Restriction map for pF1KSDA1852
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AciI        
AclI        AflII       AflIII      AgeI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbeI        
BbvCI       BcgI        BcnI        BfaI        BfrI        
BglII       BlnI        BsaBI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstXI       BstZ17I     CfoI        Cfr10I      
ClaI        CpoI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoRV       FauI        
FseI        FspI        HaeII       HgaI        HhaI        
HinP1I      HpaI        KasI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        1218  GACGTC               GACGT^C
AcyI         1218  GACGTC               G[GA]^CG[CT]C
AhdI          279  GACCTATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          322  CCTGAGG              CC^T[ACGT]AGG
AspEI         279  GACCTATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI           31  GGTGCC               G^G[CT][GA]CC
BbrPI         115  CACGTG               CAC^GTG
BbsI          592  GTCTTC               GAAGAC(2/6)
BciVI         102  GTATCC               GTATCC(6/5)
BclI          915  TGATCA               T^GATCA
BglI           79  GCCTCCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         592  GTCTTC               GAAGAC(2/6)
BsaAI         115  CACGTG               [CT]AC^GT[GA]
BsaHI        1218  GACGTC               G[GA]^CG[CT]C
BsaI          277  GAGACC               GGTCTC(1/5)
BsaWI        2093  ACCGGA               [AT]^CCGG[AT]
BsmAI         277  GAGAC                GTCTC(1/5)
BspMI         290  ACCTGC               ACCTGC(4/8)
BsrDI        2085  CATTGC               GCAATG(2/0)
BstYI        2059  AGATCC               [GA]^GATC[CT]
Bsu36I        322  CCTGAGG              CC^T[ACGT]AGG
Cac8I         372  GCAGGC               GC[ACGT]^[ACGT]GC
DraI         1618  TTTAAA               TTT^AAA
DraII         250  AGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          200  TGGCCG               [CT]^GGCC[GA]
Eam1105I      279  GACCTATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        322  CCTGAGG              CC^T[ACGT]AGG
EcoNI         129  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      250  AGGACCC              [GA]G^G[ACGT]CC[CT]
FbaI          915  TGATCA               T^GATCA
HapII        2094  CCGG                 C^CGG
Hin1I        1218  GACGTC               G[GA]^CG[CT]C
HincII         67  GTTGAC               GT[CT]^[GA]AC
HindII         67  GTTGAC               GT[CT]^[GA]AC
HindIII      1640  AAGCTT               A^AGCTT
HpaII        2094  CCGG                 C^CGG
MflI         2059  AGATCC               [GA]^GATC[CT]
MspI         2094  CCGG                 C^CGG
NdeI         1588  CATATG               CA^TATG
PmaCI         115  CACGTG               CAC^GTG
PmlI          115  CACGTG               CAC^GTG
PpuMI         250  AGGACCC              [GA]G^G[AT]CC[CT]
PstI         1184  CTGCAG               CTGCA^G
SapI          352  GAAGAGC              GCTCTTC(1/4)
ScaI          609  AGTACT               AGT^ACT
TfiI         1314  GAATC                G^A[AT]TC
XcmI           73  CCATGGGCCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        2059  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BlpI         1319  GCTCAGC              GC^T[ACGT]AGC
             1823  GCTCAGC             
Bpu1102I     1319  GCTCAGC              GC^T[ACGT]AGC
             1823  GCTCAGC             
BseRI         133  GAGGAG               GAGGAG(10/8)
              141  GAGGAG              
BsmFI        1216  GGGAC                GGGAC(10/14)
             1649  GGGAC               
Bsp1407I     1416  TGTACA               T^GTACA
             2172  TGTACA              
BsrGI        1416  TGTACA               T^GTACA
             2172  TGTACA              
BstEII        286  GGTCACC              G^GT[ACGT]ACC
              774  GGTTACC             
BstPI         286  GGTCACC              G^GT[ACGT]ACC
              774  GGTTACC             
CelII        1319  GCTCAGC              GC^T[ACGT]AGC
             1823  GCTCAGC             
EcoO65I       286  GGTCACC              G^GT[ACGT]ACC
              774  GGTTACC             
EcoT22I      1597  ATGCAT               ATGCA^T
             2269  ATGCAT              
MwoI           79  GCCTCCTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             2288  GCTTTATATGC         
NcoI           73  CCATGG               C^CATGG
              468  CCATGG              
NsiI         1597  ATGCAT               ATGCA^T
             2269  ATGCAT              
NspI          376  GCATGT               [GA]CATG^[CT]
              726  ACATGC              
SfaNI         270  GATGC                GCATC(5/9)
              877  GATGC               
SspBI        1416  TGTACA               T^GTACA
             2172  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     AspHI       AvaII       BanII       BbvI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
DsaI        EarI        EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MvaI        NdeII       NlaIII      NlaIV       PleI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfcI        StyI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XmnI        
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