Restriction map for pF1KSDA1612
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BbrPI       BcgI        BciVI       BclI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaI        BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BsrBI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoT22I     FauI        FbaI        FseI        
FspI        HincII      HindII      HpaI        KspI        
MfeI        MflI        MluI        MroI        MunI        
MvnI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        TaqI        Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        538  GGTACC               G^GTACC
AccI         1507  GTCTAC               GT^[AC][GT]AC
AflII         895  CTTAAG               C^TTAAG
AgeI         1330  ACCGGT               A^CCGGT
Asp718I       538  GGTACC               G^GTACC
AvaI           35  CCCGGG               C^[CT]CG[GA]G
BalI         1211  TGGCCA               TGG^CCA
BbeI          482  GGCGCC               GGCGC^C
BbsI         1326  GAAGAC               GAAGAC(2/6)
BfrI          895  CTTAAG               C^TTAAG
BpmI          126  CTGGAG               CTGGAG(16/14)
BpuAI        1326  GAAGAC               GAAGAC(2/6)
BsaBI        1209  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         997  GAGGAG               GAGGAG(10/8)
BsoBI          35  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      551  TGTACA               T^GTACA
BspMI         111  GCAGGT               ACCTGC(4/8)
BsrFI        1330  ACCGGT               [GA]^CCGG[CT]
BsrGI         551  TGTACA               T^GTACA
BsrI          618  ACTGG                ACTGG(1/-1)
Cac8I          15  GCCAGC               GC[ACGT]^[ACGT]GC
CfoI          483  GCGC                 GCG^C
Cfr10I       1330  ACCGGT               [GA]^CCGG[CT]
DraII         963  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoNI         518  CCTGGGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      963  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         439  GAATTC               G^AATTC
EcoRV        1156  GATATC               GAT^ATC
HaeII         482  GGCGCC               [GA]GCGC^[CT]
HhaI          483  GCGC                 GCG^C
HinP1I        483  GCGC                 G^CGC
KasI          482  GGCGCC               G^GCGCC
KpnI          538  GGTACC               GGTAC^C
MaeII         687  ACGT                 A^CGT
MamI         1209  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1211  TGGCCA               TGG^CCA
MscI         1211  TGGCCA               TGG^CCA
MspA1I         17  CAGCGG               C[AC]G^C[GT]G
NarI          482  GGCGCC               GG^CGCC
PflMI        1417  CCAGAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1330  ACCGGT               A^CCGGT
PpuMI         963  AGGTCCC              [GA]G^G[AT]CC[CT]
SapI         1462  GAAGAGC              GCTCTTC(1/4)
SfaNI         448  GATGC                GCATC(5/9)
SfcI         1509  CTACAG               C^T[GA][CT]AG
SfoI          482  GGCGCC               GGC^GCC
SmaI           35  CCCGGG               CCC^GGG
SmlI          895  CTTAAG               C^T[CT][GA]AG
SspBI         551  TGTACA               T^GTACA
Van91I       1417  CCAGAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          35  CCCGGG               C^CCGGG
XmaI           35  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI          138  GACGCC               G[GA]^CG[CT]C
              482  GGCGCC              
AflIII        419  ACATGT               A^C[GA][CT]GT
              942  ACATGT              
AlwI          195  GATCC                GGATC(4/5)
              806  GGATC               
BanI          482  GGCGCC               G^G[CT][GA]CC
              538  GGTACC              
BanII          22  GAGCCC               G[GA]GC[CT]^C
              765  GAGCCC              
BbvCI         118  GCTGAGG              CCTCAGC(-5/-2)
              994  GCTGAGG             
BfaI         1175  CTAG                 C^TAG
             1378  CTAG                
BmyI           22  GAGCCC               G[AGT]GC[ACT]^C
              765  GAGCCC              
BsaHI         138  GACGCC               G[GA]^CG[CT]C
              482  GGCGCC              
BsaWI         368  TCCGGT               [AT]^CCGG[AT]
             1330  ACCGGT              
Bsp1286I       22  GAGCCC               G[AGT]GC[ACT]^C
              765  GAGCCC              
BspHI         786  TCATGA               T^CATGA
             1350  TCATGA              
BspLU11I      419  ACATGT               A^CATGT
              942  ACATGT              
BsrDI        1443  CATTGC               GCAATG(2/0)
             1238  GCAATG              
DraIII        187  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              652  CACCAGGTG           
EaeI           13  CGGCCA               [CT]^GGCC[GA]
             1211  TGGCCA              
EcoT14I       929  CCTTGG               C^C[AT][AT]GG
             1305  CCAAGG              
HgaI           82  GACGC                GACGC(5/10)
              138  GACGC               
Hin1I         138  GACGCC               G[GA]^CG[CT]C
              482  GGCGCC              
HindIII      1006  AAGCTT               A^AGCTT
             1090  AAGCTT              
MaeI         1175  CTAG                 C^TAG
             1378  CTAG                
NspI          419  ACATGT               [GA]CATG^[CT]
              942  ACATGT              
PleI          726  GACTC                GAGTC(4/5)
             1524  GACTC               
RcaI          786  TCATGA               T^CATGA
             1350  TCATGA              
SexAI         506  ACCTGGT              A^CC[AT]GGT
              653  ACCAGGT             
StyI          929  CCTTGG               C^C[AT][AT]GG
             1305  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
Asp700I     AvaII       BbvI        BcnI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       StuI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XmnI        
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