Restriction map for pF1KSDA1580
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        Asp718I     AspEI       
AspI        AvaI        AviII       BbeI        BbrPI       
BbvCI       BclI        BfrI        BglI        BglII       
BsaAI       BsaBI       BsaHI       BsgI        BsiEI       
BsiWI       BsoBI       BspDI       BsrDI       BsrFI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       BstUI       Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RsrII       SacII       SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspI        
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII        210  TCCGGA               T^CCGGA
AflIII       1273  ACATGT               A^C[GA][CT]GT
AlwNI        1523  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           59  GGGCCC               GGGCC^C
AseI         1705  ATTAAT               AT^TAAT
AsnI         1705  ATTAAT               AT^TAAT
AvrII          46  CCTAGG               C^CTAGG
BalI         1601  TGGCCA               TGG^CCA
BamHI        1544  GGATCC               G^GATCC
BanI          571  GGTGCC               G^G[CT][GA]CC
BbsI         1569  GAAGAC               GAAGAC(2/6)
BcgI         1656  GCAGCACCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         884  GGATAC               GTATCC(6/5)
BcnI          983  CCCGG                CC^[GC]GG
BlnI           46  CCTAGG               C^CTAGG
BpmI         1436  CTCCAG               CTGGAG(16/14)
BpuAI        1569  GAAGAC               GAAGAC(2/6)
BsaI         1453  GAGACC               GGTCTC(1/5)
BseAI         210  TCCGGA               T^CCGGA
BseRI         995  CTCCTC               GAGGAG(10/8)
BsmBI        1391  CGTCTC               CGTCTC(1/5)
BsmI          780  GAATGC               GAATGC(1/-1)
BspEI         210  TCCGGA               T^CCGGA
BspLU11I     1273  ACATGT               A^CATGT
Bst1107I      433  GTATAC               GTA^TAC
BstXI        1433  CCACTCCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       433  GTATAC               GTA^TAC
CfoI          522  GCGC                 GCG^C
DrdI         1084  GACCTCAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1739  CCATGG               C^C[GA][CT]GG
EaeI         1601  TGGCCA               [CT]^GGCC[GA]
EcoNI         198  CCTGCGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         509  GAATTC               G^AATTC
FauI          126  GCGGG                CCCGC(4/6)
HhaI          522  GCGC                 GCG^C
HinP1I        522  GCGC                 G^CGC
HpaI         1831  GTTAAC               GTT^AAC
MaeII        1228  ACGT                 A^CGT
MluNI        1601  TGGCCA               TGG^CCA
MroI          210  TCCGGA               T^CCGGA
MscI         1601  TGGCCA               TGG^CCA
NciI          983  CCCGG                CC^[GC]GG
NcoI         1739  CCATGG               C^CATGG
PleI         1911  GACTC                GAGTC(4/5)
PshAI         397  GACAATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1705  ATTAAT               AT^TAAT
SapI           94  GCTCTTC              GCTCTTC(1/4)
SpeI          804  ACTAGT               A^CTAGT
StuI          703  AGGCCT               AGG^CCT
XbaI          876  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BlpI          933  GCTCAGC              GC^T[ACGT]AGC
             1111  GCTGAGC             
Bpu1102I      933  GCTCAGC              GC^T[ACGT]AGC
             1111  GCTGAGC             
BsaWI         210  TCCGGA               [AT]^CCGG[AT]
             1059  TCCGGT              
BsmFI         756  GTCCC                GGGAC(10/14)
             1430  GTCCC               
Bsp1407I     1268  TGTACA               T^GTACA
             1902  TGTACA              
BsrBI         649  CCGCTC               CCGCTC(-3/-3)
              880  GAGCGG              
BsrGI        1268  TGTACA               T^GTACA
             1902  TGTACA              
BstYI         672  GGATCT               [GA]^GATC[CT]
             1544  GGATCC              
CelII         933  GCTCAGC              GC^T[ACGT]AGC
             1111  GCTGAGC             
EarI           95  CTCTTC               CTCTTC(1/4)
              856  CTCTTC              
Ksp632I        95  CTCTTC               CTCTTC(1/4)
              856  CTCTTC              
MflI          672  GGATCT               [GA]^GATC[CT]
             1544  GGATCC              
MspA1I        936  CAGCTG               C[AC]G^C[GT]G
             1109  CAGCTG              
PpuMI          42  AGGTCCT              [GA]G^G[AT]CC[CT]
             1428  GGGTCCC             
PvuII         936  CAGCTG               CAG^CTG
             1109  CAGCTG              
SacI          454  GAGCTC               GAGCT^C
             1027  GAGCTC              
SalI         1444  GTCGAC               G^TCGAC
             1497  GTCGAC              
SmlI          294  CTTGAG               C^T[CT][GA]AG
              594  CTTGAG              
SspBI        1268  TGTACA               T^GTACA
             1902  TGTACA              
TfiI          684  GAATC                G^A[AT]TC
              762  GATTC               
XcmI         1438  CCAGTGGTCGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1604  CCATCACACTCATGG     
XhoII         672  GGATCT               [GA]^GATC[CT]
             1544  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspHI       AvaII       
BanII       BbvI        BfaI        BmyI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BspHI       BspMI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PstI        RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XmnI        
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