Restriction map for pF1KSDA1443
 (Circular) (Six-base) MAPSORT of: pF1KSDA1443.seq  Check: 3610  from: 1  to: 4784



pF1KSDA1443  4784 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    341    1596     341 
   Size:       1255    3529
 
AccI GT'mk_AC

Cuts at:    506    1822     506 
   Size:       1316    3468
 
Acc65I G'GTAC_C

Cuts at:   1800    1800 
   Size:       4784
 
AclI AA'CG_TT

Cuts at:   4624    4624 
   Size:       4784
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    194     330     494    2609    3041    3788    4056     194 
   Size:        136     164    2115     432     747     268     922

  Fragments arranged by size: 

                2115     922     747     432     268     164     136
 
AflIII A'CryG_T

Cuts at:   2136    3240    4217    2136 
   Size:       1104     977    2703

  Fragments arranged by size: 

                2703    1104     977
 
AgeI A'CCGG_T

Cuts at:   3172    3172 
   Size:       4784
 
AlwNI CAG_nnn'CTG

Cuts at:   1008    1399    1611    2089    3656    1008 
   Size:        391     212     478    1567    2136

  Fragments arranged by size: 

                2136    1567     478     391     212
 
ApaLI G'TGCA_C

Cuts at:   3554    3554 
   Size:       4784
 
ApoI r'AATT_y

Cuts at:   1788    4137    4393    1788 
   Size:       2349     256    2179

  Fragments arranged by size: 

                2349    2179     256
 
AseI AT'TA_AT

Cuts at:     20     928      20 
   Size:        908    3876
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4784
 
AvaI C'yCGr_G

Cuts at:    151     491     497     902    1804     151 
   Size:        340       6     405     902    3131

  Fragments arranged by size: 

                3131     902     405     340       6
 
BamHI G'GATC_C

Cuts at:   1809    1809 
   Size:       4784
 
BanI G'GyrC_C

Cuts at:    193     579    1402    1800    2214    2462    2497     193 
   Size:        386     823     398     414     248      35    2480

  Fragments arranged by size: 

                2480     823     414     398     386     248      35
 
BanII G_rGCy'C

Cuts at:    248     498     689    1798    2828     248 
   Size:        250     191    1109    1030    2204

  Fragments arranged by size: 

                2204    1109    1030     250     191
 
BbeI G_GCGC'C

Cuts at:   2218    2466    2218 
   Size:        248    4536
 
BbvCI CC'TCA_GC

Cuts at:   1637    1646    1637 
   Size:          9    4775
 
BciVI GTATCCnnnnn_n'

Cuts at:   2682    3443    4784    2682 
   Size:        761    1341    2682

  Fragments arranged by size: 

                2682    1341     761
 
BclI T'GATC_A

Cuts at:   1760    2304    1760 
   Size:        544    4240
 
BglI GCCn_nnn'nGGC

Cuts at:    153    1125     153 
   Size:        972    3812
 
BglII A'GATC_T

Cuts at:   2299    4336    2299 
   Size:       2037    2747
 
BlpI GC'TnA_GC

Cuts at:   1594    1896    1594 
   Size:        302    4482
 
Bme1580I G_kGCm'C

Cuts at:    182    1269    1407    2409    2502    3558     182 
   Size:       1087     138    1002      93    1056    1408

  Fragments arranged by size: 

                1408    1087    1056    1002     138      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1073    1542    1996    2077    2412    4556    1073 
   Size:        469     454      81     335    2144    1301

  Fragments arranged by size: 

                2144    1301     469     454     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    705    3151     705 
   Size:       2446    2338
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    575     587    1027     575 
   Size:         12     440    4332

  Fragments arranged by size: 

                4332     440      12
 
Bpu10I CC'TnA_GC

Cuts at:   1637    1646    1637 
   Size:          9    4775
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1960    2839    3331    3629    1960 
   Size:        879     492     298    3115

  Fragments arranged by size: 

                3115     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    229    4488     229 
   Size:       4259     525
 
BsaAI yAC'GTr

Cuts at:   1776    2137    1776 
   Size:        361    4423
 
BsaBI GATnn'nnATC

Cuts at:      6    2298    4335       6 
   Size:       2292    2037     455

  Fragments arranged by size: 

                2292    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2215    2463    3165    4661    2215 
   Size:        248     702    1496    2338

  Fragments arranged by size: 

                2338    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1506    1973    2494    3172    3446    3593    4004    1506 
   Size:        467     521     678     274     147     411    2286

  Fragments arranged by size: 

                2286     678     521     467     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    186     216     186 
   Size:         30    4754
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    286     575     578     589     697     964    1696    1699    1732 
   Size:        289       3      11     108     267     732       3      33

Cuts at:   1732    1735    1738    1741     286 
   Size:          3       3       3    3329

  Fragments arranged by size: 

                3329     732     289     267     108      33      11       3
                   3       3       3       3
 
BseYI C'CCAG_C

Cuts at:    137     364     440     890    1422    1601    2209    2967    3544 
   Size:        227      76     450     532     179     608     758     577

Cuts at:   3544    4040     137 
   Size:        496     881

  Fragments arranged by size: 

                 881     758     608     577     532     496     450     227
                 179      76
 
BsiEI CG_ry'CG

Cuts at:    104    2326    2372    3580     104 
   Size:       2222      46    1208    1308

  Fragments arranged by size: 

                2222    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1798    2576    2766    3558    1798 
   Size:        778     190     792    3024

  Fragments arranged by size: 

                3024     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    760     760 
   Size:       4784
 
Bsp1286I G_dGCh'C

Cuts at:    182     248     498     689    1269    1407    1798    2409    2502 
   Size:         66     250     191     580     138     391     611      93

Cuts at:   2502    2576    2766    2828    3558     182 
   Size:         74     190      62     730    1408

  Fragments arranged by size: 

                1408     730     611     580     391     250     191     190
                 138      93      74      66      62
 
BspEI T'CCGG_A

Cuts at:   4004    4004 
   Size:       4784
 
BspHI T'CATG_A

Cuts at:   3960    3960 
   Size:       4784
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    341    1596    1834    2048    2350    2731     341 
   Size:       1255     238     214     302     381    2394

  Fragments arranged by size: 

                2394    1255     381     302     238     214
 
BsrBI CCG'CTC

Cuts at:     50    1454    3076    3130    4614    4779      50 
   Size:       1404    1622      54    1484     165      55

  Fragments arranged by size: 

                1622    1484    1404     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    330     377    2696     330 
   Size:         47    2319    2418

  Fragments arranged by size: 

                2418    2319      47
 
BsrFI r'CCGG_y

Cuts at:   2782    2963    3172    2782 
   Size:        181     209    4394

  Fragments arranged by size: 

                4394     209     181
 
BssSI C'ACGA_G

Cuts at:   3055    3413    3055 
   Size:        358    4426
 
BstAPI GCAn_nnn'nTGC

Cuts at:    379     931    2161     379 
   Size:        552    1230    3002

  Fragments arranged by size: 

                3002    1230     552
 
BstBI TT'CG_AA

Cuts at:    110    3145     110 
   Size:       3035    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:    847     847 
   Size:       4784
 
BstYI r'GATC_y

Cuts at:      1    1809    2276    2299    2634    2880    3892    3978    3990 
   Size:       1808     467      23     335     246    1012      86      12

Cuts at:   3990    4336       1 
   Size:        346     449

  Fragments arranged by size: 

                1808    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    680     680 
   Size:       4784
 
BtgI C'CryG_G

Cuts at:    125     125 
   Size:       4784
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2734    2915    3078    2734 
   Size:        181     163    4440

  Fragments arranged by size: 

                4440     181     163
 
BtsI GCAGTG_nn'

Cuts at:    703     759     807    2027     703 
   Size:         56      48    1220    3460

  Fragments arranged by size: 

                3460    1220      56      48
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1357    1392    1357 
   Size:         35    4749
 
DraI TTT'AAA

Cuts at:   1783    1783 
   Size:       4784
 
DraIII CAC_nnn'GTG

Cuts at:   1081    1273    1081 
   Size:        192    4592
 
DrdI GACnn_nn'nnGTC

Cuts at:   2490    3348    2490 
   Size:        858    3926
 
EaeI y'GGCC_r

Cuts at:   2369    2543    2934    2961    4306    2369 
   Size:        174     391      27    1345    2847

  Fragments arranged by size: 

                2847    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2369    2369 
   Size:       4784
 
EarI CTCTTCn'nnn_

Cuts at:    479     981     996    1667    1673    1709    2807    3017     479 
   Size:        502      15     671       6      36    1098     210    2246

  Fragments arranged by size: 

                2246    1098     671     502     210      36      15       6
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2992    3302    3448    4594    2992 
   Size:        310     146    1146    3182

  Fragments arranged by size: 

                3182    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1796    1796 
   Size:       4784
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    194     330     494     575     587    1027    2609    3041    3788 
   Size:        136     164      81      12     440    1582     432     747

Cuts at:   3788    4056     194 
   Size:        268     922

  Fragments arranged by size: 

                1582     922     747     440     432     268     164     136
                  81      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:    811    1524     811 
   Size:        713    4071
 
EcoO109I rG'GnC_Cy

Cuts at:    628     832    1923     628 
   Size:        204    1091    3489

  Fragments arranged by size: 

                3489    1091     204
 
EcoRI G'AATT_C

Cuts at:   1788    1788 
   Size:       4784
 
EcoRV GAT'ATC

Cuts at:   1566    1566 
   Size:       4784
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2803    2803 
   Size:       4784
 
FspI TGC'GCA

Cuts at:   2565    2565 
   Size:       4784
 
HaeII r_GCGC'y

Cuts at:    447    2218    2466    3488    4467     447 
   Size:       1771     248    1022     979     764

  Fragments arranged by size: 

                1771    1022     979     764     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    943     975    1467    1499    2626    2656    2658    2688     943 
   Size:         32     492      32    1127      30       2      30    3039

  Fragments arranged by size: 

                3039    1127     492      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1823    1823 
   Size:       4784
 
KasI G'GCGC_C

Cuts at:   2214    2462    2214 
   Size:        248    4536
 
KpnI G_GTAC'C

Cuts at:   1804    1804 
   Size:       4784
 
MfeI C'AATT_G

Cuts at:    920    1573     920 
   Size:        653    4131
 
MluI A'CGCG_T

Cuts at:   4217    4217 
   Size:       4784
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    658    3455    3639     658 
   Size:       2797     184    1803

  Fragments arranged by size: 

                2797    1803     184
 
MscI TGG'CCA

Cuts at:   2545    2545 
   Size:       4784
 
MslI CAynn'nnrTG

Cuts at:   2773    2900    2773 
   Size:        127    4657
 
MspA1I CmG'CkG

Cuts at:    221     241     452    1227    1645    1895    2209    2569    3582 
   Size:         20     211     775     418     250     314     360    1013

Cuts at:   3582    3827     221 
   Size:        245    1178

  Fragments arranged by size: 

                1178    1013     775     418     360     314     250     245
                 211      20
 
NaeI GCC'GGC

Cuts at:   2965    2965 
   Size:       4784
 
NarI GG'CG_CC

Cuts at:   2215    2463    2215 
   Size:        248    4536
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       4784
 
NgoMIV G'CCGG_C

Cuts at:   2963    2963 
   Size:       4784
 
NspI r_CATG'y

Cuts at:   1837    2337    3244    1837 
   Size:        500     907    3377

  Fragments arranged by size: 

                3377     907     500
 
PciI A'CATG_T

Cuts at:   3240    3240 
   Size:       4784
 
PflMI CCAn_nnn'nTGG

Cuts at:   1008    1008 
   Size:       4784
 
PfoI T'CCnGG_A

Cuts at:    865     865 
   Size:       4784
 
PmeI GTTT'AAAC

Cuts at:   1783    1783 
   Size:       4784
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    320     352    3923    3955     320 
   Size:         32    3571      32    1149

  Fragments arranged by size: 

                3571    1149      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    628     832     628 
   Size:        204    4580
 
PspXI vC'TCGA_Gb

Cuts at:    491     491 
   Size:       4784
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1076    1108    1076 
   Size:         32    4752
 
PstI C_TGCA'G

Cuts at:   1831    2516    1831 
   Size:        685    4099
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4784
 
PvuII CAG'CTG

Cuts at:   1227    1645    2209    2569    1227 
   Size:        418     564     360    3442

  Fragments arranged by size: 

                3442     564     418     360
 
RsrII CG'GwC_CG

Cuts at:   2979    2979 
   Size:       4784
 
SacI G_AGCT'C

Cuts at:   1798    1798 
   Size:       4784
 
SalI G'TCGA_C

Cuts at:   1821    1821 
   Size:       4784
 
SanDI GG'GwC_CC

Cuts at:    832     832 
   Size:       4784
 
SapI GCTCTTCn'nnn_

Cuts at:   2807    3017    2807 
   Size:        210    4574
 
SbfI CC_TGCA'GG

Cuts at:   1831    1831 
   Size:       4784
 
ScaI AGT'ACT

Cuts at:   4301    4301 
   Size:       4784
 
SexAI A'CCwGG_T

Cuts at:   1146    1398    1146 
   Size:        252    4532
 
SfcI C'TryA_G

Cuts at:     33     277    1827    2512    3505    3696      33 
   Size:        244    1550     685     993     191    1121

  Fragments arranged by size: 

                1550    1121     993     685     244     191
 
SfoI GGC'GCC

Cuts at:   2216    2464    2216 
   Size:        248    4536
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4784
 
SmaI CCC'GGG

Cuts at:    153    1806     153 
   Size:       1653    3131
 
SmlI C'TyrA_G

Cuts at:    491    1939    2854    3346    3608     491 
   Size:       1448     915     492     262    1667

  Fragments arranged by size: 

                1667    1448     915     492     262
 
SnaBI TAC'GTA

Cuts at:   1776    1776 
   Size:       4784
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4784
 
SphI G_CATG'C

Cuts at:   1837    2337    1837 
   Size:        500    4284
 
SrfI GCCC'GGGC

Cuts at:    153     153 
   Size:       4784
 
StyI C'CwwG_G

Cuts at:    125    1122    1520    1918    2272     125 
   Size:        997     398     398     354    2637

  Fragments arranged by size: 

                2637     997     398     398     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1092    1615    2367    2500    2959    3168    1092 
   Size:        523     752     133     459     209    2708

  Fragments arranged by size: 

                2708     752     523     459     209     133
 
TatI w'GTAC_w

Cuts at:   4299    4299 
   Size:       4784
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    186     218    4301    4333     186 
   Size:         32    4083      32     637

  Fragments arranged by size: 

                4083     637      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2581    2581 
   Size:       4784
 
XbaI T'CTAG_A

Cuts at:   1662    1815    1662 
   Size:        153    4631
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1224    2279    1224 
   Size:       1055    3729
 
XhoI C'TCGA_G

Cuts at:    491     491 
   Size:       4784
 
XmaI C'CCGG_G

Cuts at:    151    1804     151 
   Size:       1653    3131
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI    BmgBI     BmtI
     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I     ClaI
     FseI    FspAI  HindIII     HpaI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PmlI    PshAI     PsiI   PspOMI    SacII
     SfiI    SgrAI     SspI     StuI     SwaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com