571 349
Fragments arranged by size:
601 571 349 48
XbaI T'CTAG_A
Cuts at: 0 1356 1569
Size: 1356 213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 684 1344 1569
Size: 684 660 225
Fragments arranged by size:
684 660 225
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AleI AlfI AlwNI
ApaI ApoI BamHI BanI BanII BbeI BbsI BclI
BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstEII
BstYI BstZ17I Bsu36I BtsI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI
MmeI MscI MslI MspA1I NarI NspI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI
SapI SbfI SexAI SfcI SfoI SspI StuI StyI
TaqII TatI Tth111I XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AloI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BbvCI BcgI
BciVI BfrBI BlpI BmgBI BmtI BpuEI BsaBI BsiEI
BsiWI BsmI BspEI BsrBI BsrFI BssHII BssSI BstBI
BstXI BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoRV FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI TstI XhoI XmaI XmnI ZraI