Restriction map for pF1KSDA1869
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AviII       AvrII       
BbrPI       BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      Eam1105I    Eco47III    Eco81I      EcoO65I     
EcoRI       EcoT22I     FbaI        FspI        HincII      
HindII      HpaI        KpnI        KspI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tsp509I     XbaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII       2058  TCCGGA               T^CCGGA
Asp700I       423  GAACTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          700  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1111  GGATCC               G^GATCC
BbeI         1849  GGCGCC               GGCGC^C
BbsI          952  GTCTTC               GAAGAC(2/6)
BbvCI        2451  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1910  GCAAGGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         952  GTCTTC               GAAGAC(2/6)
BsaBI        1663  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2058  TCCGGA               T^CCGGA
BsiEI        2775  CGGCCG               CG[GA][CT]^CG
BspEI        2058  TCCGGA               T^CCGGA
BssSI        2434  CACGAG               CACGAG(-5/-1)
BstAPI       1834  GCAGCTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI         1071  GACTCCTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2775  CGGCCG               C^GGCCG
EclXI        2775  CGGCCG               C^GGCCG
Eco52I       2775  CGGCCG               C^GGCCG
EcoRV        1639  GATATC               GAT^ATC
FseI         2659  GGCCGGCC             GGCCGG^CC
HindIII       844  AAGCTT               A^AGCTT
KasI         1849  GGCGCC               G^GCGCC
MamI         1663  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2058  TCCGGA               T^CCGGA
MseI         2368  TTAA                 T^TAA
MslI         1314  CACAGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         2660  GCCGGC               GCC^GGC
NarI         1849  GGCGCC               GG^CGCC
NcoI         1691  CCATGG               C^CATGG
NgoMIV       2660  GCCGGC               G^CCGGC
PaeR7I       2594  CTCGAG               C^TCGAG
PflFI         700  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         569  CAGCTG               CAG^CTG
SfiI         1821  GGCCAGTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1849  GGCGCC               GGC^GCC
SmlI         2594  CTCGAG               C^T[CT][GA]AG
Tru9I        2368  TTAA                 T^TAA
Tth111I       700  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2594  CTCGAG               C^TCGAG
XmnI          423  GAACTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AatII        1901  GACGTC               GACGT^C
             2701  GACGTC              
AccI          706  GTCTAC               GT^[AC][GT]AC
             1534  GTCTAC              
AlwNI        1626  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1856  CAGTCTCTG           
BalI          533  TGGCCA               TGG^CCA
             1820  TGGCCA              
BanI          285  GGTGCC               G^G[CT][GA]CC
             1849  GGCGCC              
BfaI          220  CTAG                 C^TAG
             1253  CTAG                
BglI         1822  GCCAGTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2448  GCCGCTGAGGC         
BsaWI         209  ACCGGA               [AT]^CCGG[AT]
             2058  TCCGGA              
BstXI         127  CCATTATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1394  CCATCCTCCTGG        
BstYI        1111  GGATCC               [GA]^GATC[CT]
             1648  GGATCT              
EarI         1149  CTCTTC               CTCTTC(1/4)
             1405  GAAGAG              
EcoNI        1097  CCTCACCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1571  CCTGTCAGAGG         
HaeII        1849  GGCGCC               [GA]GCGC^[CT]
             2379  GGCGCT              
Ksp632I      1149  CTCTTC               CTCTTC(1/4)
             1405  GAAGAG              
MaeI          220  CTAG                 C^TAG
             1253  CTAG                
MflI         1111  GGATCC               [GA]^GATC[CT]
             1648  GGATCT              
MluNI         533  TGGCCA               TGG^CCA
             1820  TGGCCA              
MscI          533  TGGCCA               TGG^CCA
             1820  TGGCCA              
PstI          167  CTGCAG               CTGCA^G
              466  CTGCAG              
StuI          683  AGGCCT               AGG^CCT
              902  AGGCCT              
XcmI         1603  CCATACTGTGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1625  CCAGGGGCTGTGTGG     
XhoII        1111  GGATCC               [GA]^GATC[CT]
             1648  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AvaI        AvaII       BanII       
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XmaCI       XmaI        
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