(Circular) (Six-base) MAPSORT of: pF1KSDA1479.seq Check: 647 from: 1 to: 6170 pF1KSDA1479 6170 bp With 182 enzymes: SgfI * June 20, 2008 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1966 1966 Size: 6170 AccI GT'mk_AC Cuts at: 224 1988 2266 3208 224 Size: 1764 278 942 3186 Fragments arranged by size: 3186 1764 942 278 Acc65I G'GTAC_C Cuts at: 535 3186 535 Size: 2651 3519 AclI AA'CG_TT Cuts at: 6010 6010 Size: 6170 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 218 254 1475 1835 2608 3995 4427 5174 5442 Size: 36 1221 360 773 1387 432 747 268 Cuts at: 5442 218 Size: 946 Fragments arranged by size: 1387 1221 946 773 747 432 360 268 36 AflIII A'CryG_T Cuts at: 2003 2453 3522 4626 5603 2003 Size: 450 1069 1104 977 2570 Fragments arranged by size: 2570 1104 1069 977 450 AgeI A'CCGG_T Cuts at: 4558 4558 Size: 6170 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2007 2007 Size: 6170 AlwNI CAG_nnn'CTG Cuts at: 691 1180 1667 3475 5042 691 Size: 489 487 1808 1567 1819 Fragments arranged by size: 1819 1808 1567 489 487 ApaLI G'TGCA_C Cuts at: 4940 4940 Size: 6170 ApoI r'AATT_y Cuts at: 317 1837 2838 3174 5523 5779 317 Size: 1520 1001 336 2349 256 708 Fragments arranged by size: 2349 1520 1001 708 336 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6170 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6170 AvaI C'yCGr_G Cuts at: 1370 3190 1370 Size: 1820 4350 AvrII C'CTAG_G Cuts at: 3052 3052 Size: 6170 BamHI G'GATC_C Cuts at: 1741 2667 3195 1741 Size: 926 528 4716 Fragments arranged by size: 4716 926 528 BanI G'GyrC_C Cuts at: 535 3186 3600 3848 3883 535 Size: 2651 414 248 35 2822 Fragments arranged by size: 2822 2651 414 248 35 BanII G_rGCy'C Cuts at: 1355 3184 4214 1355 Size: 1829 1030 3311 Fragments arranged by size: 3311 1829 1030 BbeI G_GCGC'C Cuts at: 3604 3852 3604 Size: 248 5922 BbsI GAAGACnn'nnnn_ Cuts at: 126 1208 1580 1790 2293 126 Size: 1082 372 210 503 4003 Fragments arranged by size: 4003 1082 503 372 210 BciVI GTATCCnnnnn_n' Cuts at: 4068 4829 6170 4068 Size: 761 1341 4068 Fragments arranged by size: 4068 1341 761 BclI T'GATC_A Cuts at: 2508 3690 2508 Size: 1182 4988 BfrBI ATG'CAT Cuts at: 544 544 Size: 6170 BglI GCCn_nnn'nGGC Cuts at: 272 688 272 Size: 416 5754 BglII A'GATC_T Cuts at: 3685 5722 3685 Size: 2037 4133 BlpI GC'TnA_GC Cuts at: 3282 3282 Size: 6170 Bme1580I G_kGCm'C Cuts at: 3795 3888 4944 3795 Size: 93 1056 5021 Fragments arranged by size: 5021 1056 93 BmgBI CAC'GTC Cuts at: 2456 3093 2456 Size: 637 5533 BmrI ACTGGGnnnn_n' Cuts at: 168 2046 2408 2963 3382 3463 3798 5942 168 Size: 1878 362 555 419 81 335 2144 396 Fragments arranged by size: 2144 1878 555 419 396 362 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4537 4537 Size: 6170 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 955 1007 1157 1916 2052 2219 2813 3000 955 Size: 52 150 759 136 167 594 187 4125 Fragments arranged by size: 4125 759 594 187 167 150 136 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3346 4225 4717 5015 3346 Size: 879 492 298 4501 Fragments arranged by size: 4501 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 2101 5874 2101 Size: 3773 2397 BsaAI yAC'GTr Cuts at: 3162 3523 3162 Size: 361 5809 BsaBI GATnn'nnATC Cuts at: 6 1607 3684 5721 6 Size: 1601 2077 2037 455 Fragments arranged by size: 2077 2037 1601 455 BsaHI Gr'CG_yC Cuts at: 264 3073 3601 3849 4551 6047 264 Size: 2809 528 248 702 1496 387 Fragments arranged by size: 2809 1496 702 528 387 248 BsaWI w'CCGG_w Cuts at: 249 3359 3880 4558 4832 4979 5390 249 Size: 3110 521 678 274 147 411 1029 Fragments arranged by size: 3110 1029 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 923 3595 4353 4930 5426 923 Size: 2672 758 577 496 1667 Fragments arranged by size: 2672 1667 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1575 1575 Size: 6170 BsiEI CG_ry'CG Cuts at: 104 3712 3758 4966 104 Size: 3608 46 1208 1308 Fragments arranged by size: 3608 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 3184 3962 4152 4944 3184 Size: 778 190 792 4410 Fragments arranged by size: 4410 792 778 190 BsmI GAATG_Cn' Cuts at: 481 544 1966 481 Size: 63 1422 4685 Fragments arranged by size: 4685 1422 63 Bsp1286I G_dGCh'C Cuts at: 1355 3184 3795 3888 3962 4152 4214 4944 1355 Size: 1829 611 93 74 190 62 730 2581 Fragments arranged by size: 2581 1829 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 5390 5390 Size: 6170 BspHI T'CATG_A Cuts at: 5346 5346 Size: 6170 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1966 2168 3220 3434 3736 4117 1966 Size: 202 1052 214 302 381 4019 Fragments arranged by size: 4019 1052 381 302 214 202 BsrBI CCG'CTC Cuts at: 50 4462 4516 6000 6165 50 Size: 4412 54 1484 165 55 Fragments arranged by size: 4412 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 282 1340 1970 4082 282 Size: 1058 630 2112 2370 Fragments arranged by size: 2370 2112 1058 630 BsrFI r'CCGG_y Cuts at: 2546 4168 4349 4558 2546 Size: 1622 181 209 4158 Fragments arranged by size: 4158 1622 209 181 BssSI C'ACGA_G Cuts at: 1709 4441 4799 1709 Size: 2732 358 3080 Fragments arranged by size: 3080 2732 358 BstAPI GCAn_nnn'nTGC Cuts at: 1972 3547 1972 Size: 1575 4595 BstBI TT'CG_AA Cuts at: 110 4531 110 Size: 4421 1749 BstXI CCAn_nnnn'nTGG Cuts at: 1401 1911 2214 2332 2772 1401 Size: 510 303 118 440 4799 Fragments arranged by size: 4799 510 440 303 118 BstYI r'GATC_y Cuts at: 1 739 1741 2027 2667 3195 3662 3685 4020 Size: 738 1002 286 640 528 467 23 335 Cuts at: 4020 4266 5278 5364 5376 5722 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 1002 738 640 528 467 449 346 335 286 246 86 23 12 BstZ17I GTA'TAC Cuts at: 1989 1989 Size: 6170 BtgI C'CryG_G Cuts at: 1869 2207 2325 2331 2672 2928 1869 Size: 338 118 6 341 256 5111 Fragments arranged by size: 5111 341 338 256 118 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 722 2705 4120 4301 4464 722 Size: 1983 1415 181 163 2428 Fragments arranged by size: 2428 1983 1415 181 163 BtsI GCAGTG_nn' Cuts at: 1563 3413 1563 Size: 1850 4320 ClaI AT'CG_AT Cuts at: 1274 2680 1274 Size: 1406 4764 DraI TTT'AAA Cuts at: 3169 3169 Size: 6170 DrdI GACnn_nn'nnGTC Cuts at: 1582 2110 3876 4734 1582 Size: 528 1766 858 3018 Fragments arranged by size: 3018 1766 858 528 EaeI y'GGCC_r Cuts at: 701 1391 2328 3755 3929 4320 4347 5692 701 Size: 690 937 1427 174 391 27 1345 1179 Fragments arranged by size: 1427 1345 1179 937 690 391 174 27 EagI C'GGCC_G Cuts at: 3755 3755 Size: 6170 EarI CTCTTCn'nnn_ Cuts at: 1520 2255 2299 3053 4193 4403 1520 Size: 735 44 754 1140 210 3287 Fragments arranged by size: 3287 1140 754 735 210 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2037 2366 4378 4688 4834 5980 2037 Size: 329 2012 310 146 1146 2227 Fragments arranged by size: 2227 2012 1146 329 310 146 EcoICRI GAG'CTC Cuts at: 3182 3182 Size: 6170 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 218 254 955 1007 1157 1475 1835 1916 2052 Size: 36 701 52 150 318 360 81 136 Cuts at: 2052 2219 2608 2813 3000 3995 4427 5174 5442 Size: 167 389 205 187 995 432 747 268 Cuts at: 5442 218 Size: 946 Fragments arranged by size: 995 946 747 701 432 389 360 318 268 205 187 167 150 136 81 52 36 EcoNI CCTnn'n_nnAGG Cuts at: 2847 2847 Size: 6170 EcoO109I rG'GnC_Cy Cuts at: 930 2691 3309 930 Size: 1761 618 3791 Fragments arranged by size: 3791 1761 618 EcoRI G'AATT_C Cuts at: 1837 3174 1837 Size: 1337 4833 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3126 4189 3126 Size: 1063 5107 FspI TGC'GCA Cuts at: 3951 3951 Size: 6170 HaeII r_GCGC'y Cuts at: 3604 3852 4874 5853 3604 Size: 248 1022 979 3921 Fragments arranged by size: 3921 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1871 1903 2029 2061 2188 2220 2762 2794 2982 Size: 32 126 32 127 32 542 32 188 Cuts at: 2982 3014 4012 4042 4044 4074 1871 Size: 32 998 30 2 30 3967 Fragments arranged by size: 3967 998 542 188 127 126 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 225 1778 3209 225 Size: 1553 1431 3186 Fragments arranged by size: 3186 1553 1431 HindIII A'AGCT_T Cuts at: 1256 2494 1256 Size: 1238 4932 HpaI GTT'AAC Cuts at: 1778 1778 Size: 6170 KasI G'GCGC_C Cuts at: 3600 3848 3600 Size: 248 5922 KpnI G_GTAC'C Cuts at: 539 3190 539 Size: 2651 3519 MluI A'CGCG_T Cuts at: 5603 5603 Size: 6170 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1580 1928 2152 4841 5025 1580 Size: 348 224 2689 184 2725 Fragments arranged by size: 2725 2689 348 224 184 MscI TGG'CCA Cuts at: 703 2330 3931 703 Size: 1627 1601 2942 Fragments arranged by size: 2942 1627 1601 MslI CAynn'nnrTG Cuts at: 470 1399 1922 2036 2212 2330 2597 2641 4159 Size: 929 523 114 176 118 267 44 1518 Cuts at: 4159 4286 470 Size: 127 2354 Fragments arranged by size: 2354 1518 929 523 267 176 127 118 114 44 MspA1I CmG'CkG Cuts at: 306 927 3281 3595 3955 4968 5213 306 Size: 621 2354 314 360 1013 245 1263 Fragments arranged by size: 2354 1263 1013 621 360 314 245 NaeI GCC'GGC Cuts at: 4351 4351 Size: 6170 NarI GG'CG_CC Cuts at: 3601 3849 3601 Size: 248 5922 NcoI C'CATG_G Cuts at: 1869 2207 2325 2331 2672 1869 Size: 338 118 6 341 5367 Fragments arranged by size: 5367 341 338 118 6 NdeI CA'TA_TG Cuts at: 898 898 Size: 6170 NgoMIV G'CCGG_C Cuts at: 4349 4349 Size: 6170 NsiI A_TGCA'T Cuts at: 546 546 Size: 6170 NspI r_CATG'y Cuts at: 2007 3223 3723 4630 2007 Size: 1216 500 907 3547 Fragments arranged by size: 3547 1216 907 500 PasI CC'CwG_GG Cuts at: 1762 1762 Size: 6170 PciI A'CATG_T Cuts at: 2003 4626 2003 Size: 2623 3547 PflMI CCAn_nnn'nTGG Cuts at: 1225 2191 1225 Size: 966 5204 PfoI T'CCnGG_A Cuts at: 932 1280 2790 932 Size: 348 1510 4312 Fragments arranged by size: 4312 1510 348 PmeI GTTT'AAAC Cuts at: 3169 3169 Size: 6170 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1342 1374 5309 5341 1342 Size: 32 3935 32 2171 Fragments arranged by size: 3935 2171 32 32 PpuMI rG'GwC_Cy Cuts at: 930 2691 930 Size: 1761 4409 PshAI GACnn'nnGTC Cuts at: 1372 1372 Size: 6170 PsiI TTA'TAA Cuts at: 1262 1262 Size: 6170 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1460 1492 1460 Size: 32 6138 PstI C_TGCA'G Cuts at: 1019 2297 3005 3217 3902 1019 Size: 1278 708 212 685 3287 Fragments arranged by size: 3287 1278 708 685 212 PvuI CG_AT'CG Cuts at: 104 104 Size: 6170 PvuII CAG'CTG Cuts at: 306 3595 3955 306 Size: 3289 360 2521 Fragments arranged by size: 3289 2521 360 RsrII CG'GwC_CG Cuts at: 4365 4365 Size: 6170 SacI G_AGCT'C Cuts at: 3184 3184 Size: 6170 SalI G'TCGA_C Cuts at: 223 3207 223 Size: 2984 3186 SapI GCTCTTCn'nnn_ Cuts at: 2255 2299 4193 4403 2255 Size: 44 1894 210 4022 Fragments arranged by size: 4022 1894 210 44 SbfI CC_TGCA'GG Cuts at: 2297 3217 2297 Size: 920 5250 ScaI AGT'ACT Cuts at: 5687 5687 Size: 6170 SfcI C'TryA_G Cuts at: 33 567 1015 2293 3001 3213 3898 4891 5082 Size: 534 448 1278 708 212 685 993 191 Cuts at: 5082 33 Size: 1121 Fragments arranged by size: 1278 1121 993 708 685 534 448 212 191 SfoI GGC'GCC Cuts at: 3602 3850 3602 Size: 248 5922 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6170 SgrAI Cr'CCGG_yG Cuts at: 2546 2546 Size: 6170 SmaI CCC'GGG Cuts at: 3192 3192 Size: 6170 SmlI C'TyrA_G Cuts at: 3325 4240 4732 4994 3325 Size: 915 492 262 4501 Fragments arranged by size: 4501 915 492 262 SnaBI TAC'GTA Cuts at: 3162 3162 Size: 6170 SpeI A'CTAG_T Cuts at: 67 67 Size: 6170 SphI G_CATG'C Cuts at: 3223 3723 3223 Size: 500 5670 SspI AAT'ATT Cuts at: 2133 2133 Size: 6170 StuI AGG'CCT Cuts at: 1224 1224 Size: 6170 StyI C'CwwG_G Cuts at: 1219 1869 2207 2325 2331 2672 3052 3304 3658 Size: 650 338 118 6 341 380 252 354 Cuts at: 3658 1219 Size: 3731 Fragments arranged by size: 3731 650 380 354 341 338 252 118 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 721 904 1353 2202 3753 3886 4345 4554 721 Size: 183 449 849 1551 133 459 209 2337 Fragments arranged by size: 2337 1551 849 459 449 209 183 133 TatI w'GTAC_w Cuts at: 5685 5685 Size: 6170 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2687 2719 2824 2856 2972 3004 5687 5719 2687 Size: 32 105 32 116 32 2683 32 3138 Fragments arranged by size: 3138 2683 116 105 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3075 3967 3075 Size: 892 5278 XbaI T'CTAG_A Cuts at: 3201 3201 Size: 6170 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1181 1744 2625 3665 1181 Size: 563 881 1040 3686 Fragments arranged by size: 3686 1040 881 563 XmaI C'CCGG_G Cuts at: 3190 3190 Size: 6170 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AloI ApaI AscI BaeI BbvCI BcgI BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII Bsu36I CspCI DraIII EcoRV FseI FspAI MfeI NheI NotI NruI PacI PmlI PspOMI PspXI SacII SanDI SexAI SfiI SrfI SwaI XhoI XmnI ZraI