Restriction map for pF1KSDA1576
 (Linear) (Six-base) MAPSORT of: KIAA1576.seq  Check: 8846  from: 1  to: 1257



KIAA1576.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     879    1257 
   Size:        879     378
 
AccI GT'mk_AC

Cuts at:      0     437    1257 
   Size:        437     820
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     752     849    1257 
   Size:        159     593      97     408

  Fragments arranged by size: 

                 593     408     159      97
 
AleI CACnn'nnGTG

Cuts at:      0     424    1257 
   Size:        424     833
 
ApaLI G'TGCA_C

Cuts at:      0     556    1257 
   Size:        556     701
 
AvaI C'yCGr_G

Cuts at:      0     106     190    1257 
   Size:        106      84    1067

  Fragments arranged by size: 

                1067     106      84
 
BanI G'GyrC_C

Cuts at:      0     300    1257 
   Size:        300     957
 
BanII G_rGCy'C

Cuts at:      0     158     212    1022    1068    1257 
   Size:        158      54     810      46     189

  Fragments arranged by size: 

                 810     189     158      54      46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     784    1257 
   Size:        784     473
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     457     491    1257 
   Size:        457      34     766

  Fragments arranged by size: 

                 766     457      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     983    1257 
   Size:        983     274
 
BlpI GC'TnA_GC

Cuts at:      0     593    1257 
   Size:        593     664
 
Bme1580I G_kGCm'C

Cuts at:      0     560    1257 
   Size:        560     697
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     866    1090    1257 
   Size:        866     224     167

  Fragments arranged by size: 

                 866     224     167
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196    1257 
   Size:        196    1061
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     362     790     844    1174    1257 
   Size:        362     428      54     330      83

  Fragments arranged by size: 

                 428     362     330      83      54
 
BsaAI yAC'GTr

Cuts at:      0     705    1257 
   Size:        705     552
 
BsaHI Gr'CG_yC

Cuts at:      0     113    1257 
   Size:        113    1144
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     359     389    1137    1167    1257 
   Size:        359      30     748      30      90

  Fragments arranged by size: 

                 748     359      90      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     937    1087    1216    1219    1257 
   Size:         43     894     150     129       3      38

  Fragments arranged by size: 

                 894     150     129      43      38       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     402    1257 
   Size:        402     855
 
BsiEI CG_ry'CG

Cuts at:      0    1107    1257 
   Size:       1107     150
 
BsiHKAI G_wGCw'C

Cuts at:      0     212     554     560    1257 
   Size:        212     342       6     697

  Fragments arranged by size: 

                 697     342     212       6
 
BsmI GAATG_Cn'

Cuts at:      0     478    1257 
   Size:        478     779
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      25    1257 
   Size:         25    1232
 
Bsp1286I G_dGCh'C

Cuts at:      0     158     212     554     560    1022    1068    1257 
   Size:        158      54     342       6     462      46     189

  Fragments arranged by size: 

                 462     342     189     158      54      46       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     879    1257 
   Size:        879     378
 
BsrBI CCG'CTC

Cuts at:      0    1239    1257 
   Size:       1239      18
 
BsrFI r'CCGG_y

Cuts at:      0      69    1257 
   Size:         69    1188
 
BssHII G'CGCG_C

Cuts at:      0     126    1257 
   Size:        126    1131
 
BssSI C'ACGA_G

Cuts at:      0     553    1213    1257 
   Size:        553     660      44

  Fragments arranged by size: 

                 660     553      44
 
BstYI r'GATC_y

Cuts at:      0     215     443    1257 
   Size:        215     228     814

  Fragments arranged by size: 

                 814     228     215
 
Bsu36I CC'TnA_GG

Cuts at:      0     197    1257 
   Size:        197    1060
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     921    1257 
   Size:        921     336
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     213     248    1257 
   Size:        213      35    1009

  Fragments arranged by size: 

                1009     213      35
 
DraI TTT'AAA

Cuts at:      0     956    1257 
   Size:        956     301
 
EaeI y'GGCC_r

Cuts at:      0       2    1163    1257 
   Size:          2    1161      94

  Fragments arranged by size: 

                1161      94       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     178     970    1193    1257 
   Size:        178     792     223      64

  Fragments arranged by size: 

                 792     223     178      64
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      40      88    1257 
   Size:         40      48    1169

  Fragments arranged by size: 

                1169      48      40
 
EcoICRI GAG'CTC

Cuts at:      0     210    1257 
   Size:        210    1047
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     752     849     866    1090    1257 
   Size:        159     593      97      17     224     167

  Fragments arranged by size: 

                 593     224     167     159      97      17
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     879    1257 
   Size:        879     378
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     359     391    1137    1169    1257 
   Size:        359      32     746      32      88

  Fragments arranged by size: 

                 746     359      88      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     857    1257 
   Size:        857     400
 
MscI TGG'CCA

Cuts at:      0       4    1165    1257 
   Size:          4    1161      92

  Fragments arranged by size: 

                1161      92       4
 
MslI CAynn'nnrTG

Cuts at:      0     424    1257 
   Size:        424     833
 
MspA1I CmG'CkG

Cuts at:      0     565     597    1095    1257 
   Size:        565      32     498     162

  Fragments arranged by size: 

                 565     498     162      32
 
NaeI GCC'GGC

Cuts at:      0      71    1257 
   Size:         71    1186
 
NgoMIV G'CCGG_C

Cuts at:      0      69    1257 
   Size:         69    1188
 
PasI CC'CwG_GG

Cuts at:      0     804    1257 
   Size:        804     453
 
PfoI T'CCnGG_A

Cuts at:      0     446     533    1257 
   Size:        446      87     724

  Fragments arranged by size: 

                 724     446      87
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     624     656    1257 
   Size:        624      32     601

  Fragments arranged by size: 

                 624     601      32
 
PvuII CAG'CTG

Cuts at:      0     565     597    1257 
   Size:        565      32     660

  Fragments arranged by size: 

                 660     565      32
 
SacI G_AGCT'C

Cuts at:      0     212    1257 
   Size:        212    1045
 
SapI GCTCTTCn'nnn_

Cuts at:      0     178     970    1257 
   Size:        178     792     287

  Fragments arranged by size: 

                 792     287     178
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     983    1257 
   Size:        983     274
 
SmlI C'TyrA_G

Cuts at:      0     211    1257 
   Size:        211    1046
 
SspI AAT'ATT

Cuts at:      0     273    1257 
   Size:        273     984
 
StyI C'CwwG_G

Cuts at:      0       5    1257 
   Size:          5    1252
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1121    1257 
   Size:       1121     136
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1045    1077    1257 
   Size:       1045      32     180

  Fragments arranged by size: 

                1045     180      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     373    1257 
   Size:        373     884
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      12     294    1257 
   Size:         12     282     963

  Fragments arranged by size: 

                 963     282      12
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI    ApaLI     AvaI     BanI    BanII
     BbsI     BcgI     BglI     BlpI Bme1580I     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstYI
   Bsu36I    BtgZI    CspCI     DraI     EaeI     EarI     EciI  EcoICRI
  Eco57MI     FalI    Hin4I     MmeI     MscI     MslI   MspA1I     NaeI
   NgoMIV     PasI     PfoI     PsrI    PvuII     SacI     SapI     SfiI
     SmlI     SspI     StyI    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaBI    BsaWI
    BseYI    BsiWI    BspEI    BspHI    BsrDI    BsrGI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtgI     BtsI     ClaI   DraIII     DrdI
     EagI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI
     XbaI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com