Restriction map for pF1KSDA1369
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        AocI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BanI        
BbeI        BbvCI       BcgI        BfrI        BlnI        
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BsrBI       BssHII      
BstAPI      BstBI       BstEII      BstPI       Bsu36I      
ClaI        CpoI        DraI        DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HaeII       HgaI        Hin1I       
HindIII     KasI        KpnI        KspI        MamI        
MluI        MroI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AflIII        705  ACATGT               A^C[GA][CT]GT
Alw44I       1315  GTGCAC               G^TGCAC
ApaI           96  GGGCCC               GGGCC^C
ApaLI        1315  GTGCAC               G^TGCAC
Asp700I       703  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1244  TGGCCA               TGG^CCA
BamHI         411  GGATCC               G^GATCC
BbrPI         232  CACGTG               CAC^GTG
BclI         1359  TGATCA               T^GATCA
BglI         1029  GCCACTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1323  AGATCT               A^GATCT
BlpI          844  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      844  GCTGAGC              GC^T[ACGT]AGC
BsaAI         232  CACGTG               [CT]AC^GT[GA]
BsaI         1710  GAGACC               GGTCTC(1/5)
Bsp1407I     1538  TGTACA               T^GTACA
BspLU11I      705  ACATGT               A^CATGT
BspMI         655  GCAGGT               ACCTGC(4/8)
BsrDI         795  GCAATG               GCAATG(2/0)
BsrFI          72  GCCGGC               [GA]^CCGG[CT]
BsrGI        1538  TGTACA               T^GTACA
BssSI         725  CACGAG               CACGAG(-5/-1)
Bst1107I      541  GTATAC               GTA^TAC
BstZ17I       541  GTATAC               GTA^TAC
CelII         844  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         72  GCCGGC               [GA]^CCGG[CT]
DraIII        690  CACCGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         594  CCTGATTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       464  CCTTGG               C^C[AT][AT]GG
FbaI         1359  TGATCA               T^GATCA
HpaI         1765  GTTAAC               GTT^AAC
MfeI         1010  CAATTG               C^AATTG
MluNI        1244  TGGCCA               TGG^CCA
MscI         1244  TGGCCA               TGG^CCA
MunI         1010  CAATTG               C^AATTG
NaeI           72  GCCGGC               GCC^GGC
NgoMIV         72  GCCGGC               G^CCGGC
NspI          705  ACATGT               [GA]CATG^[CT]
PmaCI         232  CACGTG               CAC^GTG
PmlI          232  CACGTG               CAC^GTG
PpuMI         629  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1381  GACTTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1732  CTGCAG               CTGCA^G
SalI          960  GTCGAC               G^TCGAC
SspBI        1538  TGTACA               T^GTACA
StyI          464  CCTTGG               C^C[AT][AT]GG
Tsp45I        329  GTGAC                ^GT[GC]AC
XcmI         1004  CCAGTTCAATTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          703  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          541  GTATAC               GT^[AC][GT]AC
              960  GTCGAC              
AccII          31  CGCG                 CG^CG
              693  CGCG                
AlwNI        1021  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1726  CAGCTGCTG           
BbsI          312  GTCTTC               GAAGAC(2/6)
              659  GTCTTC              
BciVI         529  GGATAC               GTATCC(6/5)
              434  GTATCC              
BfaI         1051  CTAG                 C^TAG
             1561  CTAG                
BpuAI         312  GTCTTC               GAAGAC(2/6)
              659  GTCTTC              
BseRI          37  GAGGAG               GAGGAG(10/8)
             1054  GAGGAG              
BsgI         1138  CTGCAC               GTGCAG(16/14)
             1395  CTGCAC              
BstUI          31  CGCG                 CG^CG
              693  CGCG                
BstXI         975  CCAGGAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1749  CCAAAATTCTGG        
CfoI           30  GCGC                 GCG^C
               67  GCGC                
DraII          95  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              629  AGGTCCT             
EaeI           74  CGGCCA               [CT]^GGCC[GA]
             1244  TGGCCA              
EcoO109I       95  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              629  AGGTCCT             
FauI           88  CCCGC                CCCGC(4/6)
             1200  GCGGG               
HhaI           30  GCGC                 GCG^C
               67  GCGC                
HinP1I         30  GCGC                 G^CGC
               67  GCGC                
MaeI         1051  CTAG                 C^TAG
             1561  CTAG                
MaeIII        329  GTGAC                ^GT[ACGT]AC
              485  GTTAC               
MvnI           31  CGCG                 CG^CG
              693  CGCG                
PleI          958  GAGTC                GAGTC(4/5)
             1222  GAGTC               
SspI          673  AATATT               AAT^ATT
             1339  AATATT              
TspRI         888  CACTG                CA[GC]TG[ACGT][ACGT]^
             1661  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TthHB8I     XhoII       
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