Restriction map for pF1KSDA1369
 (Linear) (Six-base) MAPSORT of: KIAA1369.seq  Check: 6101  from: 1  to: 1890



KIAA1369.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AccI GT'mk_AC

Cuts at:      0     542     961    1890 
   Size:        542     419     929

  Fragments arranged by size: 

                 929     542     419
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646    1348    1890 
   Size:        646     702     542

  Fragments arranged by size: 

                 702     646     542
 
AflIII A'CryG_T

Cuts at:      0     705    1890 
   Size:        705    1185
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1026    1731    1890 
   Size:       1026     705     159

  Fragments arranged by size: 

                1026     705     159
 
ApaI G_GGCC'C

Cuts at:      0     100    1890 
   Size:        100    1790
 
ApaLI G'TGCA_C

Cuts at:      0    1315    1890 
   Size:       1315     575
 
ApoI r'AATT_y

Cuts at:      0     496    1262    1752    1890 
   Size:        496     766     490     138

  Fragments arranged by size: 

                 766     496     490     138
 
BamHI G'GATC_C

Cuts at:      0     411    1890 
   Size:        411    1479
 
BanII G_rGCy'C

Cuts at:      0     100     851     974    1166    1568    1890 
   Size:        100     751     123     192     402     322

  Fragments arranged by size: 

                 751     402     322     192     123     100
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     305     652    1890 
   Size:        305     347    1238

  Fragments arranged by size: 

                1238     347     305
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     445     523    1890 
   Size:        445      78    1367

  Fragments arranged by size: 

                1367     445      78
 
BclI T'GATC_A

Cuts at:      0    1359    1890 
   Size:       1359     531
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1035    1890 
   Size:       1035     855
 
BglII A'GATC_T

Cuts at:      0    1323    1890 
   Size:       1323     567
 
BlpI GC'TnA_GC

Cuts at:      0     845    1890 
   Size:        845    1045
 
Bme1580I G_kGCm'C

Cuts at:      0     100    1319    1890 
   Size:        100    1219     571

  Fragments arranged by size: 

                1219     571     100
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     357    1890 
   Size:        357    1533
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     958     988    1890 
   Size:        238     720      30     902

  Fragments arranged by size: 

                 902     720     238      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     246     976    1829    1890 
   Size:        246     730     853      61

  Fragments arranged by size: 

                 853     730     246      61
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1704    1890 
   Size:       1704     186
 
BsaAI yAC'GTr

Cuts at:      0     234    1890 
   Size:        234    1656
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52    1069    1890 
   Size:         52    1017     821

  Fragments arranged by size: 

                1017     821      52
 
BseYI C'CCAG_C

Cuts at:      0      52    1890 
   Size:         52    1838
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1123    1380    1890 
   Size:       1123     257     510

  Fragments arranged by size: 

                1123     510     257
 
BsiHKAI G_wGCw'C

Cuts at:      0     224     770     974    1166    1319    1568    1890 
   Size:        224     546     204     192     153     249     322

  Fragments arranged by size: 

                 546     322     249     224     204     192     153
 
Bsp1286I G_dGCh'C

Cuts at:      0     100     224     770     851     974    1166    1319    1568 
   Size:        100     124     546      81     123     192     153     249

Cuts at:   1568    1890 
   Size:        322

  Fragments arranged by size: 

                 546     322     249     192     153     124     123     100
                  81
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     646    1890 
   Size:        646    1244
 
BsrDI GCAATG_nn'

Cuts at:      0     802    1890 
   Size:        802    1088
 
BsrFI r'CCGG_y

Cuts at:      0      72    1890 
   Size:         72    1818
 
BsrGI T'GTAC_A

Cuts at:      0    1538    1890 
   Size:       1538     352
 
BssSI C'ACGA_G

Cuts at:      0     725    1890 
   Size:        725    1165
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     982    1756    1890 
   Size:        982     774     134

  Fragments arranged by size: 

                 982     774     134
 
BstYI r'GATC_y

Cuts at:      0     411    1323    1510    1890 
   Size:        411     912     187     380

  Fragments arranged by size: 

                 912     411     380     187
 
BstZ17I GTA'TAC

Cuts at:      0     543    1890 
   Size:        543    1347
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1688    1890 
   Size:       1688     202
 
DraIII CAC_nnn'GTG

Cuts at:      0     695    1890 
   Size:        695    1195
 
EaeI y'GGCC_r

Cuts at:      0      74    1244    1890 
   Size:         74    1170     646

  Fragments arranged by size: 

                1170     646      74
 
EarI CTCTTCn'nnn_

Cuts at:      0      29     531     776    1438    1890 
   Size:         29     502     245     662     452

  Fragments arranged by size: 

                 662     502     452     245      29
 
EcoICRI GAG'CTC

Cuts at:      0     972    1164    1566    1890 
   Size:        972     192     402     324

  Fragments arranged by size: 

                 972     402     324     192
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     646     958     988    1348    1890 
   Size:        238     408     312      30     360     542

  Fragments arranged by size: 

                 542     408     360     312     238      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     598    1890 
   Size:        598    1292
 
EcoO109I rG'GnC_Cy

Cuts at:      0      96     630    1890 
   Size:         96     534    1260

  Fragments arranged by size: 

                1260     534      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     339     371     390     422     739     771    1513    1545 
   Size:        339      32      19      32     317      32     742      32

Cuts at:   1545    1890 
   Size:        345

  Fragments arranged by size: 

                 742     345     339     317      32      32      32      32
                  19
 
HincII GTy'rAC

Cuts at:      0     962    1098    1767    1890 
   Size:        962     136     669     123

  Fragments arranged by size: 

                 962     669     136     123
 
HpaI GTT'AAC

Cuts at:      0    1767    1890 
   Size:       1767     123
 
MfeI C'AATT_G

Cuts at:      0    1010    1890 
   Size:       1010     880
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     797    1484    1890 
   Size:        797     687     406

  Fragments arranged by size: 

                 797     687     406
 
MscI TGG'CCA

Cuts at:      0    1246    1890 
   Size:       1246     644
 
MslI CAynn'nnrTG

Cuts at:      0     236     710    1147    1890 
   Size:        236     474     437     743

  Fragments arranged by size: 

                 743     474     437     236
 
MspA1I CmG'CkG

Cuts at:      0    1026    1155    1728    1890 
   Size:       1026     129     573     162

  Fragments arranged by size: 

                1026     573     162     129
 
NaeI GCC'GGC

Cuts at:      0      74    1890 
   Size:         74    1816
 
NgoMIV G'CCGG_C

Cuts at:      0      72    1890 
   Size:         72    1818
 
NspI r_CATG'y

Cuts at:      0     709    1890 
   Size:        709    1181
 
PciI A'CATG_T

Cuts at:      0     705    1890 
   Size:        705    1185
 
PfoI T'CCnGG_A

Cuts at:      0     974    1890 
   Size:        974     916
 
PmlI CAC'GTG

Cuts at:      0     234    1890 
   Size:        234    1656
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1589    1621    1890 
   Size:       1589      32     269

  Fragments arranged by size: 

                1589     269      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     630    1890 
   Size:        630    1260
 
PshAI GACnn'nnGTC

Cuts at:      0    1385    1890 
   Size:       1385     505
 
PspOMI G'GGCC_C

Cuts at:      0      96    1890 
   Size:         96    1794
 
PstI C_TGCA'G

Cuts at:      0    1736    1890 
   Size:       1736     154
 
PvuII CAG'CTG

Cuts at:      0    1026    1155    1728    1890 
   Size:       1026     129     573     162

  Fragments arranged by size: 

                1026     573     162     129
 
SacI G_AGCT'C

Cuts at:      0     974    1166    1568    1890 
   Size:        974     192     402     322

  Fragments arranged by size: 

                 974     402     322     192
 
SalI G'TCGA_C

Cuts at:      0     960    1890 
   Size:        960     930
 
SfcI C'TryA_G

Cuts at:      0    1408    1732    1777    1890 
   Size:       1408     324      45     113

  Fragments arranged by size: 

                1408     324     113      45
 
SmlI C'TyrA_G

Cuts at:      0     225     955    1844    1890 
   Size:        225     730     889      46

  Fragments arranged by size: 

                 889     730     225      46
 
SspI AAT'ATT

Cuts at:      0     675    1341    1890 
   Size:        675     666     549

  Fragments arranged by size: 

                 675     666     549
 
StyI C'CwwG_G

Cuts at:      0     464    1890 
   Size:        464    1426
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1537    1890 
   Size:       1537     353
 
TatI w'GTAC_w

Cuts at:      0     917    1538    1890 
   Size:        917     621     352

  Fragments arranged by size: 

                 917     621     352
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1011    1890 
   Size:       1011     879
 
XmnI GAAnn'nnTTC

Cuts at:      0     707    1890 
   Size:        707    1183
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI    ApaLI     ApoI    BamHI
    BanII     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BplI     BpmI    BpuEI     BsaI    BsaAI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI    BssSI    BstXI
    BstYI  BstZ17I    BtgZI   DraIII     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    Hin4I   HincII     HpaI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NspI     PciI     PfoI     PmlI
     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI     SalI
     SfcI     SmlI     SspI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BanI     BbeI    BbvCI     BcgI    BfrBI    BmgBI     BmrI
     BmtI   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI   BssHII   BstAPI    BstBI
   BstEII   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI     DrdI
     EagI     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MluI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com