Restriction map for pF1KSDA1479
 (Circular) (Six-base) MAPSORT of: pF1KSDA1479.seq  Check: 647  from: 1  to: 6170



pF1KSDA1479  6170 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1966    1966 
   Size:       6170
 
AccI GT'mk_AC

Cuts at:    224    1988    2266    3208     224 
   Size:       1764     278     942    3186

  Fragments arranged by size: 

                3186    1764     942     278
 
Acc65I G'GTAC_C

Cuts at:    535    3186     535 
   Size:       2651    3519
 
AclI AA'CG_TT

Cuts at:   6010    6010 
   Size:       6170
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    218     254    1475    1835    2608    3995    4427    5174    5442 
   Size:         36    1221     360     773    1387     432     747     268

Cuts at:   5442     218 
   Size:        946

  Fragments arranged by size: 

                1387    1221     946     773     747     432     360     268
                  36
 
AflIII A'CryG_T

Cuts at:   2003    2453    3522    4626    5603    2003 
   Size:        450    1069    1104     977    2570

  Fragments arranged by size: 

                2570    1104    1069     977     450
 
AgeI A'CCGG_T

Cuts at:   4558    4558 
   Size:       6170
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2007    2007 
   Size:       6170
 
AlwNI CAG_nnn'CTG

Cuts at:    691    1180    1667    3475    5042     691 
   Size:        489     487    1808    1567    1819

  Fragments arranged by size: 

                1819    1808    1567     489     487
 
ApaLI G'TGCA_C

Cuts at:   4940    4940 
   Size:       6170
 
ApoI r'AATT_y

Cuts at:    317    1837    2838    3174    5523    5779     317 
   Size:       1520    1001     336    2349     256     708

  Fragments arranged by size: 

                2349    1520    1001     708     336     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6170
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6170
 
AvaI C'yCGr_G

Cuts at:   1370    3190    1370 
   Size:       1820    4350
 
AvrII C'CTAG_G

Cuts at:   3052    3052 
   Size:       6170
 
BamHI G'GATC_C

Cuts at:   1741    2667    3195    1741 
   Size:        926     528    4716

  Fragments arranged by size: 

                4716     926     528
 
BanI G'GyrC_C

Cuts at:    535    3186    3600    3848    3883     535 
   Size:       2651     414     248      35    2822

  Fragments arranged by size: 

                2822    2651     414     248      35
 
BanII G_rGCy'C

Cuts at:   1355    3184    4214    1355 
   Size:       1829    1030    3311

  Fragments arranged by size: 

                3311    1829    1030
 
BbeI G_GCGC'C

Cuts at:   3604    3852    3604 
   Size:        248    5922
 
BbsI GAAGACnn'nnnn_

Cuts at:    126    1208    1580    1790    2293     126 
   Size:       1082     372     210     503    4003

  Fragments arranged by size: 

                4003    1082     503     372     210
 
BciVI GTATCCnnnnn_n'

Cuts at:   4068    4829    6170    4068 
   Size:        761    1341    4068

  Fragments arranged by size: 

                4068    1341     761
 
BclI T'GATC_A

Cuts at:   2508    3690    2508 
   Size:       1182    4988
 
BfrBI ATG'CAT

Cuts at:    544     544 
   Size:       6170
 
BglI GCCn_nnn'nGGC

Cuts at:    272     688     272 
   Size:        416    5754
 
BglII A'GATC_T

Cuts at:   3685    5722    3685 
   Size:       2037    4133
 
BlpI GC'TnA_GC

Cuts at:   3282    3282 
   Size:       6170
 
Bme1580I G_kGCm'C

Cuts at:   3795    3888    4944    3795 
   Size:         93    1056    5021

  Fragments arranged by size: 

                5021    1056      93
 
BmgBI CAC'GTC

Cuts at:   2456    3093    2456 
   Size:        637    5533
 
BmrI ACTGGGnnnn_n'

Cuts at:    168    2046    2408    2963    3382    3463    3798    5942     168 
   Size:       1878     362     555     419      81     335    2144     396

  Fragments arranged by size: 

                2144    1878     555     419     396     362     335      81
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4537    4537 
   Size:       6170
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    955    1007    1157    1916    2052    2219    2813    3000     955 
   Size:         52     150     759     136     167     594     187    4125

  Fragments arranged by size: 

                4125     759     594     187     167     150     136      52
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3346    4225    4717    5015    3346 
   Size:        879     492     298    4501

  Fragments arranged by size: 

                4501     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2101    5874    2101 
   Size:       3773    2397
 
BsaAI yAC'GTr

Cuts at:   3162    3523    3162 
   Size:        361    5809
 
BsaBI GATnn'nnATC

Cuts at:      6    1607    3684    5721       6 
   Size:       1601    2077    2037     455

  Fragments arranged by size: 

                2077    2037    1601     455
 
BsaHI Gr'CG_yC

Cuts at:    264    3073    3601    3849    4551    6047     264 
   Size:       2809     528     248     702    1496     387

  Fragments arranged by size: 

                2809    1496     702     528     387     248
 
BsaWI w'CCGG_w

Cuts at:    249    3359    3880    4558    4832    4979    5390     249 
   Size:       3110     521     678     274     147     411    1029

  Fragments arranged by size: 

                3110    1029     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    923    3595    4353    4930    5426     923 
   Size:       2672     758     577     496    1667

  Fragments arranged by size: 

                2672    1667     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1575    1575 
   Size:       6170
 
BsiEI CG_ry'CG

Cuts at:    104    3712    3758    4966     104 
   Size:       3608      46    1208    1308

  Fragments arranged by size: 

                3608    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   3184    3962    4152    4944    3184 
   Size:        778     190     792    4410

  Fragments arranged by size: 

                4410     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    481     544    1966     481 
   Size:         63    1422    4685

  Fragments arranged by size: 

                4685    1422      63
 
Bsp1286I G_dGCh'C

Cuts at:   1355    3184    3795    3888    3962    4152    4214    4944    1355 
   Size:       1829     611      93      74     190      62     730    2581

  Fragments arranged by size: 

                2581    1829     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   5390    5390 
   Size:       6170
 
BspHI T'CATG_A

Cuts at:   5346    5346 
   Size:       6170
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1966    2168    3220    3434    3736    4117    1966 
   Size:        202    1052     214     302     381    4019

  Fragments arranged by size: 

                4019    1052     381     302     214     202
 
BsrBI CCG'CTC

Cuts at:     50    4462    4516    6000    6165      50 
   Size:       4412      54    1484     165      55

  Fragments arranged by size: 

                4412    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    282    1340    1970    4082     282 
   Size:       1058     630    2112    2370

  Fragments arranged by size: 

                2370    2112    1058     630
 
BsrFI r'CCGG_y

Cuts at:   2546    4168    4349    4558    2546 
   Size:       1622     181     209    4158

  Fragments arranged by size: 

                4158    1622     209     181
 
BssSI C'ACGA_G

Cuts at:   1709    4441    4799    1709 
   Size:       2732     358    3080

  Fragments arranged by size: 

                3080    2732     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1972    3547    1972 
   Size:       1575    4595
 
BstBI TT'CG_AA

Cuts at:    110    4531     110 
   Size:       4421    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1401    1911    2214    2332    2772    1401 
   Size:        510     303     118     440    4799

  Fragments arranged by size: 

                4799     510     440     303     118
 
BstYI r'GATC_y

Cuts at:      1     739    1741    2027    2667    3195    3662    3685    4020 
   Size:        738    1002     286     640     528     467      23     335

Cuts at:   4020    4266    5278    5364    5376    5722       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012    1002     738     640     528     467     449     346
                 335     286     246      86      23      12
 
BstZ17I GTA'TAC

Cuts at:   1989    1989 
   Size:       6170
 
BtgI C'CryG_G

Cuts at:   1869    2207    2325    2331    2672    2928    1869 
   Size:        338     118       6     341     256    5111

  Fragments arranged by size: 

                5111     341     338     256     118       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    722    2705    4120    4301    4464     722 
   Size:       1983    1415     181     163    2428

  Fragments arranged by size: 

                2428    1983    1415     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1563    3413    1563 
   Size:       1850    4320
 
ClaI AT'CG_AT

Cuts at:   1274    2680    1274 
   Size:       1406    4764
 
DraI TTT'AAA

Cuts at:   3169    3169 
   Size:       6170
 
DrdI GACnn_nn'nnGTC

Cuts at:   1582    2110    3876    4734    1582 
   Size:        528    1766     858    3018

  Fragments arranged by size: 

                3018    1766     858     528
 
EaeI y'GGCC_r

Cuts at:    701    1391    2328    3755    3929    4320    4347    5692     701 
   Size:        690     937    1427     174     391      27    1345    1179

  Fragments arranged by size: 

                1427    1345    1179     937     690     391     174      27
            
 
EagI C'GGCC_G

Cuts at:   3755    3755 
   Size:       6170
 
EarI CTCTTCn'nnn_

Cuts at:   1520    2255    2299    3053    4193    4403    1520 
   Size:        735      44     754    1140     210    3287

  Fragments arranged by size: 

                3287    1140     754     735     210      44
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2037    2366    4378    4688    4834    5980    2037 
   Size:        329    2012     310     146    1146    2227

  Fragments arranged by size: 

                2227    2012    1146     329     310     146
 
EcoICRI GAG'CTC

Cuts at:   3182    3182 
   Size:       6170
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    218     254     955    1007    1157    1475    1835    1916    2052 
   Size:         36     701      52     150     318     360      81     136

Cuts at:   2052    2219    2608    2813    3000    3995    4427    5174    5442 
   Size:        167     389     205     187     995     432     747     268

Cuts at:   5442     218 
   Size:        946

  Fragments arranged by size: 

                 995     946     747     701     432     389     360     318
                 268     205     187     167     150     136      81      52
                  36
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2847    2847 
   Size:       6170
 
EcoO109I rG'GnC_Cy

Cuts at:    930    2691    3309     930 
   Size:       1761     618    3791

  Fragments arranged by size: 

                3791    1761     618
 
EcoRI G'AATT_C

Cuts at:   1837    3174    1837 
   Size:       1337    4833
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3126    4189    3126 
   Size:       1063    5107
 
FspI TGC'GCA

Cuts at:   3951    3951 
   Size:       6170
 
HaeII r_GCGC'y

Cuts at:   3604    3852    4874    5853    3604 
   Size:        248    1022     979    3921

  Fragments arranged by size: 

                3921    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1871    1903    2029    2061    2188    2220    2762    2794    2982 
   Size:         32     126      32     127      32     542      32     188

Cuts at:   2982    3014    4012    4042    4044    4074    1871 
   Size:         32     998      30       2      30    3967

  Fragments arranged by size: 

                3967     998     542     188     127     126      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:    225    1778    3209     225 
   Size:       1553    1431    3186

  Fragments arranged by size: 

                3186    1553    1431
 
HindIII A'AGCT_T

Cuts at:   1256    2494    1256 
   Size:       1238    4932
 
HpaI GTT'AAC

Cuts at:   1778    1778 
   Size:       6170
 
KasI G'GCGC_C

Cuts at:   3600    3848    3600 
   Size:        248    5922
 
KpnI G_GTAC'C

Cuts at:    539    3190     539 
   Size:       2651    3519
 
MluI A'CGCG_T

Cuts at:   5603    5603 
   Size:       6170
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1580    1928    2152    4841    5025    1580 
   Size:        348     224    2689     184    2725

  Fragments arranged by size: 

                2725    2689     348     224     184
 
MscI TGG'CCA

Cuts at:    703    2330    3931     703 
   Size:       1627    1601    2942

  Fragments arranged by size: 

                2942    1627    1601
 
MslI CAynn'nnrTG

Cuts at:    470    1399    1922    2036    2212    2330    2597    2641    4159 
   Size:        929     523     114     176     118     267      44    1518

Cuts at:   4159    4286     470 
   Size:        127    2354

  Fragments arranged by size: 

                2354    1518     929     523     267     176     127     118
                 114      44
 
MspA1I CmG'CkG

Cuts at:    306     927    3281    3595    3955    4968    5213     306 
   Size:        621    2354     314     360    1013     245    1263

  Fragments arranged by size: 

                2354    1263    1013     621     360     314     245
 
NaeI GCC'GGC

Cuts at:   4351    4351 
   Size:       6170
 
NarI GG'CG_CC

Cuts at:   3601    3849    3601 
   Size:        248    5922
 
NcoI C'CATG_G

Cuts at:   1869    2207    2325    2331    2672    1869 
   Size:        338     118       6     341    5367

  Fragments arranged by size: 

                5367     341     338     118       6
 
NdeI CA'TA_TG

Cuts at:    898     898 
   Size:       6170
 
NgoMIV G'CCGG_C

Cuts at:   4349    4349 
   Size:       6170
 
NsiI A_TGCA'T

Cuts at:    546     546 
   Size:       6170
 
NspI r_CATG'y

Cuts at:   2007    3223    3723    4630    2007 
   Size:       1216     500     907    3547

  Fragments arranged by size: 

                3547    1216     907     500
 
PasI CC'CwG_GG

Cuts at:   1762    1762 
   Size:       6170
 
PciI A'CATG_T

Cuts at:   2003    4626    2003 
   Size:       2623    3547
 
PflMI CCAn_nnn'nTGG

Cuts at:   1225    2191    1225 
   Size:        966    5204
 
PfoI T'CCnGG_A

Cuts at:    932    1280    2790     932 
   Size:        348    1510    4312

  Fragments arranged by size: 

                4312    1510     348
 
PmeI GTTT'AAAC

Cuts at:   3169    3169 
   Size:       6170
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1342    1374    5309    5341    1342 
   Size:         32    3935      32    2171

  Fragments arranged by size: 

                3935    2171      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    930    2691     930 
   Size:       1761    4409
 
PshAI GACnn'nnGTC

Cuts at:   1372    1372 
   Size:       6170
 
PsiI TTA'TAA

Cuts at:   1262    1262 
   Size:       6170
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1460    1492    1460 
   Size:         32    6138
 
PstI C_TGCA'G

Cuts at:   1019    2297    3005    3217    3902    1019 
   Size:       1278     708     212     685    3287

  Fragments arranged by size: 

                3287    1278     708     685     212
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6170
 
PvuII CAG'CTG

Cuts at:    306    3595    3955     306 
   Size:       3289     360    2521

  Fragments arranged by size: 

                3289    2521     360
 
RsrII CG'GwC_CG

Cuts at:   4365    4365 
   Size:       6170
 
SacI G_AGCT'C

Cuts at:   3184    3184 
   Size:       6170
 
SalI G'TCGA_C

Cuts at:    223    3207     223 
   Size:       2984    3186
 
SapI GCTCTTCn'nnn_

Cuts at:   2255    2299    4193    4403    2255 
   Size:         44    1894     210    4022

  Fragments arranged by size: 

                4022    1894     210      44
 
SbfI CC_TGCA'GG

Cuts at:   2297    3217    2297 
   Size:        920    5250
 
ScaI AGT'ACT

Cuts at:   5687    5687 
   Size:       6170
 
SfcI C'TryA_G

Cuts at:     33     567    1015    2293    3001    3213    3898    4891    5082 
   Size:        534     448    1278     708     212     685     993     191

Cuts at:   5082      33 
   Size:       1121

  Fragments arranged by size: 

                1278    1121     993     708     685     534     448     212
                 191
 
SfoI GGC'GCC

Cuts at:   3602    3850    3602 
   Size:        248    5922
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6170
 
SgrAI Cr'CCGG_yG

Cuts at:   2546    2546 
   Size:       6170
 
SmaI CCC'GGG

Cuts at:   3192    3192 
   Size:       6170
 
SmlI C'TyrA_G

Cuts at:   3325    4240    4732    4994    3325 
   Size:        915     492     262    4501

  Fragments arranged by size: 

                4501     915     492     262
 
SnaBI TAC'GTA

Cuts at:   3162    3162 
   Size:       6170
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6170
 
SphI G_CATG'C

Cuts at:   3223    3723    3223 
   Size:        500    5670
 
SspI AAT'ATT

Cuts at:   2133    2133 
   Size:       6170
 
StuI AGG'CCT

Cuts at:   1224    1224 
   Size:       6170
 
StyI C'CwwG_G

Cuts at:   1219    1869    2207    2325    2331    2672    3052    3304    3658 
   Size:        650     338     118       6     341     380     252     354

Cuts at:   3658    1219 
   Size:       3731

  Fragments arranged by size: 

                3731     650     380     354     341     338     252     118
                   6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    721     904    1353    2202    3753    3886    4345    4554     721 
   Size:        183     449     849    1551     133     459     209    2337

  Fragments arranged by size: 

                2337    1551     849     459     449     209     183     133
            
 
TatI w'GTAC_w

Cuts at:   5685    5685 
   Size:       6170
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2687    2719    2824    2856    2972    3004    5687    5719    2687 
   Size:         32     105      32     116      32    2683      32    3138

  Fragments arranged by size: 

                3138    2683     116     105      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   3075    3967    3075 
   Size:        892    5278
 
XbaI T'CTAG_A

Cuts at:   3201    3201 
   Size:       6170
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1181    1744    2625    3665    1181 
   Size:        563     881    1040    3686

  Fragments arranged by size: 

                3686    1040     881     563
 
XmaI C'CCGG_G

Cuts at:   3190    3190 
   Size:       6170
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI     AscI
     BaeI    BbvCI     BcgI     BmtI   Bpu10I    BsaXI    BseRI    BsiWI
    BsmBI    BsrGI   BssHII   BstEII   Bsu36I    CspCI   DraIII    EcoRV
     FseI    FspAI     MfeI     NheI     NotI     NruI     PacI     PmlI
   PspOMI    PspXI    SacII    SanDI    SexAI     SfiI     SrfI     SwaI
     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com