Restriction map for pF1KSDA1580
 (Linear) (Six-base) MAPSORT of: KIAA1580.seq  Check: 834  from: 1  to: 1920



KIAA1580.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1760    1920 
   Size:       1760     160
 
AccI GT'mk_AC

Cuts at:      0     434    1445    1498    1920 
   Size:        434    1011      53     422

  Fragments arranged by size: 

                1011     434     422      53
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     781    1118    1295    1920 
   Size:        469     312     337     177     625

  Fragments arranged by size: 

                 625     469     337     312     177
 
AflIII A'CryG_T

Cuts at:      0    1273    1920 
   Size:       1273     647
 
AleI CACnn'nnGTG

Cuts at:      0    1438    1462    1920 
   Size:       1438      24     458

  Fragments arranged by size: 

                1438     458      24
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1528    1920 
   Size:       1528     392
 
ApaI G_GGCC'C

Cuts at:      0      63    1920 
   Size:         63    1857
 
ApoI r'AATT_y

Cuts at:      0     509    1233    1842    1920 
   Size:        509     724     609      78

  Fragments arranged by size: 

                 724     609     509      78
 
AseI AT'TA_AT

Cuts at:      0    1706    1920 
   Size:       1706     214
 
AvrII C'CTAG_G

Cuts at:      0      46    1920 
   Size:         46    1874
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1133    1166    1920 
   Size:       1133      33     754

  Fragments arranged by size: 

                1133     754      33
 
BamHI G'GATC_C

Cuts at:      0    1544    1920 
   Size:       1544     376
 
BanI G'GyrC_C

Cuts at:      0     571    1920 
   Size:        571    1349
 
BanII G_rGCy'C

Cuts at:      0      63     132     458    1031    1076    1920 
   Size:         63      69     326     573      45     844

  Fragments arranged by size: 

                 844     573     326      69      63      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1576    1920 
   Size:       1576     344
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1645    1679    1920 
   Size:       1645      34     241

  Fragments arranged by size: 

                1645     241      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     878    1920 
   Size:        878    1042
 
BlpI GC'TnA_GC

Cuts at:      0     934    1112    1920 
   Size:        934     178     808

  Fragments arranged by size: 

                 934     808     178
 
Bme1580I G_kGCm'C

Cuts at:      0      63     984    1920 
   Size:         63     921     936

  Fragments arranged by size: 

                 936     921      63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1458    1516    1920 
   Size:       1458      58     404

  Fragments arranged by size: 

                1458     404      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1421    1920 
   Size:       1421     499
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     315     615    1920 
   Size:        315     300    1305

  Fragments arranged by size: 

                1305     315     300
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1447    1920 
   Size:       1447     473
 
BsaWI w'CCGG_w

Cuts at:      0     210    1059    1920 
   Size:        210     849     861

  Fragments arranged by size: 

                 861     849     210
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     416     445     446     475    1329    1359    1920 
   Size:        416      29       1      29     854      30     561

  Fragments arranged by size: 

                 854     561     416      30      29      29       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     986    1920 
   Size:        986     934
 
BsiHKAI G_wGCw'C

Cuts at:      0     154     458    1031    1217    1492    1781    1920 
   Size:        154     304     573     186     275     289     139

  Fragments arranged by size: 

                 573     304     289     275     186     154     139
 
BsmI GAATG_Cn'

Cuts at:      0     786    1920 
   Size:        786    1134
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1397    1920 
   Size:       1397     523
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     132     154     458     984    1031    1076    1217 
   Size:         63      69      22     304     526      47      45     141

Cuts at:   1217    1492    1781    1920 
   Size:        275     289     139

  Fragments arranged by size: 

                 526     304     289     275     141     139      69      63
                  47      45      22
 
BspEI T'CCGG_A

Cuts at:      0     210    1920 
   Size:        210    1710
 
BspHI T'CATG_A

Cuts at:      0     849    1178    1565    1920 
   Size:        849     329     387     355

  Fragments arranged by size: 

                 849     387     355     329
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     145     206    1760    1920 
   Size:        145      61    1554     160

  Fragments arranged by size: 

                1554     160     145      61
 
BsrBI CCG'CTC

Cuts at:      0     651     882    1920 
   Size:        651     231    1038

  Fragments arranged by size: 

                1038     651     231
 
BsrGI T'GTAC_A

Cuts at:      0    1268    1902    1920 
   Size:       1268     634      18

  Fragments arranged by size: 

                1268     634      18
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1440    1920 
   Size:       1440     480
 
BstYI r'GATC_y

Cuts at:      0     672    1544    1920 
   Size:        672     872     376

  Fragments arranged by size: 

                 872     672     376
 
BstZ17I GTA'TAC

Cuts at:      0     435    1920 
   Size:        435    1485
 
BtgI C'CryG_G

Cuts at:      0    1739    1920 
   Size:       1739     181
 
BtsI GCAGTG_nn'

Cuts at:      0    1628    1920 
   Size:       1628     292
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     724     759    1920 
   Size:        724      35    1161

  Fragments arranged by size: 

                1161     724      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1090    1920 
   Size:       1090     830
 
EaeI y'GGCC_r

Cuts at:      0    1601    1920 
   Size:       1601     319
 
EarI CTCTTCn'nnn_

Cuts at:      0     101     862    1920 
   Size:        101     761    1058

  Fragments arranged by size: 

                1058     761     101
 
EcoICRI GAG'CTC

Cuts at:      0     456    1029    1920 
   Size:        456     573     891

  Fragments arranged by size: 

                 891     573     456
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     781    1118    1295    1421    1920 
   Size:        469     312     337     177     126     499

  Fragments arranged by size: 

                 499     469     337     312     177     126
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     202    1920 
   Size:        202    1718
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43      59      60    1429    1920 
   Size:         43      16       1    1369     491

  Fragments arranged by size: 

                1369     491      43      16       1
 
EcoRI G'AATT_C

Cuts at:      0     509    1920 
   Size:        509    1411
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      33     506    1920 
   Size:         33     473    1414

  Fragments arranged by size: 

                1414     473      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       6      38     205     237    1458    1490    1920 
   Size:          6      32     167      32    1221      32     430

  Fragments arranged by size: 

                1221     430     167      32      32      32       6
 
HincII GTy'rAC

Cuts at:      0    1446    1499    1833    1920 
   Size:       1446      53     334      87

  Fragments arranged by size: 

                1446     334      87      53
 
HpaI GTT'AAC

Cuts at:      0    1833    1920 
   Size:       1833      87
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     614    1920 
   Size:        614    1306
 
MscI TGG'CCA

Cuts at:      0    1603    1920 
   Size:       1603     317
 
MslI CAynn'nnrTG

Cuts at:      0     274    1276    1438    1462    1522    1612    1738    1920 
   Size:        274    1002     162      24      60      90     126     182

  Fragments arranged by size: 

                1002     274     182     162     126      90      60      24
            
 
MspA1I CmG'CkG

Cuts at:      0     938    1111    1920 
   Size:        938     173     809

  Fragments arranged by size: 

                 938     809     173
 
NcoI C'CATG_G

Cuts at:      0    1739    1920 
   Size:       1739     181
 
NspI r_CATG'y

Cuts at:      0    1052    1269    1277    1920 
   Size:       1052     217       8     643

  Fragments arranged by size: 

                1052     643     217       8
 
PciI A'CATG_T

Cuts at:      0    1273    1920 
   Size:       1273     647
 
PpuMI rG'GwC_Cy

Cuts at:      0      43    1429    1920 
   Size:         43    1386     491

  Fragments arranged by size: 

                1386     491      43
 
PshAI GACnn'nnGTC

Cuts at:      0     401    1920 
   Size:        401    1519
 
PspOMI G'GGCC_C

Cuts at:      0      59    1920 
   Size:         59    1861
 
PstI C_TGCA'G

Cuts at:      0     160    1083    1335    1623    1920 
   Size:        160     923     252     288     297

  Fragments arranged by size: 

                 923     297     288     252     160
 
PvuII CAG'CTG

Cuts at:      0     938    1111    1920 
   Size:        938     173     809

  Fragments arranged by size: 

                 938     809     173
 
SacI G_AGCT'C

Cuts at:      0     458    1031    1920 
   Size:        458     573     889

  Fragments arranged by size: 

                 889     573     458
 
SalI G'TCGA_C

Cuts at:      0    1444    1497    1920 
   Size:       1444      53     423

  Fragments arranged by size: 

                1444     423      53
 
SanDI GG'GwC_CC

Cuts at:      0    1429    1920 
   Size:       1429     491
 
SapI GCTCTTCn'nnn_

Cuts at:      0     101    1920 
   Size:        101    1819
 
SfcI C'TryA_G

Cuts at:      0     156     313    1079    1331    1619    1920 
   Size:        156     157     766     252     288     301

  Fragments arranged by size: 

                 766     301     288     252     157     156
 
SmlI C'TyrA_G

Cuts at:      0     294     594    1920 
   Size:        294     300    1326

  Fragments arranged by size: 

                1326     300     294
 
SpeI A'CTAG_T

Cuts at:      0     804    1920 
   Size:        804    1116
 
StuI AGG'CCT

Cuts at:      0     705    1920 
   Size:        705    1215
 
StyI C'CwwG_G

Cuts at:      0      46     904    1739    1920 
   Size:         46     858     835     181

  Fragments arranged by size: 

                 858     835     181      46
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1580    1752    1920 
   Size:       1580     172     168

  Fragments arranged by size: 

                1580     172     168
 
TatI w'GTAC_w

Cuts at:      0    1268    1902    1920 
   Size:       1268     634      18

  Fragments arranged by size: 

                1268     634      18
 
XbaI T'CTAG_A

Cuts at:      0     876    1920 
   Size:        876    1044
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1445    1611    1920 
   Size:       1445     166     309

  Fragments arranged by size: 

                1445     309     166
 
XmnI GAAnn'nnTTC

Cuts at:      0     283     513    1042    1510    1920 
   Size:        283     230     529     468     410

  Fragments arranged by size: 

                 529     468     410     283     230
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI    AlwNI     ApaI     ApoI
     AseI    AvrII     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BlpI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaWI
    BsaXI    BseRI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrGI    BstXI    BstYI  BstZ17I     BtgI     BtsI
    CspCI     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    Hin4I   HincII     HpaI     MmeI     MscI     MslI
   MspA1I     NcoI     NspI     PciI    PpuMI    PshAI   PspOMI     PstI
    PvuII     SacI     SalI    SanDI     SapI     SfcI     SmlI     SpeI
     StuI     StyI    TaqII     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI    ApaLI     AscI    AsiSI     AvaI     BbeI    BbvCI     BclI
    BfrBI     BglI    BglII    BmgBI     BmtI     BplI   Bpu10I    BsaAI
    BsaBI    BsaHI    BseYI     BsgI    BsiEI    BsiWI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I    BtgZI     ClaI
     DraI   DraIII     EagI     EciI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     SwaI
     TstI  Tth111I     XhoI     XmaI     ZraI
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