Restriction map for pF1KSDA1350
 (Linear) (Six-base) MAPSORT of: KIAA1350.seq  Check: 8695  from: 1  to: 2634



KIAA1350.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AccI GT'mk_AC

Cuts at:      0    1809    2634 
   Size:       1809     825
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1569    1627    2297    2634 
   Size:       1569      58     670     337

  Fragments arranged by size: 

                1569     670     337      58
 
AflIII A'CryG_T

Cuts at:      0    1767    2634 
   Size:       1767     867
 
AleI CACnn'nnGTG

Cuts at:      0     683    2634 
   Size:        683    1951
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2083    2115    2634 
   Size:       2083      32     519

  Fragments arranged by size: 

                2083     519      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2041    2634 
   Size:       2041     593
 
ApoI r'AATT_y

Cuts at:      0     113     634    1686    1714    1891    1917    2634 
   Size:        113     521    1052      28     177      26     717

  Fragments arranged by size: 

                1052     717     521     177     113      28      26
 
BamHI G'GATC_C

Cuts at:      0    1278    2634 
   Size:       1278    1356
 
BanI G'GyrC_C

Cuts at:      0    2449    2634 
   Size:       2449     185
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     869    1784    1997    2634 
   Size:        869     915     213     637

  Fragments arranged by size: 

                 915     869     637     213
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2547    2634 
   Size:       2547      87
 
BclI T'GATC_A

Cuts at:      0     606     720    1488    2634 
   Size:        606     114     768    1146

  Fragments arranged by size: 

                1146     768     606     114
 
BfrBI ATG'CAT

Cuts at:      0     422    2461    2634 
   Size:        422    2039     173

  Fragments arranged by size: 

                2039     422     173
 
BglII A'GATC_T

Cuts at:      0    2131    2634 
   Size:       2131     503
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1022    2634 
   Size:       1022    1612
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     660     690    1621    1651    2634 
   Size:        660      30     931      30     983

  Fragments arranged by size: 

                 983     931     660      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2036    2409    2634 
   Size:       2036     373     225

  Fragments arranged by size: 

                2036     373     225
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     983    2634 
   Size:        983    1651
 
BsiHKAI G_wGCw'C

Cuts at:      0     762    2634 
   Size:        762    1872
 
BsmI GAATG_Cn'

Cuts at:      0     177     422    1891    2634 
   Size:        177     245    1469     743

  Fragments arranged by size: 

                1469     743     245     177
 
Bsp1286I G_dGCh'C

Cuts at:      0     762    2634 
   Size:        762    1872
 
BspHI T'CATG_A

Cuts at:      0    1710    2634 
   Size:       1710     924
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     495    2634 
   Size:        495    2139
 
BsrGI T'GTAC_A

Cuts at:      0    1770    2634 
   Size:       1770     864
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     428    2634 
   Size:        428    2206
 
BstYI r'GATC_y

Cuts at:      0    1278    2131    2634 
   Size:       1278     853     503

  Fragments arranged by size: 

                1278     853     503
 
BtsI GCAGTG_nn'

Cuts at:      0    1491    2613    2634 
   Size:       1491    1122      21

  Fragments arranged by size: 

                1491    1122      21
 
ClaI AT'CG_AT

Cuts at:      0    1532    2634 
   Size:       1532    1102
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     431     466    2165    2200    2634 
   Size:        431      35    1699      35     434

  Fragments arranged by size: 

                1699     434     431      35      35
 
DraI TTT'AAA

Cuts at:      0      24     587     912    2271    2634 
   Size:         24     563     325    1359     363

  Fragments arranged by size: 

                1359     563     363     325      24
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1229    2634 
   Size:       1229    1405
 
EaeI y'GGCC_r

Cuts at:      0      99    1297    2634 
   Size:         99    1198    1337

  Fragments arranged by size: 

                1337    1198      99
 
EarI CTCTTCn'nnn_

Cuts at:      0    1963    2634 
   Size:       1963     671
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1022    1569    1627    2297    2634 
   Size:       1022     547      58     670     337

  Fragments arranged by size: 

                1022     670     547     337      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1423    2634 
   Size:       1423    1211
 
EcoRI G'AATT_C

Cuts at:      0    1714    2634 
   Size:       1714     920
 
EcoRV GAT'ATC

Cuts at:      0    1557    2634 
   Size:       1557    1077
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2327    2634 
   Size:       2327     307
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     397     429     612     644     660     692    1020    1052 
   Size:        397      32     183      32      16      32     328      32

Cuts at:   1052    1621    1638    1653    1670    1829    1861    2634 
   Size:        569      17      15      17     159      32     773

  Fragments arranged by size: 

                 773     569     397     328     183     159      32      32
                  32      32      32      17      17      16      15
 
MfeI C'AATT_G

Cuts at:      0     152    2634 
   Size:        152    2482
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     664    1144    2634 
   Size:        664     480    1490

  Fragments arranged by size: 

                1490     664     480
 
MscI TGG'CCA

Cuts at:      0     101    1299    2634 
   Size:        101    1198    1335

  Fragments arranged by size: 

                1335    1198     101
 
MslI CAynn'nnrTG

Cuts at:      0     320     683    1772    2345    2634 
   Size:        320     363    1089     573     289

  Fragments arranged by size: 

                1089     573     363     320     289
 
MspA1I CmG'CkG

Cuts at:      0     515    1130    2634 
   Size:        515     615    1504

  Fragments arranged by size: 

                1504     615     515
 
NdeI CA'TA_TG

Cuts at:      0    2342    2634 
   Size:       2342     292
 
NsiI A_TGCA'T

Cuts at:      0     424    2463    2634 
   Size:        424    2039     171

  Fragments arranged by size: 

                2039     424     171
 
NspI r_CATG'y

Cuts at:      0    1771    2634 
   Size:       1771     863
 
PciI A'CATG_T

Cuts at:      0    1767    2634 
   Size:       1767     867
 
PfoI T'CCnGG_A

Cuts at:      0    1038    2634 
   Size:       1038    1596
 
PvuII CAG'CTG

Cuts at:      0     515    1130    2634 
   Size:        515     615    1504

  Fragments arranged by size: 

                1504     615     515
 
SfcI C'TryA_G

Cuts at:      0      75    2634 
   Size:         75    2559
 
SspI AAT'ATT

Cuts at:      0     651    2634 
   Size:        651    1983
 
StuI AGG'CCT

Cuts at:      0    1418    2634 
   Size:       1418    1216
 
StyI C'CwwG_G

Cuts at:      0    1820    2634 
   Size:       1820     814
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     205     986    1736    2108    2129    2634 
   Size:        205     781     750     372      21     505

  Fragments arranged by size: 

                 781     750     505     372     205      21
 
TatI w'GTAC_w

Cuts at:      0    1770    2305    2335    2634 
   Size:       1770     535      30     299

  Fragments arranged by size: 

                1770     535     299      30
 
Tth111I GACn'n_nGTC

Cuts at:      0    1467    2634 
   Size:       1467    1167
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1997    2634 
   Size:       1997     637
 
XmnI GAAnn'nnTTC

Cuts at:      0    2369    2634 
   Size:       2369     265
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AloI    AlwNI     ApoI    BamHI
     BanI     BbsI    BciVI     BclI    BfrBI    BglII     BpmI    BsaXI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrGI
   BstAPI    BstYI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EarI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI    Hin4I     MfeI
     MmeI     MscI     MslI   MspA1I     NdeI     NsiI     NspI     PciI
     PfoI    PvuII     SfcI     SspI     StuI     StyI    TaqII     TatI
  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BanII     BbeI    BbvCI     BcgI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI   DraIII     EagI     EciI  EcoICRI
 EcoO109I     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SwaI     TstI     XbaI     XhoI     XmaI
     ZraI
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