Restriction map for pF1KSDA1665
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
AlwNI       AocI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvaI        AviII       AvrII       BanI        BbeI        
BbrPI       BbsI        BbvCI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsgI        
BsiEI       BsiWI       BsmI        BsoBI       BspDI       
BspHI       BspLU11I    BsrBI       BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MseI        MspA1I      
MunI        MvnI        MwoI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tru9I       Tsp509I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII         32  TCCGGA               T^CCGGA
AflIII        476  ACACGT               A^C[GA][CT]GT
Alw44I        289  GTGCAC               G^TGCAC
ApaI          490  GGGCCC               GGGCC^C
ApaLI         289  GTGCAC               G^TGCAC
AspI           25  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         602  GGACC                G^G[AT]CC
BalI          101  TGGCCA               TGG^CCA
BanII         490  GGGCCC               G[GA]GC[CT]^C
BcgI           69  CGACTCTATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          450  CCGGG                CC^[GC]GG
BlpI          497  GCTCAGC              GC^T[ACGT]AGC
BpmI          160  CTGGAG               CTGGAG(16/14)
Bpu1102I      497  GCTCAGC              GC^T[ACGT]AGC
BsaWI          32  TCCGGA               [AT]^CCGG[AT]
BseAI          32  TCCGGA               T^CCGGA
Bsp1407I      116  TGTACA               T^GTACA
BspEI          32  TCCGGA               T^CCGGA
BspMI         372  GCAGGT               ACCTGC(4/8)
BsrFI         430  ACCGGC               [GA]^CCGG[CT]
BsrGI         116  TGTACA               T^GTACA
Cac8I         348  GCCAGC               GC[ACGT]^[ACGT]GC
CelII         497  GCTCAGC              GC^T[ACGT]AGC
CfoI          191  GCGC                 GCG^C
Cfr10I        430  ACCGGC               [GA]^CCGG[CT]
DdeI          498  CTCAG                C^T[ACGT]AG
DraII         489  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EaeI          101  TGGCCA               [CT]^GGCC[GA]
EcoO109I      489  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRV         148  GATATC               GAT^ATC
EcoT14I        43  CCTTGG               C^C[AT][AT]GG
HhaI          191  GCGC                 GCG^C
HinP1I        191  GCGC                 G^CGC
HincII        472  GTTGAC               GT[CT]^[GA]AC
HindII        472  GTTGAC               GT[CT]^[GA]AC
HphI          152  TCACC                GGTGA(8/7)
MaeIII        336  GTCAC                ^GT[ACGT]AC
MluNI         101  TGGCCA               TGG^CCA
MroI           32  TCCGGA               T^CCGGA
MscI          101  TGGCCA               TGG^CCA
MslI          552  CACGCTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          450  CCGGG                CC^[GC]GG
NlaIII        423  CATG                 CATG^
PflFI          25  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SgrAI         429  CACCGGCG             C[GA]^CCGG[CT]G
SspBI         116  TGTACA               T^GTACA
StuI          579  AGGCCT               AGG^CCT
StyI           43  CCTTGG               C^C[AT][AT]GG
TfiI          249  GATTC                G^A[AT]TC
Tsp45I        336  GTCAC                ^GT[GC]AC
Tth111I        25  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI          444  CCGC                 CCGC(-3/-1)
              367  GCGG                
AspHI         289  GTGCAC               G[AT]GC[AT]^C
              407  GAGCAC              
BamHI          35  GGATCC               G^GATCC
              562  GGATCC              
BfaI          241  CTAG                 C^TAG
              301  CTAG                
BsiHKAI       289  GTGCAC               G[AT]GC[AT]^C
              407  GAGCAC              
BsmAI         394  GAGAC                GTCTC(1/5)
              295  GTCTC               
BsmBI         294  CGTCTC               CGTCTC(1/5)
              394  GAGACG              
BsmFI         126  GGGAC                GGGAC(10/14)
              480  GTCCC               
BsrI          159  ACTGG                ACTGG(1/-1)
              518  CCAGT               
MaeI          241  CTAG                 C^TAG
              301  CTAG                
MboII         261  GAAGA                GAAGA(8/7)
              308  TCTTC               
PstI          273  CTGCAG               CTGCA^G
              340  CTGCAG              
SfcI          273  CTGCAG               C^T[GA][CT]AG
              340  CTGCAG              
TaqI          311  TCGA                 T^CGA
              359  TCGA                
TthHB8I       311  TCGA                 T^CGA
              359  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AfaI        AluI        AlwI        BbvI        BmyI        
BsaJI       BseRI       BsiYI       BslI        Bsp1286I    
BssKI       BstF5I      BstNI       BstYI       Cfr13I      
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       ItaI        MaeII       
MboI        MflI        MnlI        MspI        MvaI        
NdeII       NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TseI        TspRI       
XhoII       
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