Restriction map for pF1KSDA1810
 (Circular) (Six-base) MAPSORT of: pF1KSDA1810.seq  Check: 8617  from: 1  to: 4832



pF1KSDA1810  4832 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    964     964 
   Size:       4832
 
AccI GT'mk_AC

Cuts at:   1870    1870 
   Size:       4832
 
Acc65I G'GTAC_C

Cuts at:   1848    1848 
   Size:       4832
 
AclI AA'CG_TT

Cuts at:    363    4672     363 
   Size:       4309     523
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    405    1568    2657    3089    3836    4104     405 
   Size:       1163    1089     432     747     268    1133

  Fragments arranged by size: 

                1163    1133    1089     747     432     268
 
AflIII A'CryG_T

Cuts at:   2184    3288    4265    2184 
   Size:       1104     977    2751

  Fragments arranged by size: 

                2751    1104     977
 
AgeI A'CCGG_T

Cuts at:   3220    3220 
   Size:       4832
 
AleI CACnn'nnGTG

Cuts at:    139     223     139 
   Size:         84    4748
 
AlwNI CAG_nnn'CTG

Cuts at:   1246    1282    1294    1312    1467    1488    1696    1720    1800 
   Size:         36      12      18     155      21     208      24      80

Cuts at:   1800    2137    3704    1246 
   Size:        337    1567    2374

  Fragments arranged by size: 

                2374    1567     337     208     155      80      36      24
                  21      18      12
 
ApaI G_GGCC'C

Cuts at:   1141    1568    1141 
   Size:        427    4405
 
ApaLI G'TGCA_C

Cuts at:    682    3602     682 
   Size:       2920    1912
 
ApoI r'AATT_y

Cuts at:   1836    4185    4441    1836 
   Size:       2349     256    2227

  Fragments arranged by size: 

                2349    2227     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4832
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4832
 
AvaI C'yCGr_G

Cuts at:   1852    1852 
   Size:       4832
 
BamHI G'GATC_C

Cuts at:   1857    1857 
   Size:       4832
 
BanI G'GyrC_C

Cuts at:    874    1249    1784    1848    2262    2510    2545     874 
   Size:        375     535      64     414     248      35    3161

  Fragments arranged by size: 

                3161     535     414     375     248      64      35
 
BanII G_rGCy'C

Cuts at:    824    1141    1443    1568    1763    1778    1846    2876     824 
   Size:        317     302     125     195      15      68    1030    2780

  Fragments arranged by size: 

                2780    1030     317     302     195     125      68      15
            
 
BbeI G_GCGC'C

Cuts at:   1788    2266    2514    1788 
   Size:        478     248    4106

  Fragments arranged by size: 

                4106     478     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    279     279 
   Size:       4832
 
BbvCI CC'TCA_GC

Cuts at:    485    1064     485 
   Size:        579    4253
 
BciVI GTATCCnnnnn_n'

Cuts at:    809    2730    3491    4832     809 
   Size:       1921     761    1341     809

  Fragments arranged by size: 

                1921    1341     809     761
 
BclI T'GATC_A

Cuts at:    674    2352     674 
   Size:       1678    3154
 
BglI GCCn_nnn'nGGC

Cuts at:    160    1309    1572     160 
   Size:       1149     263    3420

  Fragments arranged by size: 

                3420    1149     263
 
BglII A'GATC_T

Cuts at:   2347    4384    2347 
   Size:       2037    2795
 
BlpI GC'TnA_GC

Cuts at:   1944    1944 
   Size:       4832
 
Bme1580I G_kGCm'C

Cuts at:    686    1141    1400    1568    1735    2457    2550    3606     686 
   Size:        455     259     168     167     722      93    1056    1912

  Fragments arranged by size: 

                1912    1056     722     455     259     168     167      93
            
 
BmrI ACTGGGnnnn_n'

Cuts at:   1214    2044    2125    2460    4604    1214 
   Size:        830      81     335    2144    1442

  Fragments arranged by size: 

                2144    1442     830     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3199    3199 
   Size:       4832
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    553     808    1091    1465    1489    1697    1822     553 
   Size:        255     283     374      24     208     125    3563

  Fragments arranged by size: 

                3563     374     283     255     208     125      24
 
Bpu10I CC'TnA_GC

Cuts at:    485    1064    1170     485 
   Size:        579     106    4147

  Fragments arranged by size: 

                4147     579     106
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2008    2887    3379    3677    2008 
   Size:        879     492     298    3163

  Fragments arranged by size: 

                3163     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4536    4536 
   Size:       4832
 
BsaAI yAC'GTr

Cuts at:   1824    2185    1824 
   Size:        361    4471
 
BsaBI GATnn'nnATC

Cuts at:      6    2346    4383       6 
   Size:       2340    2037     455

  Fragments arranged by size: 

                2340    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1514    1785    2263    2511    3213    4709    1514 
   Size:        271     478     248     702    1496    1637

  Fragments arranged by size: 

                1637    1496     702     478     271     248
 
BsaWI w'CCGG_w

Cuts at:   2021    2542    3220    3494    3641    4052    2021 
   Size:        521     678     274     147     411    2801

  Fragments arranged by size: 

                2801     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1166    1196    1166 
   Size:         30    4802
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    832    1178    1331    1444    1615    1623    1753     832 
   Size:        346     153     113     171       8     130    3911

  Fragments arranged by size: 

                3911     346     171     153     130     113       8
 
BseYI C'CCAG_C

Cuts at:    247    1165    1414    2257    3015    3592    4088     247 
   Size:        918     249     843     758     577     496     991

  Fragments arranged by size: 

                 991     918     843     758     577     496     249
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    538    1223     538 
   Size:        685    4147
 
BsiEI CG_ry'CG

Cuts at:    104    2374    2420    3628     104 
   Size:       2270      46    1208    1308

  Fragments arranged by size: 

                2270    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    353     530     686     824    1443    1778    1846    2624    2814 
   Size:        177     156     138     619     335      68     778     190

Cuts at:   2814    3606     353 
   Size:        792    1579

  Fragments arranged by size: 

                1579     792     778     619     335     190     177     156
                 138      68
 
Bsp1286I G_dGCh'C

Cuts at:    353     530     686     824    1141    1400    1443    1568    1735 
   Size:        177     156     138     317     259      43     125     167

Cuts at:   1735    1763    1778    1846    2457    2550    2624    2814    2876 
   Size:         28      15      68     611      93      74     190      62

Cuts at:   2876    3606     353 
   Size:        730    1579

  Fragments arranged by size: 

                1579     730     611     317     259     190     177     167
                 156     138     125      93      74      68      62      43
                  28      15
 
BspEI T'CCGG_A

Cuts at:   4052    4052 
   Size:       4832
 
BspHI T'CATG_A

Cuts at:    662     671    4008     662 
   Size:          9    3337    1486

  Fragments arranged by size: 

                3337    1486       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    955     964    1882    2096    2398    2779     955 
   Size:          9     918     214     302     381    3008

  Fragments arranged by size: 

                3008     918     381     302     214       9
 
BsrBI CCG'CTC

Cuts at:     50    1458    3124    3178    4662    4827      50 
   Size:       1408    1666      54    1484     165      55

  Fragments arranged by size: 

                1666    1484    1408     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    836    2744     836 
   Size:       1908    2924
 
BsrFI r'CCGG_y

Cuts at:   1781    2830    3011    3220    1781 
   Size:       1049     181     209    3393

  Fragments arranged by size: 

                3393    1049     209     181
 
BsrGI T'GTAC_A

Cuts at:    263     602     650     263 
   Size:        339      48    4445

  Fragments arranged by size: 

                4445     339      48
 
BssSI C'ACGA_G

Cuts at:   3103    3461    3103 
   Size:        358    4474
 
BstAPI GCAn_nnn'nTGC

Cuts at:    851    1488    2209     851 
   Size:        637     721    3474

  Fragments arranged by size: 

                3474     721     637
 
BstBI TT'CG_AA

Cuts at:    110    3193     110 
   Size:       3083    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:    720     720 
   Size:       4832
 
BstYI r'GATC_y

Cuts at:      1    1857    2324    2347    2682    2928    3940    4026    4038 
   Size:       1856     467      23     335     246    1012      86      12

Cuts at:   4038    4384       1 
   Size:        346     449

  Fragments arranged by size: 

                1856    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1748    1748 
   Size:       4832
 
BtgI C'CryG_G

Cuts at:    560    1133     560 
   Size:        573    4259
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    965    2782    2963    3126     965 
   Size:       1817     181     163    2671

  Fragments arranged by size: 

                2671    1817     181     163
 
BtsI GCAGTG_nn'

Cuts at:    146    1504    2075     146 
   Size:       1358     571    2903

  Fragments arranged by size: 

                2903    1358     571
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1040    1075    1599    1634    1040 
   Size:         35     524      35    4238

  Fragments arranged by size: 

                4238     524      35      35
 
DraI TTT'AAA

Cuts at:   1831    1831 
   Size:       4832
 
DraIII CAC_nnn'GTG

Cuts at:    561     561 
   Size:       4832
 
DrdI GACnn_nn'nnGTC

Cuts at:   2538    3396    2538 
   Size:        858    3974
 
EaeI y'GGCC_r

Cuts at:    152     722    2417    2591    2982    3009    4354     152 
   Size:        570    1695     174     391      27    1345     630

  Fragments arranged by size: 

                1695    1345     630     570     391     174      27
 
EagI C'GGCC_G

Cuts at:   2417    2417 
   Size:       4832
 
EarI CTCTTCn'nnn_

Cuts at:    448     455     463     854    2855    3065     448 
   Size:          7       8     391    2001     210    2215

  Fragments arranged by size: 

                2215    2001     391     210       8       7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1768    3040    3350    3496    4642    1768 
   Size:       1272     310     146    1146    1958

  Fragments arranged by size: 

                1958    1272    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    822    1441    1776    1844     822 
   Size:        619     335      68    3810

  Fragments arranged by size: 

                3810     619     335      68
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    405     553     808    1091    1465    1489    1568    1697    1822 
   Size:        148     255     283     374      24      79     129     125

Cuts at:   1822    2657    3089    3836    4104     405 
   Size:        835     432     747     268    1133

  Fragments arranged by size: 

                1133     835     747     432     374     283     268     255
                 148     129     125      79      24
 
EcoO109I rG'GnC_Cy

Cuts at:   1086    1138    1564    1565    1971    1086 
   Size:         52     426       1     406    3947

  Fragments arranged by size: 

                3947     426     406      52       1
 
EcoRI G'AATT_C

Cuts at:   1836    1836 
   Size:       4832
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    336    2851     336 
   Size:       2515    2317
 
FspI TGC'GCA

Cuts at:   2613    2613 
   Size:       4832
 
HaeII r_GCGC'y

Cuts at:    166    1788    2266    2514    3536    4515     166 
   Size:       1622     478     248    1022     979     483

  Fragments arranged by size: 

                1622    1022     979     483     478     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2674    2704    2706    2736    2674 
   Size:         30       2      30    4770

  Fragments arranged by size: 

                4770      30      30       2
 
HincII GTy'rAC

Cuts at:   1590    1871    1590 
   Size:        281    4551
 
KasI G'GCGC_C

Cuts at:   1784    2262    2510    1784 
   Size:        478     248    4106

  Fragments arranged by size: 

                4106     478     248
 
KpnI G_GTAC'C

Cuts at:   1852    1852 
   Size:       4832
 
MluI A'CGCG_T

Cuts at:   4265    4265 
   Size:       4832
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3503    3687    3503 
   Size:        184    4648
 
MscI TGG'CCA

Cuts at:    724    2593     724 
   Size:       1869    2963
 
MslI CAynn'nnrTG

Cuts at:    139     223     514    2821    2948     139 
   Size:         84     291    2307     127    2023

  Fragments arranged by size: 

                2307    2023     291     127      84
 
MspA1I CmG'CkG

Cuts at:    914     936    1243    1943    2257    2617    3630    3875     914 
   Size:         22     307     700     314     360    1013     245    1871

  Fragments arranged by size: 

                1871    1013     700     360     314     307     245      22
            
 
NaeI GCC'GGC

Cuts at:   1783    3013    1783 
   Size:       1230    3602
 
NarI GG'CG_CC

Cuts at:   1785    2263    2511    1785 
   Size:        478     248    4106

  Fragments arranged by size: 

                4106     478     248
 
NcoI C'CATG_G

Cuts at:   1133    1133 
   Size:       4832
 
NgoMIV G'CCGG_C

Cuts at:   1781    3011    1781 
   Size:       1230    3602
 
NspI r_CATG'y

Cuts at:    609    1116    1885    2385    3292     609 
   Size:        507     769     500     907    2149

  Fragments arranged by size: 

                2149     907     769     507     500
 
PasI CC'CwG_GG

Cuts at:    584    1083     584 
   Size:        499    4333
 
PciI A'CATG_T

Cuts at:   3288    3288 
   Size:       4832
 
PflMI CCAn_nnn'nTGG

Cuts at:    480     831    1294    1312     480 
   Size:        351     463      18    4000

  Fragments arranged by size: 

                4000     463     351      18
 
PmeI GTTT'AAAC

Cuts at:   1831    1831 
   Size:       4832
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3971    4003    3971 
   Size:         32    4800
 
PspOMI G'GGCC_C

Cuts at:   1137    1564    1137 
   Size:        427    4405
 
PstI C_TGCA'G

Cuts at:   1484    1529    1796    1879    2564    1484 
   Size:         45     267      83     685    3752

  Fragments arranged by size: 

                3752     685     267      83      45
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4832
 
PvuII CAG'CTG

Cuts at:    914    1243    2257    2617     914 
   Size:        329    1014     360    3129

  Fragments arranged by size: 

                3129    1014     360     329
 
RsrII CG'GwC_CG

Cuts at:   3027    3027 
   Size:       4832
 
SacI G_AGCT'C

Cuts at:    824    1443    1778    1846     824 
   Size:        619     335      68    3810

  Fragments arranged by size: 

                3810     619     335      68
 
SalI G'TCGA_C

Cuts at:   1869    1869 
   Size:       4832
 
SapI GCTCTTCn'nnn_

Cuts at:   2855    3065    2855 
   Size:        210    4622
 
SbfI CC_TGCA'GG

Cuts at:   1879    1879 
   Size:       4832
 
ScaI AGT'ACT

Cuts at:   4349    4349 
   Size:       4832
 
SfcI C'TryA_G

Cuts at:     33    1480    1525    1613    1792    1875    2560    3553    3744 
   Size:       1447      45      88     179      83     685     993     191

Cuts at:   3744      33 
   Size:       1121

  Fragments arranged by size: 

                1447    1121     993     685     191     179      88      83
                  45
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1572    1572 
   Size:       4832
 
SfoI GGC'GCC

Cuts at:   1786    2264    2512    1786 
   Size:        478     248    4106

  Fragments arranged by size: 

                4106     478     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4832
 
SgrAI Cr'CCGG_yG

Cuts at:   1781    1781 
   Size:       4832
 
SmaI CCC'GGG

Cuts at:   1854    1854 
   Size:       4832
 
SmlI C'TyrA_G

Cuts at:   1987    2902    3394    3656    1987 
   Size:        915     492     262    3163

  Fragments arranged by size: 

                3163     915     492     262
 
SnaBI TAC'GTA

Cuts at:   1824    1824 
   Size:       4832
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4832
 
SphI G_CATG'C

Cuts at:   1885    2385    1885 
   Size:        500    4332
 
StuI AGG'CCT

Cuts at:    274    1710     274 
   Size:       1436    3396
 
StyI C'CwwG_G

Cuts at:   1133    1559    1966    2320    1133 
   Size:        426     407     354    3645

  Fragments arranged by size: 

                3645     426     407     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2415    2548    3007    3216    2415 
   Size:        133     459     209    4031

  Fragments arranged by size: 

                4031     459     209     133
 
TatI w'GTAC_w

Cuts at:    263     602     650    4347     263 
   Size:        339      48    3697     748

  Fragments arranged by size: 

                3697     748     339      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    783     815    1166    1198    4349    4381     783 
   Size:         32     351      32    3151      32    1234

  Fragments arranged by size: 

                3151    1234     351      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    237     838    2629     237 
   Size:        601    1791    2440

  Fragments arranged by size: 

                2440    1791     601
 
XbaI T'CTAG_A

Cuts at:   1863    1863 
   Size:       4832
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    249     960    2327     249 
   Size:        711    1367    2754

  Fragments arranged by size: 

                2754    1367     711
 
XmaI C'CCGG_G

Cuts at:   1852    1852 
   Size:       4832
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI    PflMI     PmeI     PpiI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     AscI    AvrII
     BaeI     BcgI    BfrBI    BmgBI     BmtI    BsiWI     BsmI    BsmBI
   BssHII   BstEII  BstZ17I     ClaI    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PfoI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI
    SacII    SanDI    SexAI     SrfI     SspI     SwaI     XhoI     XmnI
     ZraI
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