Restriction map for pF1KSDA1852
 (Linear) (Six-base) MAPSORT of: KIAA1852.seq  Check: 6271  from: 1  to: 2376



KIAA1852.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     299    2376 
   Size:        299    2077
 
AatII G_ACGT'C

Cuts at:      0    1222    2376 
   Size:       1222    1154
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1377    1713    1965    2376 
   Size:       1377     336     252     411

  Fragments arranged by size: 

                1377     411     336     252
 
AhdI GACnn_n'nnGTC

Cuts at:      0     284    2376 
   Size:        284    2092
 
AleI CACnn'nnGTG

Cuts at:      0    2024    2376 
   Size:       2024     352
 
ApoI r'AATT_y

Cuts at:      0     631     926    1401    1427    2015    2241    2376 
   Size:        631     295     475      26     588     226     135

  Fragments arranged by size: 

                 631     588     475     295     226     135      26
 
BanI G'GyrC_C

Cuts at:      0      31    2376 
   Size:         31    2345
 
BanII G_rGCy'C

Cuts at:      0      90     158     988    1237    1573    1741    2376 
   Size:         90      68     830     249     336     168     635

  Fragments arranged by size: 

                 830     635     336     249     168      90      68
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     585    2376 
   Size:        585    1791
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     113    2376 
   Size:        113    2263
 
BclI T'GATC_A

Cuts at:      0     915    2376 
   Size:        915    1461
 
BfrBI ATG'CAT

Cuts at:      0    1599    2271    2376 
   Size:       1599     672     105

  Fragments arranged by size: 

                1599     672     105
 
BglI GCCn_nnn'nGGC

Cuts at:      0      85    2376 
   Size:         85    2291
 
BlpI GC'TnA_GC

Cuts at:      0    1320    1824    2376 
   Size:       1320     504     552

  Fragments arranged by size: 

                1320     552     504
 
Bme1580I G_kGCm'C

Cuts at:      0      36    2376 
   Size:         36    2340
 
BmgBI CAC'GTC

Cuts at:      0     198    2376 
   Size:        198    2178
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     161    2376 
   Size:        161    2215
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     348     710     961    2376 
   Size:        348     362     251    1415

  Fragments arranged by size: 

                1415     362     348     251
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     295     498     872    1251    1289    1587    1625 
   Size:        151     144     203     374     379      38     298      38

Cuts at:   1625    1709    1755    1961    2129    2213    2376 
   Size:         84      46     206     168      84     163

  Fragments arranged by size: 

                 379     374     298     206     203     168     163     151
                 144      84      84      46      38      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     271    2376 
   Size:        271    2105
 
BsaAI yAC'GTr

Cuts at:      0     117    2376 
   Size:        117    2259
 
BsaHI Gr'CG_yC

Cuts at:      0    1219    2376 
   Size:       1219    1157
 
BsaWI w'CCGG_w

Cuts at:      0    2093    2376 
   Size:       2093     283
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     156    2376 
   Size:        148       8    2220

  Fragments arranged by size: 

                2220     148       8
 
BseYI C'CCAG_C

Cuts at:      0     159     435    2376 
   Size:        159     276    1941

  Fragments arranged by size: 

                1941     276     159
 
BsiHKAI G_wGCw'C

Cuts at:      0    1237    1573    1741    2376 
   Size:       1237     336     168     635

  Fragments arranged by size: 

                1237     635     336     168
 
BsmI GAATG_Cn'

Cuts at:      0     967    1601    1810    1963    2273    2376 
   Size:        967     634     209     153     310     103

  Fragments arranged by size: 

                 967     634     310     209     153     103
 
Bsp1286I G_dGCh'C

Cuts at:      0      36      90     158     988    1237    1573    1741    2376 
   Size:         36      54      68     830     249     336     168     635

  Fragments arranged by size: 

                 830     635     336     249     168      68      54      36
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     299    2376 
   Size:        299    2077
 
BsrDI GCAATG_nn'

Cuts at:      0    2084    2376 
   Size:       2084     292
 
BsrGI T'GTAC_A

Cuts at:      0    1416    2172    2376 
   Size:       1416     756     204

  Fragments arranged by size: 

                1416     756     204
 
BstEII G'GTnAC_C

Cuts at:      0     286     774    2376 
   Size:        286     488    1602

  Fragments arranged by size: 

                1602     488     286
 
BstYI r'GATC_y

Cuts at:      0    2059    2376 
   Size:       2059     317
 
Bsu36I CC'TnA_GG

Cuts at:      0     323    2376 
   Size:        323    2053
 
BtgI C'CryG_G

Cuts at:      0      16      73     203     358     468    2376 
   Size:         16      57     130     155     110    1908

  Fragments arranged by size: 

                1908     155     130     110      57      16
 
BtsI GCAGTG_nn'

Cuts at:      0    1181    2304    2376 
   Size:       1181    1123      72

  Fragments arranged by size: 

                1181    1123      72
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     205     240    1194    1229    1950    1985    2376 
   Size:        205      35     954      35     721      35     391

  Fragments arranged by size: 

                 954     721     391     205      35      35      35
 
DraI TTT'AAA

Cuts at:      0    1620    2376 
   Size:       1620     756
 
EaeI y'GGCC_r

Cuts at:      0     200    2376 
   Size:        200    2176
 
EarI CTCTTCn'nnn_

Cuts at:      0     215     347    2357    2376 
   Size:        215     132    2010      19

  Fragments arranged by size: 

                2010     215     132      19
 
EcoICRI GAG'CTC

Cuts at:      0    1235    1571    1739    2376 
   Size:       1235     336     168     637

  Fragments arranged by size: 

                1235     637     336     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     295     498     872    1251    1289    1377    1587 
   Size:        151     144     203     374     379      38      88     210

Cuts at:   1587    1625    1709    1713    1755    1961    1965    2129    2213 
   Size:         38      84       4      42     206       4     164      84

Cuts at:   2213    2376 
   Size:        163

  Fragments arranged by size: 

                 379     374     210     206     203     164     163     151
                 144      88      84      84      42      38      38       4
                   4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     133    2376 
   Size:        133    2243
 
EcoO109I rG'GnC_Cy

Cuts at:      0     251    2376 
   Size:        251    2125
 
EcoRI G'AATT_C

Cuts at:      0     631     926    1401    1427    2015    2241    2376 
   Size:        631     295     475      26     588     226     135

  Fragments arranged by size: 

                 631     588     475     295     226     135      26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     270     302     346     378    1560    1592    1728    1760 
   Size:        270      32      44      32    1182      32     136      32

Cuts at:   1760    2146    2178    2376 
   Size:        386      32     198

  Fragments arranged by size: 

                1182     386     270     198     136      44      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0      69    2376 
   Size:         69    2307
 
HindIII A'AGCT_T

Cuts at:      0    1640    2376 
   Size:       1640     736
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     461     718     999    2376 
   Size:        320     141     257     281    1377

  Fragments arranged by size: 

                1377     320     281     257     141
 
MslI CAynn'nnrTG

Cuts at:      0     911    1704    2024    2124    2376 
   Size:        911     793     320     100     252

  Fragments arranged by size: 

                 911     793     320     252     100
 
MspA1I CmG'CkG

Cuts at:      0      64     234    1397    1565    1733    2376 
   Size:         64     170    1163     168     168     643

  Fragments arranged by size: 

                1163     643     170     168     168      64
 
NcoI C'CATG_G

Cuts at:      0      73     468    2376 
   Size:         73     395    1908

  Fragments arranged by size: 

                1908     395      73
 
NdeI CA'TA_TG

Cuts at:      0    1589    2376 
   Size:       1589     787
 
NsiI A_TGCA'T

Cuts at:      0    1601    2273    2376 
   Size:       1601     672     103

  Fragments arranged by size: 

                1601     672     103
 
NspI r_CATG'y

Cuts at:      0     380     730    2376 
   Size:        380     350    1646

  Fragments arranged by size: 

                1646     380     350
 
PmlI CAC'GTG

Cuts at:      0     117    2376 
   Size:        117    2259
 
PpuMI rG'GwC_Cy

Cuts at:      0     251    2376 
   Size:        251    2125
 
PstI C_TGCA'G

Cuts at:      0    1188    2376 
   Size:       1188    1188
 
PvuII CAG'CTG

Cuts at:      0      64     234    1397    1565    1733    2376 
   Size:         64     170    1163     168     168     643

  Fragments arranged by size: 

                1163     643     170     168     168      64
 
SacI G_AGCT'C

Cuts at:      0    1237    1573    1741    2376 
   Size:       1237     336     168     635

  Fragments arranged by size: 

                1237     635     336     168
 
SapI GCTCTTCn'nnn_

Cuts at:      0     347    2376 
   Size:        347    2029
 
ScaI AGT'ACT

Cuts at:      0     611    2376 
   Size:        611    1765
 
SfcI C'TryA_G

Cuts at:      0    1100    1184    1896    2070    2376 
   Size:       1100      84     712     174     306

  Fragments arranged by size: 

                1100     712     306     174      84
 
StyI C'CwwG_G

Cuts at:      0      73     468     505    1006    2376 
   Size:         73     395      37     501    1370

  Fragments arranged by size: 

                1370     501     395      73      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     229     393    1019    2376 
   Size:        229     164     626    1357

  Fragments arranged by size: 

                1357     626     229     164
 
TatI w'GTAC_w

Cuts at:      0     571     609     871    1416    1540    1708    2172    2376 
   Size:        571      38     262     545     124     168     464     204

  Fragments arranged by size: 

                 571     545     464     262     204     168     124      38
            
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      80    2376 
   Size:         80    2296
 
XmnI GAAnn'nnTTC

Cuts at:      0    1474    1558    1683    2376 
   Size:       1474      84     125     693

  Fragments arranged by size: 

                1474     693     125      84
 
ZraI GAC'GTC

Cuts at:      0    1220    2376 
   Size:       1220    1156
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AhdI     AleI     ApoI     BanI    BanII
     BbsI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BseRI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrGI   BstEII
    BstYI   Bsu36I     BtgI     BtsI    CspCI     DraI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I   HincII  HindIII
     MmeI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI     PmlI
    PpuMI     PstI    PvuII     SacI     SapI     ScaI     SfcI     StyI
    TaqII     TatI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BglII     BmtI
   Bpu10I    BpuEI    BsaBI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
    BstXI  BstZ17I    BtgZI     ClaI   DraIII     DrdI     EagI     EciI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
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