Restriction map for
pF1KSDA1410
(Circular) (Six-base) MAPSORT of: pF1KSDA1410.seq Check: 4664 from: 1 to: 15932
pF1KSDA1410 15932 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 642 1545 2492 4190 7731 12222 642
Size: 903 947 1698 3541 4491 4352
Fragments arranged by size:
4491 4352 3541 1698 947 903
AatII G_ACGT'C
Cuts at: 5767 12098 5767
Size: 6331 9601
AccI GT'mk_AC
Cuts at: 3634 8134 12970 3634
Size: 4500 4836 6596
Fragments arranged by size:
6596 4836 4500
Acc65I G'GTAC_C
Cuts at: 6696 12948 6696
Size: 6252 9680
AclI AA'CG_TT
Cuts at: 15772 15772
Size: 15932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 880 1039 1156 1175 3103 3117 3706 3826
Size: 308 159 117 19 1928 14 589 120
Cuts at: 3826 5236 6235 6890 7813 8395 9466 10066 10068
Size: 1410 999 655 923 582 1071 600 2
Cuts at: 10068 10306 10627 11527 13757 14189 14936 15204 572
Size: 238 321 900 2230 432 747 268 1300
Fragments arranged by size:
2230 1928 1410 1300 1071 999 923 900
747 655 600 589 582 432 321 308
268 238 159 120 117 19 14 2
AfeI AGC'GCT
Cuts at: 3922 4598 7609 9811 3922
Size: 676 3011 2202 10043
Fragments arranged by size:
10043 3011 2202 676
AflIII A'CryG_T
Cuts at: 1245 4244 5960 13284 14388 15365 1245
Size: 2999 1716 7324 1104 977 1812
Fragments arranged by size:
7324 2999 1812 1716 1104 977
AgeI A'CCGG_T
Cuts at: 14320 14320
Size: 15932
AhdI GACnn_n'nnGTC
Cuts at: 7680 9771 10821 7680
Size: 2091 1050 12791
Fragments arranged by size:
12791 2091 1050
AleI CACnn'nnGTG
Cuts at: 1624 2605 4168 9046 1624
Size: 981 1563 4878 8510
Fragments arranged by size:
8510 4878 1563 981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1858 5218 9094 11996 12533 1858
Size: 3360 3876 2902 537 5257
Fragments arranged by size:
5257 3876 3360 2902 537
AlwNI CAG_nnn'CTG
Cuts at: 379 669 723 747 1017 1356 2445 2574 2870
Size: 290 54 24 270 339 1089 129 296
Cuts at: 2870 3099 3217 3426 3609 3714 3903 6512 7407
Size: 229 118 209 183 105 189 2609 895
Cuts at: 7407 7518 7860 9471 10197 10284 10308 10998 11173
Size: 111 342 1611 726 87 24 690 175
Cuts at: 11173 12249 13237 14804 379
Size: 1076 988 1567 1507
Fragments arranged by size:
2609 1611 1567 1507 1089 1076 988 895
726 690 342 339 296 290 270 229
209 189 183 175 129 118 111 105
87 54 24 24
ApaI G_GGCC'C
Cuts at: 178 1380 3384 5714 7159 7332 9613 11257 11633
Size: 1202 2004 2330 1445 173 2281 1644 376
Cuts at: 11633 178
Size: 4477
Fragments arranged by size:
4477 2330 2281 2004 1644 1445 1202 376
173
ApaLI G'TGCA_C
Cuts at: 2134 3038 4351 7040 14702 2134
Size: 904 1313 2689 7662 3364
Fragments arranged by size:
7662 3364 2689 1313 904
ApoI r'AATT_y
Cuts at: 1747 2815 8781 10912 12513 12526 12936 15285 15541
Size: 1068 5966 2131 1601 13 410 2349 256
Cuts at: 15541 1747
Size: 2138
Fragments arranged by size:
5966 2349 2138 2131 1601 1068 410 256
13
AseI AT'TA_AT
Cuts at: 20 20
Size: 15932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 15932
AvaI C'yCGr_G
Cuts at: 237 954 1510 1716 5178 7161 8961 9090 10129
Size: 717 556 206 3462 1983 1800 129 1039
Cuts at: 10129 10572 11258 11871 12952 237
Size: 443 686 613 1081 3217
Fragments arranged by size:
3462 3217 1983 1800 1081 1039 717 686
613 556 443 206 129
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 703 736 4261 4294 6680 6713 7513 7546 8319
Size: 33 3525 33 2386 33 800 33 773
Cuts at: 8319 8352 703
Size: 33 8283
Fragments arranged by size:
8283 3525 2386 800 773 33 33 33
33 33
BamHI G'GATC_C
Cuts at: 1199 3446 9341 12434 12654 12957 1199
Size: 2247 5895 3093 220 303 4174
Fragments arranged by size:
5895 4174 3093 2247 303 220
BanI G'GyrC_C
Cuts at: 654 788 1191 1557 4445 4855 5145 6472 6610
Size: 134 403 366 2888 410 290 1327 138
Cuts at: 6610 6696 7111 9021 9040 10809 11328 12025 12120
Size: 86 415 1910 19 1769 519 697 95
Cuts at: 12120 12579 12643 12948 13362 13610 13645 654
Size: 459 64 305 414 248 35 2941
Fragments arranged by size:
2941 2888 1910 1769 1327 697 519 459
415 414 410 403 366 305 290 248
138 134 95 86 64 35 19
BanII G_rGCy'C
Cuts at: 178 644 1380 3384 3694 3872 4836 5714 6155
Size: 466 736 2004 310 178 964 878 441
Cuts at: 6155 6170 6440 7159 7332 7358 7715 8278 8301
Size: 15 270 719 173 26 357 563 23
Cuts at: 8301 9290 9613 9698 10783 10943 11257 11633 12347
Size: 989 323 85 1085 160 314 376 714
Cuts at: 12347 12946 13976 178
Size: 599 1030 2134
Fragments arranged by size:
2134 2004 1085 1030 989 964 878 736
719 714 599 563 466 441 376 357
323 314 310 270 178 173 160 85
26 23 15
BbeI G_GCGC'C
Cuts at: 13366 13614 13366
Size: 248 15684
BbsI GAAGACnn'nnnn_
Cuts at: 560 5339 6829 7455 8283 11298 11537 12450 560
Size: 4779 1490 626 828 3015 239 913 4042
Fragments arranged by size:
4779 4042 3015 1490 913 828 626 239
BbvCI CC'TCA_GC
Cuts at: 430 1220 1426 1663 2830 3572 4066 4693 6037
Size: 790 206 237 1167 742 494 627 1344
Cuts at: 6037 6193 6678 430
Size: 156 485 9684
Fragments arranged by size:
9684 1344 1167 790 742 627 494 485
237 206 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4176 4210 5025 5059 7572 7606 8923 8957 11239
Size: 34 815 34 2513 34 1317 34 2282
Cuts at: 11239 11273 4176
Size: 34 8835
Fragments arranged by size:
8835 2513 2282 1317 815 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8748 13830 14591 15932 8748
Size: 5082 761 1341 8748
Fragments arranged by size:
8748 5082 1341 761
BclI T'GATC_A
Cuts at: 3986 4790 5264 9149 12236 13452 3986
Size: 804 474 3885 3087 1216 6466
Fragments arranged by size:
6466 3885 3087 1216 804 474
BfrBI ATG'CAT
Cuts at: 9419 9419
Size: 15932
BglI GCCn_nnn'nGGC
Cuts at: 4378 4874 8827 9294 9482 4378
Size: 496 3953 467 188 10828
Fragments arranged by size:
10828 3953 496 467 188
BglII A'GATC_T
Cuts at: 2684 4139 5273 10133 11670 11960 13447 15484 2684
Size: 1455 1134 4860 1537 290 1487 2037 3132
Fragments arranged by size:
4860 3132 2037 1537 1487 1455 1134 290
BlpI GC'TnA_GC
Cuts at: 2720 3275 5359 10318 13044 2720
Size: 555 2084 4959 2726 5608
Fragments arranged by size:
5608 4959 2726 2084 555
Bme1580I G_kGCm'C
Cuts at: 178 1380 2138 2219 3042 3384 4355 4860 5498
Size: 1202 758 81 823 342 971 505 638
Cuts at: 5498 5714 6731 7044 7159 7332 9613 11257 11633
Size: 216 1017 313 115 173 2281 1644 376
Cuts at: 11633 12028 12648 12775 12912 13557 13650 14706 178
Size: 395 620 127 137 645 93 1056 1404
Fragments arranged by size:
2281 1644 1404 1202 1056 1017 971 823
758 645 638 620 505 395 376 342
313 216 173 137 127 115 93 81
BmgBI CAC'GTC
Cuts at: 4629 11841 4629
Size: 7212 8720
BmrI ACTGGGnnnn_n'
Cuts at: 1197 1896 3465 4398 6488 9069 10169 10207 11431
Size: 699 1569 933 2090 2581 1100 38 1224
Cuts at: 11431 11799 12157 13144 13225 13560 15704 1197
Size: 368 358 987 81 335 2144 1425
Fragments arranged by size:
2581 2144 2090 1569 1425 1224 1100 987
933 699 368 358 335 81 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1406 1536 6403 14299 1406
Size: 130 4867 7896 3039
Fragments arranged by size:
7896 4867 3039 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 1762 2173 2245 2293 2410 2788 3150 3304
Size: 1496 411 72 48 117 378 362 154
Cuts at: 3304 3547 3847 3886 4099 4252 4281 4504 6169
Size: 243 300 39 213 153 29 223 1665
Cuts at: 6169 6280 6483 6610 7048 7400 8614 8704 8725
Size: 111 203 127 438 352 1214 90 21
Cuts at: 8725 9047 9328 9460 9982 10015 10252 11089 11632
Size: 322 281 132 522 33 237 837 543
Cuts at: 11632 12322 12369 266
Size: 690 47 3829
Fragments arranged by size:
3829 1665 1496 1214 837 690 543 522
438 411 378 362 352 322 300 281
243 237 223 213 203 154 153 132
127 117 111 90 72 48 47 39
33 29 21
Bpu10I CC'TnA_GC
Cuts at: 430 724 1220 1357 1381 1426 1562 1663 2830
Size: 294 496 137 24 45 136 101 1167
Cuts at: 2830 3572 4066 4693 6037 6193 6517 6678 9664
Size: 742 494 627 1344 156 324 161 2986
Cuts at: 9664 11852 12125 12341 430
Size: 2188 273 216 4021
Fragments arranged by size:
4021 2986 2188 1344 1167 742 627 496
494 324 294 273 216 161 156 137
136 101 45 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1780 4828 5413 5503 5614 6325 6373 6508 6835
Size: 3048 585 90 111 711 48 135 327
Cuts at: 6835 7588 7999 9124 9994 10567 10957 11680 11725
Size: 753 411 1125 870 573 390 723 45
Cuts at: 11725 12100 12247 12256 12358 13108 13987 14479 14777
Size: 375 147 9 102 750 879 492 298
Cuts at: 14777 1780
Size: 2935
Fragments arranged by size:
3048 2935 1125 879 870 753 750 723
711 585 573 492 411 390 375 327
298 147 135 111 102 90 48 45
9
BsaI GGTCTCn'nnnn_
Cuts at: 938 1507 1873 4075 4885 5668 7024 8767 15636
Size: 569 366 2202 810 783 1356 1743 6869
Cuts at: 15636 938
Size: 1234
Fragments arranged by size:
6869 2202 1743 1356 1234 810 783 569
366
BsaAI yAC'GTr
Cuts at: 3037 5823 8941 12924 13285 3037
Size: 2786 3118 3983 361 5684
Fragments arranged by size:
5684 3983 3118 2786 361
BsaBI GATnn'nnATC
Cuts at: 6 697 3481 10093 11669 13446 15483 6
Size: 691 2784 6612 1576 1777 2037 455
Fragments arranged by size:
6612 2784 2037 1777 1576 691 455
BsaHI Gr'CG_yC
Cuts at: 1043 5764 7814 12095 13363 13611 14313 15809 1043
Size: 4721 2050 4281 1268 248 702 1496 1166
Fragments arranged by size:
4721 4281 2050 1496 1268 1166 702 248
BsaWI w'CCGG_w
Cuts at: 4828 10812 13121 13642 14320 14594 14741 15152 4828
Size: 5984 2309 521 678 274 147 411 5608
Fragments arranged by size:
5984 5608 2309 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 565 595 916 946 2398 2428 3148 3178 3451
Size: 30 321 30 1452 30 720 30 273
Cuts at: 3451 3481 3613 3643 4258 4288 6456 6486 9433
Size: 30 132 30 615 30 2168 30 2947
Cuts at: 9433 9463 9550 9580 10525 10555 11113 11143 11554
Size: 30 87 30 945 30 558 30 411
Cuts at: 11554 11584 11830 11860 12535 12565 565
Size: 30 246 30 675 30 3932
Fragments arranged by size:
3932 2947 2168 1452 945 720 675 615
558 411 321 273 246 132 87 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 720 1075 1561 1564 2674 2725 2785 2944 3406
Size: 355 486 3 1110 51 60 159 462
Cuts at: 3406 3844 4104 4279 5470 5581 5866 5898 6241
Size: 438 260 175 1191 111 285 32 343
Cuts at: 6241 6328 6649 6666 6838 7588 7591 7765 8722
Size: 87 321 17 172 750 3 174 957
Cuts at: 8722 8785 8812 9448 9991 10231 10249 10314 12094
Size: 63 27 636 543 240 18 65 1780
Cuts at: 12094 720
Size: 4558
Fragments arranged by size:
4558 1780 1191 1110 957 750 636 543
486 462 438 355 343 321 285 260
240 175 174 172 159 111 87 65
63 60 51 32 27 18 17 3
3
BseYI C'CCAG_C
Cuts at: 2219 2518 4441 4517 4860 4869 5002 5419 5443
Size: 299 1923 76 343 9 133 417 24
Cuts at: 5443 6154 7400 7860 7878 9471 9650 9714 9806
Size: 711 1246 460 18 1593 179 64 92
Cuts at: 9806 9987 10050 11422 12450 12650 12869 13357 14115
Size: 181 63 1372 1028 200 219 488 758
Cuts at: 14115 14692 15188 2219
Size: 577 496 2963
Fragments arranged by size:
2963 1923 1593 1372 1246 1028 758 711
577 496 488 460 417 343 299 219
200 181 179 133 92 76 64 63
24 18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 1804 1990 2016 2926 4075 4192 4822 6766
Size: 1600 186 26 910 1149 117 630 1944
Cuts at: 6766 7531 8809 204
Size: 765 1278 7327
Fragments arranged by size:
7327 1944 1600 1278 1149 910 765 630
186 117 26
BsiEI CG_ry'CG
Cuts at: 104 4169 7098 7899 9739 13474 13520 14728 104
Size: 4065 2929 801 1840 3735 46 1208 1308
Fragments arranged by size:
4065 3735 2929 1840 1308 1208 801 46
BsiHKAI G_wGCw'C
Cuts at: 195 1387 1424 2027 2138 2477 3042 4355 4836
Size: 1192 37 603 111 339 565 1313 481
Cuts at: 4836 5828 7044 7715 8301 9641 11369 11846 11858
Size: 992 1216 671 586 1340 1728 477 12
Cuts at: 11858 12347 12946 13724 13914 14706 195
Size: 489 599 778 190 792 1421
Fragments arranged by size:
1728 1421 1340 1313 1216 1192 992 792
778 671 603 599 586 565 489 481
477 339 190 111 37 12
BsmI GAATG_Cn'
Cuts at: 1143 3969 4016 4743 9421 1143
Size: 2826 47 727 4678 7654
Fragments arranged by size:
7654 4678 2826 727 47
BsmBI CGTCTCn'nnnn_
Cuts at: 9523 12001 9523
Size: 2478 13454
Bsp1286I G_dGCh'C
Cuts at: 178 195 644 1380 1387 1424 2027 2138 2219
Size: 17 449 736 7 37 603 111 81
Cuts at: 2219 2477 3042 3384 3694 3872 4355 4836 4860
Size: 258 565 342 310 178 483 481 24
Cuts at: 4860 5498 5714 5828 6155 6170 6440 6731 7044
Size: 638 216 114 327 15 270 291 313
Cuts at: 7044 7159 7332 7358 7715 8278 8301 9290 9613
Size: 115 173 26 357 563 23 989 323
Cuts at: 9613 9641 9698 10783 10943 11257 11369 11633 11846
Size: 28 57 1085 160 314 112 264 213
Cuts at: 11846 11858 12028 12347 12648 12775 12912 12946 13557
Size: 12 170 319 301 127 137 34 611
Cuts at: 13557 13650 13724 13914 13976 14706 178
Size: 93 74 190 62 730 1404
Fragments arranged by size:
1404 1085 989 736 730 638 611 603
565 563 483 481 449 357 342 327
323 319 314 313 310 301 291 270
264 258 216 213 190 178 173 170
160 137 127 115 114 112 111 93
81 74 62 57 37 34 28 26
24 23 17 15 12 7
BspEI T'CCGG_A
Cuts at: 15152 15152
Size: 15932
BspHI T'CATG_A
Cuts at: 2289 3731 3956 10724 10793 11807 15108 2289
Size: 1442 225 6768 69 1014 3301 3113
Fragments arranged by size:
6768 3301 3113 1442 1014 225 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 642 1545 2492 2639 3545 4190 6639 7731 9551
Size: 903 947 147 906 645 2449 1092 1820
Cuts at: 9551 10871 11510 12222 12410 12982 13196 13498 13879
Size: 1320 639 712 188 572 214 302 381
Cuts at: 13879 642
Size: 2695
Fragments arranged by size:
2695 2449 1820 1320 1092 947 906 903
712 645 639 572 381 302 214 188
147
BsrBI CCG'CTC
Cuts at: 50 2775 3939 8627 14224 14278 15762 15927 50
Size: 2725 1164 4688 5597 54 1484 165 55
Fragments arranged by size:
5597 4688 2725 1484 1164 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2321 2408 6077 7337 7872 9200 12148 12839 13844
Size: 87 3669 1260 535 1328 2948 691 1005
Cuts at: 13844 2321
Size: 4409
Fragments arranged by size:
4409 3669 2948 1328 1260 1005 691 535
87
BsrFI r'CCGG_y
Cuts at: 4489 5973 7020 8361 10313 11795 13930 14111 14320
Size: 1484 1047 1341 1952 1482 2135 181 209
Cuts at: 14320 4489
Size: 6101
Fragments arranged by size:
6101 2135 1952 1484 1482 1341 1047 209
181
BsrGI T'GTAC_A
Cuts at: 8462 10091 12323 12698 8462
Size: 1629 2232 375 11696
Fragments arranged by size:
11696 2232 1629 375
BssHII G'CGCG_C
Cuts at: 9246 9246
Size: 15932
BssSI C'ACGA_G
Cuts at: 3043 7534 12794 14203 14561 3043
Size: 4491 5260 1409 358 4414
Fragments arranged by size:
5260 4491 4414 1409 358
BstAPI GCAn_nnn'nTGC
Cuts at: 7518 8700 9303 9552 10664 13309 7518
Size: 1182 603 249 1112 2645 10141
Fragments arranged by size:
10141 2645 1182 1112 603 249
BstBI TT'CG_AA
Cuts at: 110 14293 110
Size: 14183 1749
BstEII G'GTnAC_C
Cuts at: 1146 1692 2260 4431 7629 11031 1146
Size: 546 568 2171 3198 3402 6047
Fragments arranged by size:
6047 3402 3198 2171 568 546
BstXI CCAn_nnnn'nTGG
Cuts at: 792 3024 4305 4470 4629 4905 5313 5847 6162
Size: 2232 1281 165 159 276 408 534 315
Cuts at: 6162 7269 7377 8220 9282 11067 11238 792
Size: 1107 108 843 1062 1785 171 5486
Fragments arranged by size:
5486 2232 1785 1281 1107 1062 843 534
408 315 276 171 165 159 108
BstYI r'GATC_y
Cuts at: 1 1199 1757 2498 2684 2882 3446 3941 4139
Size: 1198 558 741 186 198 564 495 198
Cuts at: 4139 4682 5135 5273 8405 9341 9731 10133 10304
Size: 543 453 138 3132 936 390 402 171
Cuts at: 10304 10397 10487 11670 11888 11960 12434 12654 12851
Size: 93 90 1183 218 72 474 220 197
Cuts at: 12851 12957 13424 13447 13782 14028 15040 15126 15138
Size: 106 467 23 335 246 1012 86 12
Cuts at: 15138 15484 1
Size: 346 449
Fragments arranged by size:
3132 1198 1183 1012 936 741 564 558
543 495 474 467 453 449 402 390
346 335 246 220 218 198 198 197
186 171 138 106 93 90 86 72
23 12
BstZ17I GTA'TAC
Cuts at: 8135 8135
Size: 15932
Bsu36I CC'TnA_GG
Cuts at: 170 7444 170
Size: 7274 8658
BtgI C'CryG_G
Cuts at: 125 355 1195 2279 3932 4163 4463 4970 5234
Size: 230 840 1084 1653 231 300 507 264
Cuts at: 5234 5678 6692 6734 7080 7151 7189 8735 9281
Size: 444 1014 42 346 71 38 1546 546
Cuts at: 9281 10469 11598 11910 12624 125
Size: 1188 1129 312 714 3433
Fragments arranged by size:
3433 1653 1546 1188 1129 1084 1014 840
714 546 507 444 346 312 300 264
231 230 71 42 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4188 4422 8748 9372 9587 10563 12353 13882 14063
Size: 234 4326 624 215 976 1790 1529 181
Cuts at: 14063 14226 4188
Size: 163 5894
Fragments arranged by size:
5894 4326 1790 1529 976 624 234 215
181 163
BtsI GCAGTG_nn'
Cuts at: 195 586 625 1285 1378 1954 2353 4592 5165
Size: 391 39 660 93 576 399 2239 573
Cuts at: 5165 6495 7627 9463 12247 13175 195
Size: 1330 1132 1836 2784 928 2952
Fragments arranged by size:
2952 2784 2239 1836 1330 1132 928 660
576 573 399 391 93 39
ClaI AT'CG_AT
Cuts at: 3269 7004 9173 3269
Size: 3735 2169 10028
Fragments arranged by size:
10028 3735 2169
DraI TTT'AAA
Cuts at: 12931 12931
Size: 15932
DraIII CAC_nnn'GTG
Cuts at: 1246 8262 9270 11488 11556 1246
Size: 7016 1008 2218 68 5622
Fragments arranged by size:
7016 5622 2218 1008 68
DrdI GACnn_nn'nnGTC
Cuts at: 1495 1701 2803 3213 3727 8158 9652 13638 14496
Size: 206 1102 410 514 4431 1494 3986 858
Cuts at: 14496 1495
Size: 2931
Fragments arranged by size:
4431 3986 2931 1494 1102 858 514 410
206
EaeI y'GGCC_r
Cuts at: 1329 1642 1826 2201 2597 3101 4166 4425 4919
Size: 313 184 375 396 504 1065 259 494
Cuts at: 4919 4967 4994 5190 5237 6492 7490 7784 7896
Size: 48 27 196 47 1255 998 294 112
Cuts at: 7896 7931 8359 8384 8759 8897 8953 9272 9584
Size: 35 428 25 375 138 56 319 312
Cuts at: 9584 10472 10514 11057 12152 12710 13517 13691 14082
Size: 888 42 543 1095 558 807 174 391
Cuts at: 14082 14109 15454 1329
Size: 27 1345 1807
Fragments arranged by size:
1807 1345 1255 1095 1065 998 888 807
558 543 504 494 428 396 391 375
375 319 313 312 294 259 196 184
174 138 112 56 48 47 42 35
27 27 25
EagI C'GGCC_G
Cuts at: 4166 7896 13517 4166
Size: 3730 5621 6581
Fragments arranged by size:
6581 5621 3730
EarI CTCTTCn'nnn_
Cuts at: 1216 1506 2188 2300 3682 5230 5530 6557 6626
Size: 290 682 112 1382 1548 300 1027 69
Cuts at: 6626 7810 8056 8617 9118 9176 10498 10890 13955
Size: 1184 246 561 501 58 1322 392 3065
Cuts at: 13955 14165 1216
Size: 210 2983
Fragments arranged by size:
3065 2983 1548 1382 1322 1184 1027 682
561 501 392 300 290 246 210 112
69 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6969 11389 14140 14450 14596 15742 6969
Size: 4420 2751 310 146 1146 7159
Fragments arranged by size:
7159 4420 2751 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4834 7713 8299 12345 12944 4834
Size: 2879 586 4046 599 7822
Fragments arranged by size:
7822 4046 2879 599 586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 572 880 1039 1156 1175 1762 2173 2245
Size: 306 308 159 117 19 587 411 72
Cuts at: 2245 2293 2410 2788 3103 3117 3150 3304 3547
Size: 48 117 378 315 14 33 154 243
Cuts at: 3547 3706 3826 3847 3886 4099 4252 4281 4504
Size: 159 120 21 39 213 153 29 223
Cuts at: 4504 5236 6169 6235 6280 6483 6610 6890 7048
Size: 732 933 66 45 203 127 280 158
Cuts at: 7048 7400 7813 8395 8614 8704 8725 9047 9328
Size: 352 413 582 219 90 21 322 281
Cuts at: 9328 9460 9466 9982 10015 10066 10068 10252 10306
Size: 132 6 516 33 51 2 184 54
Cuts at: 10306 10627 11089 11527 11632 12322 12369 13757 14189
Size: 321 462 438 105 690 47 1388 432
Cuts at: 14189 14936 15204 266
Size: 747 268 994
Fragments arranged by size:
1388 994 933 747 732 690 587 582
516 462 438 432 413 411 378 352
322 321 315 308 306 281 280 268
243 223 219 213 203 184 159 159
158 154 153 132 127 120 117 117
105 90 72 66 54 51 48 47
45 39 33 33 29 21 21 19
14 6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 499 1565 1666 1969 2635 4909 5509 5593 7456
Size: 1066 101 303 666 2274 600 84 1863
Cuts at: 7456 7852 8014 8704 8866 9307 9349 9370 10234
Size: 396 162 690 162 441 42 21 864
Cuts at: 10234 11242 12262 499
Size: 1008 1020 4169
Fragments arranged by size:
4169 2274 1863 1066 1020 1008 864 690
666 600 441 396 303 162 162 101
84 42 21
EcoO109I rG'GnC_Cy
Cuts at: 164 174 212 222 999 1224 1377 1405 2081
Size: 10 38 10 777 225 153 28 676
Cuts at: 2081 2442 2592 4062 4158 4227 4379 4400 4989
Size: 361 150 1470 96 69 152 21 589
Cuts at: 4989 5006 5514 6240 6384 7156 7328 7329 7449
Size: 17 508 726 144 772 172 1 120
Cuts at: 7449 8007 8193 8679 8859 9087 9610 11253 11254
Size: 558 186 486 180 228 523 1643 1
Cuts at: 11254 11630 11736 13071 164
Size: 376 106 1335 3025
Fragments arranged by size:
3025 1643 1470 1335 777 772 726 676
589 558 523 508 486 376 361 228
225 186 180 172 153 152 150 144
120 106 96 69 38 28 21 17
10 10 1 1
EcoRI G'AATT_C
Cuts at: 2815 8781 10912 12936 2815
Size: 5966 2131 2024 5811
Fragments arranged by size:
5966 5811 2131 2024
EcoRV GAT'ATC
Cuts at: 8094 8094
Size: 15932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1500 1611 1632 3786 5241 6261 8562 9282 13951
Size: 111 21 2154 1455 1020 2301 720 4669
Cuts at: 13951 1500
Size: 3481
Fragments arranged by size:
4669 3481 2301 2154 1455 1020 720 111
21
FspI TGC'GCA
Cuts at: 1270 1399 1834 1915 3430 4186 5157 8245 8647
Size: 129 435 81 1515 756 971 3088 402
Cuts at: 8647 8908 9919 11278 11677 13713 1270
Size: 261 1011 1359 399 2036 3489
Fragments arranged by size:
3489 3088 2036 1515 1359 1011 971 756
435 402 399 261 129 81
HaeII r_GCGC'y
Cuts at: 3924 4097 4600 5224 7611 7861 9366 9813 11619
Size: 173 503 624 2387 250 1505 447 1806
Cuts at: 11619 11802 11870 13366 13614 14636 15615 3924
Size: 183 68 1496 248 1022 979 4241
Fragments arranged by size:
4241 2387 1806 1505 1496 1022 979 624
503 447 250 248 183 173 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 893 916 925 948 2398 2430 2943 2975 3148
Size: 23 9 23 1450 32 513 32 173
Cuts at: 3148 3180 3244 3276 3451 3483 3501 3533 3688
Size: 32 64 32 175 32 18 32 155
Cuts at: 3688 3720 4129 4161 4818 4850 5038 5070 5681
Size: 32 409 32 657 32 188 32 611
Cuts at: 5681 5713 7666 7687 7698 7719 9757 9789 10788
Size: 32 1953 21 11 21 2038 32 999
Cuts at: 10788 10807 10820 10839 11085 11113 11117 11145 11554
Size: 19 13 19 246 28 4 28 409
Cuts at: 11554 11586 11830 11862 12279 12311 12535 12567 12568
Size: 32 244 32 417 32 224 32 1
Cuts at: 12568 12600 12777 12809 13774 13804 13806 13836 893
Size: 32 177 32 965 30 2 30 2989
Fragments arranged by size:
2989 2038 1953 1450 999 965 657 611
513 417 409 409 246 244 224 188
177 175 173 155 64 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 30
30 28 28 23 23 21 21 19
19 18 13 11 9 4 2 1
HincII GTy'rAC
Cuts at: 3635 5814 8328 12593 12971 3635
Size: 2179 2514 4265 378 6596
Fragments arranged by size:
6596 4265 2514 2179 378
HindIII A'AGCT_T
Cuts at: 537 537
Size: 15932
HpaI GTT'AAC
Cuts at: 12593 12593
Size: 15932
KasI G'GCGC_C
Cuts at: 13362 13610 13362
Size: 248 15684
KpnI G_GTAC'C
Cuts at: 6700 12952 6700
Size: 6252 9680
MluI A'CGCG_T
Cuts at: 15365 15365
Size: 15932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 446 513 794 3210 3215 3494 4025 5253 5567
Size: 67 281 2416 5 279 531 1228 314
Cuts at: 5567 5963 7262 9485 10532 10646 10913 11150 14603
Size: 396 1299 2223 1047 114 267 237 3453
Cuts at: 14603 14787 446
Size: 184 1591
Fragments arranged by size:
3453 2416 2223 1591 1299 1228 1047 531
396 314 281 279 267 237 184 114
67 5
MscI TGG'CCA
Cuts at: 1828 2203 2599 3103 4427 4921 4969 4996 5239
Size: 375 396 504 1324 494 48 27 243
Cuts at: 5239 8386 8899 9274 10474 10516 11059 12154 13693
Size: 3147 513 375 1200 42 543 1095 1539
Cuts at: 13693 1828
Size: 4067
Fragments arranged by size:
4067 3147 1539 1324 1200 1095 543 513
504 494 396 375 375 243 48 42
27
MslI CAynn'nnrTG
Cuts at: 1624 2605 3022 3730 4168 4243 4468 4969 5047
Size: 981 417 708 438 75 225 501 78
Cuts at: 5047 5845 6724 7375 7378 7477 8257 8525 9046
Size: 798 879 651 3 99 780 268 521
Cuts at: 9046 10363 10468 10726 11149 11978 12706 12844 13921
Size: 1317 105 258 423 829 728 138 1077
Cuts at: 13921 14048 1624
Size: 127 3508
Fragments arranged by size:
3508 1317 1077 981 879 829 798 780
728 708 651 521 501 438 423 417
268 258 225 138 127 105 99 78
75 3
MspA1I CmG'CkG
Cuts at: 396 1805 2223 2258 2997 3081 3099 3282 3711
Size: 1409 418 35 739 84 18 183 429
Cuts at: 3711 3849 3903 4581 4692 4864 4873 5103 5755
Size: 138 54 678 111 172 9 230 652
Cuts at: 5755 5856 6131 6543 7191 7404 7515 7586 7895
Size: 101 275 412 648 213 111 71 309
Cuts at: 7895 8952 9468 9589 9650 10251 11012 12039 12300
Size: 1057 516 121 61 601 761 1027 261
Cuts at: 12300 12454 12650 12672 13043 13357 13717 14730 14975
Size: 154 196 22 371 314 360 1013 245
Cuts at: 14975 396
Size: 1353
Fragments arranged by size:
1409 1353 1057 1027 1013 761 739 678
652 648 601 516 429 418 412 371
360 314 309 275 261 245 230 213
196 183 172 154 138 121 111 111
101 84 71 61 54 35 22 18
9
NaeI GCC'GGC
Cuts at: 4491 7022 8363 14113 4491
Size: 2531 1341 5750 6310
Fragments arranged by size:
6310 5750 2531 1341
NarI GG'CG_CC
Cuts at: 13363 13611 13363
Size: 248 15684
NcoI C'CATG_G
Cuts at: 125 3932 4463 4970 6692 6734 7151 8735 11598
Size: 3807 531 507 1722 42 417 1584 2863
Cuts at: 11598 12624 125
Size: 1026 3433
Fragments arranged by size:
3807 3433 2863 1722 1584 1026 531 507
417 42
NgoMIV G'CCGG_C
Cuts at: 4489 7020 8361 14111 4489
Size: 2531 1341 5750 6310
Fragments arranged by size:
6310 5750 2531 1341
NotI GC'GGCC_GC
Cuts at: 7896 7896
Size: 15932
NsiI A_TGCA'T
Cuts at: 9421 9421
Size: 15932
NspI r_CATG'y
Cuts at: 1236 1320 1339 1944 2920 3348 3444 4248 4578
Size: 84 19 605 976 428 96 804 330
Cuts at: 4578 5802 5964 6771 7020 7476 7980 9555 12985
Size: 1224 162 807 249 456 504 1575 3430
Cuts at: 12985 13485 14392 1236
Size: 500 907 2776
Fragments arranged by size:
3430 2776 1575 1224 976 907 807 804
605 504 500 456 428 330 249 162
96 84 19
PacI TTA_AT'TAA
Cuts at: 2066 2066
Size: 15932
PasI CC'CwG_GG
Cuts at: 11238 11634 11238
Size: 396 15536
PciI A'CATG_T
Cuts at: 4244 5960 14388 4244
Size: 1716 8428 5788
Fragments arranged by size:
8428 5788 1716
PflMI CCAn_nnn'nTGG
Cuts at: 905 2808 2870 2955 3099 3363 3933 6762 7074
Size: 1903 62 85 144 264 570 2829 312
Cuts at: 7074 7860 8345 9270 9851 10197 10209 905
Size: 786 485 925 581 346 12 6628
Fragments arranged by size:
6628 2829 1903 925 786 581 570 485
346 312 264 144 85 62 12
PfoI T'CCnGG_A
Cuts at: 252 560 1046 2501 2747 3166 4220 4646 5588
Size: 308 486 1455 246 419 1054 426 942
Cuts at: 5588 6257 7553 8084 8183 9344 9959 11705 12635
Size: 669 1296 531 99 1161 615 1746 930
Cuts at: 12635 252
Size: 3549
Fragments arranged by size:
3549 1746 1455 1296 1161 1054 942 930
669 615 531 486 426 419 308 246
99
PmeI GTTT'AAAC
Cuts at: 12931 12931
Size: 15932
PmlI CAC'GTG
Cuts at: 3037 3037
Size: 15932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2952 6427 6459 15071 15103 2920
Size: 32 3475 32 8612 32 3749
Fragments arranged by size:
8612 3749 3475 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 164 212 999 1224 4158 4227 4400 5514 7449
Size: 48 787 225 2934 69 173 1114 1935
Cuts at: 7449 8007 8679 8859 9087 11736 164
Size: 558 672 180 228 2649 4360
Fragments arranged by size:
4360 2934 2649 1935 1114 787 672 558
228 225 180 173 69 48
PspOMI G'GGCC_C
Cuts at: 174 1376 3380 5710 7155 7328 9609 11253 11629
Size: 1202 2004 2330 1445 173 2281 1644 376
Cuts at: 11629 174
Size: 4477
Fragments arranged by size:
4477 2330 2281 2004 1644 1445 1202 376
173
PspXI vC'TCGA_Gb
Cuts at: 1716 1716
Size: 15932
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5167 5199 9532 9564 5167
Size: 32 4333 32 11535
Fragments arranged by size:
11535 4333 32 32
PstI C_TGCA'G
Cuts at: 201 632 1949 2360 2996 3542 5510 5617 9086
Size: 431 1317 411 636 546 1968 107 3469
Cuts at: 9086 9548 11453 12063 12233 12428 12512 12979 13664
Size: 462 1905 610 170 195 84 467 685
Cuts at: 13664 201
Size: 2469
Fragments arranged by size:
3469 2469 1968 1905 1317 685 636 610
546 467 462 431 411 195 170 107
84
PvuI CG_AT'CG
Cuts at: 104 9739 104
Size: 9635 6297
PvuII CAG'CTG
Cuts at: 1805 2223 2997 3081 3099 3282 3711 3903 4692
Size: 418 774 84 18 183 429 192 789
Cuts at: 4692 4864 4873 5755 5856 6543 7404 7515 7586
Size: 172 9 882 101 687 861 111 71
Cuts at: 7586 9650 10251 12039 12300 12454 12672 13357 13717
Size: 2064 601 1788 261 154 218 685 360
Cuts at: 13717 1805
Size: 4020
Fragments arranged by size:
4020 2064 1788 882 861 789 774 687
685 601 429 418 360 261 218 192
183 172 154 111 101 84 71 18
9
RsrII CG'GwC_CG
Cuts at: 14127 14127
Size: 15932
SacI G_AGCT'C
Cuts at: 4836 7715 8301 12347 12946 4836
Size: 2879 586 4046 599 7822
Fragments arranged by size:
7822 4046 2879 599 586
SacII CC_GC'GG
Cuts at: 7192 7192
Size: 15932
SalI G'TCGA_C
Cuts at: 3633 12969 3633
Size: 9336 6596
SanDI GG'GwC_CC
Cuts at: 212 212
Size: 15932
SapI GCTCTTCn'nnn_
Cuts at: 2300 3682 5230 5530 8617 13955 14165 2300
Size: 1382 1548 300 3087 5338 210 4067
Fragments arranged by size:
5338 4067 3087 1548 1382 300 210
SbfI CC_TGCA'GG
Cuts at: 3542 5510 5617 12979 3542
Size: 1968 107 7362 6495
Fragments arranged by size:
7362 6495 1968 107
ScaI AGT'ACT
Cuts at: 1186 5733 10540 11006 12194 15449 1186
Size: 4547 4807 466 1188 3255 1669
Fragments arranged by size:
4807 4547 3255 1669 1188 466
SexAI A'CCwGG_T
Cuts at: 1076 2111 2801 4049 1076
Size: 1035 690 1248 12959
Fragments arranged by size:
12959 1248 1035 690
SfcI C'TryA_G
Cuts at: 33 150 197 628 1945 2076 2139 2356 2992
Size: 117 47 431 1317 131 63 217 636
Cuts at: 2992 3538 5506 5613 6119 8026 8197 9082 9544
Size: 546 1968 107 506 1907 171 885 462
Cuts at: 9544 10340 10644 10653 11449 11514 11862 12059 12229
Size: 796 304 9 796 65 348 197 170
Cuts at: 12229 12424 12508 12975 13660 14653 14844 33
Size: 195 84 467 685 993 191 1121
Fragments arranged by size:
1968 1907 1317 1121 993 885 796 796
685 636 546 506 467 462 431 348
304 217 197 195 191 171 170 131
117 107 84 65 63 47 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 4378 4378
Size: 15932
SfoI GGC'GCC
Cuts at: 13364 13612 13364
Size: 248 15684
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 15932
SmaI CCC'GGG
Cuts at: 239 956 5180 7163 8963 9092 12954 239
Size: 717 4224 1983 1800 129 3862 3217
Fragments arranged by size:
4224 3862 3217 1983 1800 717 129
SmlI C'TyrA_G
Cuts at: 1716 1795 4843 5428 5518 5629 6340 6388 6523
Size: 79 3048 585 90 111 711 48 135
Cuts at: 6523 6850 7603 8014 9103 10009 10546 10936 11695
Size: 327 753 411 1089 906 537 390 759
Cuts at: 11695 11740 12115 12262 12271 12373 13087 14002 14494
Size: 45 375 147 9 102 714 915 492
Cuts at: 14494 14756 1716
Size: 262 2892
Fragments arranged by size:
3048 2892 1089 915 906 759 753 714
711 585 537 492 411 390 375 327
262 147 135 111 102 90 79 48
45 9
SnaBI TAC'GTA
Cuts at: 12924 12924
Size: 15932
SpeI A'CTAG_T
Cuts at: 67 12195 67
Size: 12128 3804
SphI G_CATG'C
Cuts at: 1320 3444 4578 7476 12985 13485 1320
Size: 2124 1134 2898 5509 500 3767
Fragments arranged by size:
5509 3767 2898 2124 1134 500
StuI AGG'CCT
Cuts at: 634 1103 2362 3703 4915 5611 12361 12430 12664
Size: 469 1259 1341 1212 696 6750 69 234
Cuts at: 12664 634
Size: 3902
Fragments arranged by size:
6750 3902 1341 1259 1212 696 469 234
69
StyI C'CwwG_G
Cuts at: 125 2339 2369 2886 3368 3497 3932 4463 4622
Size: 2214 30 517 482 129 435 531 159
Cuts at: 4622 4916 4970 6692 6734 7151 7293 7457 7467
Size: 294 54 1722 42 417 142 164 10
Cuts at: 7467 7493 8207 8439 8717 8735 9044 9206 9215
Size: 26 714 232 278 18 309 162 9
Cuts at: 9215 9275 11523 11598 12356 12624 12689 13066 13420
Size: 60 2248 75 758 268 65 377 354
Cuts at: 13420 125
Size: 2637
Fragments arranged by size:
2637 2248 2214 1722 758 714 531 517
482 435 417 377 354 309 294 278
268 232 164 162 159 142 129 75
65 60 54 42 30 26 18 10
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 345 1179 2221 2494 4433 5896 6272 6460 6648
Size: 834 1042 273 1939 1463 376 188 188
Cuts at: 6648 7065 7574 7876 8077 8947 9231 10498 12115
Size: 417 509 302 201 870 284 1267 1617
Cuts at: 12115 12135 12750 12874 13515 13648 14107 14316 345
Size: 20 615 124 641 133 459 209 1961
Fragments arranged by size:
1961 1939 1617 1463 1267 1042 870 834
641 615 509 459 417 376 302 284
273 209 201 188 188 133 124 20
TatI w'GTAC_w
Cuts at: 1022 1184 1730 1937 3651 4247 5731 7754 8462
Size: 162 546 207 1714 596 1484 2023 708
Cuts at: 8462 9548 10091 10460 10538 11004 11744 12192 12198
Size: 1086 543 369 78 466 740 448 6
Cuts at: 12198 12323 12698 15447 1022
Size: 125 375 2749 1507
Fragments arranged by size:
2749 2023 1714 1507 1484 1086 740 708
596 546 543 466 448 375 369 207
162 125 78 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 565 597 1946 1978 3613 3645 6456 6488 9326
Size: 32 1349 32 1635 32 2811 32 2838
Cuts at: 9326 9358 9433 9465 10025 10057 12873 12905 15449
Size: 32 75 32 560 32 2816 32 2544
Cuts at: 15449 15481 565
Size: 32 1016
Fragments arranged by size:
2838 2816 2811 2544 1635 1349 1016 560
75 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2044 2560 3465 7237 13729 2044
Size: 516 905 3772 6492 4247
Fragments arranged by size:
6492 4247 3772 905 516
XbaI T'CTAG_A
Cuts at: 1416 12963 1416
Size: 11547 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2607 4902 7794 8544 8733 11106 12458 13427 2607
Size: 2295 2892 750 189 2373 1352 969 5112
Fragments arranged by size:
5112 2892 2373 2295 1352 969 750 189
XhoI C'TCGA_G
Cuts at: 1716 1716
Size: 15932
XmaI C'CCGG_G
Cuts at: 237 954 5178 7161 8961 9090 12952 237
Size: 717 4224 1983 1800 129 3862 3217
Fragments arranged by size:
4224 3862 3217 1983 1800 717 129
XmnI GAAnn'nnTTC
Cuts at: 2182 2968 3181 4672 2182
Size: 786 213 1491 13442
Fragments arranged by size:
13442 1491 786 213
ZraI GAC'GTC
Cuts at: 5765 12096 5765
Size: 6331 9601
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BmtI BsiWI CspCI FseI
FspAI MfeI NdeI NheI NruI PshAI PsiI SgrAI
SrfI SspI SwaI