Restriction map for pF1KSDA1810
 (Linear) (Six-base) MAPSORT of: KIAA1810.seq  Check: 9615  from: 1  to: 1695



KIAA1810.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     838    1695 
   Size:        838     857
 
AclI AA'CG_TT

Cuts at:      0     237    1695 
   Size:        237    1458
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279    1442    1695 
   Size:        279    1163     253

  Fragments arranged by size: 

                1163     279     253
 
AleI CACnn'nnGTG

Cuts at:      0      13      97    1695 
   Size:         13      84    1598

  Fragments arranged by size: 

                1598      84      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1120    1156    1168    1186    1341    1362    1570    1594 
   Size:       1120      36      12      18     155      21     208      24

Cuts at:   1594    1674    1695 
   Size:         80      21

  Fragments arranged by size: 

                1120     208     155      80      36      24      21      21
                  18      12
 
ApaI G_GGCC'C

Cuts at:      0    1015    1442    1695 
   Size:       1015     427     253

  Fragments arranged by size: 

                1015     427     253
 
ApaLI G'TGCA_C

Cuts at:      0     556    1695 
   Size:        556    1139
 
BanI G'GyrC_C

Cuts at:      0     748    1123    1658    1695 
   Size:        748     375     535      37

  Fragments arranged by size: 

                 748     535     375      37
 
BanII G_rGCy'C

Cuts at:      0     698    1015    1317    1442    1637    1652    1695 
   Size:        698     317     302     125     195      15      43

  Fragments arranged by size: 

                 698     317     302     195     125      43      15
 
BbeI G_GCGC'C

Cuts at:      0    1662    1695 
   Size:       1662      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     153    1695 
   Size:        153    1542
 
BbvCI CC'TCA_GC

Cuts at:      0     359     938    1695 
   Size:        359     579     757

  Fragments arranged by size: 

                 757     579     359
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     683    1695 
   Size:        683    1012
 
BclI T'GATC_A

Cuts at:      0     548    1695 
   Size:        548    1147
 
BglI GCCn_nnn'nGGC

Cuts at:      0      34    1183    1446    1695 
   Size:         34    1149     263     249

  Fragments arranged by size: 

                1149     263     249      34
 
Bme1580I G_kGCm'C

Cuts at:      0     560    1015    1274    1442    1609    1695 
   Size:        560     455     259     168     167      86

  Fragments arranged by size: 

                 560     455     259     168     167      86
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1088    1695 
   Size:       1088     607
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     682     965    1339    1363    1571    1695 
   Size:        427     255     283     374      24     208     124

  Fragments arranged by size: 

                 427     374     283     255     208     124      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     359     938    1044    1695 
   Size:        359     579     106     651

  Fragments arranged by size: 

                 651     579     359     106
 
BsaHI Gr'CG_yC

Cuts at:      0    1388    1659    1695 
   Size:       1388     271      36

  Fragments arranged by size: 

                1388     271      36
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1040    1070    1695 
   Size:       1040      30     625

  Fragments arranged by size: 

                1040     625      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     706    1052    1205    1318    1489    1497    1627    1695 
   Size:        706     346     153     113     171       8     130      68

  Fragments arranged by size: 

                 706     346     171     153     130     113      68       8
            
 
BseYI C'CCAG_C

Cuts at:      0     121    1039    1288    1695 
   Size:        121     918     249     407

  Fragments arranged by size: 

                 918     407     249     121
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    1097    1695 
   Size:        412     685     598

  Fragments arranged by size: 

                 685     598     412
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     404     560     698    1317    1652    1695 
   Size:        227     177     156     138     619     335      43

  Fragments arranged by size: 

                 619     335     227     177     156     138      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     404     560     698    1015    1274    1317    1442 
   Size:        227     177     156     138     317     259      43     125

Cuts at:   1442    1609    1637    1652    1695 
   Size:        167      28      15      43

  Fragments arranged by size: 

                 317     259     227     177     167     156     138     125
                  43      43      28      15
 
BspHI T'CATG_A

Cuts at:      0     536     545    1695 
   Size:        536       9    1150

  Fragments arranged by size: 

                1150     536       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     829     838    1695 
   Size:        829       9     857

  Fragments arranged by size: 

                 857     829       9
 
BsrBI CCG'CTC

Cuts at:      0    1332    1695 
   Size:       1332     363
 
BsrDI GCAATG_nn'

Cuts at:      0     710    1695 
   Size:        710     985
 
BsrFI r'CCGG_y

Cuts at:      0    1655    1695 
   Size:       1655      40
 
BsrGI T'GTAC_A

Cuts at:      0     137     476     524    1695 
   Size:        137     339      48    1171

  Fragments arranged by size: 

                1171     339     137      48
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     725    1362    1695 
   Size:        725     637     333

  Fragments arranged by size: 

                 725     637     333
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     594    1695 
   Size:        594    1101
 
Bsu36I CC'TnA_GG

Cuts at:      0    1622    1695 
   Size:       1622      73
 
BtgI C'CryG_G

Cuts at:      0     434    1007    1695 
   Size:        434     573     688

  Fragments arranged by size: 

                 688     573     434
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     839    1695 
   Size:        839     856
 
BtsI GCAGTG_nn'

Cuts at:      0      20    1378    1695 
   Size:         20    1358     317

  Fragments arranged by size: 

                1358     317      20
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     914     949    1473    1508    1695 
   Size:        914      35     524      35     187

  Fragments arranged by size: 

                 914     524     187      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     435    1695 
   Size:        435    1260
 
EaeI y'GGCC_r

Cuts at:      0      26     596    1695 
   Size:         26     570    1099

  Fragments arranged by size: 

                1099     570      26
 
EarI CTCTTCn'nnn_

Cuts at:      0     322     329     337     728    1695 
   Size:        322       7       8     391     967

  Fragments arranged by size: 

                 967     391     322       8       7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1642    1695 
   Size:       1642      53
 
EcoICRI GAG'CTC

Cuts at:      0     696    1315    1650    1695 
   Size:        696     619     335      45

  Fragments arranged by size: 

                 696     619     335      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     427     682     965    1339    1363    1442    1571 
   Size:        279     148     255     283     374      24      79     129

Cuts at:   1571    1695 
   Size:        124

  Fragments arranged by size: 

                 374     283     279     255     148     129     124      79
                  24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     960    1012    1438    1439    1695 
   Size:        960      52     426       1     256

  Fragments arranged by size: 

                 960     426     256      52       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     210    1695 
   Size:        210    1485
 
HaeII r_GCGC'y

Cuts at:      0      40    1662    1695 
   Size:         40    1622      33

  Fragments arranged by size: 

                1622      40      33
 
HincII GTy'rAC

Cuts at:      0    1464    1695 
   Size:       1464     231
 
KasI G'GCGC_C

Cuts at:      0    1658    1695 
   Size:       1658      37
 
MscI TGG'CCA

Cuts at:      0     598    1695 
   Size:        598    1097
 
MslI CAynn'nnrTG

Cuts at:      0      13      97     388    1695 
   Size:         13      84     291    1307

  Fragments arranged by size: 

                1307     291      84      13
 
MspA1I CmG'CkG

Cuts at:      0     788     810    1117    1695 
   Size:        788      22     307     578

  Fragments arranged by size: 

                 788     578     307      22
 
NaeI GCC'GGC

Cuts at:      0    1657    1695 
   Size:       1657      38
 
NarI GG'CG_CC

Cuts at:      0    1659    1695 
   Size:       1659      36
 
NcoI C'CATG_G

Cuts at:      0    1007    1695 
   Size:       1007     688
 
NgoMIV G'CCGG_C

Cuts at:      0    1655    1695 
   Size:       1655      40
 
NspI r_CATG'y

Cuts at:      0     483     990    1695 
   Size:        483     507     705

  Fragments arranged by size: 

                 705     507     483
 
PasI CC'CwG_GG

Cuts at:      0     458     957    1695 
   Size:        458     499     738

  Fragments arranged by size: 

                 738     499     458
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     354     705    1168    1186    1695 
   Size:        354     351     463      18     509

  Fragments arranged by size: 

                 509     463     354     351      18
 
PspOMI G'GGCC_C

Cuts at:      0    1011    1438    1695 
   Size:       1011     427     257

  Fragments arranged by size: 

                1011     427     257
 
PstI C_TGCA'G

Cuts at:      0    1358    1403    1670    1695 
   Size:       1358      45     267      25

  Fragments arranged by size: 

                1358     267      45      25
 
PvuII CAG'CTG

Cuts at:      0     788    1117    1695 
   Size:        788     329     578

  Fragments arranged by size: 

                 788     578     329
 
SacI G_AGCT'C

Cuts at:      0     698    1317    1652    1695 
   Size:        698     619     335      43

  Fragments arranged by size: 

                 698     619     335      43
 
SfcI C'TryA_G

Cuts at:      0    1354    1399    1487    1666    1695 
   Size:       1354      45      88     179      29

  Fragments arranged by size: 

                1354     179      88      45      29
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1446    1695 
   Size:       1446     249
 
SfoI GGC'GCC

Cuts at:      0    1660    1695 
   Size:       1660      35
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1655    1695 
   Size:       1655      40
 
StuI AGG'CCT

Cuts at:      0     148    1584    1695 
   Size:        148    1436     111

  Fragments arranged by size: 

                1436     148     111
 
StyI C'CwwG_G

Cuts at:      0    1007    1433    1695 
   Size:       1007     426     262

  Fragments arranged by size: 

                1007     426     262
 
TatI w'GTAC_w

Cuts at:      0     137     476     524    1695 
   Size:        137     339      48    1171

  Fragments arranged by size: 

                1171     339     137      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     657     689    1040    1072    1695 
   Size:        657      32     351      32     623

  Fragments arranged by size: 

                 657     623     351      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     111     712    1695 
   Size:        111     601     983

  Fragments arranged by size: 

                 983     601     111
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     123     834    1695 
   Size:        123     711     861

  Fragments arranged by size: 

                 861     711     123
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AleI    AlwNI     ApaI    ApaLI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI Bme1580I
     BmrI     BpmI   Bpu10I    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BstAPI    BstXI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII
   HincII     KasI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI    PflMI   PspOMI     PstI    PvuII     SacI
     SfcI     SfiI     SfoI    SgrAI     StuI     StyI     TatI     TstI
  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BcgI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI   BssHII    BssSI    BstBI   BstEII    BstYI
  BstZ17I     ClaI     DraI     DrdI     EagI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     XbaI     XhoI     XmaI
     XmnI     ZraI
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