Restriction map for pF1KSDA1612
 (Linear) (Six-base) MAPSORT of: KIAA1612.seq  Check: 390  from: 1  to: 1626



KIAA1612.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     102    1626 
   Size:        102    1524
 
AccI GT'mk_AC

Cuts at:      0    1508    1626 
   Size:       1508     118
 
Acc65I G'GTAC_C

Cuts at:      0     538    1626 
   Size:        538    1088
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     683     695     904    1108    1184    1626 
   Size:        220     463      12     209     204      76     442

  Fragments arranged by size: 

                 463     442     220     209     204      76      12
 
AflII C'TTAA_G

Cuts at:      0     895    1626 
   Size:        895     731
 
AflIII A'CryG_T

Cuts at:      0     419     942    1626 
   Size:        419     523     684

  Fragments arranged by size: 

                 684     523     419
 
AgeI A'CCGG_T

Cuts at:      0    1330    1626 
   Size:       1330     296
 
ApoI r'AATT_y

Cuts at:      0     350     439     845     875     907    1369    1531    1626 
   Size:        350      89     406      30      32     462     162      95

  Fragments arranged by size: 

                 462     406     350     162      95      89      32      30
            
 
AvaI C'yCGr_G

Cuts at:      0      35    1626 
   Size:         35    1591
 
BanI G'GyrC_C

Cuts at:      0     482     538    1626 
   Size:        482      56    1088

  Fragments arranged by size: 

                1088     482      56
 
BanII G_rGCy'C

Cuts at:      0      26     769    1626 
   Size:         26     743     857

  Fragments arranged by size: 

                 857     743      26
 
BbeI G_GCGC'C

Cuts at:      0     486    1626 
   Size:        486    1140
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1333    1626 
   Size:       1333     293
 
BbvCI CC'TCA_GC

Cuts at:      0     119     995    1626 
   Size:        119     876     631

  Fragments arranged by size: 

                 876     631     119
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     147    1626 
   Size:        147    1479
 
Bpu10I CC'TnA_GC

Cuts at:      0     119     995    1190    1626 
   Size:        119     876     195     436

  Fragments arranged by size: 

                 876     436     195     119
 
BsaBI GATnn'nnATC

Cuts at:      0    1213    1626 
   Size:       1213     413
 
BsaHI Gr'CG_yC

Cuts at:      0     139     483    1626 
   Size:        139     344    1143

  Fragments arranged by size: 

                1143     344     139
 
BsaWI w'CCGG_w

Cuts at:      0     368    1330    1626 
   Size:        368     962     296

  Fragments arranged by size: 

                 962     368     296
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     519     549     717     747    1626 
   Size:        519      30     168      30     879

  Fragments arranged by size: 

                 879     519     168      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1012    1626 
   Size:       1012     614
 
BseYI C'CCAG_C

Cuts at:      0      30    1409    1626 
   Size:         30    1379     217

  Fragments arranged by size: 

                1379     217      30
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     769    1626 
   Size:         26     743     857

  Fragments arranged by size: 

                 857     743      26
 
BspHI T'CATG_A

Cuts at:      0     786    1350    1626 
   Size:        786     564     276

  Fragments arranged by size: 

                 786     564     276
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     102    1626 
   Size:        102    1524
 
BsrDI GCAATG_nn'

Cuts at:      0    1245    1442    1626 
   Size:       1245     197     184

  Fragments arranged by size: 

                1245     197     184
 
BsrFI r'CCGG_y

Cuts at:      0    1330    1626 
   Size:       1330     296
 
BsrGI T'GTAC_A

Cuts at:      0     551    1626 
   Size:        551    1075
 
DraIII CAC_nnn'GTG

Cuts at:      0     192     657    1626 
   Size:        192     465     969

  Fragments arranged by size: 

                 969     465     192
 
EaeI y'GGCC_r

Cuts at:      0      13    1211    1626 
   Size:         13    1198     415

  Fragments arranged by size: 

                1198     415      13
 
EarI CTCTTCn'nnn_

Cuts at:      0     310     315     848    1457    1626 
   Size:        310       5     533     609     169

  Fragments arranged by size: 

                 609     533     310     169       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      85    1626 
   Size:         85    1541
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     147     220     683     695     904    1108    1184    1626 
   Size:        147      73     463      12     209     204      76     442

  Fragments arranged by size: 

                 463     442     209     204     147      76      73      12
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     522    1626 
   Size:        522    1104
 
EcoO109I rG'GnC_Cy

Cuts at:      0     964    1626 
   Size:        964     662
 
EcoRI G'AATT_C

Cuts at:      0     439    1626 
   Size:        439    1187
 
EcoRV GAT'ATC

Cuts at:      0    1158    1626 
   Size:       1158     468
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1511    1593    1626 
   Size:       1511      82      33

  Fragments arranged by size: 

                1511      82      33
 
HaeII r_GCGC'y

Cuts at:      0     486    1626 
   Size:        486    1140
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     355     387     391     423     717     749    1626 
   Size:        355      32       4      32     294      32     877

  Fragments arranged by size: 

                 877     355     294      32      32      32       4
 
HindIII A'AGCT_T

Cuts at:      0    1006    1090    1626 
   Size:       1006      84     536

  Fragments arranged by size: 

                1006     536      84
 
KasI G'GCGC_C

Cuts at:      0     482    1626 
   Size:        482    1144
 
KpnI G_GTAC'C

Cuts at:      0     542    1626 
   Size:        542    1084
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1097    1626 
   Size:       1097     529
 
MscI TGG'CCA

Cuts at:      0    1213    1626 
   Size:       1213     413
 
MslI CAynn'nnrTG

Cuts at:      0     163     169     418     467     941    1626 
   Size:        163       6     249      49     474     685

  Fragments arranged by size: 

                 685     474     249     163      49       6
 
MspA1I CmG'CkG

Cuts at:      0      19    1626 
   Size:         19    1607
 
NarI GG'CG_CC

Cuts at:      0     483    1626 
   Size:        483    1143
 
NspI r_CATG'y

Cuts at:      0     423     946    1626 
   Size:        423     523     680

  Fragments arranged by size: 

                 680     523     423
 
PasI CC'CwG_GG

Cuts at:      0     518    1626 
   Size:        518    1108
 
PciI A'CATG_T

Cuts at:      0     419     942    1626 
   Size:        419     523     684

  Fragments arranged by size: 

                 684     523     419
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1423    1626 
   Size:       1423     203
 
PpuMI rG'GwC_Cy

Cuts at:      0     964    1626 
   Size:        964     662
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     740     772    1626 
   Size:        740      32     854

  Fragments arranged by size: 

                 854     740      32
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1457    1626 
   Size:       1457     169
 
SexAI A'CCwGG_T

Cuts at:      0     506     653    1626 
   Size:        506     147     973

  Fragments arranged by size: 

                 973     506     147
 
SfcI C'TryA_G

Cuts at:      0    1509    1626 
   Size:       1509     117
 
SfoI GGC'GCC

Cuts at:      0     484    1626 
   Size:        484    1142
 
SmaI CCC'GGG

Cuts at:      0      37    1626 
   Size:         37    1589
 
SmlI C'TyrA_G

Cuts at:      0     895    1626 
   Size:        895     731
 
StuI AGG'CCT

Cuts at:      0     124     928    1085    1626 
   Size:        124     804     157     541

  Fragments arranged by size: 

                 804     541     157     124
 
StyI C'CwwG_G

Cuts at:      0     929    1305    1626 
   Size:        929     376     321

  Fragments arranged by size: 

                 929     376     321
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     523    1626 
   Size:        523    1103
 
TatI w'GTAC_w

Cuts at:      0     551    1391    1626 
   Size:        551     840     235

  Fragments arranged by size: 

                 840     551     235
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     519     551    1626 
   Size:        519      32    1075

  Fragments arranged by size: 

                1075     519      32
 
XmaI C'CCGG_G

Cuts at:      0      35    1626 
   Size:         35    1591
 
XmnI GAAnn'nnTTC

Cuts at:      0     233     569    1576    1626 
   Size:        233     336    1007      50

  Fragments arranged by size: 

                1007     336     233      50
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII   AflIII     AgeI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BpmI   Bpu10I
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   DraIII     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I
  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NarI
     NspI     PasI     PciI    PflMI    PpuMI     PsrI     SapI    SexAI
     SfcI     SfoI     SmaI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI     AhdI     AleI     AlfI     AloI    AlwNI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrBI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI
  EcoICRI     FseI     FspI    FspAI   HincII     HpaI     MfeI     MluI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI
     ZraI
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