Restriction map for pF1KSDA1960
 (Circular) (Six-base) MAPSORT of: pF1KSDA1960.seq  Check: 1635  from: 1  to: 5695



pF1KSDA1960  5695 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:36  ..

AatII G_ACGT'C

Cuts at:    595     595 
   Size:       5695
 
AccI GT'mk_AC

Cuts at:   2731    2731 
   Size:       5695
 
Acc65I G'GTAC_C

Cuts at:   2709    2709 
   Size:       5695
 
AclI AA'CG_TT

Cuts at:   5535    5535 
   Size:       5695
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    544    1002    1031    2095    2431    2548    3518    3950    4699 
   Size:        458      29    1064     336     117     970     432     749

Cuts at:   4699    4967     544 
   Size:        268    1272

  Fragments arranged by size: 

                1272    1064     970     749     458     432     336     268
                 117      29
 
AfeI AGC'GCT

Cuts at:   1044    1044 
   Size:       5695
 
AflIII A'CryG_T

Cuts at:   3045    4151    5128    3045 
   Size:       1106     977    3612

  Fragments arranged by size: 

                3612    1106     977
 
AgeI A'CCGG_T

Cuts at:   2469    4083    2469 
   Size:       1614    4081
 
AhdI GACnn_n'nnGTC

Cuts at:    993     993 
   Size:       5695
 
AleI CACnn'nnGTG

Cuts at:   2157    2157 
   Size:       5695
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    640    1497     640 
   Size:        857    4838
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    580     612     973    1005     580 
   Size:         32     361      32    5270

  Fragments arranged by size: 

                5270     361      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    748    1465    1770    1965    2220    2998    4567     748 
   Size:        717     305     195     255     778    1569    1876

  Fragments arranged by size: 

                1876    1569     778     717     305     255     195
 
ApaI G_GGCC'C

Cuts at:    340     890    1358     340 
   Size:        550     468    4677

  Fragments arranged by size: 

                4677     550     468
 
ApaLI G'TGCA_C

Cuts at:   4465    4465 
   Size:       5695
 
ApoI r'AATT_y

Cuts at:   2697    5048    5304    2697 
   Size:       2351     256    3088

  Fragments arranged by size: 

                3088    2351     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5695
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5695
 
AvaI C'yCGr_G

Cuts at:    253     333     426     435     534     699     728     889     955 
   Size:         80      93       9      99     165      29     161      66

Cuts at:    955    1750    1976    2318    2713     253 
   Size:        795     226     342     395    3235

  Fragments arranged by size: 

                3235     795     395     342     226     165     161      99
                  93      80      66      29       9
 
BamHI G'GATC_C

Cuts at:   2718    2718 
   Size:       5695
 
BanI G'GyrC_C

Cuts at:   2168    2709    3123    3371    3406    2168 
   Size:        541     414     248      35    4457

  Fragments arranged by size: 

                4457     541     414     248      35
 
BanII G_rGCy'C

Cuts at:    340     614     735     890     896    1358    2045    2707    3737 
   Size:        274     121     155       6     462     687     662    1030

Cuts at:   3737     340 
   Size:       2298

  Fragments arranged by size: 

                2298    1030     687     662     462     274     155     121
                   6
 
BbeI G_GCGC'C

Cuts at:   2172    3127    3375    2172 
   Size:        955     248    4492

  Fragments arranged by size: 

                4492     955     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   1202    3591    4354    5695    1202 
   Size:       2389     763    1341    1202

  Fragments arranged by size: 

                2389    1341    1202     763
 
BclI T'GATC_A

Cuts at:   3213    3213 
   Size:       5695
 
BglI GCCn_nnn'nGGC

Cuts at:    163     538     701    1266    1734    2034    2323    2503    2524 
   Size:        375     163     565     468     300     289     180      21

Cuts at:   2524     163 
   Size:       3334

  Fragments arranged by size: 

                3334     565     468     375     300     289     180     163
                  21
 
BglII A'GATC_T

Cuts at:   3208    5247    3208 
   Size:       2039    3656
 
BlpI GC'TnA_GC

Cuts at:    691    2805     691 
   Size:       2114    3581
 
Bme1580I G_kGCm'C

Cuts at:    340     890    1358    1708    2211    3318    3411    4469     340 
   Size:        550     468     350     503    1107      93    1058    1566

  Fragments arranged by size: 

                1566    1107    1058     550     503     468     350      93
            
 
BmrI ACTGGGnnnn_n'

Cuts at:    312    1550    2905    2986    3321    5467     312 
   Size:       1238    1355      81     335    2146     540

  Fragments arranged by size: 

                2146    1355    1238     540     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    882    2370    4060     882 
   Size:       1488    1690    2517

  Fragments arranged by size: 

                2517    1690    1488
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    852    1655    1925    2203    2242    2633     852 
   Size:        803     270     278      39     391    3914

  Fragments arranged by size: 

                3914     803     391     278     270      39
 
Bpu10I CC'TnA_GC

Cuts at:   1401    1401 
   Size:       5695
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2869    3748    4242    4540    2869 
   Size:        879     494     298    4024

  Fragments arranged by size: 

                4024     879     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1240    1245    5399    1240 
   Size:          5    4154    1536

  Fragments arranged by size: 

                4154    1536       5
 
BsaAI yAC'GTr

Cuts at:    312    2421    2685    3046     312 
   Size:       2109     264     361    2961

  Fragments arranged by size: 

                2961    2109     361     264
 
BsaBI GATnn'nnATC

Cuts at:      6    3207    5246       6 
   Size:       3201    2039     455

  Fragments arranged by size: 

                3201    2039     455
 
BsaHI Gr'CG_yC

Cuts at:    592    2169    2399    2579    3124    3372    4076    5572     592 
   Size:       1577     230     180     545     248     704    1496     715

  Fragments arranged by size: 

                1577    1496     715     704     545     248     230     180
            
 
BsaWI w'CCGG_w

Cuts at:   2469    2882    3403    4083    4357    4504    4915    2469 
   Size:        413     521     680     274     147     411    3249

  Fragments arranged by size: 

                3249     680     521     413     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    142     172     580     610     664     694     973    1003    2360 
   Size:         30     408      30      54      30     279      30    1357

Cuts at:   2360    2390    2631    2661     142 
   Size:         30     241      30    3176

  Fragments arranged by size: 

                3176    1357     408     279     241      54      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1612    1768    1924    1964    1996    2407    2491    1612 
   Size:        156     156      40      32     411      84    4816

  Fragments arranged by size: 

                4816     411     156     156      84      40      32
 
BseYI C'CCAG_C

Cuts at:    520     651    1359    1635    1811    1816    1958    2280    2528 
   Size:        131     708     276     176       5     142     322     248

Cuts at:   2528    2617    3118    3876    4455    4951     520 
   Size:         89     501     758     579     496    1264

  Fragments arranged by size: 

                1264     758     708     579     501     496     322     276
                 248     176     142     131      89       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2033    2033 
   Size:       5695
 
BsiEI CG_ry'CG

Cuts at:    104     153     485     756    3235    3281    4491     104 
   Size:         49     332     271    2479      46    1210    1308

  Fragments arranged by size: 

                2479    1308    1210     332     271      49      46
 
BsiHKAI G_wGCw'C

Cuts at:    584     614    2420    2466    2707    3485    3675    4469     584 
   Size:         30    1806      46     241     778     190     794    1810

  Fragments arranged by size: 

                1810    1806     794     778     241     190      46      30
            
 
BsmI GAATG_Cn'

Cuts at:    853     853 
   Size:       5695
 
BsmBI CGTCTCn'nnnn_

Cuts at:    583     952    2618     583 
   Size:        369    1666    3660

  Fragments arranged by size: 

                3660    1666     369
 
Bsp1286I G_dGCh'C

Cuts at:    340     584     614     735     890     896    1358    1708    2045 
   Size:        244      30     121     155       6     462     350     337

Cuts at:   2045    2211    2420    2466    2707    3318    3411    3485    3675 
   Size:        166     209      46     241     611      93      74     190

Cuts at:   3675    3737    4469     340 
   Size:         62     732    1566

  Fragments arranged by size: 

                1566     732     611     462     350     337     244     241
                 209     190     166     155     121      93      74      62
                  46      30       6
 
BspEI T'CCGG_A

Cuts at:   4915    4915 
   Size:       5695
 
BspHI T'CATG_A

Cuts at:   4871    4871 
   Size:       5695
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1258    2743    2957    3259    3640    1258 
   Size:       1485     214     302     381    3313

  Fragments arranged by size: 

                3313    1485     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1446    2136    2226    2436    3985    4039    5525    5690 
   Size:       1396     690      90     210    1549      54    1486     165

Cuts at:   5690      50 
   Size:         55

  Fragments arranged by size: 

                1549    1486    1396     690     210     165      90      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:   1668    1886    3605    1668 
   Size:        218    1719    3758

  Fragments arranged by size: 

                3758    1719     218
 
BsrFI r'CCGG_y

Cuts at:    528     717     751     837    1968    2234    2469    3691    3872 
   Size:        189      34      86    1131     266     235    1222     181

Cuts at:   3872    4083     528 
   Size:        211    2140

  Fragments arranged by size: 

                2140    1222    1131     266     235     211     189     181
                  86      34
 
BssHII G'CGCG_C

Cuts at:    207     566     721    2296     207 
   Size:        359     155    1575    3606

  Fragments arranged by size: 

                3606    1575     359     155
 
BssSI C'ACGA_G

Cuts at:    937    3964    4324     937 
   Size:       3027     360    2308

  Fragments arranged by size: 

                3027    2308     360
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3070    3070 
   Size:       5695
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       5695
 
BstXI CCAn_nnnn'nTGG

Cuts at:    984    1390    1414    2032     984 
   Size:        406      24     618    4647

  Fragments arranged by size: 

                4647     618     406      24
 
BstYI r'GATC_y

Cuts at:      1    1782    2718    3185    3208    3543    3789    4803    4889 
   Size:       1781     936     467      23     335     246    1014      86

Cuts at:   4889    4901    5247       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1781    1014     936     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   2213    2213 
   Size:       5695
 
BtgI C'CryG_G

Cuts at:    125     373     386     497     125 
   Size:        248      13     111    5323

  Fragments arranged by size: 

                5323     248     111      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3643    3824    3987    3643 
   Size:        181     163    5351

  Fragments arranged by size: 

                5351     181     163
 
BtsI GCAGTG_nn'

Cuts at:    408    2198    2936     408 
   Size:       1790     738    3167

  Fragments arranged by size: 

                3167    1790     738
 
DraI TTT'AAA

Cuts at:   2692    2692 
   Size:       5695
 
DraIII CAC_nnn'GTG

Cuts at:   2016    2016 
   Size:       5695
 
DrdI GACnn_nn'nnGTC

Cuts at:   3399    4259    3399 
   Size:        860    4835
 
EaeI y'GGCC_r

Cuts at:    482     753    1416    1710    1772    2087    2243    2598    3278 
   Size:        271     663     294      62     315     156     355     680

Cuts at:   3278    3452    3843    3870    5217     482 
   Size:        174     391      27    1347     960

  Fragments arranged by size: 

                1347     960     680     663     391     355     315     294
                 271     174     156      62      27
 
EagI C'GGCC_G

Cuts at:    482     753    3278     482 
   Size:        271    2525    2899

  Fragments arranged by size: 

                2899    2525     271
 
EarI CTCTTCn'nnn_

Cuts at:   1295    2675    3716    3926    1295 
   Size:       1380    1041     210    3064

  Fragments arranged by size: 

                3064    1380    1041     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    505     672    2545    3901    4213    4359    5505     505 
   Size:        167    1873    1356     312     146    1146     695

  Fragments arranged by size: 

                1873    1356    1146     695     312     167     146
 
EcoICRI GAG'CTC

Cuts at:    612    2705     612 
   Size:       2093    3602
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    544     852    1002    1031    1655    1925    2095    2203    2242 
   Size:        308     150      29     624     270     170     108      39

Cuts at:   2242    2431    2548    2633    3518    3950    4699    4967     544 
   Size:        189     117      85     885     432     749     268    1272

  Fragments arranged by size: 

                1272     885     749     624     432     308     270     268
                 189     170     150     117     108      85      39      29
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    856    1300    2563     856 
   Size:        444    1263    3988

  Fragments arranged by size: 

                3988    1263     444
 
EcoO109I rG'GnC_Cy

Cuts at:    132     139     337     423     431     760     995    1305    1354 
   Size:          7     198      86       8     329     235     310      49

Cuts at:   1354    1355    1675    1937    2115    2639    2832     132 
   Size:          1     320     262     178     524     193    2995

  Fragments arranged by size: 

                2995     524     329     320     310     262     235     198
                 193     178      86      49       8       7       1
 
EcoRI G'AATT_C

Cuts at:   2697    2697 
   Size:       5695
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    807    3712     807 
   Size:       2905    2790
 
FspI TGC'GCA

Cuts at:   3474    3474 
   Size:       5695
 
HaeII r_GCGC'y

Cuts at:    919    1046    2172    3127    3375    4399    5378     919 
   Size:        127    1126     955     248    1024     979    1236

  Fragments arranged by size: 

                1236    1126    1024     979     955     248     127
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    142     174     979    1011    1578    1610    1921    1953    2360 
   Size:         32     805      32     567      32     311      32     407

Cuts at:   2360    2392    2631    2663    3535    3565    3567    3597     142 
   Size:         32     239      32     872      30       2      30    2240

  Fragments arranged by size: 

                2240     872     805     567     407     311     239      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   2732    2732 
   Size:       5695
 
KasI G'GCGC_C

Cuts at:   2168    3123    3371    2168 
   Size:        955     248    4492

  Fragments arranged by size: 

                4492     955     248
 
KpnI G_GTAC'C

Cuts at:   2713    2713 
   Size:       5695
 
MluI A'CGCG_T

Cuts at:   5128    5128 
   Size:       5695
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    924    1012    1018    4366    4550     924 
   Size:         88       6    3348     184    2069

  Fragments arranged by size: 

                3348    2069     184      88       6
 
MscI TGG'CCA

Cuts at:   1418    1712    1774    2600    3454    1418 
   Size:        294      62     826     854    3659

  Fragments arranged by size: 

                3659     854     826     294      62
 
MslI CAynn'nnrTG

Cuts at:   2157    3682    3809    2157 
   Size:       1525     127    4043

  Fragments arranged by size: 

                4043    1525     127
 
MspA1I CmG'CkG

Cuts at:    375     388     465     499     555     573     780    1606    1639 
   Size:         13      77      34      56      18     207     826      33

Cuts at:   1639    2385    2804    3118    3478    4493    4738     375 
   Size:        746     419     314     360    1015     245    1332

  Fragments arranged by size: 

                1332    1015     826     746     419     360     314     245
                 207      77      56      34      33      18      13
 
NaeI GCC'GGC

Cuts at:    530     719     753     839    1970    3874     530 
   Size:        189      34      86    1131    1904    2351

  Fragments arranged by size: 

                2351    1904    1131     189      86      34
 
NarI GG'CG_CC

Cuts at:   2169    3124    3372    2169 
   Size:        955     248    4492

  Fragments arranged by size: 

                4492     955     248
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       5695
 
NgoMIV G'CCGG_C

Cuts at:    528     717     751     837    1968    3872     528 
   Size:        189      34      86    1131    1904    2351

  Fragments arranged by size: 

                2351    1904    1131     189      86      34
 
NruI TCG'CGA

Cuts at:   4056    4056 
   Size:       5695
 
NspI r_CATG'y

Cuts at:   2746    3246    4155    2746 
   Size:        500     909    4286

  Fragments arranged by size: 

                4286     909     500
 
PasI CC'CwG_GG

Cuts at:    135     883    1350    1833    2635     135 
   Size:        748     467     483     802    3195

  Fragments arranged by size: 

                3195     802     748     483     467
 
PciI A'CATG_T

Cuts at:   4151    4151 
   Size:       5695
 
PfoI T'CCnGG_A

Cuts at:    584    1458     584 
   Size:        874    4821
 
PmeI GTTT'AAAC

Cuts at:   2692    2692 
   Size:       5695
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    580     612     973    1005    4834    4866     580 
   Size:         32     361      32    3829      32    1409

  Fragments arranged by size: 

                3829    1409     361      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    139     423     431     760     995    1675    1937    2115    2639 
   Size:        284       8     329     235     680     262     178     524

Cuts at:   2639     139 
   Size:       3195

  Fragments arranged by size: 

                3195     680     524     329     284     262     235     178
                   8
 
PspOMI G'GGCC_C

Cuts at:    336     886    1354     336 
   Size:        550     468    4677

  Fragments arranged by size: 

                4677     550     468
 
PspXI vC'TCGA_Gb

Cuts at:    534    1750     534 
   Size:       1216    4479
 
PstI C_TGCA'G

Cuts at:   1200    1470    1611    2144    2193    2366    2384    2740    3425 
   Size:        270     141     533      49     173      18     356     685

Cuts at:   3425    1200 
   Size:       3470

  Fragments arranged by size: 

                3470     685     533     356     270     173     141      49
                  18
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5695
 
PvuII CAG'CTG

Cuts at:   1606    1639    3118    3478    1606 
   Size:         33    1479     360    3823

  Fragments arranged by size: 

                3823    1479     360      33
 
RsrII CG'GwC_CG

Cuts at:   3888    3888 
   Size:       5695
 
SacI G_AGCT'C

Cuts at:    614    2707     614 
   Size:       2093    3602
 
SacII CC_GC'GG

Cuts at:    376     389     500     376 
   Size:         13     111    5571

  Fragments arranged by size: 

                5571     111      13
 
SalI G'TCGA_C

Cuts at:   2730    2730 
   Size:       5695
 
SanDI GG'GwC_CC

Cuts at:    431     760     431 
   Size:        329    5366
 
SapI GCTCTTCn'nnn_

Cuts at:   3716    3926    3716 
   Size:        210    5485
 
SbfI CC_TGCA'GG

Cuts at:   2740    2740 
   Size:       5695
 
ScaI AGT'ACT

Cuts at:   1923    5212    1923 
   Size:       3289    2406
 
SfcI C'TryA_G

Cuts at:     33     222    1196    1466    1505    1607    2140    2189    2362 
   Size:        189     974     270      39     102     533      49     173

Cuts at:   2362    2380    2736    3421    4416    4607      33 
   Size:         18     356     685     995     191    1121

  Fragments arranged by size: 

                1121     995     974     685     533     356     270     191
                 189     173     102      49      39      18
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    538    1734    2323     538 
   Size:       1196     589    3910

  Fragments arranged by size: 

                3910    1196     589
 
SfoI GGC'GCC

Cuts at:   2170    3125    3373    2170 
   Size:        955     248    4492

  Fragments arranged by size: 

                4492     955     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5695
 
SmaI CCC'GGG

Cuts at:    335     428     437     701     730    2320    2715     335 
   Size:         93       9     264      29    1590     395    3315

  Fragments arranged by size: 

                3315    1590     395     264      93      29       9
 
SmlI C'TyrA_G

Cuts at:    534    1750    2848    3763    4257    4519     534 
   Size:       1216    1098     915     494     262    1710

  Fragments arranged by size: 

                1710    1216    1098     915     494     262
 
SnaBI TAC'GTA

Cuts at:   2685    2685 
   Size:       5695
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5695
 
SphI G_CATG'C

Cuts at:   2746    3246    2746 
   Size:        500    5195
 
SrfI GCCC'GGGC

Cuts at:    335    2320     335 
   Size:       1985    3710
 
StuI AGG'CCT

Cuts at:    862    2203    2353     862 
   Size:       1341     150    4204

  Fragments arranged by size: 

                4204    1341     150
 
StyI C'CwwG_G

Cuts at:    125     896     969    1113    1646    2402    2595    2827    3181 
   Size:        771      73     144     533     756     193     232     354

Cuts at:   3181     125 
   Size:       2639

  Fragments arranged by size: 

                2639     771     756     533     354     232     193     144
                  73
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    844    1972    2023    3276    3409    3868    4079     844 
   Size:       1128      51    1253     133     459     211    2460

  Fragments arranged by size: 

                2460    1253    1128     459     211     133      51
 
TatI w'GTAC_w

Cuts at:   1921    5210    1921 
   Size:       3289    2406
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    664     696    5212    5244     664 
   Size:         32    4516      32    1115

  Fragments arranged by size: 

                4516    1115      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3490    3490 
   Size:       5695
 
XbaI T'CTAG_A

Cuts at:   2724    2724 
   Size:       5695
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    132     180    1537    3188     132 
   Size:         48    1357    1651    2639

  Fragments arranged by size: 

                2639    1651    1357      48
 
XhoI C'TCGA_G

Cuts at:    534    1750     534 
   Size:       1216    4479
 
XmaI C'CCGG_G

Cuts at:    333     426     435     699     728    2318    2713     333 
   Size:         93       9     264      29    1590     395    3315

  Fragments arranged by size: 

                3315    1590     395     264      93      29       9
 
ZraI GAC'GTC

Cuts at:    593     593 
   Size:       5695
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NruI     NspI     PasI     PciI
     PfoI     PmeI     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI    AflII     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI
    BfrBI    BmgBI     BmtI    BsiWI    BsrGI   BstEII  BstZ17I     ClaI
    CspCI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NsiI     PacI    PflMI     PmlI    PshAI     PsiI
     PsrI    SexAI    SgrAI     SspI     SwaI     XmnI
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