Restriction map for pF1KSDA1369
 (Circular) (Six-base) MAPSORT of: pF1KSDA1369.seq  Check: 906  from: 1  to: 5027



pF1KSDA1369  5027 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:35  ..

AccI GT'mk_AC

Cuts at:    668    1087    2065     668 
   Size:        419     978    3630

  Fragments arranged by size: 

                3630     978     419
 
Acc65I G'GTAC_C

Cuts at:   2043    2043 
   Size:       5027
 
AclI AA'CG_TT

Cuts at:   4867    4867 
   Size:       5027
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    772    1474    2852    3284    4031    4299     772 
   Size:        702    1378     432     747     268    1500

  Fragments arranged by size: 

                1500    1378     747     702     432     268
 
AflIII A'CryG_T

Cuts at:    831    2379    3483    4460     831 
   Size:       1548    1104     977    1398

  Fragments arranged by size: 

                1548    1398    1104     977
 
AgeI A'CCGG_T

Cuts at:   3415    3415 
   Size:       5027
 
AlwNI CAG_nnn'CTG

Cuts at:   1152    1857    2332    3899    1152 
   Size:        705     475    1567    2280

  Fragments arranged by size: 

                2280    1567     705     475
 
ApaI G_GGCC'C

Cuts at:    226     226 
   Size:       5027
 
ApaLI G'TGCA_C

Cuts at:   1441    3797    1441 
   Size:       2356    2671
 
ApoI r'AATT_y

Cuts at:    622    1388    1878    2031    4380    4636     622 
   Size:        766     490     153    2349     256    1013

  Fragments arranged by size: 

                2349    1013     766     490     256     153
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5027
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5027
 
AvaI C'yCGr_G

Cuts at:   2047    2047 
   Size:       5027
 
BamHI G'GATC_C

Cuts at:    537    2052     537 
   Size:       1515    3512
 
BanI G'GyrC_C

Cuts at:   2043    2457    2705    2740    2043 
   Size:        414     248      35    4330

  Fragments arranged by size: 

                4330     414     248      35
 
BanII G_rGCy'C

Cuts at:    226     977    1100    1292    1694    2041    3071     226 
   Size:        751     123     192     402     347    1030    2182

  Fragments arranged by size: 

                2182    1030     751     402     347     192     123
 
BbeI G_GCGC'C

Cuts at:   2461    2709    2461 
   Size:        248    4779
 
BbsI GAAGACnn'nnnn_

Cuts at:    431     778     431 
   Size:        347    4680
 
BciVI GTATCCnnnnn_n'

Cuts at:    571     649    2008    2925    3686    5027     571 
   Size:         78    1359     917     761    1341     571

  Fragments arranged by size: 

                1359    1341     917     761     571      78
 
BclI T'GATC_A

Cuts at:   1485    2547    1485 
   Size:       1062    3965
 
BglI GCCn_nnn'nGGC

Cuts at:   1161    1161 
   Size:       5027
 
BglII A'GATC_T

Cuts at:   1449    2542    4579    1449 
   Size:       1093    2037    1897

  Fragments arranged by size: 

                2037    1897    1093
 
BlpI GC'TnA_GC

Cuts at:    971    2139     971 
   Size:       1168    3859
 
Bme1580I G_kGCm'C

Cuts at:    226    1445    2652    2745    3801     226 
   Size:       1219    1207      93    1056    1452

  Fragments arranged by size: 

                1452    1219    1207    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2239    2320    2655    4799    2239 
   Size:         81     335    2144    2467

  Fragments arranged by size: 

                2467    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    483    3394     483 
   Size:       2911    2116
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    364    1084    1114     364 
   Size:        720      30    4277

  Fragments arranged by size: 

                4277     720      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    372    1102    1955    2203    3082    3574    3872     372 
   Size:        730     853     248     879     492     298    1527

  Fragments arranged by size: 

                1527     879     853     730     492     298     248
 
BsaI GGTCTCn'nnnn_

Cuts at:   1830    4731    1830 
   Size:       2901    2126
 
BsaAI yAC'GTr

Cuts at:    360    2019    2380     360 
   Size:       1659     361    3007

  Fragments arranged by size: 

                3007    1659     361
 
BsaBI GATnn'nnATC

Cuts at:      6    2541    4578       6 
   Size:       2535    2037     455

  Fragments arranged by size: 

                2535    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2458    2706    3408    4904    2458 
   Size:        248     702    1496    2581

  Fragments arranged by size: 

                2581    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2216    2737    3415    3689    3836    4247    2216 
   Size:        521     678     274     147     411    2996

  Fragments arranged by size: 

                2996     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    178    1195     178 
   Size:       1017    4010
 
BseYI C'CCAG_C

Cuts at:    178    2452    3210    3787    4283     178 
   Size:       2274     758     577     496     922

  Fragments arranged by size: 

                2274     922     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1249    1506    1249 
   Size:        257    4770
 
BsiEI CG_ry'CG

Cuts at:    104    2569    2615    3823     104 
   Size:       2465      46    1208    1308

  Fragments arranged by size: 

                2465    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    350     896    1100    1292    1445    1694    2041    2819    3009 
   Size:        546     204     192     153     249     347     778     190

Cuts at:   3009    3801     350 
   Size:        792    1576

  Fragments arranged by size: 

                1576     792     778     546     347     249     204     192
                 190     153
 
Bsp1286I G_dGCh'C

Cuts at:    226     350     896     977    1100    1292    1445    1694    2041 
   Size:        124     546      81     123     192     153     249     347

Cuts at:   2041    2652    2745    2819    3009    3071    3801     226 
   Size:        611      93      74     190      62     730    1452

  Fragments arranged by size: 

                1452     730     611     546     347     249     192     190
                 153     124     123      93      81      74      62
 
BspEI T'CCGG_A

Cuts at:   4247    4247 
   Size:       5027
 
BspHI T'CATG_A

Cuts at:   4203    4203 
   Size:       5027
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    772    2077    2291    2593    2974     772 
   Size:       1305     214     302     381    2825

  Fragments arranged by size: 

                2825    1305     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3319    3373    4857    5022      50 
   Size:       3269      54    1484     165      55

  Fragments arranged by size: 

                3269    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    928    2939     928 
   Size:       2011    3016
 
BsrFI r'CCGG_y

Cuts at:    198    3025    3206    3415     198 
   Size:       2827     181     209    1810

  Fragments arranged by size: 

                2827    1810     209     181
 
BsrGI T'GTAC_A

Cuts at:   1664    1664 
   Size:       5027
 
BssSI C'ACGA_G

Cuts at:    851    3298    3656     851 
   Size:       2447     358    2222

  Fragments arranged by size: 

                2447    2222     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2404    2404 
   Size:       5027
 
BstBI TT'CG_AA

Cuts at:    110    3388     110 
   Size:       3278    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1108    1882    1108 
   Size:        774    4253
 
BstYI r'GATC_y

Cuts at:      1     537    1449    1636    2052    2519    2542    2877    3123 
   Size:        536     912     187     416     467      23     335     246

Cuts at:   3123    4135    4221    4233    4579       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     912     536     467     449     416     346     335
                 246     187      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    669     669 
   Size:       5027
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1814    2977    3158    3321    1814 
   Size:       1163     181     163    3520

  Fragments arranged by size: 

                3520    1163     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2270    2270 
   Size:       5027
 
DraI TTT'AAA

Cuts at:   2026    2026 
   Size:       5027
 
DraIII CAC_nnn'GTG

Cuts at:    821     821 
   Size:       5027
 
DrdI GACnn_nn'nnGTC

Cuts at:   2733    3591    2733 
   Size:        858    4169
 
EaeI y'GGCC_r

Cuts at:    200    1370    2612    2786    3177    3204    4549     200 
   Size:       1170    1242     174     391      27    1345     678

  Fragments arranged by size: 

                1345    1242    1170     678     391     174      27
 
EagI C'GGCC_G

Cuts at:   2612    2612 
   Size:       5027
 
EarI CTCTTCn'nnn_

Cuts at:    155     657     902    1564    3050    3260     155 
   Size:        502     245     662    1486     210    1922

  Fragments arranged by size: 

                1922    1486     662     502     245     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3235    3545    3691    4837    3235 
   Size:        310     146    1146    3425

  Fragments arranged by size: 

                3425    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1098    1290    1692    2039    1098 
   Size:        192     402     347    4086

  Fragments arranged by size: 

                4086     402     347     192
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    364     772    1084    1114    1474    2852    3284    4031    4299 
   Size:        408     312      30     360    1378     432     747     268

Cuts at:   4299     364 
   Size:       1092

  Fragments arranged by size: 

                1378    1092     747     432     408     360     312     268
                  30
 
EcoNI CCTnn'n_nnAGG

Cuts at:    724     724 
   Size:       5027
 
EcoO109I rG'GnC_Cy

Cuts at:    222     756    2166     222 
   Size:        534    1410    3083

  Fragments arranged by size: 

                3083    1410     534
 
EcoRI G'AATT_C

Cuts at:   2031    2031 
   Size:       5027
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3046    3046 
   Size:       5027
 
FspI TGC'GCA

Cuts at:   2808    2808 
   Size:       5027
 
HaeII r_GCGC'y

Cuts at:   2461    2709    3731    4710    2461 
   Size:        248    1022     979    2778

  Fragments arranged by size: 

                2778    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    465     497     516     548     865     897    1639    1671    2869 
   Size:         32      19      32     317      32     742      32    1198

Cuts at:   2869    2899    2901    2931     465 
   Size:         30       2      30    2561

  Fragments arranged by size: 

                2561    1198     742     317      32      32      32      32
                  30      30      19       2
 
HincII GTy'rAC

Cuts at:   1088    1224    1893    2066    1088 
   Size:        136     669     173    4049

  Fragments arranged by size: 

                4049     669     173     136
 
HpaI GTT'AAC

Cuts at:   1893    1893 
   Size:       5027
 
KasI G'GCGC_C

Cuts at:   2457    2705    2457 
   Size:        248    4779
 
KpnI G_GTAC'C

Cuts at:   2047    2047 
   Size:       5027
 
MfeI C'AATT_G

Cuts at:   1136    1136 
   Size:       5027
 
MluI A'CGCG_T

Cuts at:   4460    4460 
   Size:       5027
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    923    1610    3698    3882     923 
   Size:        687    2088     184    2068

  Fragments arranged by size: 

                2088    2068     687     184
 
MscI TGG'CCA

Cuts at:   1372    2788    1372 
   Size:       1416    3611
 
MslI CAynn'nnrTG

Cuts at:    362     836    1273    3016    3143     362 
   Size:        474     437    1743     127    2246

  Fragments arranged by size: 

                2246    1743     474     437     127
 
MspA1I CmG'CkG

Cuts at:   1152    1281    1854    2138    2452    2812    3825    4070    1152 
   Size:        129     573     284     314     360    1013     245    2109

  Fragments arranged by size: 

                2109    1013     573     360     314     284     245     129
            
 
NaeI GCC'GGC

Cuts at:    200    3208     200 
   Size:       3008    2019
 
NarI GG'CG_CC

Cuts at:   2458    2706    2458 
   Size:        248    4779
 
NgoMIV G'CCGG_C

Cuts at:    198    3206     198 
   Size:       3008    2019
 
NspI r_CATG'y

Cuts at:    835    2080    2580    3487     835 
   Size:       1245     500     907    2375

  Fragments arranged by size: 

                2375    1245     907     500
 
PciI A'CATG_T

Cuts at:    831    3483     831 
   Size:       2652    2375
 
PfoI T'CCnGG_A

Cuts at:   1100    1100 
   Size:       5027
 
PmeI GTTT'AAAC

Cuts at:   2026    2026 
   Size:       5027
 
PmlI CAC'GTG

Cuts at:    360     360 
   Size:       5027
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1715    1747    4166    4198    1715 
   Size:         32    2419      32    2544

  Fragments arranged by size: 

                2544    2419      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    756     756 
   Size:       5027
 
PshAI GACnn'nnGTC

Cuts at:   1511    1511 
   Size:       5027
 
PspOMI G'GGCC_C

Cuts at:    222     222 
   Size:       5027
 
PstI C_TGCA'G

Cuts at:   1862    2074    2759    1862 
   Size:        212     685    4130

  Fragments arranged by size: 

                4130     685     212
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5027
 
PvuII CAG'CTG

Cuts at:   1152    1281    1854    2452    2812    1152 
   Size:        129     573     598     360    3367

  Fragments arranged by size: 

                3367     598     573     360     129
 
RsrII CG'GwC_CG

Cuts at:   3222    3222 
   Size:       5027
 
SacI G_AGCT'C

Cuts at:   1100    1292    1694    2041    1100 
   Size:        192     402     347    4086

  Fragments arranged by size: 

                4086     402     347     192
 
SalI G'TCGA_C

Cuts at:   1086    2064    1086 
   Size:        978    4049
 
SapI GCTCTTCn'nnn_

Cuts at:   3050    3260    3050 
   Size:        210    4817
 
SbfI CC_TGCA'GG

Cuts at:   2074    2074 
   Size:       5027
 
ScaI AGT'ACT

Cuts at:   4544    4544 
   Size:       5027
 
SfcI C'TryA_G

Cuts at:     33    1534    1858    1903    2070    2755    3748    3939      33 
   Size:       1501     324      45     167     685     993     191    1121

  Fragments arranged by size: 

                1501    1121     993     685     324     191     167      45
            
 
SfoI GGC'GCC

Cuts at:   2459    2707    2459 
   Size:        248    4779
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5027
 
SmaI CCC'GGG

Cuts at:   2049    2049 
   Size:       5027
 
SmlI C'TyrA_G

Cuts at:    351    1081    1970    2182    3097    3589    3851     351 
   Size:        730     889     212     915     492     262    1527

  Fragments arranged by size: 

                1527     915     889     730     492     262     212
 
SnaBI TAC'GTA

Cuts at:   2019    2019 
   Size:       5027
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5027
 
SphI G_CATG'C

Cuts at:   2080    2580    2080 
   Size:        500    4527
 
SspI AAT'ATT

Cuts at:    801    1467     801 
   Size:        666    4361
 
StyI C'CwwG_G

Cuts at:    590    2161    2515     590 
   Size:       1571     354    3102

  Fragments arranged by size: 

                3102    1571     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1663    2610    2743    3202    3411    1663 
   Size:        947     133     459     209    3279

  Fragments arranged by size: 

                3279     947     459     209     133
 
TatI w'GTAC_w

Cuts at:   1043    1664    4542    1043 
   Size:        621    2878    1528

  Fragments arranged by size: 

                2878    1528     621
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4544    4576    4544 
   Size:         32    4995
 
Tth111I GACn'n_nGTC

Cuts at:   2824    2824 
   Size:       5027
 
XbaI T'CTAG_A

Cuts at:   2058    2058 
   Size:       5027
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1137    2522    1137 
   Size:       1385    3642
 
XmaI C'CCGG_G

Cuts at:   2047    2047 
   Size:       5027
 
XmnI GAAnn'nnTTC

Cuts at:    833     833 
   Size:       5027
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstXI    BstYI  BstZ17I    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI   BssHII   BstEII   Bsu36I
     BtgI     ClaI    CspCI    EcoRV     FseI    FspAI  HindIII     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PsiI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
     SrfI     StuI     SwaI     XhoI     ZraI
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