Restriction map for pF1KSDA1348
 (Circular) (Six-base) MAPSORT of: pF1KSDA1348.seq  Check: 7094  from: 1  to: 4724



pF1KSDA1348  4724 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:35  ..

AccI GT'mk_AC

Cuts at:    195    1762     195 
   Size:       1567    3157
 
Acc65I G'GTAC_C

Cuts at:   1740    1740 
   Size:       4724
 
AclI AA'CG_TT

Cuts at:   4564    4564 
   Size:       4724
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1204    2549    2981    3728    3996    1204 
   Size:       1345     432     747     268    1932

  Fragments arranged by size: 

                1932    1345     747     432     268
 
AflIII A'CryG_T

Cuts at:   1042    2076    3180    4157    1042 
   Size:       1034    1104     977    1609

  Fragments arranged by size: 

                1609    1104    1034     977
 
AgeI A'CCGG_T

Cuts at:   3112    3112 
   Size:       4724
 
AleI CACnn'nnGTG

Cuts at:    733     752    1666     733 
   Size:         19     914    3791

  Fragments arranged by size: 

                3791     914      19
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    948     948 
   Size:       4724
 
AlwNI CAG_nnn'CTG

Cuts at:    633     771     837    1485    2029    3596     633 
   Size:        138      66     648     544    1567    1761

  Fragments arranged by size: 

                1761    1567     648     544     138      66
 
ApaLI G'TGCA_C

Cuts at:   3494    3494 
   Size:       4724
 
ApoI r'AATT_y

Cuts at:    156     435     953    1728    4077    4333     156 
   Size:        279     518     775    2349     256     547

  Fragments arranged by size: 

                2349     775     547     518     279     256
 
AseI AT'TA_AT

Cuts at:     20     817      20 
   Size:        797    3927
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4724
 
AvaI C'yCGr_G

Cuts at:    254    1744     254 
   Size:       1490    3234
 
BamHI G'GATC_C

Cuts at:   1749    1749 
   Size:       4724
 
BanI G'GyrC_C

Cuts at:    258    1412    1740    2154    2402    2437     258 
   Size:       1154     328     414     248      35    2545

  Fragments arranged by size: 

                2545    1154     414     328     248      35
 
BanII G_rGCy'C

Cuts at:   1475    1514    1738    2768    1475 
   Size:         39     224    1030    3431

  Fragments arranged by size: 

                3431    1030     224      39
 
BbeI G_GCGC'C

Cuts at:   2158    2406    2158 
   Size:        248    4476
 
BbvCI CC'TCA_GC

Cuts at:   1324    1421    1324 
   Size:         97    4627
 
BciVI GTATCCnnnnn_n'

Cuts at:   2622    3383    4724    2622 
   Size:        761    1341    2622

  Fragments arranged by size: 

                2622    1341     761
 
BclI T'GATC_A

Cuts at:   1547    2244    1547 
   Size:        697    4027
 
BfrBI ATG'CAT

Cuts at:    565     565 
   Size:       4724
 
BglII A'GATC_T

Cuts at:   2239    4276    2239 
   Size:       2037    2687
 
BlpI GC'TnA_GC

Cuts at:   1298    1378    1836    1298 
   Size:         80     458    4186

  Fragments arranged by size: 

                4186     458      80
 
Bme1580I G_kGCm'C

Cuts at:    573    1415    2349    2442    3498     573 
   Size:        842     934      93    1056    1799

  Fragments arranged by size: 

                1799    1056     934     842      93
 
BmgBI CAC'GTC

Cuts at:   1392    1392 
   Size:       4724
 
BmrI ACTGGGnnnn_n'

Cuts at:    475    1150    1936    2017    2352    4496     475 
   Size:        675     786      81     335    2144     703

  Fragments arranged by size: 

                2144     786     703     675     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3091    3091 
   Size:       4724
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    192     655     889    1237     192 
   Size:        463     234     348    3679

  Fragments arranged by size: 

                3679     463     348     234
 
Bpu10I CC'TnA_GC

Cuts at:    362    1324    1421     362 
   Size:        962      97    3665

  Fragments arranged by size: 

                3665     962      97
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    679    1900    2779    3271    3569     679 
   Size:       1221     879     492     298    1834

  Fragments arranged by size: 

                1834    1221     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1449    4428    1449 
   Size:       2979    1745
 
BsaAI yAC'GTr

Cuts at:    966    1045    1716    2077     966 
   Size:         79     671     361    3613

  Fragments arranged by size: 

                3613     671     361      79
 
BsaBI GATnn'nnATC

Cuts at:      6    2238    4275       6 
   Size:       2232    2037     455

  Fragments arranged by size: 

                2232    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1145    2155    2403    3105    4601    1145 
   Size:       1010     248     702    1496    1268

  Fragments arranged by size: 

                1496    1268    1010     702     248
 
BsaWI w'CCGG_w

Cuts at:   1913    2434    3112    3386    3533    3944    1913 
   Size:        521     678     274     147     411    2693

  Fragments arranged by size: 

                2693     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   2149    2907    3484    3980    2149 
   Size:        758     577     496    2893

  Fragments arranged by size: 

                2893     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    2266    2312    3520     104 
   Size:       2162      46    1208    1308

  Fragments arranged by size: 

                2162    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    641    1097    1738    2516    2706    3498     641 
   Size:        456     641     778     190     792    1867

  Fragments arranged by size: 

                1867     792     778     641     456     190
 
BsmI GAATG_Cn'

Cuts at:   1211    1211 
   Size:       4724
 
Bsp1286I G_dGCh'C

Cuts at:    573     641    1097    1415    1475    1514    1738    2349    2442 
   Size:         68     456     318      60      39     224     611      93

Cuts at:   2442    2516    2706    2768    3498     573 
   Size:         74     190      62     730    1799

  Fragments arranged by size: 

                1799     730     611     456     318     224     190      93
                  74      68      62      60      39
 
BspEI T'CCGG_A

Cuts at:   3944    3944 
   Size:       4724
 
BspHI T'CATG_A

Cuts at:   3900    3900 
   Size:       4724
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1774    1988    2290    2671    1774 
   Size:        214     302     381    3827

  Fragments arranged by size: 

                3827     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1596    3016    3070    4554    4719      50 
   Size:       1546    1420      54    1484     165      55

  Fragments arranged by size: 

                1546    1484    1420     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    173     181    1389    1615    2636     173 
   Size:          8    1208     226    1021    2261

  Fragments arranged by size: 

                2261    1208    1021     226       8
 
BsrFI r'CCGG_y

Cuts at:    491    2722    2903    3112     491 
   Size:       2231     181     209    2103

  Fragments arranged by size: 

                2231    2103     209     181
 
BsrGI T'GTAC_A

Cuts at:   1640    1640 
   Size:       4724
 
BssSI C'ACGA_G

Cuts at:   1516    2995    3353    1516 
   Size:       1479     358    2887

  Fragments arranged by size: 

                2887    1479     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    771    1485    2101     771 
   Size:        714     616    3394

  Fragments arranged by size: 

                3394     714     616
 
BstBI TT'CG_AA

Cuts at:    110    3085     110 
   Size:       2975    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:    949     949 
   Size:       4724
 
BstYI r'GATC_y

Cuts at:      1     149    1749    2216    2239    2574    2820    3832    3918 
   Size:        148    1600     467      23     335     246    1012      86

Cuts at:   3918    3930    4276       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1600    1012     467     449     346     335     246     148
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1304    1304 
   Size:       4724
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1621    2674    2855    3018    1621 
   Size:       1053     181     163    3327

  Fragments arranged by size: 

                3327    1053     181     163
 
BtsI GCAGTG_nn'

Cuts at:    584     614    1967     584 
   Size:         30    1353    3341

  Fragments arranged by size: 

                3341    1353      30
 
DraI TTT'AAA

Cuts at:   1723    1723 
   Size:       4724
 
DrdI GACnn_nn'nnGTC

Cuts at:    557    2430    3288     557 
   Size:       1873     858    1993

  Fragments arranged by size: 

                1993    1873     858
 
EaeI y'GGCC_r

Cuts at:   2309    2483    2874    2901    4246    2309 
   Size:        174     391      27    1345    2787

  Fragments arranged by size: 

                2787    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2309    2309 
   Size:       4724
 
EarI CTCTTCn'nnn_

Cuts at:    598    2747    2957     598 
   Size:       2149     210    2365

  Fragments arranged by size: 

                2365    2149     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2932    3242    3388    4534    2932 
   Size:        310     146    1146    3122

  Fragments arranged by size: 

                3122    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1736    1736 
   Size:       4724
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    192     655     889    1204    1237    2549    2981    3728    3996 
   Size:        463     234     315      33    1312     432     747     268

Cuts at:   3996     192 
   Size:        920

  Fragments arranged by size: 

                1312     920     747     463     432     315     268     234
                  33
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1420    1420 
   Size:       4724
 
EcoO109I rG'GnC_Cy

Cuts at:    867    1163    1239    1328    1863     867 
   Size:        296      76      89     535    3728

  Fragments arranged by size: 

                3728     535     296      89      76
 
EcoRI G'AATT_C

Cuts at:    953    1728     953 
   Size:        775    3949
 
EcoRV GAT'ATC

Cuts at:   1653    1653 
   Size:       4724
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2743    2743 
   Size:       4724
 
FspI TGC'GCA

Cuts at:   2505    2505 
   Size:       4724
 
HaeII r_GCGC'y

Cuts at:   2158    2406    3428    4407    2158 
   Size:        248    1022     979    2475

  Fragments arranged by size: 

                2475    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    715     747    1544    1576    2566    2596    2598    2628     715 
   Size:         32     797      32     990      30       2      30    2811

  Fragments arranged by size: 

                2811     990     797      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1763    1763 
   Size:       4724
 
KasI G'GCGC_C

Cuts at:   2154    2402    2154 
   Size:        248    4476
 
KpnI G_GTAC'C

Cuts at:   1744    1744 
   Size:       4724
 
MluI A'CGCG_T

Cuts at:   4157    4157 
   Size:       4724
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3395    3579    3395 
   Size:        184    4540
 
MscI TGG'CCA

Cuts at:   2485    2485 
   Size:       4724
 
MslI CAynn'nnrTG

Cuts at:    566     733     752     790     947    1666    2713    2840     566 
   Size:        167      19      38     157     719    1047     127    2450

  Fragments arranged by size: 

                2450    1047     719     167     157     127      38      19
            
 
MspA1I CmG'CkG

Cuts at:    465    1182    1508    1835    2149    2509    3522    3767     465 
   Size:        717     326     327     314     360    1013     245    1422

  Fragments arranged by size: 

                1422    1013     717     360     327     326     314     245
            
 
NaeI GCC'GGC

Cuts at:   2905    2905 
   Size:       4724
 
NarI GG'CG_CC

Cuts at:   2155    2403    2155 
   Size:        248    4476
 
NgoMIV G'CCGG_C

Cuts at:   2903    2903 
   Size:       4724
 
NsiI A_TGCA'T

Cuts at:    567     567 
   Size:       4724
 
NspI r_CATG'y

Cuts at:    569    1023    1377    1558    1777    2277    3184     569 
   Size:        454     354     181     219     500     907    2109

  Fragments arranged by size: 

                2109     907     500     454     354     219     181
 
PasI CC'CwG_GG

Cuts at:    703     703 
   Size:       4724
 
PciI A'CATG_T

Cuts at:   3180    3180 
   Size:       4724
 
PflMI CCAn_nnn'nTGG

Cuts at:    289     633     837     289 
   Size:        344     204    4176

  Fragments arranged by size: 

                4176     344     204
 
PmeI GTTT'AAAC

Cuts at:   1723    1723 
   Size:       4724
 
PmlI CAC'GTG

Cuts at:    966    1045     966 
   Size:         79    4645
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3863    3895    3863 
   Size:         32    4692
 
PstI C_TGCA'G

Cuts at:   1162    1496    1771    2456    1162 
   Size:        334     275     685    3430

  Fragments arranged by size: 

                3430     685     334     275
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4724
 
PvuII CAG'CTG

Cuts at:    465    1182    2149    2509     465 
   Size:        717     967     360    2680

  Fragments arranged by size: 

                2680     967     717     360
 
RsrII CG'GwC_CG

Cuts at:   2919    2919 
   Size:       4724
 
SacI G_AGCT'C

Cuts at:   1738    1738 
   Size:       4724
 
SalI G'TCGA_C

Cuts at:   1761    1761 
   Size:       4724
 
SapI GCTCTTCn'nnn_

Cuts at:    598    2747    2957     598 
   Size:       2149     210    2365

  Fragments arranged by size: 

                2365    2149     210
 
SbfI CC_TGCA'GG

Cuts at:   1162    1771    1162 
   Size:        609    4115
 
ScaI AGT'ACT

Cuts at:    135    4241     135 
   Size:       4106     618
 
SfcI C'TryA_G

Cuts at:     33     312    1158    1492    1767    2452    3445    3636      33 
   Size:        279     846     334     275     685     993     191    1121

  Fragments arranged by size: 

                1121     993     846     685     334     279     275     191
            
 
SfoI GGC'GCC

Cuts at:   2156    2404    2156 
   Size:        248    4476
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4724
 
SmaI CCC'GGG

Cuts at:    256    1746     256 
   Size:       1490    3234
 
SmlI C'TyrA_G

Cuts at:    694    1879    2794    3286    3548     694 
   Size:       1185     915     492     262    1870

  Fragments arranged by size: 

                1870    1185     915     492     262
 
SnaBI TAC'GTA

Cuts at:   1716    1716 
   Size:       4724
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4724
 
SphI G_CATG'C

Cuts at:   1777    2277    1777 
   Size:        500    4224
 
StyI C'CwwG_G

Cuts at:    996    1858    2212     996 
   Size:        862     354    3508

  Fragments arranged by size: 

                3508     862     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    279     716    2307    2440    2899    3108     279 
   Size:        437    1591     133     459     209    1895

  Fragments arranged by size: 

                1895    1591     459     437     209     133
 
TatI w'GTAC_w

Cuts at:    133     821    1640    4239     133 
   Size:        688     819    2599     618

  Fragments arranged by size: 

                2599     819     688     618
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4241    4273    4241 
   Size:         32    4692
 
Tth111I GACn'n_nGTC

Cuts at:   2521    2521 
   Size:       4724
 
XbaI T'CTAG_A

Cuts at:   1755    1755 
   Size:       4724
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    480    1012    2219     480 
   Size:        532    1207    2985

  Fragments arranged by size: 

                2985    1207     532
 
XmaI C'CCGG_G

Cuts at:    254    1744     254 
   Size:       1490    3234
 
XmnI GAAnn'nnTTC

Cuts at:    380     380 
   Size:       4724
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NsiI     NspI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI     BcgI     BglI     BmtI    BsaXI    BseRI
     BsgI    BsiWI    BsmBI   BssHII   BstEII  BstZ17I     BtgI     ClaI
    CspCI   DraIII     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NdeI     NheI     NotI     NruI     PacI     PfoI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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